IL-15에 의한 류마티스관절염 환자 활막 섬유모세포에서의 SDF-1 유도

박영은 ( Young Eun Park ),김성일 ( Sung Il Kim ),박성후 ( Seong Hu Park ),백승훈 ( Seung Hoon Baek ),오혜좌 ( Hye Jwa Oh ),허양미 ( Yang Mi Heo ),조미라 ( Mi La Cho ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.3

Objective: Interleukin-15 (IL-15) recruits and activates synovial T cells, and IL-15 plays an important role in amplifying and perpetuating inflammation in the pathogenesis of rheumatoid arthritis (RA). Stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant for memory T cells in the inflamed RA synovium. This study investigated the effect of IL-15 on SDF-1 production in RA fibroblast-like synoviocytes (FLS). Methods: The expressions of IL-15 and SDF-1 were determined from the synovium of patients with RA and osteoarthritis (OA) by performing immunohistochemistry. The expressions of SDF-1 was measured from the RA FLS that were cultured with IL-15 and IL-17 by real-time RT-PCR and ELISA. The SDF-1 expression was also measured, via ELISA, from the RA FLS stimulated by IL-15 together with the inhibitors of such intracellular signal molecules as phosphatidylinositol 3-kinase (PI 3-kinase, LY294002), STAT3 (AG490), MAP Kinase (PD98059), NF-κB (parthenolide) and activator protein 1 (AP-1, curcumin). Results: IL-15 and SDF-1 were mainly expressed in the RA synovium compared to that of the OA synovium. IL-15 increased the SDF-1 expressions and it, and had an additive effect with IL-17 on the SDF-1 expressions in the cultured RA FLS. The IL-15 induced increase of the SDF-1 expression in the cultured RA FLS was blocked by the inhibitors of PI 3-kinase, NF-κB and AP-1. Conclusion: The SDF-1 expression was increased in the RA synovium and it was up-regulated by IL-15 in the RA FLS through the PI 3-kinase, NF-κB, and AP-1 pathways. These results imply that the IL-15 induced increase of the SDF-1 expressions may be involved in the immunopathogenesis of RA.

신뢰성 해석을 이용한 교량구조물의 설계VA기법 연구

김성일,이광모,최석원,정준화,김성일,Kim, Seong-Il,Lee, Kwang-Mo,Choi, Suk-Won,Jung, Jun-Hwa,Kim, Seong-Il 한국건설관리학회 2009 한국건설관리학회 논문집 Vol.4 No.3

본 연구에서는 확률적 LCC 및 확률적 성능평가를 고려한 설계 VA분석 방법을 제안하였으며, 신뢰성 해석의 개념을 도입하여 신뢰도를 확보한 의사결정을 지원하고자 하였다. 상기와 같은 목적과 방법에 따라 진행된 본 연구의 결과를 요약하면 다음과 같다: 1) 기능정의가 완료된 이후 도출된 대안들의 대안평가시 신뢰성 있는 분석을 위하여 개선된 설계VA 절차 와 가치상태함수를 정식화하였으며, 가치평가절차에서의 일관성 및 효율성을 확보하기 위하여 평가지표방안을 제시하였다. 2) 교량의 LCC분석을 위한 DB를 수집 및 분석하였다. 자료의 수집범위는 기존에 수행된 교량의 LCC분석 연구 문헌을 기초로 하여 분석하였으며, 분석결과에 대한 신뢰도 확보 및 수집된 자료의 불확실성 처리를 위해 MCS 기법을 적용하였다. 3) 대안별 성능평가를 위한 가중치 및 평가등급과 LCC 분석을 위한 LCC 분석모델, 분석기간, 할인율, 사용자비용, 안전점검 및 안전진단비용에 대한 조건을 제시하였다. 끝으로, 사례대상인 "OO대교 및 연결도로 건설공사 실시설계" 프로젝트에 대한 VA수행 사례를 중심으로 타당성을 검토하고 결론을 도출하였다. In this study, a design value analysis technique that considered stochastic LCC and stochastic performance evaluation was proposed, and by introducing the concept of reliability analysis, a decision making that secured reliability was supported. The results of this study, which was carried out according to the above objectives and methods, are summarized as follows: 1) The design value analysis procedures and value state function, improved in order to carry out a reliable analysis when evaluating alternate proposals that were extracted after the function definition was complete, were formalized, and in order to secure consistency and efficiency for value evaluation procedures, an evaluation index scheme was proposed; 2) Database collection and analysis were done for a bridge's LCC analysis. As for the collection scope of data, literature of previous research done on a bridge's LCC analysis was used as the basis for analysis, and for securing reliability regarding analysis results and dealing with uncertainty of collected data, the MCS technique was applied; 3) Weights and evaluation ranks for performance evaluation of each of the alternate proposals, as well as LCC analysis model, analysis period, discount rate, user expense, safety inspection and safety diagnosis expense conditions for LCC analysis were proposed. Lastly, a feasibility study was done and conclusion was made about "OO grand bridge and connecting road construction work execution design" project centered on value analysis execution case.

여러 가지 높이를 갖는 삼각형 구조 InGaAs/GaAs 양자세선 구조 성장

김성일,김영환,한일기,Kim Seong-Il,Kim Young-Whan,Han Il-Ki 한국재료학회 2004 한국재료학회지 Vol.14 No.6

InGaAs/GaAs quantum wire structures were grown by low pressure metalorganic chemical vapor deposition by using selective area epitaxy.$ In_{ 0.2}$$Ga_{0.8}$ As/GaAs quantum wire structures were grown on a $SiO_2$ masked GaAs substrate. Quantum wire structures with sharp tips and smooth side walls were grown. We have grown InGaAs/GaAs quantum wire structures using variously opened width of the $SiO _2$ mask. Even though the opening widths of $SiO_2$ masked GaAs substrate were different, similar shapes of triangular structures were grown. Using various kinds of differently opened $SiO_2$ masked area, it would be possible to grow quantum wire structures with various thicknesses. The quantum wire structures are formed near the pinnacle of the triangular structure. Therefore, the fabrication of the uniquely designed integrated optical devices which include light emitting sources of multiple wavelength is possible.

근적외선 필터용 인산계 유리의 광학적 특성 및 구조적 특성에 미치는 CuO 의 영향

김성일,황종희,임태영,김진호,김영호,이종화,최덕균,Kim, Seong-Il,Hwang, Jong-Hee,Lim, Tae-Young,Kim, Jin-Ho,Kim, Young-Ho,Lee, Jong-Hwa,Choi, Deuk-Kyun 한국재료학회 2009 한국재료학회지 Vol.19 No.12

Optical characteristics and structural changes depending on CuO content in phosphate glasses that are used in near-infrared (near-IR) filters were investigated. With phosphate glasses that contain 1-9 mol% CuO, changes in optical transmittance, optical absorption, and color coordinate were measured with a UV-VIS spectrophotometer. An XPS (X-ray photoelectron spectroscopy) analysis was performed to determine valence of copper ion that influences optical characteristics in near-IR filter glasses. Structural changes in glasses depending on CuO content were also analyzed by FT-IR (Fourier transform infrared) and Raman spectrophotometers. From the UV-VIS spectrophotometer results, strong absorption peaks at 220 & 900 nm were found and transmittance was decreased. The color coordinates of the glasses were shifted to the green color direction with CuO addition for increasing absorption of long wavelength range spectra, in spite of the amount of $Cu^{2+}$, which gives a blue color to glasses, and which was increased in XPS results. Also, structural de-polymerization of glasses with CuO addition were found by FT-IR and Raman results.

특발성 염증성 근육병증 환자에서 IL-17 발현의 증가

백승훈 ( Seung Hoon Baek ),이준희 ( Jun Hee Lee ),김근태 ( Geun Tae Kim ),이정욱 ( Joung Wook Lee ),조미라 ( Mi Ra Cho ),김주인 ( Ju In Kim ),이선희 ( Sun Hee Lee ),김대성 ( Dae Seong Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2008 대한류마티스학회지 Vol.15 No.2

Objective: Idiopathic inflammatory myopathies (IIMs) are systemic autoimmune diseases characterized by infiltration of T lymphocytes, monocytes, and macrophages in muscle tissues. Interleukin-17 (IL-17), a Th17 cytokine, has potent pro-inflammatory actions and plays a role in autoimmune diseases. We investigated the expression of IL-17 in muscle tissues of patients with IIMs. Methods: We measured the IL-17 mRNA level of muscle tissues from 14 patients with IIMs (9 patients with dermatomyositis and 5 patients with polymyositis) by real-time RT-PCR and compared with controls. We also performed an immunohistochemical stain to detect IL-17 expression. Results: The expressions of IL-17 were significantly enhanced in IIMs than controls. In immunohistochemistry, IL-17 was expressed in perimysial, endomysial and perivascular infiltrating inflammatory cells. Conclusion: These results suggest that IL-17 plays a role in the immunopathogenesis of IIMs.

회원작품 - 블랑블루

김성일,Kim, Seong-Il 대한건축사협회 2016 建築士 Vol.2016 No.5

교수의 역할 수행은 노화에 따라 감소되는가?

김성일,Kim, Seong-Il 한국대학교육협의회 2007 大學敎育 Vol.146 No.-

태양전지 산업의 동향 - 집광형 태양전지 시스템 연구 및 개발동향

김성일,Kim, Seong-Il 한국광학기기산업협회 2009 光學世界 Vol.123 No.-

현재 전 세계적으로 한정된 천연 화석 연료의 고갈, 유가 상승과 지구 온난화 등의 영향으로 여러 가지 신재생 에너지, 그 중에서도 특히 태양전지를 이용한 에너지 재생 분야에 대한 관심이 매우 커지고 있다. 현재 연구 개발 및 상용화가 진행 중인 여러가지 태양전지의 효율 증가 추세를 살펴보면, III-V 화합물 반도체 태양전지가 효율이 가장 높고, 효율 증가 추세가 당분간은 계속적으로 지속될 것으로 예상된다. 따라서 크게 나누어 보면 III-V 태양전지는 고효율화를 지속적으로 추구하고, 기타 태양전지들은 저가화 방향으로 연구개발이 주로 진행될 것으로 예상된다.

인체이식형 압력센서 동향

김성일,박재순,정연호,Kim, Seong-Il,Park, Jae-Sun,Jeong, Yeon-Ho 한국전기전자재료학회 2015 전기전자재료 Vol.28 No.1

뼈 모사체에서 다공율 및 구조에 대한 음속 및 감쇠계수의 변화

김성일,최민주,이강일,Kim, Seong-Il,Choi, Min-Joo,Lee, Kang-Il 한국음향학회 2010 韓國音響學會誌 Vol.29 No.6

본 연구에서는 뼈에서 다공율 및 골미세구조에 대한 음속 및 감쇠계수의 변화를 살펴보기 위하여 원통형 다공을 갖는 폴리아세틸로 제작된 뼈 모사체가 이용되었다. 0 %부터 65.9 %까지의 다공율을 갖는 6개 뼈 모사체의 음속 및 감쇠계수는 12.7 mm의 직경 몇 1.0 MHz의 중심 주파수를 갖는 한 쌍의 광대역, 비집속형 초음파 트랜스듀서를 이용하여 수중에서 투과법에 의하여 측정되었다. 음속은 뼈 모사체의 구조적 특성에 상관없이 다공율이 증가함에 따라 거의 선형적으로 감소하였다. 1.0 MHz에서 측정된 감쇠계수는 뼈 모사체의 구조적 특성에 따라 다공율에 대하여 선형적인 또는 비선형적인 상관관계를 나타냈다. 이와 같은 결과는 뼈 샘플 및 뼈 모사체를 이용하여 다른 연구자들에 의하여 발표된 결과와 잘 일치하며, 사람의 뼈에서 골다공증 진단을 위한 초음파 변수와 골밀도 및 골미세구조 사이에 존재하는 상관관계를 이해하는데 도움이 된다. In the present study, polyacetal bone mimics with circular cylindrical pores were used to investigate variations of speed of sound and attenuation coefficient with porosity and microarchitecture in bone. The speed of sound and attenuation coefficient of the 6 bone mimics with porosities from 0 % to 65.9 % were measured by a through-transmission method in water, using a pair of broadband, unfocused transducers with a diameter of 12.7 mm and a center frequency of 1.0 MHz. Independently of the structural properties of the bone mimics, the speed of sound decreased almost linearly with the increasing porosity. The attenuation coefficient measured at 1.0 MHz exhibited linear or nonlinear correlations with the porosity, depending on the structural properties of the bone mimics. These results are consistent with those previously published by other researchers using bone samples and mimics, and advances our understanding of the relationships of the ultrasonic parameters for the diagnosis of osteoporosis with the bone density and microarchitecture in human bones.