한우의 혈액형 분류를 위한 표준혈청의 생산 및 혈액형 분류

권종국(J . K . Kwun),이근상(K . S . Lee),김희석(H . S . Kim),양보석(B . S . Yang),신형두(H . D . Shin),이완(W . Lee),이장헌(J . H . Lee),한호재(H . J . Han) 한국축산학회 1988 한국축산학회지 Vol.30 No.7

These experiments were carried out to establish the base of blood typing in Korean Native Cattle. The isoimmunization in cattle was performed to produce standard antisera for blood typing. Fifty-six kinds of standard reagent standardized by International Society for Animal Blood Group Research donated from Blood Typing Laboratory, Livestock Association of Japan, INC. were used as the reference reagents in these experiments. 1. Seven kinds of standard reagents, B and D of B system, R₂ and W of C system, U` and S of S system, Z of Z system, for blood typing were produced in this laboratory by isoimmunization and adsorption methods using ninety-five cattle. 2. The appearance frequencies of antigenic factors of RBC of two hundred and thirteen Korean Native Cattle were different from European cattles. Also, considerable frequency differences between Korean Native Cattle and the other kinds of East Asian tattles were recognized. 3. The gene frequencies of serum biological polymorphism in two hundred and thirteen Korean Native Cattle were as follows: A1bA: 0.974, A1bB: 0.026 in albumin locus, AmB: 0.306, AmC: 0.694 in amylase locus, CaF: 0.067, CaS: 0.933 in carbonic anhydrase locus and CpF: 0.340, CpS: 0.645, CpI: 0.015 in ceruloplasmin locus.

재래산양(在來山羊)에 있어서 PBI¹³¹ Conversion Ratio 와 I¹³¹의 대사(代謝)

권종국,성재기,이영소,이용빈,Kwun, J.K.,Sung, J.K.,Rhee, Y.S.,Lee, Y.B. 대한수의학회 1966 大韓獸醫學會誌 Vol.6 No.1

The protein-bound iodine-131, the concentration of iodine-131 in blood, and the excretion rate of I-131 through urine and feces were observed in nine Korean native goats, 3 months age, following administration of $3{\mu}C$ of I-131 per kg of body weight. No signiant differences were found due to sex and castration. 1. The average protein-bound iodine-131 conversion ratio of goats was 16.7% in 24 hours. In castrated group, the lowest proteinbound iodine-131 conversion ratio was observed. 2. The average concentration of iodine-131 in bleed, increased very rapidly by 2 hours(4.75%) and rapidly decreased within 6 hours(0.73%). 3. The average excretion rate of I-131 through urine was highest in 24 hours(19.00%) and decreased rapidly within 48 hours(5.32%). 4. The average excretion ration rate of I-131 though feces was highest in 24 hours(2.55%), and decreased slowly.

흰쥐 자궁 및 착상에 있어서 Tamoxifen과 성스테로이드 호르몬의 상호관계

한호재,유규연,권종국,양일석,Han, H.J,Yoo, K.Y.,Kwun, J.K.,Yang, I.S. 대한생식의학회 1988 Clinical and Experimental Reproductive Medicine Vol.15 No.2

These studies were undertaken to examine the relationship between tamoxifen and sex steroid hormones in rat uterine morphology and the effect of tamoxifen on sex steroid hormone levels, implantation and myometrial contraction. The results obtained were as follows : 1) The increase in height of the luminal epithelium caused by tamoxifen treatment was blocked by progesterone. The increase in height of luminal epithelium caused by $estradiol-17{\beta}$ treatment was blocked by tamoxifen. 2) When a single dose of tamoxifen(10, 20, $40{\mu}g$) was given on Day 2 of pregnancy, implantation was prevented. Plasma $estradiol-17{\beta}$ level fell in a dose-dependent manner but plasma progesterone level was constant. 3) In vitro, tamoxifen decreased rat uterine contractility in a dose-dependent manner.

표준항혈청 생산 및 한우 ( Bos taurus coreanae ) 의 혈액형에 관한 연구

신형두,윤충근,신언익,양일석,권종국 ( H . D . Shin,C . K . Yoon,U . I . Shin,I . S . Yang,J . K . Kwun ) 한국축산학회 1993 한국축산학회지 Vol.35 No.4

These studies were carried out to produce standard antisera for the serological blood typing of cattle and to clarify the genetic characteristics of Korean Native Cattle. For the serological blood typing, thirty-three standard antisera(reagents) were produced by iso-immunizations and absorptions with two hundreds animals. Seven hundreds and forty-two Korean Native Cattle were bloodtyped by those antisera. Also the phenogroups (alleles) in each blood group system were investigated. Two hundreds animals were bloodtyped with standard antisera which introduced from bovine bloodtyping laboratory, Saskatoon, Canada and used far isoimmuniz anon to produce immunesera. Thirty-two reagents(A1, A2 and Z` of A system; B, C, I1, Y2, A`1, A`2, B`, D`, E`2, E`3, I` and J` of B system; C1, C2, X1, X2, R1, R2, W and L` of C system; F and V of I` system; J of J system; S, H`, U1, U`1, U` and U$quot; of S system: Z of Z system) were produced by iso-immnunizations and absorptions. And J reagent was seperated from natural serum. In ISAG international comparison test in 1991, the specificities of these thirty-three antisera were compared with those of other laboratory. In A blood system, four kinds of phenogroups were appeared. The gene frequency of A1Z` phenogroup, which has been characteristically reported in Indian cattle breed, was 0.206. In B blood system, thirty-five kinds of phenogroups were appeared and showed similar gene frequencies in each other. In C blood system, sixteen kinds of pheuogroups were appeared. The gene frequency of X2 phenogroup was the highest(0.300). In F blood system, three kinds of alleles were appeared. The gene frequency of $quot;-$quot; allele which has been characteristically reported in Indian cattle breed, was 0.213. In S blood system, ten kinds of phenogroups were appeared. The gene frequency of $quot;-$quot; and G$quot; phenogroups were relatively high. In J and Z blood system, the gene frequency of J and Z alleles were 0.432 and 0.600.

한우 ( Bos taurus coreanae ) 의 혈액단백다형에 관한 연구

신형두(H . D . Shin),신언익(U . I . Shin),양일석(I . S . Yang),권종국(J . K . Kwun) 한국축산학회 1993 한국축산학회지 Vol.35 No.3

These studies were carried out to clarify the genetic characteristics of Korean Native Cattle. To examine the biochemical polymorphisms of eight protein loci, polyacrylamide and starch gel electrophoresis methods were used. Gene frequencies of alleles in hemoglobin locus(Hb) were 0.870(Hb A) and O.130(Hb B) which was rarely reported in European cattle breed. Gene frequencies of alleles in albumin locus(Alb) were 0.997(AIb A) and 0.003(Alb B) which was rarely reported in European cattle breed. Gene frequencies of alleles in amylase t locus (Am I) were 0.307(Am I B) and 0.693(Am I C). Gene frequencies of alleles in carbonic anhydrase locus(CA) were 0.065(CA F) and 0935(CA S). Gene Irequencics of alleles in ceruloplasmin locus(Cp) were O.s38(Cp A) and 0.662(Cp C). Gene frequencies of alleles in post transferrin-2 locus(pTf-2) were 0.692(pTf-2 F) and 0.30 8(pTf-2 S). Gene frequencies of alleles in post-albumin locus(pAlb) were 0.580(pAlb F) and 0.420(pAlb S). Gene frequencies of alleles in transferrin locus(Tf were 0.268(Tf A), 0.234(Tf D1), 0.247(Tf D2), 0.235(Tf E), 0.017(Tf K) and 0.001(Tf Y), respectively. The two transferrin variants have been found in this studies and nomenclatured as Tf K and Tf Y. The heredity of Tf K allele were reported at first.

한우혈액형의 phenogroup 과 아형관계에 관한 연구

신형두(H . D . Shin),신언익(U . I . Shin),양일석(I . S . Yang),권종국(J . K . Kwun) 한국축산학회 1990 한국축산학회지 Vol.32 No.9

This experiment was carried out to clarify the phenogroups and subtype relationships of bloodtype antigens appeared in Korean Native Cattle. Fifty-two standard antisera(reagents) which were standardized by International Society for Animal Blood Group Research were introduced for bloodtyping of 213 Korean Native Cattle. And computer program II-2 and I-2 were used to find phenogroups. 1. In A system, 4 kinds of phenogroups; A₁, A₂ A₁Z` and $quot;-$quot; were appeared in Korean Native Cattle and A₁ Phenogroup showed the highest gene frequency(0.5065). Z`-A₁-A₂ linear subtype relationship was proved in Korean Native Cattle. 2. In B system, 49 kinds of phenogroups were appeared in Korean Native Cattle. And B-K, linear subtype relationships and A`₁-A`₂ A`_x, non-linear subtype relationship were approved. In G₁-G₂-G_x non-linear subtype relationship, G₂ antigens without G₁ and G_x antigens were appeared. 3. In C system, C₁-C₂. R₁-R₂ linear subtype relationship and X₁-X₂-X` non-linear subtype relationship were appeared in Korean Native Cattle. 4. In F system, 7 kinds of phenogroups : F, V₁, V₂, FN`, V₁N`, FV₂N` and $quot;-$quot; were appeared in Korean Native Cattle and FV₂N` phenogroup which had not emerged in other species of East Asian cattle showed gene frequency of 0.0013. In V₁-V₂ linear subtype relationship, V₁ antigens without V₂ antigens were appeared in Korean Native Cattle. 5. In S system, 5 kinds of phenogroups : H`U₁, SH`, H`, U` and $quot;-$quot; were appeared in Korean Native Cattle and H` and U` phenogroups showed relatively high gene frequencies, 0.2523 and 0.2614, respectively. U₁-U₂-U` non-linear subtype relationship was appeared in Korean Native Cattle. 6. In J, L, Z and R`-S` system, two kinds of alleles were appeared in Korean Native Cattle, respectively. The gene frequencies of J. L, Z and R`-S` alleles were 0.1279, 0.0383, 0.4827 and 0.0024, respectively.

혈액단백다형에 의한 지역별 한우의 유전거리에 관한 연구

신형두(H . D . Shin),이득환(D . H . Lee),신억익(U . I . Shin),양일석(I . S . Yang),권종국(J . K . Kwun) 한국축산학회 1993 한국축산학회지 Vol.35 No.5

These studies was carried out to clarify the genetic relationships of Korean Native Cattle by blood protein polymorphisms. Heterozygosity, genetic identity and genetic distance among five regional groups of Korean Native Cattle were estimated and dendrograms were constructed by principal component analysis and cluster analysis. The genetic differences among regional groups of Korean Native Cattle were not significant but Korean Native Cattle could be classificated in two groups of Seosan-Jeonla-Chungchoung and Kangwon-Kyoungsang by dendrogram. It was found, by dendrogram using data of other reports, that the genetic influence of Indian breed on Korean Native Cattle was slight.