Taurine 및 ${\beta}-alanine$이 고양이 척수후각세포의 Activity에 미치는 효과

고영익,강석한,김진혁,신홍기,김기순,Koh, Young-Ik,Kang, Sok-Han,Kim, Jin-Hyuk,Shin, Hong-Kee,Kim, Kee-Soon 대한생리학회 1990 대한생리학회지 Vol.24 No.1

In 19 cats anesthetized with ${\alpha}-chloralose$ effects of taurine and ${\beta}-alanine$ on the responses of wide dynamic range (WDR) cells to mechanical, chemical and thermal stimuli were investigated in the lumbar spinal cord of the cat. Also studied was an interaction of strychnine with taurine in affecting the activities of WDR cells. Following intravenous administration of taurine, the responses of WDR cells to all types of mechanical stimuli were markedly enhanced, demonstrating that the response to pressure was most sensitive to taurine action. When the receptive field was exposed to thermal stimuli ($50^{\circ}C$) for 20 sec. taurine increased activity of WDR cell to 169.5% of the control value. The $K^{+}$-induced activation of WDR cells was invariably suppressed after taurine administration. Intravenously administered strychnine remarkably reduced the enhanced response of WDR cell to natural stimuli resulting from intravenous administration of taurine. Also ${\beta}-alanine$ markedly activated the response of spinal dorsal horn cell to natural mechanical stimuli. These findings suggest that neutral amino acid and its derivative such as ${\beta}-alanine$ and taurine can enhance the response of WDR cells to different stimuli in cats.

임신 첫 3개월간 산전진단방법으로서의 융 모막 융모 샘플링

고영익,차상헌,이권해,조태호 대한산부인과학회 1987 대한산부인과학회 학술대회 Vol.60 No.-

자궁외 임신의 임상적 고찰

이민철,이임순,김영채,조태호,고영익 순천향대학교 1989 논문집 Vol.12 No.1

This study is clinico-statistical analysis and survey of 219 cases who were admitted and treated at Soonchunhyang university hospital from May 1, 1974 to Feb, 28, 1988. The result were as follows: 1. An incidence of ectopic pregnancy was 1 in 90 deliveries. 2. The most frequent age group was in 25 to 29 years of age, comprising of 34.7%. 3. Nulligravida was 11.9%, nullipara 37.4% and multipara 62.6% and the case who had a previous history of curettage for abortion was 62.6%. 4. In past history, comprising of 20.5% previous laparotomy, 5.9% P,I.D., 5.5% laparoscopic tubal ligation, 1.4% I.U.D.… 5. In sign and symptom, lower abdominal pain was in 95.4%, vaginal spotting in 68.9%, nausea and vomiting in 14.2%. The most common interval between L.M.P. and onset of symptoms was 5-6 weeks in 40.2%. The mean initial Hb level was 10.8 mg %. 6. Culdocentesis was positive in 80.1% and gravindex test was positive in 62.3%. 7. Site of implantation were in the tubes 95.9%, in the ovary 1.8%, in the cervix 1.8%, in the abdomen 0.5%. In termination of tubal pregnancy, tubal rupture was noted in 70.3%, tubal abortion in 23.7%, and intact cases in 6.0%. 8. The total amount of intraperitoneal hemorrhage between 1,000 to 2,000 cc was in 62.5%, above the 2,000 cc was in 2.7%, and less the 1,000 cc was in 34.8%. 9. 58.9% of total cases were treated by salpingectomy, 30,1% by salpingo-oophorectomy and 2.3% by cornual resection. 10. There was no deaths in 239 total ectopic pregnancies.

Taurine 및 ß-alanine이 고양이 척수후각세포의 Activity에 미치는 효과

고영익(Koh, Young-Ik),강석한(Kang, Sok-Han),김진혁(Kim, Jin-Hyuk),신홍기(Shin, Hong-Kee),김기순(Kim, Kee-Soon) 대한생리학회 1990 대한생리학회지 Vol.24 No.1

In 19 cats anesthetized with α-chloralose effects of taurine and β-alanine on the responses of wide dynamic range (WDR) cells to mechanical, chemical and thermal stimuli were investigated in the lumbar spinal cord of the cat. Also studied was an interaction of strychnine with taurine in affecting the activities of WDR cells. Following intravenous administration of taurine, the responses of WDR cells to all types of mechanical stimuli were markedly enhanced, demonstrating that the response to pressure was most sensitive to taurine action. When the receptive field was exposed to thermal stimuli (50℃) for 20 sec. taurine increased activity of WDR cell to 169.5% of the control value. The K⁺-induced activation of WDR cells was invariably suppressed after taurine administration. Intravenously administered strychnine remarkably reduced the enhanced response of WDR cell to natural stimuli resulting from intravenous administration of taurine. Also β-alanine markedly activated the response of spinal dorsal horn cell to natural mechanical stimuli. These findings suggest that neutral amino acid and its derivative such as β-alanine and taurine can enhance the response of WDR cells to different stimuli in cats.

임신 초기 유산시 태아조직 진단법으로서의 Papanicolaou 염색검사

고영철,고영익,차상헌,조태호 순천향대학교 1988 논문집 Vol.11 No.3

For the identification of fetal tissue in products of first trimester abortion, three methods were used by gross test, inverted microscopic method and Pap smear. In materials from 100 consecutive first trimester abortion, pregnancy was identified grossly in 57 cases(60.0%), microscopically in 88 cases(92.6%) and cytologically in 93 cases(97.9%). In 5 tubal pregnancies, no fetal tissue was identified in uterine contents. The cytologic method is easily performed and is rapid, sensitive, certain inexpensive and permanent. The tests for the identification of fetal tissue were found to be almost reliable, and their use improves not only the practice of early abortion but also the diagnosis and management of suspected ectopic pregnancy and problematic situations in which tissue is passed vaginally.

자궁외임신시 황체기능

이권해,조태호,이민철,구자두,고영익,윤길중 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.2

1988년 4월 1일부터 1989년 5월 31일까지 순천향대학병원 산부인과에 내원한 자궁외임신환자 32명을 대상으로 혈청 β-hCG와 혈청 progesterone을 측정하였고, 그 중 22명의 환자에서 수술시 복강내 혈액에서 β-hCG를 측정하여 파열형과 비파열형으로 나누어, 혈청 progesterone의 측정이 자궁외임신의 진단적 가치와 혈청 progesterone과 혈청 β-hCG, 혈청 progesterone과 다글라스와 β-hCG를 파열형과 비파열형으로 나누어 비교분석하여 자궁외임신에서의 황체기능에 대한 연구결과 다음과 같은 결과를 얻었다. 1. 자궁외임신의 위치와 파열여부를 보면, 연구대상은 32예로 모두 난관임신이었으며, 부위별로 보면 난관 팽대부가 23예(71.8%), 체부가 5예(15.6%), 협부와 간질부는 각각 2예로 팽대부가 가장 많았으며, 파열여부로 보면 파열형이 19예(59.4%), 비파열형이 13예(40.6%)였다(Table 1). 2. 각 군별 평균치를 보면, Table 2에서 보는바와 같이, 파열형에서 혈청과 복강내 혈액의 β-hCG는 각각 2906.37 ± 720.17 mIU/ml, 8187.67 ± 1257.25mIU/ml이었고, 비파열형에서 β-hCG는 각각 1376.62 ± 480.67mIU/ml, 8239.20 ± 2210.46mIU/ml였다. 그리고 혈청 β-hCG 및 복강내 β-hCG를 파열형, 비파열형으로 나누어 보면 통계학적 차이는 없었다. 종합하여 보면 혈청 β-hCG는 2606.52 ± 578.38mIU/ml이고, 복강내 β-hCG는 8205.14 ± 1129.51 mIU/ml로 복강내 혈액에서의 β-hCG가 혈액에서보다 현저히 높았다(P$lt;0.0001). 그리고 자궁외임신에서 progesterone 평균치는 4.67로 정상임신시 보다 현저히 낮았으며, 파열형(5.18 ± 0.84ng/ml)이 비파열형(4.27 ± 0.94ng/ml)보다 조금 더 높았으나 통계학적 의의가 없었다(p=0.392). 3. 비파열형에서 혈청 progesterone치와 혈청 β-hCG와의 상관관계는 Y=0.001X + 2.94(r=0.49, P=0.09)였고, 혈청 progesterone치와 다글라스와 β-hCG와 상관관계는 Y=0.0001X + 2.71(r=0.352, P=0.318)로 다글라스와 β-hCG보다 혈청 β-hCG가 비교적 상관관계가 있으나 통계학적 의의는 없었다(Fig. 1). 4. 파열형에서 혈청 progesterone치와 혈청 β-hCG와의 상관관계는 Y=0.0005X + 3.85(r=0.39, P=0.10)이고, 혈청 progesterone치와 다글라스와 β-hCG와의 상관관계는 Y=0.0004X + 2.72(r=0.54, P=0.07)로써 다글라스와 β-hCG와 비교적 상관관계가 있으나 통계학적 의의는 없었다. 5. 전체 자궁외임신에서 혈청 progesterone치와 혈청 β-hCG와의 상관관계는 Y=0.0002X + 4.08(r=0.21, P=0.24)로써 혈청 progesterone치와 다글라스와 β-hCG와 상관관계는 Y=0.0002X + 3.10(r=0.41, P=0.06)로써 다글라스와 β-hCG와 상관관계가 있으나 통계학적 의의는 없었다. The purpose of this study was to know whether the serum progesterone level can serve as a diagnostic tool for ectopic pregnancy and to know luteal function for ectopic pregnancy. In attempt to evaluate luteal function in ectopic pregnancy, we measured serum β-hCG, cul-de-sac and serum progesterone level of 32 women with ectopic pregnancies who were admitted to Department of Obstetrics & Gynecology, Soonchunhyang University Hospital from April 1, 1988 to May 31, 1989. The results were obtained as follows: 1. The mean value of β-hCG level in ruptured ectopic pregnancy was 2906.37 ± 720.17 mIU/ml (Mean ± S.E.M) in serum and 8187.67 ± 1257.25 mIU/ml in cul-de-sac. The mean value of β-hCG level in unruptured group was 1376.62 ± 480.67 mIU/ml in serum and 8239.20 ± 2210.46 mIU/ml in cul-de- sac. When comparing the β-hCG level in serum of the ruptured group with that of the unruptured group, we had no significant value. Also when comparing the β-hCG level in cul-de-sac of the ruptured group with that of the unruptured group, we has no significant value. However, the β-hCG level in cul-de-sac (8205.14 ± 1129.51 mIU/ml) was significantly higher than serum β-hCG (2606.52 ± 578.38 mIU/ml)(p $lt; 0.001). The mean value of serum progesterone level in ectopic pregnancy was 4.67 ng/ml which was significantly lower than the value of serum progesterone in normal intrauterine pregnancy. The mean value of serum progesterone level in ruptured group (5.18 ± 0.84 ng/ml) was slightly higher than unruptured group (4.27 ± 0.94 ng/ml), but there was no significance. 2. In unruptured group, the serum β-hCG and serum progesterone levels were slightly correlated, but had no significance (r=0.49, p=0.09), and the cul-de-sac β-hCG and serum progesterone levels also had no significance. (r=0.35, p=0.32). 3. In ruptured group, the correlation between serum progesterone and serum β-hCG was no significance (r=0.39, p=0.1), and serum progesterone and cul-de-sac β-hCG was slightly correlated, but had no significance.(r=0.54,p=0.07) 4. In all ectopic pregnancies,the correlation between serum progesterone and serum β-hCG was no significance(r=0.03,p=0.24), and serum progesterone and cul-de-sac β-hCG was slightly correlated,but had no significance.(r=0.41,p=0.06).

자궁외임신 환자에 있어 다글라스와 혈액 β-hCG의 의의

조태호,이임순,이민철,고영익,최재호,양정분 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.6

1988년 4월 1일부터 1989년 5월 31일까지 순천향대학병원 산부인과에 내원한 자궁외임신 환자 29명을 대상으로 수술전 말초혈액과 수술시 복강내에 고여있던 혈액에서 β-hCG를 측정하여 파열형과 비파열형으로 나누어 비교분석하여 다음과 같은 결과를 얻었다. 1. 자궁외임신의 위치와 난관양상을 보면 연구대상 29예중 부위별로는 난관팽대부가 22예(76%), 체부가 5예(17.2%), 협부와 간질부는 각각 1예(3.4%)로 팽대부가 많았고, 난관양상은 파열형이 14예(48.2%), 비파열형 15예(51.8%)였다. 2. 혈청과 다글라스와 혈액에서의 β-hCG평균치를 비교시 혈청 2220.72 ± 661.888mIU/ml, 다글라스와 혈액 7338 ± 1331.83mIU/ml로 다글라스와 혈액에서 의미있게 높았고(P$lt;0.0001), 두 군에서 각각 파열형과 비파열형에 따른 β-hCG의 비교시 혈청에서는 파열형 3351.79 ± 1249.83mIU/ml, 비파열형 1165.73 ± 418.99mIU/ml, 다글라스와에서는 파열형 8499.29 ± 1694.30 mIU/ml, 비파열형 7155.73 ± 2067.11mIU/ml로 통계학적 의의는 없었다(P$gt;0.05). 3. 혈청 β-hCG치에 대한 다글라스와 혈액 β-hCG치의 비율의 범위는 최소 0.42에서 최고 85.93으로 평균은 13.35였고 혈청치가 낮을 수록 비율이 높아짐을 보이며, 난관 양상에 따라 비교하면 각각 9.78과 16.67로 비파열형에서 비율이 높았다. 4. 혈청과 다글라스와 혈액 β-hCG치의 상관관계는 파열형에서는 Y=1.05X + 4971.82(r=0.77, P$lt;0.05, N=14)로 상관관계가 높고 통계학적 의의도 있으나 비파열형에서는 Y=2.26X +4519.87(r=0.46, P=0.09, N=15)로 통계학적 의의는 없었다. 종합하면 Y=1.16X +5000.45(r=0.57, P$lt;0.05, N=29)로 통계학적 의의가 있었다. 이상에서 다글라스와 β-hCG를 측정하여 말초혈액의 β-hCG의 측정치와 비교함으로써 자궁외임신의 조기진단에 도움을 줄것으로 사료된다. The purpose of this study was to know whether the cul-de-sac blood β-hCG value serve as a tool for early diagnosis of ectopic pregnancy. Retrospective studies were made on serum β-hCG and cul-de-sac blood β-hCG values of 29 ectopic pregnancies, which were diagnosed by biopsy of surgical specimen, during 14 months from April 1, 1988 to May 31, 1989. The results were obtained as follows: 1. The mean value of β-hCG in ruptured ectopic pregnancy was 3351.79 ± 1249.81 mIU/ml (mean ± S.E.M.) in serum and 8499.29 ± 1694.30 mIU/ml in cul-de-sac blood. The mean value of β-hCG in unruptured group was 1165.73 ± 418.99 mIU/ml in serum and 7155.73 ± 2067.11 mIU/ml in cul-de-sac. When comparing the β-hCG level in serum of the ruptured group with that of the unruptured group, there was so significant value. Also when comparing the β-hCG level in cul-de-sac of the ruptured group with that of the unruptured group, there was no significant value. However, the β-hCG level in cul-de-sac (7338.83 ± 1331.83 mIU/ml) was significantly higher than serum β-hCG (2220.72 ± 661.88 mIU/ml). (P $lt; 0.0001). 2. The ratio of cul-de-sac to serum β-hCG had ranged from 0.42 to 85.93 and the mean ratio was 13.35. In according to decrease serum β-hCG level, the ratio was increased. The ratio was higher in unruptured group (mean : 16.67) than ruptured (9.78). 3. The correlation between serum and cul-de-sac β-hCG significant (r= 0.77, P $lt; 0.05) in ruptured group, and not significant (r= 0.46, P= 0.09) in unruptured. In all ectopic pregnancies, the correlation was significant (r= 0.57, P $lt; 0.05).