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김배환 ( Bae Hwan Kim ),이준혁 ( Joon Hyuk Lee ),백두산 ( Du San Baik ),윤승욱 ( Seoung Wook Yun ),김지환 ( Ji Hwan Kim ),공재환 ( Jae Hwan Kong ),김석배 ( Seok Bae Kim ) 대한소화기학회 2007 대한소화기학회지 Vol.50 No.6
Gastrointestinal stromal tumor (GIST) is an uncommon mesenchymal tumor of the gastrointestinal tract and is generally located in the stomach and small intestine. They usually present with abdominal pain, gastrointestinal bleeding, and palpable mass. Some patients present with rare symptoms that are more common in malignant GIST. Malignant GIST combined with a liver abscess has not been reported yet in the literatures. We report a case of 67-year-old woman who suffered from liver abscess combined by malignant GIST with central necrosis and fistula in the ileum. She complained of fever, chills, and abdominal pain. Abdominal CT scan showed huge liver abscess and ileal mass with air pocket. Small bowel series showed contrast material filling into the ileal GIST mass. An operation was performed and the final diagnosis was malignant GIST of the ileum with invasion into the sigmoid colon and urinary bladder. (Korean J Gastroenterol 2007;50:393-397)
김배환 ( Bae Hwan Kim ),( Chae Wook Kim ),( Kyoung Mi Jung ),( Byung Fhy Suh ) 한국동물실험대체법학회 2008 한국동물실험대체법학회 학술대회집 Vol.2008 No.1
The hen`s egg test-chorioallantoic membrane (HET-CAM) assay, which employs the vascular membrane of a fertile hen`s egg, has been actively studied by several countries as an alternative method to the Draize rabbit eye irritation test. However, the scoring system of the original HET-CAM test is not satisfactory because of its lack of objectivity and quantitativeness. Moreover, the test required skill when the effects of test substance are to be regarded. To overcome these disadvantages, Hagino et al. developed and objective evaluation technique using the amount of trypan blue absorbed at the site of treatment as an indicator of injury to the CAM (Chorioallantoic membrane-trypan blue staining, CAM-TBS). In this study, we evaluate the CAM-TBS methods and used three materials (1%, 2%, 5% SLS, tween 20 and PBS) to evaluate the correlation of CAMTBS with HET-CAM test. The amounts of trypan blue adsorbed with the CAM showed a good correlation with the HET-CAM test score(r=0.958). According to these results, we suppose that the trypan blue staining assay using CAM-TBS may be a good method to replace the HET-CAM test as an alternative to Draize rabbit eye irritation test. Despite our results, additional studies with more materials are necessary to validate this method.
Hen`s egg test-chorioallantoic membrane(HET-CAM) assay를 이용한 Retinol 및 Kojic acid의 자극평가
김배환 ( Bae Hwan Kim ) 한국동물실험대체법학회 2012 동물실험대체법학회지 Vol.6 No.1
The hen`s egg test-chorioallantoic membrane(HET-CAM) assay, an alternative method to eye irritation test, employs the vascular membrane of a fertile hen`s egg. Retinol has been usually used for topical treatment, because it has various effects such as anti-wrinkle and skin disorders. Kojic acid has been also extensively used for topical treatment, because it is well known for its anti-pigmentation effects cause by tyrosinase inhibition activities. In this study, we conducted irritation test of retinol using HET-CAM assay to compare with the result of Draize rabbit eye irritation test.
Diallyl Sulfide 와 Diallyl Disulfide 에 의한 랫드의 간암발생 억제효과에 관한 연구
김배환(Bae Hwan Kim),이영순(Yong Soon Lee) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.4
This study examined the anti-cancer effects of diallyl sulfide(DAS) and/or diallyl disulfide(DDS), major components of garlic oil, with the DEN-PH model in rats, by the numbers and areas per ㎠ of induced glutathion S-transferase placental form(GST-P) positive foci and silver-stained nucleolar organizer regions(AgNORs) counts per nuclei in liver as indicator. Sprague-Dawley(SD) rats were given the diethylnitrosamine(DEN, 200 ㎎/㎏, i.pJ as initiator and 2 weeks later, in experiment I, rats were treated with DAS(200 ㎎/㎏, i.g.) and/or DDS(50 ㎎/㎏, i.g.) for 6 weeks, respectively and concomitantly and also were given the same dose of DAS and/or DDS prior to DEN treatment for 2 weeks, and in experiment II, rats were treated with potential cancer promoter, 2-acetylaminofluorene (2-AAF, 20 ㎎/㎏, i.g.). The DAS and/or DDS were treated prior to 2-AAF for 8 weeks, respectively and concomitantly. Then the anti-promoting effects of DAS and/or DDS were assessed. All rats were subjected to the two-thirds partial hepatectomy(PH) at week 3 and sacrificed at week 8. In experiment I, DAS and/or DDS treatment only prior to DEN showed inhibition of the development of GST-P positive foci. In experiment II, DAS and/or DDS treatment prior to 2-AAF promotion showed obvious inhibition of the development of GST-P positive foci in numbers and areas and AgNORs counts. In conclusion, We found DAS and/or DDS had the preventive effects on the hepatocarcinogenesis in rats and the concomitant treatment had some additive effects compared with the each treatment and AgNORs counts correlated well with the preneoplastic hepatic lesion.
In vivo에 의한 미색류 콘드로이틴황산의 기능성 화장품 소재로서의 가능성
김배환(Bae-Hwan Kim),안삼환(Sam-Hwan Ahn),최병대(Byeong-Dae Choi),강석중(Seok-Joong Kang),김영림(Yong-Lim Kim),이후장(Hu-Jang Lee),오명주(Myung-Joo Oh),정태성(Tae-Sung Jung) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.4
단회경구독성시험에서 미더덕 및 멍게 유래 crude 콘드로 이틴황산의 LD_(50)는 2 g/㎏ 이상으로서 판명되어, 단회경구 독성 측면에서는 안전한 원료로 판단되었다. 비록 기니픽을 이용한 감작성시험은 미더덕 유래 crude 콘드로이틴황산만을 사용하여 실시하였고, 시험 농도는 GAGs의 임상 사용 예정량이 0.03~0.15%임을 고려하여 inductiuon 농도는 임상사용 예정량의 10배인 3.0%, challenge 적용 농도를 0.3% 이하로 잠정 설정하여 3.0%, 0.3%, 0.03% 농도에서 실시하였다. 그 결과 0.03% 및 0.15% 농도에서는 반응을 나타낸 개체가 없었으나, 양성대조군과 비교할 때 0.3% 농도에서는 24시간 후에는 10% 그리고 48시간 후에는 5%의 반응율을 나타냈지만 사용가능범위에 해당되어 미더덕의 GAGs은 감작성 측면에서 안전성이 높은 원료로 0.3%이하 농도에서는 사용 가능하다고 판단되었다. 토끼를 이용한 안점막 자극시험에서는 미더덕과 멍게 유래 crude 콘드로이틴황산 및 bovine trachea 콘드로이틴황산의 3.0% 적용시 모두 A.O.I.가 기준치 이하로 나타나 실제 사용시 눈에 자극성이 없음이 판명되었다. 토끼를 이용한 피부일차자극 시험에서는 미더덕 및 멍게 유래 crude 콘드로이틴황산을 3.0% 및 1.5%는 무자극을 보였지만, 멍게 유래 crude 콘드로이틴황산에서는 미약하게 보이던 피부반응이 미더덕 유래 crude 콘드로이틴황산에서는 3.0% 적용에서 다소 강한 반응을 보였다. 광독성 및 광감작성을 위한 UV 흡광 실험결과 미더덕 및 멍게 유래 crude 콘드로이틴황산과 대조군으로서 사용한 표품 콘드로이틴황산 모두 자외선영역(280~420 ㎚)에서 흡광을 보이지 않아서 광독성 및 광감작성 시험을 시행하지 않았다. 실험동물을 사용하여 조사한 결과 화장품 원료로서의 미더덕 및 멍게 유래 crude 콘드로이틴황산은 3.0% 이내에서는 사용이 안전함이 증명되었고, 멍게가 미더덕 유래의 콘드로이틴황산에 비교하여 생체반응에 나은 것으로 나타닜다. Crude chondroitin sulfates extracted from midduck tunics (Styela clava) and munggae tunics (Halocynthia roretzi) were examined in vivo in order to be utilized as a cosmetic material which was followed by an in vitro assay. Examinations, such as acute oral toxicity, skin sensitization, acute eye irritation, and primary skin irritation, were performed with a variety of laboratory animals. Phototoxic and photosensitization tests were not conducted since all chondroitin sulfates failed to absorb U.V. light at the range of 280 to 420 nm. In acute dermal and eye irritation, both specific clinical signs and dead cases were not demonstrated during the test period, but crude chondroitin sulfates from midduck and munggae tunics, and standard chondroitin sulfate from bovine trachea were showed 2.5, 1 and 1.25 of acute ocular irritation index (A.O.I.), respectively. In the case of skin sensitization, crude chondroitin sulfate from midduck tunics exhibited neither specific clinical signs nor dead cases in the entire course of the examination. While in acute oral toxicity, crude chondroitin sulfates from both midduck and munggae tunics found neither specific clinical signs nor dead cases during the test, and LD50 was suspected to be over 2 g/㎏. Based on this study, it was proven that crude chondroitin sulfates from either midduck or munggae tunics can be used safely as a cosmetic material.