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이태식(lee Tai-Sik),강지훈(Kang Ji Hoon),곽병문(Kwak Byung-Moon),김근수(Kim Kuen-Soo),이석용(Lee suck-Yong) 한국철도학회 2007 한국철도학회 학술발표대회논문집 Vol.- No.-
In recently, our social is entering to service infinite competitive time., So If companys don"t give satifactory and an impression customers, they don"t exist. The Companies that operate railway are promoted(Seoul metro, , The Korail, Seol metropolitan Rapid Transit Corperation, Incheon Rapid Transit Corporation), they are into a stronger competitor. So the service for customer is more inportant to improve the image and administration than before. Especially, station workers who work near the customer are important for cooperation image. But, there are only a few employees to operate the station like ticketing in railway station, There aren"t reinforcement the service employees and expanse the utility system. especially, since the transportation card is introduced in 1996, the customers who use the T-Money card are increased. So the change of service system is necessary to provide a substantial service to customer. This paper derives the problems through analysising the operating system of station, and presents the improvement to provide substantial services to customer.
김형묵,김태준,임동빈,하순봉,김이화,차병선,허효진,소피아브리토,이용문,빈범호,곽병문,Kim, Hyung-Mook,Kim, Tae-Jun,Im, Dong-bin,Ha, Sun-Bong,Kim, Ee-Hwa,Cha, Byung-Sun,Heo, Hyo-Jin,Brito, Sofia,Lee, Yong-Moon,Bin, Bum-Ho,Kwak, Byeong-Mun 대한한방안이비인후피부과학회 2021 한방안이비인후피부과학회지 Vol.34 No.4
Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.