효모 미토콘드리아의 K + 활성화 알데히드탈수소효소의 정제와 반응메카니즘에 관한 연구

구본훈,주충노 ( Bon Hoon Koo,Chung No Joo ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

Yeast (Saccharomyces cerevisiae) mitochondrial K^+ activated aldehyde dehydrogenase (ALDH) was purified and the effect of Li^+ and Me^(2+) was investigated. It was found that both 50 mM Li^+ and Mg^(2+) inhibited the dehydrogenase reaction of ALDH as much as 50% and 76% respectively while the esterase reaction of this enzyme was stimulated 13% and 8%. This suggested that Li^+ and Me^(2+) stimulated deacylation of the intermediate thioester but inhibited the dissociation of Enzyme-NADH complex and the K^+ activation might be involved in the dissociation of Enzyme-NADH complex which seemed to be the rate limiting step in the ALDH catalyzed reaction.

효모 미토콘드리아의 $K^+$-활성화 알데히드탈수소효소의 정제와 반응메카니즘에 관한 연구

구본훈,주충노,Koo, Bon-Hoon,Joo, Chung-No 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

효모 미토콘드리아의 $K^+$-활성화 ALDH을 정제하고 $Li^+$와 $Mg^{2+}$의 영향을 조사한 결과 금속이온의 농도가 50mM일 때 ALDH의 dehydrogenase 활성은 각각 50%, 76% 감소하였으나, esterase 활성은 각각 13%,8%씩 오히려 증가하였다. 이와 같은 실험결과는 $Li^+$와 $Mg^{2+}$가 deacylation을 촉진하는 반면 E-ALDH 복합체로부터의 NADH 분리를 억제하는 것으로 해석되고, $K^+$이 주로 E-NADH 복합체로부터의 NADH 해리반응에 관여함을 뜻하며 E-NADH 복합체로 부터의 NADH의 해리 단계가 효모 미토콘드리아 ALDH 반응의 속도제한 단계일 가능성이 큰 것으로 생각된다. Yeast (Saccharomyces cerevisiae) mitochondrial $K^+$ activated aldehyde dehydrogenase (ALDH) was purified and the effect of $Li^+$ and $Mg^{2+}$ was investigated. It was found that both 50mM $Li^+$ and $Mg^{2+}$ inhibited the dehydrogenase reaction of ALDH as much as 50% and 76% respectively while the esterase reaction of this enzyme was stimulated 13% and 8%. This suggested that $Li^+$ and $Mg^{2+}$ stimulated deacylation of the intermediate thioester but inhibited the dissociation of Enzyme-NADH complex and the $K^+$ activation might be involved in the dissociation of Enzyme-NADH complex which seemed to be the rate limiting step in the ALDH catalyzed reaction.

간 질환을 가진 환자의 정신약물요법

구본훈(Bon-Hoon Koo) 대한생물치료정신의학회 2008 생물치료정신의학 Vol.14 No.2

간은 인체 내에서 약물을 대사시키는 역할을 하기 때문에 임상적 관점에서 가장 중요한 기관 중의 하나이다. 간 기능장애 시 대사과정의 장애로 인한 독성물질 때문에 신경학적 증상과 정신과적 증상이 혼합되어 나타나기도 한다. 또한, 간 질환을 가진 환자에게서 우울, 불안, 섬망, 알코을 및 물질관련장애 등의 여러 가지 정신과질환이 같이 동반되기도 한다. 본 논문은 간 질환을 가진 정신과환자의 약물치료에 있어서 임상의사가 고려해야할 주제들을 정리하여 임상에서 도움을 얻고자 하였다. 간에서의 약물 대사는 phase Ⅰ과 phase Ⅱ의 두 가지 대사 과정으로 나눌 수 있다. 간에서의 약물의 청소(clearance)는 간 효소(hepatic enzyme)에 대한 약물의 전달 정도(rate of delivery)나 또는 효소의 내적인 역량(intrinsic capacity)에 의해 영향을 받을 수 있다. 간 질환을 가진 환자에서의 약물 사용 시 약물의 흡수, 분포, 대사 및 배설에서의 변화가 나타날 수 있다. 대부분의 정신과약물은 일차적으로 간에서 대사되기 때문에 간 기능장애는 정신과약물의 청소(clearance)를 감소시키고 약물의 혈중 농도를 변화시킨다. 따라서, 정신과의사는 간 질환을 가진 환자에게서 정신과 약물의 부작용이 더 흔히 나타날 수 있다는 점을 명심하고 이에 대해 주의 깊게 관찰을 해야 한다. 간 질환을 가진 환자에게 정신과약물을 사용할 때는 초기에 낮은 용량으로 시작하고 좀더 천천히 약물의 용량을 증량해야 한다. 그러나 대부분의 정신과약물의 상호작용은 임상적으로는 크게 문제가 되는 않는 것으로 알려져 있다. Liver is the key organ to metabolize various drugs and nutrients. It is very important to know in clinical perspective that liver dysfunction leads to change the level of the drugs. Toxic materials due to liver dysfunction cause several neurological and psychiatric symptoms such as fatigue, change of mood & personality, and memory disturbances. Patients with liver diseases also have comorbid psychiatric disorders such as depressive disorders, anxiety disorders, alcohol and other substance use disorders. The purpose of this article is to review about the issues of psychopharmacotherapy of patients with liver diseases. The metabolic process of the drugs in liver are divided into phase I and phase Ⅱ metabolism. The clearance of the drugs depend on the rate of delivery of the drugs to hepatic enzymes, and the intrinsic capacity of hepatic enzymes. There can be changed of absorption, distribution, metabolism, and excretion of the drugs when we use psychiatric drugs in patients with liver diseases. Because the most psychiatric drugs are primarily metabolized in liver, liver dysfunction can reduce the clearance of the drugs, and change the blood level of the drugs. The issues of each psychiatric drugs are discussed in this article. Psychiatrists should be cautious to develop adverse effects of the psychiatric drugs, and need to monitor carefully. The initial doses of psychiatric drugs in patients with liver diseases should be reduced and titrated more slowly than medically healthy patients. But the interactions of most psychiatric drugs in patients with liver diseases are not clinically significant.

ADHD 아동의 한국판 K-ABC 수행특성: 2. 정보처리 유형에 따른 분석

구본훈 ( Bon Hoon Koo ),이혜린 ( Hye Lin Lee ),배대석 ( Dai Seg Bai ) 한국정서.행동장애아교육학회(구.한국정서학습장애아교육학회) 2006 정서ㆍ행동장애연구 Vol.22 No.4

외상성 두부손상 환자에서 지능장애 및 기억장애 유무에 따른 정신과적 증상의 비교

구본훈(Bon Hoon Koo),배대석(Dai Seg Bai) 대한생물치료정신의학회 2006 생물치료정신의학 Vol.12 No.2

외상성 두부손상 환자들의 지능장애와 기억장애의 유무에 따라 인지기능장애의 양상과 정신과적 증상의 양상을 알아보기 위하여, 1999년 3월 1일부터 2004년 12월 31까지 두부외상 후 정신과적인 증상을 나타내어 진료를 받았거나 자문이 의뢰된 경우 및 후유장애 평가를 위해 내원한 환자 중 배제기준을 제외한 161명을 대상으로 K-WAIS, K-MAS, K-BNT, SCL-90-R, MMPI를 실시하였다. K-WAIS와 K-MAS, 그리고 K-BNT의 경우, 지능장애와 기억장애를 모두 동반한 군에서 나머지 두군보다 전반적으로 더 많은 인지장애를 보였고 특히, 주의력을 포함한 군집화와 침입화 등의 기억전략의 문제를 보였다. SCL-90-R의 경우, 지능장애와 기억장애를 모두 동반한 군에서 정신병적 경향을 포함하여 정신과적 증상의 종류와 정도를 가장 많이 보이고 있었으며, 기억장애만을 동반한 군에서는 지능장애나 기억장애를 동반 하지 않은 군과 별다른 차이를 보이지 않았다. MMPI의 경우, 지능장애와 기억장애를 모두 동반한군에서 정신과적 문제의 정도나 정신병리가 더 심한 것으로 나타났으며, 기억장애만을 동반한 군에서는 정신과적 증상에 대해 다소 방어적인 태도를 나타내었다. 또한, 하위척도 중 우울증척도에서는 인지장애의 동반 유무와 별다른 차이를 보이지 않았다. 따라서 기억장애만을 보이는 군의 경우, 정신과적 증상을 직접적으로 드러내지 않을 가능성이 있으며 임상현장에서 이러한 점에 대한 고려가 필요할 것으로 생각된다. Objectives:The purpose of this study was to investigate the clinical characteristics of psychiatric symptom in traumatic head injury with and without intelligence and memory impairments. Methods:The patients was divided into three groups according to differences between intelligence and memory impairments, such as, the group who has both intelligence and memory impairments(AD), the group who has only memory impairments(MD), and the group who has no or mild intelligence and memory impairments(ND). We compared with their clinical characteristics of psychiatric symptom by each groups using K-WAIS, K-MAS, K-BNT, SCL-90R, MMPI. Results:In the results of K-WAIS, K-MAS, and K-BNT, AD was shown more severe cognitive impairments than other groups, especially, certain kind of memory strategies including attention, clustering, and intrusions. In the results of SCL-90R, AD showed significant differences in subscale and global index than other groups, but there was any differences between ND and MD. In the K scale score in MMPI, MD has higher scores than other groups. Conclusion:In this research, both intelligence and memory function damaged group has experienced more severe cognitive and emotional dysfunction. The group with only impaired memory function has also psychiatric symptoms but they tend to more defensive attitude to the psychiatric symptoms.

ADHD 진단을 위한 전산화 신경인지 기능검사의 유용성

구본훈(Bon Hoon Koo),배대석(Dai Seg Bai) 대한생물치료정신의학회 2006 생물치료정신의학 Vol.12 No.1

ADHD의 진단 및 치료효과 평가를 위한 검사도구로서의 가치를 알아보기 위해 120명의 정상아동 집단, 31명의 ADHD 집단 및 16명의 ADHD가 소아정신장애 집단을 대상으로 하여 세 집단 간의 전산화 신경인지기능검사 결과를 비교하였고, 그 변별력을 알아보았다. CNT의 주의력검사와 기억력검사 그리고 고위인지기능검사에서 ADHD 집단의 경우 대체로 정상집단과는 유의하게 차이가 있었으나 비 ADHD 집단과는 유의한 차이가 없었다. CNT의 정상아동 집단과 ADHD 집단 및 비 ADHD 집단에 대한 판별분석에서 정상아동에 대한 특이도가 99.0%, ADHD에 대한 민감도가 55.6%, 비ADHD에 대한 민감도가 66.7% 그리고 전체 판별 정확도가 89.5%임을 확인하였다. 따라서 CNT는 ADHD의 진단적인 보조도구로서 유용하며, 경과관찰 및 치료효과를 정량적으로 볼 수 있는 유용한 도구로 생각한다. Objective : The focus of this paper is to examine the utility of the Computerized Neurocognitive Function Test(CNT) system for the diagnosis and assessment of therapeutic effect in children with Attention Deficit Hyperactivity Disorder(ADHD). Method : The Computerized Neurocognitive Function Test and Korean Kaufman Assessment Battery for Children were performed to 120 normal children, 31 children with ADHD, and 16 children with other neuropsychiatric disorders. And then compared with attention, memory and higher cognitive function among three groups. Result : Children with ADHD had significant differences compared to normal children in attention, memory and higher cognitive function, but there were no differences between children with ADHD and children with other neuropsychiatric disorders. In discriminant analysis for CNT among three groups, the specificity to normal children was 99.0%, the sensitivity to children with ADHD was 55.6%, the sensitivity to children with other neuropsychiatric disorders was 66.7%, and the accuracy of all groups was 89.5%. Conclusion : This study suggested that CNT is relatively valuable method and in the evaluation of children with ADHD. But further verification studies will be necessary.

열응력해석을 통한 재하시 H형강 휨재의 하중비에 따른 한계온도 산정

구본훈(Koo, Bon-Hoon),윤성기(Yoon, Sung-Kee) 대한건축학회 2014 대한건축학회 학술발표대회 논문집 Vol.34 No.2

재하된 H형강 휨재의 열응력해석을 이용한 한계온도 산정

윤성기,이치형,구본훈,Yoon, Sung Kee,Lee, Chy Hyoung,Koo, Bon Hoon 한국강구조학회 2015 韓國鋼構造學會 論文集 Vol.27 No.4

국내의 내화성능평가는 대부분 사양적설계 방법인 품질시험을 통해 이뤄지고 있다. 하지만 시험 특성상 많은 비용과 시간을 요구하며 다양한 건축구조에 대한 내화성능평가에는 어려움이 많다. 따라서 본연구에서는 성능적 설계를 위한 연구로 유한요소 구조해석 프로그램인 ABAQUS를 사용해 H형강 휨재를 대상으로 변수에 따른 열응력 해석을 통해 내화성능을 검토하며, 하중비별 한계온도를 제안한다. The domestic fire resistance performance test is conducted as a prescriptive design method such as quality test. In quality test there are 2 methods, unloaded fire resistance test and fire resistance test under load. In realistic, these tests, however, have problems with expense, time and diversity of structure. This study reviewed fire resistance performance of H-beam flexural member by thermal stress analysis using finite element ABAQUS program. This research is for the performance-based design reviewing applicability of domestic standard. As a result of this study, limit temperatures per each load ratio provied for proper performance of fire resistancy.

대구지역 일개 초등학교를 대상으로 한 소아 수면문제에 대한 예비연구

서완석(Wan Seok Seo),구본훈(Bon Hoon Koo),김민지(Min Ji Kim),노영환(Yeung Hwan Rho),성형모(Hyung-Mo Sung),신지현(Ji-Hyun Shin) 대한소아청소년정신의학회 2008 소아청소년정신의학 Vol.19 No.3

Objectives: The purpose of this study was to investigate the prevalence of sleep problems in Korean elementary school children and the differences in sleep problems elated to their school grade and gender. Methods : One public elementary school in Dae-gu, Korea as randomly selected. And, parents who agreed to participate this study were asked to complete a modified version of Tucson Childrens Assessment of Sleep Apnea screening questionnaire (TuCASA). Results: The most common sleep problem was falling asleep in vehicle (44.2%) and the prevalence rate of this problem differed by school grade. The prevalence rate of falling asleep while watching TV before 8 P.M. (8.0%), daytime fatigue (21.9%) and nocturnal enuresis during the past 6 months (5.3%) were significantly differed by school grade. The prevalence rate of falling asleep while doing home work, falling sleep during a lesson, snoring and bruxism were 12.6%, 1.0%, 26.7% and 13.2% respectively. Although these rates did not show any differences between grades, snoring as more in boys and daytime fatigue was more in girls. Conclusion: This study demonstrates that many elementary school children may have various sleep problems. Lower grade elementary schoolers had sleep problems which related to euro-developmental factors, whereas higher grade elementary schoolers had sleep problems related to sleep deprivation and stress. Finally, more female schooler showed signs of fatigue or sleepiness than male schoolers.

국내 대학병원 공황장애 환자들의 임상적 특징에 대한 연구 : 다기관, 후향적 연구

오재영,이재헌,한상우,지익성,구본훈,우종민,양종철,김민숙,이상혁,허정윤,유범희,Oh, Jae-Young,Lee, Jae-Hon,Han, Sang-Woo,Chee, Ik-Seung,Koo, Bon Hoon,Woo, Jong Min,Yang, Jong-Chul,Gim, Min-Sook,Lee, Sang Hyuk,Heo, Jung-Yoon,Yu, Bum-Hee 대한불안의학회 2014 대한불안의학회지 Vol.10 No.1

Objective : Despite the high prevalence and clinical importance of panic disorder, studies on the clinical characteristics and course of panic disorder are relatively rare. This study is a multi-center, and retrospective study to examine the clinical characteristics and course of Korean panic disorder patients who visit university hospital. Methods : The study subjects were panic disorder patients who had visited the psychiatric outpatient clinics of 8 university hospitals in South Korea from January to December in 2008. Finally, 238 panic patients were included in this study. Their medical charts were retrospectively reviewed and reassessed by experienced psychiatrists to examine their clinical characteristics, demographic data and clinical course in repose to pharmacotherapy. Results : Among the 238 patients (121 males vs. 117 females), the mean age of disease onset was $41.3{\pm}12.7$ years and female patients showed 5 years older age of disease onset, compared with male patients. The mean score of PSR scale was $4.5{\pm}1.0$ at the first visit, reflecting a 'marked' level of severity of illness and impairment in functioning. Only 110 patients (46.4%) completed the whole follow up visits, whereas 128 patients (53.6%) dropped out during the treatment. After $17.7{\pm}0.5$ months of mean follow up period, the mean score of PSR scale at the last visit was reduced into $2.1{\pm}0.9$, reflecting a 'residual' severity of illness and impairment in functioning. The cumulative recovery rate was 62.1% in the completer group, whereas that of the drop-out group was 47.7%. Conclusions : The mean age of disease onset in Korean panic disorder patients who had visited university hospital was about 10 years older than that of Western panic disorder patients in previous studies, and the Korean panic disorder patients who had visited university hospital showed a relatively higher cumulative recovery rate. These differences might result from an ethnic difference in clinical characteristics and course in response to pharmacotherapy of panic disorder.