기계화 수확 적합 단간 찰수수 “소담찰”

김정인(Kim Jung-In),김성국(Kim Sung-Kook),정태욱(Jung Tae-Wook),곽도연(Kwak Do-Yeon),김기영(Kim Ki-Young),고지연(Ko Jee-Yeon),우관식(Woo Koan-Sik),송석보(Song Seok-Bo),오인석(Oh In-Seok),최명은(Choe Myeong-Eun) 한국육종학회 2017 한국육종학회지 Vol.49 No.1

The sorghum (Sorghum bicolor (L.) Moench) variety ‘Sodamchal’ was developed and registered by Department of Sothern Area Crop Science, National Institute of Crop Science (NICS) in 2013. This variety was developed from the cross between ‘Hwanggeumchal’ with brown grain and ’Jungmo4001’ with semi-dwarf trait. ‘Sodamchal’ is the first semi-dwarf stature with waxy endosperm ever developed in Korea. The yield performance of ‘Sodamchal’ was 2.95 MT/ha in local adaptability test for 2 years. It had 91 cm culm length, which was 58 cm shorter than that of ‘Hwanggeumchal’, 26.3 cm panicle length, 22.0 mm thickness of culm, 21.1 g in 1,000 grain weight. It could be reduce the lodging problem and also adapted to mechanized cultivation. ‘Sodamchal’ produces high quantities of polyphenol and radical scavenging activitiy with diphenyl-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in pigmented testa. Above all ‘Sodamchal’ is suitable for use as a parental source for the development of improved pollinator parent lines for developing semi-dwarf sorghum with waxy endosperm and antioxidant activity. (Variety registration No. 6244)

술후성 상악낭종 - 증례보고 및 문헌적고찰 -

김진(Jin Kim),차인호(In Ho Cha),이충국(Choong Kook Lee),양성익(Sung Ik Yang) 대한악안면성형재건외과학회 1986 Maxillofacial Plastic Reconstructive Surgery Vol.8 No.1

The postoperative maxillary cyst develops as a delayed complication many years after surgical intervention in the maxillary sinus. Clinically, the patient usually complains the swelling or pain of the cheek and the mucogingival fold of maxilla. Histologically, the cyst appears to be derived from the epithelial lining of the maxillary sinus which apparently has been trapped in the wound during closuse of the incision and subsequently begins to proliferate. We have treated 5 cases of postoperative maxillary cyst which were confirmed pathologically during recent 1 year. The results are as follows; 1. In case 1, we have found the independent 4 cystic cavity which were very rare. 2. In most cases, the chief complains were midfacial or buccal swelling. 3. The above all cysts were developed after Caldwell-Luc`s operation about 10-33 years ago. 4. distribution was in 2nd-5th decade. 5. In all cases, the patients complained the associated tooth symptoms. During the cyst treatment, we have proper tooth treatment together, the results of which were favorable.

우울증 생쥐 모델에서 반하후박탕가미(半夏厚朴湯加味)의 항우울 효과

김국기 ( Kook Ki Kim ),이상룡 ( Sang Ryong Lee ),정인철 ( In Chul Jung ) 대한한방신경정신과학회 2014 동의신경정신과학회지 Vol.25 No.2

Objectives: The purpose of this study was to examine the anti-depressive effects of BanHaHuBakTang-kami (BHHBT) on an animal model of depression induced by chronic immobility stress. Methods: Mice were treated daily with immobilization stress for 2 hours over a period of 21 days. To examine the effect of BHHBT, we performed behavioral, biochemical and histological analysis to measure immobility time (FST), brain neurotransmitter concentration (HPLC, ELISA), hippocampal damage (H&E staining) and CRF-R1 expression (immunohistochemistry). Results: BHHBT has reduced the immobility time of immobilization stress exposed mice in the forced swimming test. BHHBT has increased the amount of serotonin in the brain. BHHBT has increased the expression level of serotonin in the brain. BHHBT 540 mg/kg were sufficient to prevent tissue damage in the hippocampus region. BHHBT has reduced the expression level of CRF receptors in the hippocampus region. Conclusions: These results suggest that BHHBT may have anti-depressive effects on mice treated with immobilization stress by reducing immobility, increasing brain serotonin concentration and reducing CRF-R1 expression in the hippocampus region.

연소화염이 플래시오버 특성에 미치는 영향

김인식(In-Sik Kim),김이국(Lee-Kook Kim),박재용(Jai-Yong Bak),이상우(Sang-Woo Lee),김충년(Chung-Nyun Kim),지승욱(Seung-Wook Jee) 한국조명·전기설비학회 2001 한국조명·전기설비학회 학술대회논문집 Vol.2001 No.-

In this paper, reduction characteristics of the ac flashover voltage in the horizontal air gap of sphere-sphere and needle-needle electrode system were investigated when the combustion flame was present near the high-voltage electrodes. The reduction characteristics of ac flashover voltage were discussed with the thermal ionization process, the relative air density and the deflection phenomena in the shape of flames that changed by the corona wind and coulomb‘s force. As the results of an experimental, it was found that the reduction of flashover voltages in sphere-sphere system, in comparison with the no-flame case, are 79.9[%] for k=0, 82.9[%] for k=0.5, 87.5[%] for k=1.0, 85.0[%] for h=0[cm], 40.8[%] for h=5[cm] and 28.2[%] for h=9[cm] when ac voltage is applied.

생쥐 1-세포기배의 초급속 동결에 있어서 평형 온도와 노출시간의 영향

정덕수,김형국,박인국,Chung, Duk-Soo,Kim, Hyung-Kuk,Park, In-Kook 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to playa key role in the development of the embryo. In general, the development of the embryo was more effective at $3^{\circ}C$ than $23^{\circ}C$ and 4.5 M than 3 M for 3 to 5 minutes. At $23^{\circ}C$ the development of the embryo was stimulated by DMSO while at $3^{\circ}C$ it was stimulated by PROH. Thus it has been suggested that there exists a correlation between the concentration of cryoprotectants and exposure time in the development of the embryo. In conclusion, we found that for ultrarapid freezing of mouse 1-cell embryos in DMSO, or PROH-based solution, viability shown optimum depending on the cryoprotectant, the concentration of the cryoprotectant and on the temperature and the duration of equilibration.

한국인의 유전성 혈전증

김인호(In Ho Kim),박선양(Seon Yang Park),이종태(Jong Tai Lee),방수미(Soo Mee Bang),김효수(Hyo Su Kim),김병국(Byoung Kook Kim),김노경(Noe Kyeong Kim),박성섭(Seong Sub Park),조한익(Han Ik Cho),정해영(Hae Young Jeong),유욱준(Ook Joon Yo 대한내과학회 1996 대한내과학회지 Vol.51 No.6

N/A Objectives: Thromboembolism is a serious medical problem causing considerable morbidity and mortality. Major clinical risk factors for thrombosis included surgery, fracture, malignancy, old age, immobilization and the use of oral contraceptives. In the last several decades, substantial progress has been made in identifying hereditary factors predisposing to thrombosis. The genetic defects known to be associated with thrombophilia are deficiencies of antithrombin 3, protein C, protein S, dysfibrinogenemia and resistance to the anticoagulant action of activated protein C. We have elucidated the characteristics of heriditary thrombophilia of the Korean patients. Methods: The clinical profiles of 48 patients with heriditary thrombophilia (12 cases of our hospital and 36 cases reported previously in Korea) were analyzed. The underlying hemostatic abnormalities about antithrombin 3, Protein C, Protein S, activated protein C, fibriongen were investigated. Family studies of 6 patients of our 12 patients were done. Nucleotide sequences of antithrombin 3 genes of 2 patients were studied. Results: 1) Seven patients (58%) among our 12 patients had thrombotic onset before fifth decades, and 5 patients developed thromboses at their third decades. 2) Pulmonary embolisms were diagnosed in 10 cases (83%) among our 12 cases. Deep vein thromboses of lower extremities were 8 cases (67%) and usually rare site thromboses like a portal vein thrombosis occurred frequently (8 cases, 67%). Arterial thromboses occurred in two cases (17%). Hereditability of 5 families were confirmed by family study. 3) Analysis of 48 cases showed that protein C deficiency (12 cases, 25%) and protein S deficiency (19 cases, 40%) occurred relatively frequently. 4) Neither Resistance to activated protein C nor mutation in the factor V gene was demonstrated in Korean patients with deep vein thrombosis and normal persons. This result suggests that activated protein C resistance may be extremely rare in Korean population. 5) Two new mutations of antithrombin 3 genes were identified in two patients via nucleotide sequencing, and they were named 'AT 3 Seoul' and 'AT 3 Kosung' respectively. Conclusion: We elucidated clinical and laboratory characteristics of hereditary thrombophilia in Korea. Hereditary thrombophilia were not uncommon in Korean patients with deep vein thrombosis, with/without pulmonary embolism. Strong suspicion for hereditary thrombophilia may lead to correct diagnosis and appropriate treatment in these patients.

울산광역시 일개 초등학교 소아들의 알레르기 및 아토피질환의 유병률

윤재국 ( Jae Kook Yoon ),심창선 ( Chang Sun Sim ),최승원 ( Seung Won Choi ),오인보 ( In Bo Oh ),이지호 ( Ji Ho Lee ),김양호 ( Yang Ho Kim ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.2

Background: Ulsan is the most industrialized city in Korea. Although many investigations as to which factors are related to allergy and atopy have been performed so far, these factors are still uncertain. Studies of industrialized environment can help to understand an atopic disorder. Objective: This study was undertaken to investigate the prevalence of allergic and atopic disorders in an industrial city, Ulsan and to estimate the proportion of ``real`` atopyto so-called ``allergy and atopy``. Also, we examined the validity of some items of blood tests, such as serum total IgE levels and eosinophil counts for the diagnosis of the atopic disorder. Method: We conducted the questionnaire survey based on the ISAAC protocol along with demographic variables at an elementary school in the central area of Ulsan. The questionnaires were completed by patients of 1,323 children, of whom 570 participated in skin prick test and blood test. Atopic disorder was defined as the presence of allergic symptoms and the presence of sensitization to any allergens by skin prick test. Result: The lifetime prevalence of atopic wheeze was 8.8%and the proportion of atopy of asthma was 60.4%. The lifetime symptom prevalence of ``real`` atopic dermatitis (atopiceczema) was 16.1% and the proportion of atopy of atopic dermatitis was 49.4%. The prevalence of ``real`` allergic rhinitis (atopic rhinitis) was 28.0% and the proportion of atopy of allergic rhinitis was 51.8%. The prevalence of overall atopic disorder was 37.0%, and the proportion of atopy of overall allergic disorder was 51.4%. The area under receiver operating characteristic curve of serum total IgE for the diagnosis of the atopic disorder was 0.770. Also, we observed the selective bias from the differences in prevalence between groups of complete participants and incomplete participants. Conclusion: The prevalence of asthma was similar to those of other regions in Korea. However, atopic dermatitis and allergic rhinitis were more prevalent than other regions. About half of so-called allergic disorders may be an atopic disorder. (Korean J Asthma Allergy Clin Immunol 2011;31:105-115)

고프롤린식이에 의한 백서 소장에서의 ACE과 DAP-IV 유도의 유전적 조절기전에 대한 연구

이동호(Dong Ho Lee),김재준(Jae Jun Kim),임선희(Sun Hee Lim),윤병철(Byung Chul Yoon),이국래(Kook Lae Lee),정현채(Hyun Chae Jung),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.3

N/A Background/Aims: Angiotensin converting enzyme(ACE) and Dipeptidyl aminopeptidase IV(DAP- IV) are well known brush border rnembrane enzymes which have major role in digestion of prolyl peptide(proline). Even though high proline diet is known to induce the increase of enzymatic activity of ACE and DAP-IV, the genetic mechanism of this phenomenon has not been recognized. Therefore we performed this study to clarify the genetic mechanism of ACE and DAP-IV induction by high proline diet. Methods: Total 20 Wistar rats were divided into four groups with different diets. Control group was fed with normal diet(17% protein), sucrose group was fed with high sucrose diet(68% sucrose, 18% protein), protein group was fed with high protein diet(50% casein), and gelatin group was fed with high proline diet(50% gelatin). After 3 weeks the small intestine was removed and divided into three equal length segments, from which homogenate, btesh border membrane and total RNA were prepared. ACE and DAP-IV enzyme activity were estimated in both small intestine homogenate and brush border membrane. The amounts of ACE mRNA and DAP-IV mRNA were also estimated by Northern analysis of RNA. Results: ACE activity of homgenate: There was significant increase of ACE activity in gelatin group(pC0.01) and protein group(p(0.05) throughout all sites(proximal, middle, distal). ACE activity of brush border membrane: There was significant increase of ACE activity in distal site(p0.01) of gelatin group and in middle(p0.05) and distal site(p0.05) of protein group. DAP-IV activity of homogenate: There was significant increase of DAP-IV activity in middle(p<0.01) and distal(p(0.05) site of gelatin group. DAP-IV activity of brush border membrane: There was significant increase of DAP-IV activity in gelatin group(p(0.01) and protein group(pC0.01) throughout all sites. ACE mRNA amount: Gelatin group showed increase of ACE mRNA in proximal site(p C0.05) and total mean value(p0.05). Protein group also showed increase of ACE rnRNA in proximal(pC0.05)), middle(pa0.05) and total mean value(p0.05). DAP-IV mRNA amount: There was no statistically significant change in gelatin and protein group. Conclusions: 1) High proline diet induced the increase of enzymatic activity of ACE and DAP-IV. 2) Increase of ACE activity was associated with increase of ACE mRNA in small intestine. 3) Increase of DAP-IV activity was not correlated with DAP-IV mRNA. These results suggested that the induction of increased ACE activity by high proline diet is regulated on transcriptional level and the induction of increased DAP-IV activity may be regualted on posttranscriptional level (translational and/or posttranslational level). In conclusion, the induction of increased enzyme activity of brush border membrane by specific diet may be regulated on both transcriptional level and posttranscriptional level. (Korean J Gastroenterol 1996; 28:349 - 361)

소아의 하악골을 침범한 랑거한스세포 조직구 증식증의 임상적 고찰

강연희,박세현,서동준,차인호,이충국,김현실,김진,김형준,Kang, Yeon-Hee,Park, Se-Hyun,Seo, Dong-Jun,Cha, In-Ho,Yi, Choong-Kook,Kim, Hyun-Sil,Kim, Jin,Kim, Hyung-Jun 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.6

Langerhans cell histiocytosis (LCH) is characterized by proliferation of pathological Langerhans cells within different organs. It mainly affects children, but adult cases also occur, with an incidence rate of one to two per million. LCH results from the clonal proliferation of Langerhans cells. And its etiopathogenesis is still unknown. The hypothesis that it is a neoplastic or inflammatory disease, as well as the existence or not of immunological, viral or genetic predisposing factors, has been widely discussed in the literature, but no conclusive proof has ever been provided. Although lesions may appear in tissues of various origins such as skin, hypothalamus, liver, lung, or lymphoid tissue, bone is the most common site of the disease. The head and neck are affected in almost 90% of cases. The maxillary and mandibular bones are affected in 5 to 10% of cases. In our report, we present four cases of LCH in patients aged 3, 4, 7 and 9 years respectively, with primary manifestation in maxillofacial area.

Modification of Endothelium on Contractile Response of Brain Vessels to Contracting Agents

국영종,백영홍,김종근,최봉규,최수형,김연인,Kook, Young-Johng,Baik, Yung-Hong,Kim, Jong-Keun,Choi, Bong-Kyu,Choi, Soo-Hyung,Kim, Yung-In The Korean Society of Pharmacology 1988 대한약리학잡지 Vol.24 No.2

돼지, 가표 및 가토의 뇌동맥에서 기저동맥 (basilar artery, BA)과 circle of willis동맥 (WC)의 동맥환을 만들어 혈관수축제와 calcium 길항제의 효과 그리고 내피세포의 역할은 검토하였다. 돼지 BA와 WC에 서 norepinephrine (NE), phenylephrine (PE)및 epinephrine (EP)은 propranolol $10^{-6}M$ 전처리하에서 그리고 KCI, histamine, 5-hydroxytryptamine (5-HT) 및 angiotensin은 모두 용량의존성 수축반응을 일으켰다. 그 최대수축력은 angiotensin에서 가장 적었고, 5-HT에서 가장 강력하였다. KCI 35 mM수축반응은 calcium 길항제로 용량의존성으로 억제되었고, 그 효력은 nifedipine > > diltiazem > flunarizine > oxybutynin > isosorbide dinitrate (ISDN) > glycerl trinitrate의 순서였다. 5-HT $10^{-5}M$의 수축반응은 nifedipine으로는 용량의존성으로 억제 되었으나 diltiazem과 ISDN으로는 경미한 억제만을 일으켰다. 내피세포동맥환에서 KCI 35 mM의 수축반응은 acetylcholine (ACh)으로 거의 영향받지 않았으나 $PGF_{2{\alpha}}\;10^{-5}M$의 수축반응은 ACh과 adenosine으로 용량의존성으로 억제되었고, 이 내피세포 의존성억제는 nifedipine $10^{-6}M$로는 영향받지 않으나 methylene blue $50\;{\mu}M$로는 현저히 억제되었다. 네피제거동맥환에의 $PGF_{2{\alpha}}$의 수축반응은 ACh의 영향이 없거나 더욱 수축되었다. 내피세포를 제거하지 않은 가토흉부대동맥편을 bath에 함께 넣어주면 ACh의 용량의존성 이완반응이 나타났고 이 이완반응도methylene blue로 억제되었다. 가묘 BA와 WC에서 5-HT와 NE는 용량의존성 수축반응을 일으켰고 ACh도 내피세포 존재유무와 관계없이 강력한 용량의존성 수축반응을 일으켰으며 그 최대수축력은 ACh에서 가장 강력하였다. 내피세포동맥환에서 5-HT $10^{-5}M$의 수축반응은 ACh에 의하여 이완반응없이 더욱 수측되었다. 가토 BA에서 5-HT와 NE는 응량의존성 수축반응을 일으졌고5-HT의 수축반응이 더욱 현저하였다. 내피세포동맥환에서 5-HT $10^{-5}M$의 수축반응은 ACh $10^{-5}M$로 현저하게 이완되있고 이 내피세포의존성 이완반응은 atropine $10^{-7}M$로는 억제되었으나 diltiazem $10^{-6}M$로는 거의 억제되지 않았다. 이상의 실험성적은 돼지와 가토의 뇌동맥에서 ACh은 내피세포-의존성 이완반응을 일으키며그 이완반응은 muscarinic receptor를 통해 나타났고, 가묘뇌동맥에서 ACh은 혈관수축제의 역할을 갖고 있음을 시사하고 있다. To delineate the mechanisms of vasoconstriction and vasodilation in cerebral arteries the effects of some vasoconstrictors and calcium antagonists on the basilar artery (BA) and arterial circle of Willis (WC) were examined and also the role of endothelium in the action of these drugs was investigated in pigs, cats and rabbits. In pig cerebral arteries, dose-dependent contractile responses were elicited by KCI, histamine, 5-hydroxytryptamine (5-HT) and angiotensin, but norepinephrine (NE), phenylephrine (PE) and epinephrine (EP) elicited dose-dependent contractions only under pretreatment with propranolol 10-6 M. The magnitudes of maximal contractile effects of these drugs were different from each other, and 5-H~ was the largest and angiotensin the smallest. Some calcium antagonists dose-dependently inhibited KCI (35 mM)-induced contraction and the order of potency in inhibiting the contraction was nifedipine > > diltiazem > flunarizine > oxybutynin > isosorbide dinitrate (ISDN) > glyceryl trinitrate. 5-HT (10-6 M)-induced contraction was dosedependently inhibited by nifedipine but slightly inhibited by diltiazem and ISDN. In rings with intact endothelium, KCI (35 mM)-induced contraction was not affected by acetylcholine (ACh) but $PGF_{2{\alpha}}$ (lO-SM)-induced contraction was dose-dependently relaxed by ACh and adenosine. This endothelium-dependent relaxation was not affected by nifedipine (l0-6M)-pretreatment but markedly inhibited by methylene blue (50,uM)-pretreatment. In the porcine arterial rings without endothelium, ACh had no effect or even contracted the $PGF_{2{\alpha}}-induced$ contraction. However, the dosedependent relaxing effect of ACh appeared when the deendothelized porcine ring and rabbit thoracic aorta with intact endotheli urn were simultaneously suspended into a bath and this relaxing effect was also inhibited by methylene blue-pretreatment. In cat cerebral arteries, 5-HT and NE elicited dose-dependent contractile responses and ACh also produced dose-dependent contraction regardless of the existence of endothelium. ACh-induced contraction was most prominent. 5-HT (IO-SM)induced contraction was not relaxed but contracted additionally by ACh even in the intact endothelial ring. In rabbit cerebral arteries, 5-HT and NE elicited dose-dependent contractile responses and 5-HT-induced contraction was more prominent. In the intact endothelial preparations, 5-HT (lO-s M)-induced contraction was markedly relaxed by the addition of ACh( IO-SM) and this endothelium-dependent relaxing effect was inhibited by atropine (l0-7M)-pretreatment but notaffected by diltiazem (l0-6M)-pretreatment. These results suggest that ACh elicits endotheliumdependent relaxing effect mediated by muscarinic receptors in cerebral arteries of pig and rabbit, and that ACh acts as vasoconstrictor in cat cerebral artery.