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윤길중,변상용,권효식,Yoon, Kil Joong,Pyun, Sang Yong,Kwon, Hyo Shik 대한화학회 1997 대한화학회지 Vol.41 No.7
과산화수소 정량을 위하여 닭의 간조직과 ferrocene을 탄소반죽에 혼입시켜 만든 전류법 바이오센서의 감응 특성이 조사되었다. 또한 조사된 전극 퍼텐샬 범위( - 0.5∼ + 0.05 V vs Ag/AgCl) 내에서 $t_{95%}$=13 sec의 빠른 감응시간과 $5.1{\times}10^{-5}$ M(S/N=3)의 검출한계를 보여 주었으며, 감응에 방해가 된다고 여겨지는 물질들에 대하여 좋은 선택성을 보여 주었다. 생촉매 안정성이 좋았으며, 이동매체를 사용하지 않는 전극의 제조 가능성을 제시하였다. The response characteristics of a new biosensor, which was developed by co-immobilisation of chicken liver tissue and ferrocene in a carbon paste matrix for the amperometric determination of hydrogen peroxide, was evaluated. In the range of electrode potential observed ( - 0.5∼ + 0.05 V vs Ag/AgCl), the bioelectrode showed the response time ($t_{95%}$) as low as 13 sec and the detection limit of $5.1{\times}10^{-5}$ M, and exhibited a good selectivity in the case of the addition of the possible interferents tested. Also it offered a high biocatalytic stability and opened up the possibility for the construction of a mediatorless biosensor.
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Rose Bengal 감응 광전류에 미치는 Urea의 영향
윤길중,강성철,김강진,Yun, Gil Jung,Gang, Seong Cheol,Kim, Gang Jin 대한화학회 1990 대한화학회지 Vol.34 No.6
광전기화학쎌, ITO/SnO$2$/rose bengal, NaClO$4$/Pt을 이용하여 들뜬 rose bengal로부터 박막 반도체 SnO$2$의 전도띠로 주입되는 전류의 크기를 조사하였다. 초감응제로써 urea를 첨가하여 광조사 시간의 경과에 따라 광전류는 증가하다가 일정시간 후에는 계속 감소하는 특성을 나타내었다. Urea를 포함하고 있는 염료용액을 분광학적인 방법으로 분석한 결과, 광화학 반응으로 염료의 농도는 시간에 따라 감소하지만 염료회합(dye aggregation)이 전류의 증가와 관련이 있는 것으로 나타났다. Electron injection from excited rose bengal into the conduction band of a thin film of SnO$2$, semiconductor was investigated in an electrochemical cell, ITO/SnO$2$/rose bengal, NaClO$4$/Pt. It was observed that urea enhanced the supersensitized photocurrent, followed by the reduction in the photocurrent after a shallow maximum. Spectroscopic analyses of the dye solution containing urea revealed that dye aggregation appeared to be involved in the increased photocurrent, although the concentration of the dye was continuously decreased dye to photochemical reactions.
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Electrochemical Evaluation of a Practical Carbon Paste Electrode to Determine Hydrogen Peroxide
윤길중 한국과학수사학회 2013 과학수사학회지 Vol.7 No.3
Styrene butadiene rubber solution for a binder of carbon powder and the marketed peroxidase extracted from horseradish for the biocatalyst were used to construct a biosensor to determine hydrogen peroxide. The symmetry factor (0.2), the exchange current density (2.42 × 10−5 A cm−2) and 10 and more electrode parameters were obtained for the cathodic branch of the signal current. They showed that the electrode fulfills its function normally and is not contrary to our expectations.
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윤길중 청주대학교 산업과학연구소 2006 産業科學硏究 Vol.24 No.1
An enzyme electrode for the determination of hydrogen peroxide was developed by immobilization of mugwort tissue in a carbon paste, and the effect of pH on ell catalytic power was investigated. Current profile of the biolenlor well pH slowed two peaks, which mean that mugwort contains two kinds of peroxide. Assuming that the reduction function of peroxide is Gaullist distribution std pH-dependent current profile is the sum of two Gosling, current profiles were resolved into two galloons at various monitoring concentrations of substrate. This enabled to determine the optimum pH of each ilozyme.
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Rose Bengal의 광촉매 반응에 의한 $I_2$의 생성
윤길중,Yoon, Kil-Joong 한국분석과학회 1996 분석과학 Vol.9 No.1
감응제 rose bengal과 초감응제 $I^-$를 포함하는 광전기화학쎌을 이용하여 태양에너지를 전기적 에너지로 전환할 때 광전류는 안정화되고 지속적이지만 장시간의 광조사시에는 서서히 감소한다. 염료용액을 분광학적 및 전기화학적으로 분석한 결과 rose bengal과 $I^-$의 광촉매 반응에 의하여 $I_2$가 생성되고 $I_2$가 빛이 없는 조건하에서도 rose bengal과 반응하여 염료 농도를 감소 시키는것이 확인되었다. In the process of solar energy conversion into electrical energy using the photoelectrochemical cell containing the sensitizer, rose bengal and supersensitizer. $I^-$, the photocurrent is stabilized and durable. But the long time span of irradiation causes the decrease of photocurrent monotonically. Spectroscopic and electrochemical analyses of rose bengal solution containing $I^-$ revealed that the decrease of concentration of rose bengal was attributed to the reaction of rose bengal in the dark with $I_2$ formed as a result of the possible photocatalytic reaction of rose bengal with $I^-$.
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Qualitative Analysis of a Natural Rubber-Bound Biosensor to Determine Hydrogen Peroxide
윤길중 청주대학교 2013 産業科學硏究 Vol.31 No.1
흑연가루 결합재로 천연고무를, 생촉매로 고추냉이 과산화효소를 사용하여 과산화수소 정량 바이오센서를 제작하였다. 바이오센서 제작 후 톨루엔의 빠른 증발로 인하여 탄소반죽 전극은 탁월한 기계적 물성을 얻을 수 있었으며, 이것은 전극의 실용 및 반영구적 이용을 가능케 했다. 신호전류의 환원 영역에서 얻은 전극의 대칭인자(α, 0.2), 교환전류(io, 1.0x10-5 A) 및 기타 10여 개 이상의 전극 파라메터는 전극이 정상적으로 작동하고 있으며 천연고무가 탄소가루의 결합제로 활용될 수 있음을 보여 주었다.
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에틸렌-프로필렌 결합재를 이용한 과산화수소 감응 탄소반죽 바이오센서의 속성 제작
윤길중 청주대학교 2016 産業科學硏究 Vol.33 No.2
The biosensor that HRP was incorporated in carbon paste bound with EPDM rubber, a bonding agent of carbon powder, was constructed and its behaviors for the sensibleness of hydrogen peroxide were evaluated electrochemically. A good linearity of Tafel plot and more than ten electrochemical parameters indicated that the enzyme electrode fulfills its function of the sensor for the determination of hydrogen peroxide quantitatively. And the small value of symmetry factor(α, 0.20) demonstrated the ratio of the change of the peak height of activation energy to that of electrode potential has a steep gradient. The experimental facts collected in this work suggested that EPDM, which is a cheap product of Ziegler-Natta reaction, can be used in the place of the mineral oil.
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부타디엔 고무로 결합된 탄소반죽 효소고정 과산화수소 바이오센서의 제작
윤길중 청주대학교 2017 産業科學硏究 Vol.35 No.1
An enzyme electrode bound by BDR dissolved in toluene, which is specific to hydrogen peroxide, was newly designed and its electrochemical properties were appraised in the potentiostatic methodology. The electrode showed a fast signal appearance and the electrode-fed electrochemical parameters demonstrated that it functions qualitatively and quantitatively. Besides, volatilization of toluene immediately after electrode manufacturing gave a mechanical robustness to electrode material and this met the qualifications of the enzyme electrode. Those above gave us a brilliant prospect for the actual use and for the mass production of the carbon paste electrode.
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