원자로 보호계통 데이터 통신망의 설계 적합성 분석

김영두(Young doo Kang),고정수(Jung soo koh),구철수(Cheol Soo Goo),김복렬(Bok ryul Kim) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7

서남부간척지 벼 담수표면직파 재배시 기비시용시기별 생육 및 수량

김영두 ( Young Doo Kim ),최민규 ( Min Kyu Choi ),백만기 ( Man Gee Baek ),구본일 ( Bon Il Ku ),강신구 ( Shin Gu Kang ),박홍규 ( Hong Kyu Park ),최원영 ( Weon Young Choi ),고재권 ( Jae Kwon Ko ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.4

Field experiment was carried out to identify the optimum time of basal application of nitrogen in Southwestern reclaimed saline land. Nitrogen application before seeding and 10, 20 & 30 days after seeding were evaluated with three nitrogen application levels(140, 170 & 200 kg/ha) in Munpo soil series having 0.1~0.2% of soil salinity. Nitrogen was split-applied with forestage; basal, tillering stage, flowering initiated stage and ripening stage by a ratio of 50-20-20-10%. Results reveled that seedling stands were considerably higher when basal nitrogen applied before seeding as compared to after seeding. Grain yield increased with increase of nitrogen application before seeding. Grain yield was the highest when nitrogen application was 170~200 kg/ha before seeding. Therefore, it is recommended that the optimum nitrogen application level was 170~200 kg/ha before seeding in direct seeded rice in southwestern reclaimed saline land for maximum plant establishment and grain yield.

남부평야지에서 고품질 쌀 생산을 위한 "수광"의 적정 이앙시기

김영두 ( Young Doo Kim ),강신구 ( Shin Gu Kang ),구본일 ( Bon Il Ku ),상완규 ( Wan Gyu Sang ),조서리 ( Seo Ri Jo ),박홍규 ( Hong Kyu Park ),신운철 ( Woon Chul Shin ),고종철 ( Jong Cheol Ko ),양운호 ( Woon Ho Yang ),김보경 ( Bo Kye 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.1

A field experiment was conducted to determine the optimal transplanting date for ‘Sukwang’, a high grain quality japonica rice, in the southwestern plain area in South Korea. 25-day-old seedlings were transplanted four times by 15 days interval on May 16, June 1, 16, and 30 in 2013 and 2014. Heading dates were August 12 and August 29 in the first and the last transplanting, respectively. Ripening ratio was highest in June 1 and June 16 plots at range of 93 - 94%, and was lowest in 16 May plot. Milled rice yield was the highest in June 1 and June 16 at rage of 538 - 555 kg/10a, and was the lowest in May 16. Head rice yield showed same tendency to milled rice yield. Protein content was not significantly different among treatments except June 1, however, it was slightly increased in the later transplanting plots. Amylose content was increased in the later transplanting plots. According to the regression curve of transplanting dates and grain yield, the optimal transplanting date of ‘Sukwang’ was June 10 in the southwestern plain area in Korea.

연구논문 : 자연과학; 벼 포트묘의 이모작 이앙시기별 생육 및 수량변화

김영두 ( Young Doo Kim ),강신구 ( Shin Gu Kang ),구본일 ( Bon Il Ku ),최민규 ( Min Kyu Choi ),박홍규 ( Hong Kyu Park ),상완규 ( Wan Gyu Sang ),이민희 ( Min Hee Lee ),김보경 ( Bo Kyeong Kim ) 한국국제농업개발학회 2013 韓國國際農業開發學會誌 Vol.25 No.2

청보리와 밀을 재배한 후 벼 이앙시기를 6월 10일 및 6월 25일로 달리하여 포트육묘의 재배적인 효과를 검토하고자 친농 품종을 공시하여 수행한 결과를 요약하면 다음과 같다. 이앙시기별 생육상황을 보면 경직경은 두 시기 모두 관행재배보다 포트재배가 굵었으며, 초장 및 유효경비율도 같은 경향이었으나, LAI, 완전미비율 및 단백질함량은 이앙시기간 차이가 인정되었다. 간장, 수장 및 등숙비율은 두 시기 모두 관행재배보다 포트재배에서 컸으나, 수수는 관행재배가 포트재배 보다 많았고, 천립중은 비슷한 경향이었다. 백미수량은 6월 10일 이앙에서는 관행재배보다 포트재배가 544 kg/10a, 6월 25일 이앙에서 513 kg/10a로 관행재배보다 각각 4%로 증수하였다. 수량과 수량구성요소와의 상관관계를 보면 두 시기 모두 관행재배는 수수 및 등숙비율에서, 포트재배는 1수립수에서 정의 상관이 인정되었다. 또한 기여도를 보면 두 시기 모두 관행재배는 수수 및 천립중이, 포트재배는 1수립수 및 수수의 기여도가 높았다. 주간과 분얼간 분포도는 두 시기 모두 관행재배는 1차, 주간, 2차 분얼 순이었고, 포트재배는 1차 분얼이 많았으나 주간이나 2차 분얼은 비슷하였다. The healthy rice seedling (Oriza sativa L.) for optimal yield in rice-winter cereals double cropping system is required to enhance the agronomic and economic sustainability of crop production in the southern plain area of Korea. Performance of the pot seedling and conventional tray seedling (control) methods using Chinnongbyeo was compared at two transplanting times of June 10 and June 25 in 2011 following forage barley and wheat cultivation, respectively. Culm diameter, plant height, and productive tillering ratio were higher in pot seedling in both transplanting times than those of the conventional tray seedling. However, leaf area index (LAI), perfect grain ratio, and protein content in milled rice were different by transplanting time. Milled rice yields of pot seedling were 544 kg/10a in June 10 and 513 kg/10a in June 25, respectively, which were 4% higher than the tray seedling. In both transplanting time, panicle number and ripened grain ratio were correlated with milled rice yield in conventional tray seedling, and number of spikelet per panicle in pot seedling. Path coefficients showed higher direct effect of panicle number and 1000-grain weight on yield in tray seedling, and spikelet number per panicle and panicle number in pot seedling. Number of tillers of each order in tray seedling for both transplanting time were different with the order of primary tiller, main stem, and secondary tiller. Pot seedling had more primary tiller than main stem and secondary which were similar in both transplanting time.

Koji 원료미의 품종에 다른 양조 특성

김영두(Kim Young Doo),하기용,(Ha Ki Yong),조수연(Cho Soo Yeon),강춘식(Kang Choon Sik) 한국육종학회 1999 한국육종학회지 Vol.31 No.4

밀양23호와 기호벼 교잡 재조합자식계통(RILs)의 품질관련 특성 변이

강현중,김영두,김현순,이영태,은무영,Kang, Hyeon-Jung,Kim, Young-Doo,Kim, Hyun-Soon,Lee, Young-Tae,Eun, Moo-Young 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

통일형인 밀양23호와 자포니카인 기호벼를 교잡한 재조합자식계통을 대상으로 품질관련 특성 변이를 살펴본 결과 공시계통들에 대한 품질 관련 형질들의 변이 분포는 매우 폭 넓고 다양했으며 대부분의 형질에서 연속적인 정규분포를 보였다. 조사된 형질간의 상관관계는 립의 두께에 대하여 립폭, 심백과 복백은 고도의 정의 상관관계를 나타냈으나 심백과 알카러 붕괴도는 고도의 부의 상관관계를 보였다. 단백질 함량과는 아밀로스, Mg/K 비율에서 고도의 부의 상관을 나타냈으며, K와 지방 함량과는 고도의 정의 상관을 보였다. 밥의 물리성에서는 딱딱한 정도를 나타내는 경도와 부착성, 탄력성, 검성, 저작성에서 고도의 정의 상관을 보였다. The rice quality related traits including physico-chemical traits were evaluated with one hundred sixty-four MG RILs derived from the cross between Milyang 23, Indica/Japonica hybrid type, and Gihobyeo, Japonica type. The variation distribution of all traits examined approximately fit normal distribution and transgressive segregants over parents were observed for all traits. The occurrence of such transgression could be associated with the interactions of complementary QTL alleles from two parents. However in this study, it could not be concluded that our results of segregation were due to either complementary gene effects or overdominance of a major gene. These factors should be verified by further studies. Correlations between traits were evaluated by regressing phenotypic values of one trait on those of another traits. There are highly significant correlation between grain thickness with grain width, white core and white belly. But between white belly and alkali digestion value showed highly negative significant correlation. Contents of protein showed highly negative correlation with amylose and Mg/K ratio but showed highly correlation with K and Fat contents. Hardness of cooked rice showed highly correlation with adhesiveness, elasticity, gumminess, chewiness.

바이오센서 개발을 위한 발광 유전자의 대장균에서의 발현 시스템 분석

조미미,김영두,강경숙,김숙경,양인철,박상열,이찬용,Cho, Mi-Mi,Kim, Young-Doo,Kang, Kyung-Sook,Kim, Sook-Kyung,Yang, In-Chul,Park, Sang-Ryoul,Lee, Chan-Yong 한국미생물학회 2009 미생물학회지 Vol.45 No.4

발광 세균 Photobacterium과 Vibrio의 lux 유전자가 삽입된 재조합 플라스미드를 유전자 전이시킨 발광표현형 대장균이 내는 빛의 세기를 조사하였다. 여러 대장균 균주에 형질전환 시켰는 바, 빛을 내는데 관여하는 효소들을 코드하는 유전자를 모두 포함하는 Photobacterium leiognathi lux 오페론이 삽입된 재조합플라스미드(PlXba.pT7-3)가 형질전환된 대장균 43R (Escherichia coli 43R) 균주에서는 발광세기가 다른 균주에 비해 1,000배 이상 되었으며, Vibrio harveyi luxA 유전자와 luxB 유전자를 융합시킨 유전자가 삽입된 재조합플라스미드(VhluxAB.pT7-5)가 형질전환 된 대장균 43R (E. coli 43R)에서는 기질인 fatty aldehyde를 가하면 단일 콜로니에서도 빛을 볼 수 있게 되는 대장균 형질전환체를 얻었다. 또한 이들이 중금속에 노출되었을 때 생물발광이 감소하였으며, 이들 발광 대장균 형질 전환체가 담긴 고정화된 세포에서도 빛을 내는 것을 관측함으로써 이들 실험 결과들이 lux 유전자를 활용한 바이오센서 시스템 개발의 기반 기술이 될 가능성을 보였다. To provide the basis of biosensor based on the lux genes from bioluminescent bacteria of Photobacterium leiognathi and Vibrio harveyi, we test the expression of lux genes in several strains of Escherichia coli. The expression of the recombinant plasmid of PlXba.pT7-3, containing all lux genes requiring for light emission without adding substrate, in E. coli 43R was so strong to see the blue-green light in single colony as well as in the alginate immobilized cell. In addition, the light intensity was decreased by adding heavy metal ion such as cadmium and zinc ions. These result raise the possibility that a biosensor can be developed using the lux genes system.

Construction, Expression, and Purification of N-Terminal Variants of Lumazine Protein from Photobacterium leiognathi

강경숙,김소영,최지선,김영두,로버트 포쿠,남기석,이찬용,Kang, Kyoung-Suk,Kim, So-Young,Choi, Ji-Sun,Kim, Young-Doo,Pokoo, Robert,Nam, Ki-Seok,Lee, Chan Yong The Microbiological Society of Korea 2013 미생물학회지 Vol.49 No.2

루마진 단백질은 발광 세균인 Photobacterium 종에서 추출된 형광성 단백질이다. 형광성을 지닌 최소 크기의 Photobacterium leiognathi 야생형 아미노-말단 도메인 루마진 단백질(N-terminal domain of lumazine protein 118 wt)과 여러 영역에 tryptophan을 생성시킨 돌연변이 단백질들(N-LumP 118 V41W, S48W, T50W, D64W, A66W)을 코드하는 유전자들을 위치 지정 돌연변이(Site Directed Mutagenesis)와 중합효소 연쇄 반응(Polymerase Chain Reaction)을 통해 제조하였다. 위의 유전자들이 포함된 재조합 플라스미드를 대장균에 형질 전환시켜 과발현시키는 최적의 조건을 찾았으며, 발현된 야생형 및 돌연변이 아미노-말단 영역 루마진 단백질을 6X-His tag system을 이용하여 정제 하였다. 흡광 및 형광 분광광도계를 이용한 실험 결과 이들 단백질들은 리간드인 6,7-dimethyl-8-ribityllumazine과 결합하여 형광성을 보유함을 보였다. 따라서 이들은 형광성을 지니게 되는 최소 크기의 루마진 단백질일 뿐만 아니라 형광성을 지닌 아미노산인 tryptophan이 여러 위치에 유일하게 존재함으로써 배향성 및 거리 등의 단백질의 구조 및 결합에 관한 심도 있는 연구에 탐침자로써 유용하게 활용 될 수 있을 것이다. Lumazine protein is a fluorescent protein isolated from the bioluminescent bacteria of Photobacterium species. To generate minimal size of lumazine protein with possessing fluorescent characteristic, the gene coding for the wild type N-terminal domain of lumazine protein (N-LumP 118) containing amino acids up to 118 from Photobacterium leiognathi was produced. In addition, the genes coding for the variant proteins of N-LumP 118, replaced with one tryptophan amino acid (N-LumP 118 V41W, S48W, T50W, D64W, and A66W), were also constructed by Polymerase Chain Reaction and Site Directed Mutagenesis. These proteins were expressed in Escherichia coli by transformation with recombinant plasmids and purified by 6X-His tagging system. Spectroscopic studies have show that the purified proteins are capable of binding to the fluorescent ligand 6,7-dimethyl-8-ribityllumazine, resulted in showing of fluorescent characteristic with the minimal size of protein. From these studies, the mutant proteins containing single tryptophan amino acid residue, possessing its own intrinsic flouophore character at the different position, will be able to the use as a probe for further studies to deduce their three dimensional structure and the binding modes.

호남평야지 청보리-벼 이모작에서 벼 폿트묘 적정 재식밀도 구명

강신구 ( Shin Gu Kang ),김영두 ( Young Doo Kim ),구본일 ( Bon Il Ku ),상완규 ( Wan Gyu Sang ),이민희 ( Min Hee Lee ),박홍규 ( Hong Kyu Park ),이점호 ( Jeom Ho Lee ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.2

본 시험은 호남평야지에서 청보리-벼 이모작 재배시 벼폿트묘에 알맞은 적정 재식밀도를 구명하기 위하여 수행하였다. 관행산파묘와 폿트묘를 이용하여 재식밀도별 생육상황을 보면 경직경은 관계없이 관행보다 폿트묘에서 굵었고, 출수기는 폿트묘 이앙구에서 50주~70주/3.3m2가 관행보다 2일, 80주/3.3m2가 관행보다 1일 정도 빨랐다. 간장은 관행산파묘보다 폿트묘의 간장이 더 컸고, 재식밀도별로는 재식밀도가 낮을수록 간장이 더 크게 나타났으나, 이삭길이는 재식밀도별로 유의한 차이를 보이지 않았다. 이삭수는 재식밀도가 높아질수록 단위면적당 이삭수가 많아지는 경향이었다. 등숙비율 및 천립중은 육묘방법 및 재식밀도 사이에 유의한 차이가 없었다. 백미수량은 재식밀도별로 보면 폿트묘 60주/3.3m2가 543kg/10a로 관행 518kg/10a 보다5% 증수하였다. 완전미 비율은 폿트묘 80주/3.3m2 이앙구에서 다소 낮았고, 육묘방법간에 차이는 없었으나 재식밀도 간에는 다소 차이가 있었던 반면, 아밀로스 함량은 재식밀도 간에 유의한 차이가 없었다. This experiment was carried out to investigate the optimum planting density for rice pot seedling cultivation by machine transplanting in forage barley-rice double cropping system in Honam plain area. Field experiment was conducted at research field of NICS in Iksan in 2012 and 2013. Rice seedling of Chinnongbyeo (mid-late maturing) was raised for 30 days in pot-seedling tray (448 holes) and conventional tray, and transplanted on June 10 in both years. Planting densities of pot seedling were 50, 60, 70, and 80 hills/3.3m2. Conventional tray seedling was transplanted as control at a planting density of 80 hills/3.3m2 on June 10. Tiller number per m was increased as planting density increasing, but stem diameter was decreased. Heading dates of pot seedling plots were August 19 except 80 hills/3.3m2, which were 2 days earlier than the control. Culm length, number of panicle, panicle length, and ripening grain ratio were higher in pot seedling compared to the controls, but 1000-grain weight showed no significant difference. Milled rice yield was significantly different between planting densities. The highest grain yield was 543kg/10a in 60 hills/3.3m2 of pot seedling, and it was 5% higher than its control (518kg/10a). Head rice ratios of pot seedlings and the controls were not significantly different in both transplanting dates.

연구보문 : 자연과학; 벼 재배양식간 간단관개에 따른 물 절약 효과

강신구 ( Shin Gu Kang ),구본일 ( Bon Il Ku ),박홍규 ( Hong Kyu Park ),이경보 ( Kyeong Bo Lee ),최민규 ( Min Kyu Choi ),상완규 ( Wan Gyu Sang ),김영두 ( Young Doo Kim ),김보경 ( Bo Kyeong Kim ) 한국국제농업개발학회 2013 韓國國際農業開發學會誌 Vol.25 No.3

To verify the effect of intermittent irrigation on water saving, two irrigation regimes, intermittent irrigation (II) and conventional irrigation (CI), were applied to machine transplanting (TP) and wet-hill-seeding (WHS) in 2009 and 2010. In intermittent irrigation plots, alternate flooding was applied from 20 days after transplanting and from 30 days after direct seeding. Conventional irrigation was imposed by continuous flooding including broken irrigation. The amount of irrigation water in II plots was 22% and 24% lower than that in CI plots of machine transplanting and wet-hill-seeding cultivation, respectively. Water productivity of intermittent irrigation plots ranged from 0.73 and 0.79. There were no significant different in plant growth and milled rice yield between the irrigation methods.