해수에 잠긴 인공기질 표면에 먼저 부착된 박테리아가 규조류의 부착에 미치는 영향

강정훈,조병철,심재형,최중기,Kang, Jung-Hoon,Cho, Byung-Cheol,Shim, Jae-Hyung,Choi, Joong-Ki 한국해양학회 1998 바다 Vol.3 No.4

해수에 잠긴 인공기질 표면에 부착한 박테리아가 규조류의 후속부착에 미치는 영향에 대해 상반된 견해가 있다. 그러나 자연조건의 해수에서 인공기질 표면에 먼저 부착한 해산 박테리아가 규조류의 후속부착에 어떤 영향을 주는지에 대해서는 아직 알려져 있지 않다. 따라서 본 연구에서는 해산 박테리아를 일정 시간별로 미리 부착시킨 여러 재질을 이용하여 실험하였다. 첫번째 실험에서는 유리 슬라이드에 미리 해산 박테리아를 0~133 시간별로 부착시키고 해수에 일정기간 노출시킨 후 규조류의 부착수도를 조사하였다. 규조류의 부착은 먼저 부착된 박테리아의 수도와 관련이 없었으며(p>0.05), 무처리 슬라이드에 가장 많은 수의 규조류가, 가장 높은 수도의 박테리아가 부착된 표면에는 가장 적은 수의 규조류가 부착하였다. 두번째 실험에서는 유리와 아크릴 슬라이드를 이용하여 해산 박테리아를 6 일 동안 먼저 부착시킨 것과 한천(agar)을 입힌 슬라이드 그리고 대조구로서 무처리 슬라이드를 해수 중에 함께 노출시켰다. 박테리아가 가장 높은 수도($5.4{\pm}0.02{\times}10^5\;cells\;cm^{-2}$)로 부착된 아크릴 슬라이드에서는 무처리 슬라이드보다 적은 수의 규조류가 부착하였으나, 박테리아가 $2.5{\pm}1.0{\times}10^5\;cells\;cm^{-2}$로 부착된 유리 슬라이드에서는 무처리 슬라이드와 차이가 없었다. 반면 점액질을 모사한 표면에 부착한 규조류의 수도와 이입계수는 다른 표면들에 비해 평균 2 배 이상 높게 나타났다. 따라서 해산 박테리아의 부착이 규조류 후속부착의 선결요건이 아닌 것으로 판단된다. Controversial observations on the effect of pre-attached bacteria on the attachment of diatoms to artificial surfaces immersed in seawater have been made. Furthermore, it is not known whether or not pre-attached bacteria on artificial surfaces influence attachment of diatoms in natural seawater. In this study, we used various surfaces to which marine bacteria were pre-attached for different incubation periods. In the first experiment, glass slides were initially attached by marine natural bacteria with different exposure time (0-133 hr) and then immersed into seawater for a certain time period. The attachment of diatoms was not affected by the abundance of pre-attached bacteria (p > 0.05). The maximum abundance of attached diatoms was found on control surfaces, and the minimum abundance on surfaces where attached bacterial abundance was highest. In the second experiment, glass slides and acryl slides, either attached by marine natural bacteria for 6 days or coated by agar, were immersed in seawater. Untreated slides were also employed. On the surfaces of acryl slides with the most abundant attached bacteria ($5.4{\pm}0.02{\times}10^5\;cells\;cm^{-2}$), abundances of attached diatoms were less than those on untreated slides. On the surfaces of glass slides with bacterial abundance of $2.5{\pm}1.0{\times}10^5\;cells\;cm^{-2}$, however, abundances of attached diatoms were not different from those of untreated slides. On the agar-coated slides, the immigration rate and immigration coefficient were on average > 2 folds compared to other surfaces, indicating high rates of diatom attachment on mucilage simulated surfaces. Therefore, it seems that pre-attachment of bacteria is not prerequisite for the attachment of diatoms on artificial surfaces.

춘계 독도 주변해역 야광충의 먹이생물

강정훈,KANG, JUNG-HOON 한국해양학회 2020 바다 Vol.25 No.4

To understand on-site feeding traits of Noctiluca scintillans with unprecedented high abundances around Dokdo in spring, 2014, the composition and abundance of food items in the digestive vacuole of the species were analyzed. Abundances of N. scintillans ranged from 4,328~17,791 inds.m^-3 around Dokdo during the study. The incidence of preyed items in the vacuole of N. scintillans ranged by averaged 32% (24~50%) in the surface waters around Dokdo. Diverse preyed items consisted of fecal pellet, protozoans, pollen, diatoms, dinoflagellates, copepods and ichtyoplankton, while fecal pellets were dominantly fed by N. scintillans (43%) and next by protozoans (19%), pollen (18%), diatoms (7%) and dinoflagellates (4%). Fecal pellet, protozoans and pollen were relatively preferred by N. scintillans compared to diatoms, dinoflagellates and ichtyoplankton based on the incidence in the food vacuoles. Present results indicated that high abundance of N. scintillans found around Dokdo was likely associated with non-phytoplankton items rather than previously well-known diatoms as prey during the study.

유방염 치료후 휴약기간이 지난 원유내 잔류약제에 관한 연구

강정훈,김진석,이원창,Kang, Jeong-hun,Kim, Jin-suk,Lee, Won-chang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

From July of 1997 to June of 1998, total 279 raw milk samples over withdrawal period after mastitis treatment from dairy farms located in the provinces of Kyonggi and Choongchung were collected to test drug residues. Each sample was tested by TTC- II test and Delvotest SP. Among the total 152 raw milk samples of cow treated by ${\beta}$-lactams, 32 samples(21.2%) were positive on the Delvotest and 15 samples(9.9%) showed positive on the TTC-II test. Also, from the total 37 samples treated by sulfonamides, 5 samples(13.5%) were positive on the Delvotest and 3 samples(8.1%) showed positive on the TTC-II test. For the total 55 raw milk samples of cow treated by tetracyclines, 9 samples(16.4%) were positive on the Delvotest and 5 samples(9.1%) showed positive on the TTC-II test. In addition, from the total 35 samples treated by aminoglycosides, 7 samples(20.0%) were positive on the Delvotest and 5 samples(14.3%) showed positive on the TTC-II test. Our study shows that it is possible that drugs are to be detected by the drug residues test of an individual raw milk even over the withdrawal period after mastitis treatment and the raw milk of bulk tank.

원유내 약물잔류의 원인에 관한 연구

강정훈,김진석,최필수,이원창,Kang, Jeong-hun,Kim, Jin-suk,Choi, Pill-su,Lee, Won-chang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6

The 102 farms received a positive result of the bulk milk drug residue test were selected to investigate the reasons of drug residues in bulls milk. The most frequent causes of drug residues were milker or producer mistakes (28.4%), failure to observe withdrawal time (21.5%), and withholding milk from treated quarters only (19.6%). Milker or producer mistakes occurred high at the farms having a parlor system (4 cases out of 11 farms), and related to the inadequate records and marking of treated cows. The lack of knowledge on the absorption of antibiotic from treated quarters and its excretion from untreated quarters caused mainly withholding milk from treated quarters only. Among the 91 farms identified the cause of drug residues, most of the route of drug administration was intramammary infusion (81.3%), and mostly drug used for the treatment of cows was ${\beta}$-lactam antibiotic (57.1%)

대두 ( Glycine max ) 로 부터 Diamine Oxidase 의 정제 및 특성

강정훈,조영동 ( Jung Hoo Kang,Young Dong Cho ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.3

Diamine oxidase (EC 1.4.3.6) present in extracts of axes of soybean (Glycine max) seedling has been purified to homogeneity using series of ammonium sulfate fractionation, DEAE-cellulose, phosphocellulose, hydroxyapatite and 1,12-diaminododecane agarose column chromatography. The molecular weight estimated by a Sephadex G-200 gel filtration was about 145,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecular weight of 70,000. The optimum pH for activity was 7.5. Putrescine was the best substrate, and spermidine and spermine also served as substrates in decreasing order. The K_m values for putrescine, spermidine and spermine were 5.3×10^(-4)M, 2.8×10^(-5)M and 6.5×10^(-5)M, respectively.

원발성 및 전이성 구강편평세포암종 세포주에서 p21 및 p73 mRNA발현에 관한 연구

강정훈,김경욱,이재훈,Kang, Jeong-Hoon,Kim, Kyung-Wook,Lee, Jae-Hoon 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.6

There were many controversies in the cause and progress of tumorigenesis. Recently, studies on the mutation of genes related to the tumor have extensively been performed due to development of molecular biology. Structural and morphological changes of chromosomes, which are related to the abnormal activation of oncogenes or inactivation of tumor suppression genes, transform the normal cells into the tumor cells. p53 and Rb are well known tumor suppressor genes, while oncogenes include c-myc, bcl-2 and ras, etc. When exposed to cell damaging agents, p53 inhibits cell growth by inducing transcription of p21. Especially p73, which is homo-logy of p53, frequently deleted in melanoma, neuroblastoma, colon cancer, and breast cancer, when over produced, p73 activates the transcription of p21, bax-1 and inhibits cell growth by inducing apoptosis. For study on mRNA expression of p21 and p73, normal oral keratinocytes, and cell lines of primary and metastatic oral squamous cell carcinoma were cultured and then electrophoresis and RT-PCR(reverse transcription-polymerase chain reaction) were performed. 1. The mRNA of p21 and p73 in normal oral keratinocyte expressed lower than that of primary squamous cell carcinoma. 2. The mRNA of p21 in metastatic oral squamous carcinoma cell lines was expressed as various patterns compared with that of normal oral keratinocyte. 3. In the metastatic oral squamous cell lines, the mRNA of HN8 expressed higher than that of HN12 or HN19. 4. The mRNA of p73 in primary oral squamous cell lines expressed 4-5 times higher than that of normal keratinocyte. 5. In metastatic oral squamous cell lines, there was no significant expression of p73 mRNA compared with that of normal oral keratinocyte. From the results obtained in this study, mRNA expression of p73 in primary oral squamous cell lines was remarkable, while mRNA expression of p21 and p73 in metastatic oral squamous cell lines were statistically insignificant.

Purification and Properties of Diamine Oxidae from Soygean(Glycine max)

강정훈,조영동,Kang, Jung-Hoon,Cho, Young-Dong 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.3

대두(Glycine max)축에 존재하는 diamine oxidase (EC 1.4.3.6)를 $(NH_4)_2SO_4$ 침전법, DEAE-cellulose, phosphocellulose, hydroxyapatite, affinity chromatography 등을 이용하여 정제하였다. 정제된 효소의 분자량은 Sephadex G-200 gel filtration에 의해 145,000으로 판명되었다. 정제된 효소는 SDS-PAGE 상에서 단일 띠를 나타냈다. 효소활성에 대한 최적 pH는 7.5였다. Putrescine은 이 효소에 대해 가장 좋은 기질이었으며 spermidine과 spermine도 기질로 작용하였다. Putrescine, spermidine, spermine에 대한 효소의 Km 값은 각각 $5.3{\times}10^{-6}M$, $2.8{\times}10^{-5}M$, $6.5{\times}10^{-5}$이었다. Diamine oxidase (EC 1.4.3.6) present in extracts of axes of soybean (Glycine max) seedling has been purified to homogeneity using series of ammonium sulfate fractionation, DEAE-cellulose, phosphocellulose, hydroxyapatite and 1,12-diaminododecane agarose column chromatography. The molecular weight estimated by a Sephadex G-200 gel filtration was about 145,000. Sodium dodecyl sulfate polyacrylamide gel electrophoresis gave a single band at the molecular weight of 70,000. The optimum pH for activity was 7.5. Putrescine was the best substrate, and spermidine and spermine also served as substrates in decreasing order. The $K_m$ values for putrescine, spermidine and spermine were $5.3{\times}10^{-4}M$, $2.8{\times}10^{-5}M$ and $6.5{\times}10^5M$, respectively.

가속도계를 이용한 광전용적맥파의 동잡음 제거

강정훈,조백환,이종실,지영준,김인영,김선일,Kang, Joung-Hoon,Cho, Baek-Hwan,Lee, Jong-Shill,Chee, Young-Joon,Kim, In-Young,Kim, Sun-I. 대한의용생체공학회 2007 의공학회지 Vol.28 No.3

With the convergence of ubiquitous networking and medical technologies, ubiquitous healthcare(U-Healthcare) service has come in our life, which enables a patient to receive medical services at anytime and anywhere. In the u-Healthcare environment, intelligent real-time biosignal aquisition/analysis techniques are inevitable. In this study, we propose a motion artifact cancelation method in portable photoplethysmography(PPG) signal aquisition using an accelerometer and an adaptive filter. A preliminary experiment represented that the component of the pedestrian motion artifact can be found under 5Hz in the spectral analysis. Therefore, we collected PPG signals under both simulated conditions with a motor that generates circular motion with uniform velocity (from 1 to 5Hz) and a real walking condition. We then reduced the motion artifact using a recursive least square adaptive filter which takes the accelerometer output as a noise reference. The results showed that the adaptive filter can remove the motion artifact effectively and recover peak points in PPG signals, which represents our method can be useful to detect heart rate in real walking condition.

Longitudinal Distribution of Zooplankton at 10.5^°N in the Northeastern Pacific

강정훈,조규희,손주원,김웅서,Kang, Jung-Hoon,Cho, Kyu-Hee,Son, Ju-Won,Kim, Woong-Seo Korea Institute of Ocean Science Technology 2007 Ocean and Polar Research Vol.29 No.4

We investigated the longitudinal variations in zooplankton abundances and their related physicochemical properties at nine stations located between $136^{\circ}W$ and $128^{\circ}W$ at $10.5^{\circ}N$ in the northeastern Pacific in summer 2004. Temperature, salinity, inorganic nutrients, chlorophyll-a (hereafter chl-a) and zooplankton ($>200\;{\mu}m$) were sampled within the depth from the surface to 200 m depth at $1^{\circ}$ longitude intervals. Zooplankton($>200\;{\mu}m$) samples were vertically collected at two depth intervals from surface to 200 m, consisting of surface mixed and lower layers (thermocline$\sim$200 m). Longitudinal distributional pattern of hydrological parameters (especially salinity) was physically influenced by the intensity of westward geostrophic current passage relating to the NEC (North Equatorial Current). Data from the longitudinal survey showed clear zonal distributions in the hydrological parameters(temperature, salinity and nutrients). However, spatial patterns of the chl-a concentrations and zooplankton abundances were mostly independent of the zonal distributions of hydrological parameters. The two peaks of zooplankton abundance in the surface mixed layer were characterized by different controlling factors such as bottom-up control from nutrients to zooplankton ($129^{\circ}W$) and accumulation by increment of friction force and taxonomic interrelationship ($133^{\circ}$ and $134^{\circ}W$). Divergence-related upwelling caused introduction of nutrients into surface waters leading to the increment of chl-a concentration and zooplankton abundances ($129^{\circ}W$). Increased friction force in relation to reduced flow rates of geostrophic currents caused accumulation of zooplankton drifting from eastern stations of study area($133^{\circ}$ and $134^{\circ}W$). Besides, high correlation between immature copepods and carnivorous groups such as chaetognaths and cyclopoids also possibly contributed to the enhanced total abundance of zooplankton in the surface mixed layer (p<0.05). Zooplankton community was divided into three groups (A, B, C) which consecutively included the eastern peak of zooplankton($129^{\circ}W$), the western peak($133^{\circ}$ and $134^{\circ}W$) and high nutrient but low chl-a concentration and zooplankton abundance ($136^{\circ}W$). Moreover, Group B corresponded to the westward movement of low saline waters(<33.6 psu) from 128 to $132^{\circ}W$. In summary, longitudinal distributions of zooplankton community was characterized by three different controlling factors: bottom-up control ($129^{\circ}W$), accumulation by increased friction force and relationships among zooplankton groups ($133^{\circ}$ and $134^{\circ}W$), and mismatch between hydrological parameters and zooplankton in the high nutrient low chlorophyll area ($136^{\circ}W$) during the study period.

In-memory DB queue를 이용한 IoT 빅데이터 처리 SW

강정훈 ( Jeonghoon Kang ),채철승 ( Chulseoungchae ),김형구 ( Hyeonggoo Kim ),민수영,이명수,박부식,이상엽 한국정보처리학회 2019 한국정보처리학회 학술대회논문집 Vol.26 No.2

본 논문은 손실이 큰 IoT 빅데이터 처리 고속화 SW의 Queue를 In-memory DataBase로 이용하여 전처리 프레임워크 기술에 대하여 제안하였다.