갑상선 유두암 세포주 TPC-1에서 Cyclooxygenase-2 억제제 NS-398의 증식억제효과

김광필,박진우,윤효영,장이찬,최재운 대한갑상선-내분비외과학회 2003 The Koreran journal of Endocrine Surgery Vol.3 No.2

Purpose: Cyclooxygenase (COX) enzymes catalyze the rate- limiting step in arachidonate metabolism. COX-1 is expressed constitutively in many cell types. However COX-2 is an inducible enzyme responsible for prostaglandin production at site of inflammation. Recently, there has been increasing evidence that COX-2 involves in development and progression of human tumors. The aim of the present investigation is to evaluate the antiproliferative effect of NS-398, a selective COX-2 inhibitor, and its mechanism in a papillary thyroid cancer cell line, TPC-1. Methods: We used TPC-1 cell line, NS-398 and EGF. COX-2 expression was detected by RT-PCR and western blot. We used MTT assay to evaluate antiproliferative effect of NS- 398. The mechanisms of growth inhibition were evaluated by apoptosis assay and cell cycle analysis using flow cytometry. Results: COX-2 expression was identified by both RT-PCR and western blot in TPC-1 cells and it was upregulated by serum, EGF (10 ng/ml), and NS-398 (50 mM). NS-398 induced a dose-dependent inhibition of cell proliferation but did not increases apoptotic cell population significantly in the TPC-1 cell line. EGF treatment (10 ng/ml) for 72 hours did not seem to change the antiproliferative effect of NS-398. The proportion of G0/G1 cell cycle was increased by 10% compared with control after 36 hours of treatment with NS-398. Conclusion: TPC-1 cells expressed COX-2 constitutively and its expression was upregulated by serum, EGF, and NS- 398. The selective COX-2 inhibitor, NS-398 inhibited cell proliferation in TPC-1 cell line rather by cell cycle arrest at G0/G1 phase than by inducing apoptosis. (Korean J Endocrine Surg 2003;3:106-112)

불안정한 설비중심공정의 Cycle Time 설정 연구

김광필,문기주 東亞大學校附設 生産技術硏究所 2007 生産技術硏究所硏究論文集 Vol.12 No.-

A possible approach to calculate and determine a cycle time for unstable facility based steelmaking process. Necessary analysis are done by measuring and analysing the process in detail to calculate standard time and process improvements. Between 4 and 8% of productivity improvements are expected to make based upon the collected manufacturing data.

수정된 이원평가표를 이용한 품질속성의 분류에 관한 연구

김광필,송해근 대한안전경영과학회 2018 대한안전경영과학회지 Vol.20 No.1

For several decades, attribute classification methods using the asymmetrical relationship between an attribute performance and the satisfaction of that attribute have been explored by numerous researchers. In particular, the Kano model, which classifies quality attributes into 5 elements using simple questionnaire and two-dimensional evaluation table, has gained popularity: Attractive, One-dimensional, Must-be, Indifferent, and Reverse quality. As Kano's model is well accepted, many literatures have introduced categorization methods using the Kano's evaluation table at attribute level. However, they applied different terminologies and classification criteria and this causes confusion and misunderstanding. Therefore, a criterion for quality classification at attribute level is necessary. This study is aimed to suggest a new attribute classification method that sub-categorizes quality attributes using 5-point ordinal point and Kano's two-dimensional evaluation table through an extensive literature review. For this, the current study examines the intrinsic and extrinsic problems of the well-recognized Kano model that have been used for measuring customer satisfaction of products and services. For empirical study, the author conducted a comparative study between the results of Kano's model and the proposed method for an e-learning case (33 attributes). Results show that the proposed method is better in terms of ease of use and understanding of kano's results and this result will contribute to the further development of the attractive quality theory that enables to understand both the customers explicit and implicit needs.

북부CT검사 시 Organ Dose 계산을 위한 CTDIvol 당 Conversion Factor

김광필(Kwang Pil Kim),김문찬(Moon Chan Kim),남윤철(Yoon Chul Nam) 대한CT영상기술학회 2012 대한CT영상기술학회지 Vol.14 No.2

목적 Monte Carlo simulation software tool을 기반으로 하여 CTDIvol과 organ dose와의 상관관계를 알아보고자 하였으며, 이를 바탕으로 organ dose를 쉽게 계산할 수 있는 CTDIvol 당 conversion factor를 제시하고자 하였다. 대상 및 방법 ImPACT에서 제시하는 Monte Carlo simulation program인 Patient Dosimetry Calculator(version. 0.99x)을 사용하였다. 복부CT를 대상으로 하였으며 인체모형의 수학적 팬텀의 liver, stomach, kidney, colon, bladder, gonads의 장기에 대한 CTDIvol 당 conversion factor를 구하였다. CT장치는 GE, Philips, Toshiba, Siemens 사의 4 MDCT, 16 MDCT, 64 MDCT를 사용하였으며, 각각의 장치에서 관전압, mAs, beam collimation, pitch factor를 변화시키며 제시된 organ dose를 CTDIvol으로 나누어 conversion factor를 계산하였다. 결과 • G사의 80kVp를 사용한 복부검사에서 4 MDCT 장치에서의 liver, colon, bladder, gonads의 conversion factor는 1.08, 0.56, 0.48, 1.01이었고, 16 MDCT에서는 1.21, 0.57, 0.40, 0.90, 64 MDCT에서는 1.22, 0.64, 0.46, 1.03이었다. 120kVp를 사용하면 4 MDCT에서 1.30, 0.68, 0.48, 1.11이었고, 16 MDCT에서는 1.28, 0.69, 0.48, 1.12, 64 MDCT에서는 1.23, 0.68, 0.48 1.09이었다. • P사의 90kVp를 사용한 복부검사에서 4 MDCT 장치에서의 liver, colon, bladder, gonads의 conversion factor는 1.26, 0.68, 0.47, 1.10이었고, 16 MDCT와 64 MDCT에서는 1.25, 0.66, 0.45, 1.07이었다. 120kVp를 사용하면 4 MDCT에서 1.28, 0.68, 0.47, 1.00이었고 16 MDCT와 64 MDCT에서는 1.36, 0.73, 0.50, 1.15이었다. • T사의 80kVp를 사용한 복부검사에서 4 MDCT 장치에서의 liver, colon, bladder, gonads의 conversion factor는 1.00, 0.51, 0.46, 0.96이었고, 16 MDCT와 64 MDCT에서는 0.86, 0.45, 0.40, 0.84이었다. 120kVp를 사용하면 4 MDCT에서 1.22, 0.61, 0.40, 0.92이었고, 16 MDCT와 64 MDCT에서는 1.24, 0.65, 0.46, 1.03이었다. • S사의 80kVp를 사용한 복부검사에서 4 MDCT 장치에서의 liver, colon, bladder, gonads의 conversion factor는 1.21, 0.64, 0.44, 1.04이었고, 16 MDCT에서는 1.19, 0.63, 0.43, 1.02, 64 MDCT에서는 1.36, 0.73, 0.52, 1.14이었다. 120kVp를 사용하면 4 MDCT는 1.24, 0.67, 0.46, 1.04이었고, 16 MDCT에서는 1.24, 0.66, 0.45, 1.04, 64 MDCT에서는 1.35, 0.73, 0.51, 1.16이었다. 결론 CT검사 시 장비콘솔에 표시되는 CTDIvol과 본 연구를 통하여 제시된 conversion factor를 사용하면 organ dose를 특별한 선량측정 장비 없이도 쉽게 계산할 수 있으며 환자의 피폭선량을 관리하는데 유용하리라 사료된다. I. Purpose To know the connection of CTDIvol and organ dose based Monte carlo simulation software tool, the foundation of that, to suggest conversion factor per CTDIvol that easily calculating organ dose. II. Meterial and Methods We used Patient Dosimetry Calculator(version 0.99x) which is Monte Carlo simulation program suggested ImPACT. The target is abdominal CT exam, to get conversion factor per CTDIvol for liver, stomach, kidney, colon, bladder, gonads organs of human body mathematical models. CT machines are used 4 MDCT, 16 MDCT and 64 MDCT by GE, Philips, Toshiba, Siemens, and to calculate conversion factor that organ dose, which are presented by changing kVp, mAs, beam collimation, pitch factor of each machine, divide by CTDIvol. III. Result • Abdomen-pelvis exam by G company 80 kVp: for 4 MDCT equipment, conversion factors of liver, colon, bladder, gonads are 1.08. 0.56, 0.48 and 1.01, for 16 MDCT, 1.21, 0.57, 0.40 and 0.90, for 64 MDCT, 1.22, 0.64, 0.46, and 1.03. For using 120kVp, 4 MDCT get 1.30, 0.68, 0.48, 1.11, 16 MDCT get 1.28, 0.69, 0.48, 1.12 and 64 MDCT get 1.23, 0.68, 0.48 1.09. • Abdomen-pelvis exam by P company 90 kVp: for 4 MDCT equipment, conversion factors of liver, colon, bladder, gonads are 1.26, 0.68, 0.47 and 1.10, for 16 MDCT and also 64 MDCT, 1.25, 0.66, 0.45 and 1.07. For using 120 kVp, 4 MDCT get 1.28, 0.68, 0.47, 1.08, 16 MDCT and 64 MDCT get 1.36, 0.73, 0.50, and 1.15 as the same. • Abdomen-pelvis exam by T company 80 kVp: for 4 MDCT equipment, conversion factors of liver, colon, bladder, gonads are 1.00, 0.51, 0.46 and 0.96, for 16 MDCT and also 64 MDCT, 0.86, 0.45, 0.40 and 0.84. For using 120 kVp, 4 MDCT get 1.22, 0.61, 0.40, 0.92, 16 MDCT and 64 MDCT get 1.24, 0.65, 0.46, and 1.03 as the same. • Abdomen-pelvis exam by S company 80 kVp: for 4 MDCT equipment, conversion factors of liver, colon, bladder, gonads are 1.21, 0.64, 0.44 and 1.04, for 16 MDCT, 1.19, 0.63, 0.43 and 1.02, for 64 MDCT, 1.36, 0.73, 0.52 and 1.14. For using 120 kVp, 4 MDCT get 1.24, o.67, o.46, 1.04, 16 MDCT get 1.24, 0.66, 0.45, 1.04 and 64 MDCT get 1.35, 0.73, 0.51, and 1.16. IV. Conclusions We consider that to calculate organ dose easily wing conversation factor through this study and CTDIvol presented on. equipment consol when doing CT exam without special dose measurement machines.

단일 입출고장치를 가진 다중 캐로절시스템의 운영방식에 관한 연구

김광필 ( Kwang Pil Kim ),강현순 ( Hyun Soon Kang ),김채수 ( Chae Soo Kim ) 대한설비관리학회 2009 대한설비관리학회지 Vol.14 No.2

Carousels are rotatable closed-loop storage systems for small items. We consider the multi-carousel system in which a S/R machine serves more than one carousel through a transfer device called traverser. The S/R machine is placed on the traverser and can be transferred to adjacent carousels. Since the cost of a traverser is estimated approximately to be a half of that of a S/R machine, the utilization of multi-carousel system is an effective way in reducing initial installation cost for a great number of manufacturing firms if the pallet demands are relatively low. In order to estimate the cost and performance accurately, analytic models of expected cycle time for single command and dual command operations. performance evaluations are fulfilled based on the expected cycle time models for each command operations.

컨테이너 보안장치 개발에서의 TRIZ 활용에 관한 연구

김광필(Kwang-Phil Kim),최형림(Hyung-Rim Choi),김재중(Jae-Joong Kim),이재기(Jae-Kee Lee),이강배(Kang-Bae Lee),김채수(Chae-Soo Kim) 한국정보기술학회 2013 한국정보기술학회논문지 Vol.11 No.7

Container security device(CSD), ConTracer is used as an equipment for container cargo transportation safety. It is mounted inside of the container to sense opening and closing incidents of container door and monitor temperature, humidity, shock and internal condition of containers. It Supports 2.4GHz and 433MHz radio frequency and local area network communication modules. As it should be compatible with CSD regulations of US DHS(Department of Home Security), there are some difficulties in satisfying the requirements. This paper introduces an empirical case study for development of CSD, which is named as ConTracer. We adopt TRIZ (6 Step Creativity) which is well known as an useful and inventive problem solving technique for contradictive probelm. By evaluation of the performance, we successfully acquired the appropriate solution for the problem.

항만 컨테이너터미널에서의 6시그마경영 도입에 관한 연구

김광필 ( Kwang Pil Kim ),김채수 ( Chae Soo Kim ) 대한설비관리학회 2010 대한설비관리학회지 Vol.15 No.1

Six-Sigma has been the most influential management innovation program since Motorola first invented in 1987 and its application has extended to non-manufacturing processes such as transactional process and R&D process. Some container terminals in Korea, they try to introduce and implement Six-Sigma projects to improve the efficiency of terminal operation. There are some differences in Six-Sigma implementation between manufacturing process and non-manufacturing process. In this paper, we propose an implementation procedure and framework for Six-Sigma project, which are suitable for container terminal in harbor. This paper also presents priority in Six-Sigma topics for container terminal, that is confirmed from a BB(black-belt) workshop outcome. We testified the efficiency of the proposed implementation procedure through an empirical case study for Six-Sigma projects in a container terminal.

Managing quality attributes using customer satisfaction coefficient

송해근,김광필,Song, Hae-geun,Kim, Gwang-pil Korea Safety ManagementScience 2017 대한안전경영과학회지 Vol.4 No.3

The two-way quality theory has been widely used as a method for classifying quality attributes for several decades. In particular, the Kano model that classifies attributes into not just conventional one-dimensional but must-be and attractive has gained popularity due to its applicability and ease of use. However, the wordings of the five alternatives in the Kano's questionnaire has been criticised for unclear meanings. This study proposes a new two-way model to classify attributes using 5-point Likert scale alternatives. For this, the current paper investigated a case of TV sets to examine how the proposed model works in comparison with the Kano model. The application results of the proposed model are different from the original one. The two-way model classifies quality attributes in more detail such as the "one-dimensional with an attractive tendency" attribute, which has a greater influence on satisfaction than dissatisfaction, the opposite "one-dimensional with a must-be tendency" attribute, and "highly one-dimensional" and "less one-dimensional" attributes. In this study, a potential satisfaction coefficient (PSC), a potential dissatisfaction coefficient (PDC), and an average potential coefficient (APC) to manage quality attributes are proposed and discussed for their utilization.

궤도차량 변속기 구동용 베벨기어의 개선설계

정재웅,김광필,지현철,문태상 한국기계가공학회 2015 한국기계가공학회지 Vol.14 No.2

Transmission of a tracked vehicle designed for multiple functions such as steering, gear-shifting, and braking is a core component of heavy vehicle to which the power is transferred based on combined technology of various gears, bearing, and fluid machineries. Robustness and durability of transmission, however, have been issued due to a large number of driving units and sub-components inside its body. The bevel gears are major components for the transmission of power in a transmission. Increasing the tooth surface roughness and chamfering of the bevel gears, especially, we aim to improve the quality of transmission. In this study, design structural evaluation is conducted on bevel gears of transmission for tracked vehicle using the ROMAX-DESIGNER program. By doing so, design safety of the bevel gears has been evaluated based on the gear strength theory of ANSI/AGMA 2003 B97 standard.

수요변동시 공동영역을 도입한 자동창고의 운영방안

황규성,김광필 동의공업대학 2001 論文集 Vol.27 No.1

Performances of common zone with various sizes are examined for possible demand rate variations for an AS/RS. Common zone is a middle area located between the 1st class and the 2nd class to be used by the 1st class items if the assigned racks are not enough. This area is designed to resolve the rack shortage problem associated with a particular class. In the previous researches, effect of demand rate variation is ignored since fixed demand rate is assumed. It is found that rack shortage rate is decreased up to 60% of decreased demands. However, no difference is found at above 70%. Waiting time tends to be longer with increased common zone while travel time and throughput are not affected at all with the common zone size.