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추출방법에 따른 돼지감자 잎의 항산화 및 생리활성 비교
김재원(Jae-Won Kim),김종규(Jong-Kyoun Kim),송인성(In-Seong Song),권은성(Eun-Sung Kwon),윤광섭(Kwang-Sup Youn) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.1
돼지감자 잎의 활용 및 생리활성을 증가시킬 수 있는 적정추출방법을 알아보고자 환류냉각, 상온교반, 가압가열 및 저온고압 추출법을 이용하여 추출한 돼지감자 잎 추출물의 생리활성을 비교하였다. 추출수율은 가압가열추출, 환류냉각추출, 상온교반추출, 저온고압추출 순으로 높은 수율을 나타내었다. 폴리페놀 함량은 상온교반추출 및 환류냉각추출의 경우 대등한 함량을 나타낸 반면 가압가열추출 및 저온고압 추출에서는 유의적으로 높은 함량을 나타내었으며, 플라보노이드 함량은 가압가열추출 및 저온고압추출에서 높은 함량을 나타내었다. Proanthocyanidin 함량에서는 저온고압추출에서 가장 높은 함량이 검출된 반면 가압가열추출에서는 상온교반 및 환류냉각추출에 비해 낮은 함량을 나타내었다. 항산화 활성에서는 모든 추출물이 농도가 증가함에 따라 활성은 비례적으로 증가하였다. 가압가열추출 및 저온고압추출에서 유의적으로 높은 활성을 나타내었으며, α-glucosidase,xanthine oxidase 및 angiotensin I-converting enzyme 저해활성에서는 저온고압추출물에서 우수한 활성을 나타내었다. 이러한 결과를 종합해 볼 때 가압가열 및 저온고압추출물이 소재 활용가치가 높을 것으로 사료되며 천연 항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다. The physiological properties of water extracts from Jerusalem artichoke (Helianthus tuberosus L.) leaves (JAL) with different extraction processes (stirrer extraction, SE; reflux extraction, RE; autoclave extraction, AE; low temperature high pressure extraction, LTPE) were investigated. The freeze-dried powder yields of SE, RE, AE, and LTPE were 22.33%, 29.88%, 31.65, and 15.74%, respectively. AE showed the highest value of extract yield. The a* and b* values were higher in AE compared to other extracts. Total polyphenolics and flavonoids contents in AE was significantly higher than in other extracts. The amount of proanthocyanidin related substances were highest in LTPE (29.36 mg/g), followed by RE (21.57 mg/g), SE (20.35 mg/g), and AE (13.02 mg/g). The electron donating abilities of SE, RE, AE, and LTPE at a concentration of 500 μg/mL (w/v) were 76.16%, 39.55%, 25.50%, and 12.59%, respectively. Reducing power for the four different processes was 1.79, 1.60, 1.51, and 1.17, respectively. Additionally the same tendency was observed with electron donating ability and reducing power for ABTS radical and nitrite scavenging abilities. AE and LTPE showed relatively high antioxidant activities. Alpha-glucosidase, xanthine oxidase, and angiotensin I-converting enzyme inhibitory ctivities of LTPE at a concentration of 500 μg/mL (w/v) were somewhat higher than other extracts. Additionally, there was significantly higher or little lower inhibitory activity compared to the control group. In conclusion, we provided experimental evidence that extracts of JAL have potential as functional materials, and component analysis of JAL could be used as new cosmeceuticals. Also, LTPE is the superior method for the enhancement of biological activity.
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식도에서 발생한 Angiocentric T-Cell Lymphoma 1예
김영수,신용운,이돈행,김범수,황태숙,배성권,주명선,조현근,권은성,최원 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.4
In this study we present a case of an angiocentric T-cell lymphoma (ACTL) which involve the esophagus. A 37-year-old male was admitted with dysphagia, odynophagia, and weight loss for 3 months. Two months before his admission, he had undergone on endoscopy with a biopsy. The first endoscopic findings revealed a large ulcerative lesion on the esophagus, although there had been no evidence of lymphoma upon microscopic examination. On the follow up endoscopic examination, this ulcerative lesion revealed progressive and destructive change. Histopathology of the third biopsied specimens showed large atypical lymphocyte infiltrates with angiocentric and angioinvasive features. Immunohistochemical studies revealed that the atypical (large) lymphocytes had T-cell phenotypes (CD3+, CD5+, and CD43+) and contained few reactive B-cells (CD20+), We assume that this is the first case of ACTL involving the esophagus and larynx.
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