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김온유(On You Kim),이다영(Da Young Lee),이승연(Seung Yun Lee),강지협(Ji Hyeop Kang),김재현(Jae Hyeon Kim),김현우(Hyun Woo Kim),오동훈(Dong Hoon Oh),정재원(Jae Won Jeong),허선진(Sun Jin Hur) 한국축산식품학회 2021 Food and Life Vol.2021 No.2
This review provides an overview of the use of pig by-products such as bile salt and their originated bioactive compounds. Pig by-products also increase in production as consumption of meat increases every year, but due to the high cost of processing and the large imbalance in supply and demand over the season, they also change significantly and rely on imported by-products with low price fluctuations. For this reason, a large amount of domestic pork by-products that are not consumed are being discarded. Several studies have conducted to developing the bioactive material such as chenodeoxycholic acid (CDCA) and synthesis of ursodeoxycholic acid (UDCA) from pig by-products. CDCA can be extracted from bile salt in mammals and used as precursors to synthesize UDCA. UDCA is well known as the gallbladder component of bear, and it is used as an improvement in liver function in Korea and the eastern countries. Therefore, gallbladder, which is a by-roduct of pig slaughter, will be available as a material for acquiring bioactive compounds such as UDCA.
이영현 ( Young Hyun Lee ),김대성 ( Dae Sung Kim ),김원 ( Won Kim ),김용기 ( Yong Ki Kim ),엄신 ( Eom Shin ),박현진 ( Hyun Jin Park ),이은용 ( Eun Yong Lee ),김온유 ( On You Kim ),이동원 ( Dong Won Lee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
The aim of this study is to overcome solubility of poorly water soluble drugs. We prepared solid dispersion containing nanoparticle of improvement of dissolution property pranlukast by using spray dryer. This solid dispersions formulated to improvement of dissolution using eudragit(R) L100 as a water soluble for the solid dispersion using poloxamer as a surfactant. Characterization of pranlukast solid dispersion analyzed by particle size analyzer, DSC, XRD, TGA and FT-IR. particle size analyzer was used to investigate size of pranlukast in solid dispersions. DSC and XRD were used to analyze the amorphous of solid dispersions. TGA was used to analyzer the changed weight of pranlukast and solid dispersions. FT-IR was used to analyzer the salt formation by hydrogen bond between pranlukast and eudragit(R) L100. The in vitro test was carried out to find about improvement of dissolution rate of pranlukast solid dispersion in intestinal juice(pH 6.8), controlled experiment compared pranlukast solid disperion with conventional drugs(Onon(R)). This studies displayed improvement of dissolution of prepared solid dispersion by using spray drying, poorly water soluble of pranlukast as orally pharmaceutical formulation.
다공크기에 따라 수핵세포가 파종된 PLGA 지지체의 압축강도에 관한 연구
홍희경 ( Hee Kyung Hong ),김순희 ( Soon Hee Kim ),이선경 ( Seon Kyoung Lee ),이영현 ( Young Hyun Lee ),김수진 ( Soo Jin Kim ),김온유 ( On You Kim ),이동원 ( Dong Won Lee ),이종문 ( John M. Rhee ),손영숙 ( Young Sook Son ),강길선 ( 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Tissue engineering has the possible to improve upon current techniques for intervertebral disc repair. A key component in tissue engineering for disc regeneration is the scaffold that serves as a template for cell interaction and the formation of extracellular matrix to provide structural support to the newly formed tissue. Recently developed scaffolds, Poly(lactide-co-glycolide)(PLGA) most widely used due to its advantages such as good biocompatibility, controllable rate of degradation and metabolizable degradation products. We measured compression strength of scaffolds with various pores to select suitable pore for tissue-engineering bio-disc. PLGA scaffolds were prepared by solvent casting/salt-leaching. NP cell were harvested from the disc of adult female rabbit. These cells were seeded in prepared PLGA scaffold and cultured in DMEM mdeium supplimented, 10% FBS, 1% antibiotic antinycotics for 1, 4, 6 weeks. We Morphology of cellular adhesion and extracellular matrix production were confirmed by scanning electron microscope(SEM). According to our result, the compression strength of scaffold decreased with increasing porogen size.
실크/PLGA 필름에서 실크 함량이 망막색소 상피세포의 부착 및 증식 거동에 미치는 영향
조은혜(Eun Hye Jo),김수진(Soo Jin Kim),조수진(Su Jin Cho),이가영(Ga Young Lee),김온유(On You Kim),이은용(Eun Yong Lee),조원형(Won Hyung Cho),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2011 폴리머 Vol.35 No.4
망막 조직공학을 위한 생체 재료는 기계적 안전성, 생체적합성, 낮은 분해속도 등을 포함하여 in vivo에서 잠재적인 유용성을 위한 몇 가지 중요한 특징이 입증되어야 한다. 실크 필름 생체재료는 이러한 기능적인 요구에 맞게 디자인되었다. 0, 10, 20, 40, 및 80 wt%의 실크가 함유된 천연/합성물질과 하이브리드화된 silk/PLGA 필름을 용매 증발법으로 제조하였다. 1, 2, 및 3일 후에 부착된 세포 수를 확인하기 위해 MTT 분석을 하였고 SEM을 통해 필름에 부착된 세포 모폴로지를 확인하였다. 또한, mRNA 발현정도를 알아보기 위해 retinal pigment epithelium(RPE) 세포의 프라이머인 RPE65를 사용하여 RT-PCR을 실시하였고 RPE 세포의 특정 단백질인 cytokeratin의 발현을 확인하고 세포의 증식을 비교하기 위해 면역화학염색을 실시하였다. 본 실험을 통해 실크/PLGA 필름에서 20∼40 wt%실크를 함유한 경우에 RPE 세포의 부착과 증식에 가장 좋은 영향을 미치는 것을 확인하였다. Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility, including mechanical integrity, biocompatibility, and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40, and 80 wt% of silk by a solvent evaporation method. MTT assay was used to confirm the number of cells attached on film at 1, 2, and 3 days, respectively. The morphology of cellular adhesion on films was also confirmed by scanning electron microscope (SEM). RT-PCR was conducted to confirm mRNA expression of retinal pigment epithelium(RPE) using RPE65 as a RPEs marker and the expression of cytokeratin were determined by immunofluorescence staining. We confirmed that the silk/PLGA film of 20∼40 wt% silk was superior for the adhesion and proliferation of RPEs.
히알루론산을 함유한 PLGA 지지체와 섬유륜세포를 이용한 디스크 재생
최진희 ( Jin Hee Choi ),김순희 ( Soon Hee Kim ),홍희경 ( Hee Kyung Hong ),김수진 ( Soo Jin Kim ),이선경 ( Seon Kyoung Lee ),김온유 ( On You Kim ),박종학 ( Jong Hak Park ),이종문 ( John M Rhee ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
Tissue engineering strategies have the potential to improve upon current techniques for intervertebral disc repair. However, determining a suitable biomaterial scaffold for disc regeneration is difficult due to the complex fibrocartilaginous structure of the tissue. Poly(lactide-co-glycolide)(PLGA) has controllable biodegradability and good biocompatibility. hyaluronic acid(HA) called natural moisturizer can be used as biomaterials. In this study, we evaluated 3-dimensional HA loaded PLGA scaffold on proliferation and phenotype maintenance of annulus fibrosus cells. HA/PLGA scaffolds were prepared by emulsion freeze-drying method. Annulus fibrosus cells were seeded in HA/PLGA scaffold, and then cell viability and proliferation analyzed by 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide(MTT) assay. Reverse transcription polymerase chain reaction(RT-PCR) was assessed to measure mRNA expression for type II collagen, aggrecan and glycosaminoglycan(GAG) quantity from annulus fibrosus cells in scaffolds. In MTT assay result, cell viability in HA/PLGA scaffolds were higher than PLGA scaffolds. In addition, annulus fibrosus strongly expressed their specific mRNA and produced well sGAG in HA/PLGA scaffold. This result indicates that HA/PLGA scaffold is useful for intervertebral disc regeneration.
이종 췌도 이식을 위한 Ba²+-알진산 미세피막화 췌도 형성
박헌석 ( Heon Seok Park ),함동식 ( Dong Sik Ham ),유영혜 ( Young Hye You ),신주영 ( Ju Young Shin ),김지원 ( Ji Won Kim ),조재형 ( Jae Hyoung Jo ),김온유 ( On You Kim ),강길선 ( Gil Son Khang ),윤건호 ( Kun Ho Yoon ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.5
Islet transplantation is one of the promising treatment strategies for the cure of diabetes. However, the hurdles such as shortage of human tissue, immune rejection and recurrence of autoimmunity should be overcome for successful islet transplantation. Xeno-transplantation can be a solution to shortage of human sources. Immune isolation by microencapsulation of islet with alginate polymer could escape the immune injuries. Microencapsulated xenoislet transplantation is a one of the ideal strategies to cure the diabetes. Here, we introduce microencapsulation of islets for successful xenogenic islet transplantation. We achieved optimization of encapsulating condition. First, we demonstrated viability and insulin secreting capacity of encapsulated islets in vitro. We confirmed their ability of maintaining normoglycemia and escaping immune rejection after rat or porcine islet transplantation to diabetic mouse models.
실크/PLGA 하이브리드 지지체의 기계적 물성과 세포친화력
송이슬(Yi Seeul Song),유한나(Han Na Yoo),엄신(Shin Eum),김온유(On You Kim),유석철(Suk Chul Yoo),김형은(Hyung Eun Kim),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2011 폴리머 Vol.35 No.3
조직 형성을 위한 생리학적 환경을 제공하는 새로운 생활성 지지체의 디자인은 생체재료 연구에서 중요한 분야이다. 본 연구에서는 3차원적 실크/PLGA 지지체의 물성평가를 위해 압축강도, 수분친화도, SEM 분석을 하였으며, 세포친화성 평가를 위해 RAW 264.7과 NIH/3T3의 부착, 증식 및 표현형유지와 염증반응에 미치는 영향을 조사하였다. 지지체는 용매 캐스팅/염 추출법으로 제조하였고, 압축강도, 수분친화도 면에서 실크 함량이 20 wt%에서 우수함을 확인했으며, 표면의 거침도를 높여 세포부착에 긍정적인 구조임을 확인하였다. 세포친화성 분석 결과, 실크함량이 20 wt%인 실크/PLGA 지지체에서 높은 초기부착도 및 증식률을 보였으며, 실크함량이 20 wt%에서 염증반응이 눈에 띄게 감소함을 확인하였다. 조직공학적 응용에 실크/PLGA 지지체가 유용할 것이라 판단하였다. The design of new bioactive scaffolds offering physiologic environment for tissue formation is an important frontier in biomaterials research. In this study, we performed compressive strength, water-uptake ability, and SEM analysis for physical property assessment of 3-D silk/PLGA scaffold, and investigated the adhesion, proliferation, phenotype maintenance, and inflammatory responses of RAW 264.7 and NIH/3T3 for cell compatibility. Scaffolds were prepared by the solvent casting/salt leaching method and their compressive strength and water-uptake ability were excellent at 20 wt% silk content. Result of cell compatibility assay showed that inflammatory responses distinctly decreased, and initial adhesion and proliferation were maximized at 20 wt% silk content. In conclusion, we suggest that silk/PLGA scaffolds may be useful to tissue engineering applications.