소화효소가 Helicobacter pylori의 증식과 공포화독소 생성에 미치는 영향

홍대식,강경희,장명웅 고신대학교(의대) 고신대학교 의과대학 학술지 2001 고신대학교 의과대학 학술지 Vol.16 No.1

Background : Global high prevalence rate of Helicobacter pylori infection is well-known fact. However, a large discrepancies exist between the diagnostic data obtained by the PCR and culture method, because the coccoid form of H.pylori which represent the majority form in the biological samples are not viable in the standard BHI broth with 5% horse serum. The component parts of saliva and gastric juice acted as neither stimulator nor inhibitor to the growth of H.pylori remain unclear. Objective : The purpose of this study was to evaluate the influence of amylase, pepsin, mucin, and lysozyme on the growth and vacuolating toxin production and titer of H.pylori in vitro. Materials and Methods : H.pylori 13,39,46,59,72,94,98 and 125 strains were isolated from patients with gastric disease. The digestive enzymes were inoculated in the 5% horse serum-BHI broth following final concentration of amylase (A; 0.20ug/ml), pepsin (P; 0.25ug/ml), mucin (M; 2,5ug/ml), and lysozyme (L; 1.5ug/ml). The vacuolating toxin titers of the culture supernatant were evaluated in the monolayer culture of RK-13 cells. Results : The morphologic conversion from bacillary to horse-shoe to coccoid forms of H.pylori was observed in the BHI broth containing 5% horse serum on day 5 culture. Half of H.pylori was changed from bacillary to coccoidal form on day 7 culture, most population of H.pylori was changed to coccoidal form on day 10 culture, and almost all H.pylori was changed to coccoidal form on day 14 culture. The growth of H.pylori 72, and 94 strains was increased in BHI broth containing each enzyme such as amylase, pepsin, mucin and mixture of two or three enzymes on day 5 and 7 culture. The growth of H.pylori 94 strains was increased in BHI broth containing each enzyme such as amylase, pepsin, mucin, and mixture of two or three enzymes on day 10 and 14 culture. The vacuolating toxin production of H.pylori strains was not influenced by the addition of amylase, pepsin, mucin, and lysozye or mixture of two or three enzymes. The neutralization of vacuolating toxin of H.pylori was not affected by the addition of amylase, pepsin, mucin, and lysozyme or mixture of two or three enzymes. Conclusion : We found that coccoid forms of H.pylori were viable in the standard 5% horse serum BHI media in the presence of amylase, pepsin, mucin, and lysozyme. The present study demonstrates that gastric juice of the stomach supports the viability of H.pylori and that oral route of infection is a plausible hypothesis. This work also demonstrates that gastric environment may not provide a selective advantage for the toxigenic strains of H.pylori.

상명대학교 공과대학 수학교육 강화방안 연구

홍대기(Hong, Dae-Ki),조태경(Cho Tae-kyung),박병수(Park Byoung-soo) 한국산학기술학회 2008 한국산학기술학회논문지 Vol.9 No.5

최근 국내대학 전반에 걸쳐서 공과대학의 우수 지원자가 점차로 감소되고 있는 상황이며 고등학교 수학 교육과정의 부실화에 따른 신입생의 학력저하에 대한 우려 또한 제기되고 있다. 이러한 위기상황에 대응하여 상명대학교에서는 공학교육의 발전 및 혁신을 위해 공학교육 혁신센터를 운영하고 있다. 공학교육 혁신센터는 공학교육 혁신을 위한 장가계획의 일환으로 수학 분야의 교육 강화를 위한 여러 가지 방안을 논의하여 노출하고 있다. 본 논문에서는 상명대학교에서 논의하여 도출한 수학 교육 강화방안을 제시한다. 이러한 결과들은 상명대학교 공과대학과 유사한 환경에 처해있는 다른 학교들의 수학 교육 강화를 위한 참고자료로 활용될 수 있을 것이다. Recently, the number of applicants for the engineering college is gradually decreased all over the nation. Additionally, the concerns of fall of freshman mathematical ability are raised due to the incomplete high school education. Therefore, the local universities endeavor in order to improve the quality of mathematics education, and study the countermeasure against the crisis. As part of these efforts, Sang-Myung University founds the innovation center for engineering education. The center derives several ideas in order to enhance the mathematics education quality. In this paper, the enhancement plans for the mathematics education in Sang-Myung University are proposed. Results in this paper will be helpful for other university to enhance the mathematics education as reference materials.

전문상담교사제도의 오늘과 내일

홍대우 한국카운슬러협회 2010 相談과 指導 Vol.45 No.-

2005년 9월 1일 전국 182개 지역교육청에 308명의 전문상담(순회)교사가 배치(실제는 미달하여 2006년 3월에 충원됨)된 이후, 2010년 3월 현재 926명의 전문상담(순회)교사가 배치되어 있다. 전문상담교사제도는 우리나라 학교상담발전 뿐만 아니라 청소년 문제예방 및 상담치료 그리고 학교교육의 패러다임을 변화시키는 계기가 됨과 아울러 청소년을 지역사회와 함께 지도하고자하는 지역사회 교육공동체 운동으로서 큰 변화를 가져오는 계기가 되었다. 아직은 제도 초기여서 제대로 정착되기 위해서는 더 많은 노력이 필요하며, 시련과 아픔들을 겪어야 하리라 예상된다. 여기서는 전문상담교사제도와 관련된 그 동안의 중요한 내용들을 짚어 보고 제도의 발전 방안을 함께 생각해보고자 한다.

중국 복건성 객가족의 토루주택에 관한 연구

홍대형,최장순 대한건축학회 1998 대한건축학회논문집 Vol.14 No.10

ADAS to Automated Driving Systems Level 3

홍대건(Dae-Gun Hong) 한국자동차공학회 2018 한국자동차공학회 Workshop Vol.2018 No.7

Corporate Entrepreneurship 활성화 방안 연구: 국내 대기업의 사내벤처제도 사례를 중심으로

홍대순,이정훈,이혜원 기술경영경제학회 2014 Journal of Technology Innovation Vol.22 No.3

최근, 기존 주력산업의 안주, 혁신 기피 현상으로 말미암아 기업 내에서 지속적인 성장 동력에 대한 이슈가 발생하고 있다. 이러한 지속성장의 돌파구로써 새롭게 주목 받고 있는 대안이 바로 사내기업가정신(Corporate Entrepreneurship)이다. 혁신을 촉진시키는 효과적 방안으로 사내기업가정신이 주목받음에 따라, 국내 기업들 사이에서는 사내벤처 형태의 사내기업가 정신 촉진 활동을 통해 혁신적인 신제품/ 신사업을 발굴하며, 계층적이고 경직된 조직문화를 지양하고, 보다 유연하고 혁신적인 조직문화를 정착시키는 시도를 해오고 있다. 본 연구에서는 사내벤처를 운영하는 3개의 국내 기업을 대상으로 사례 연구를 실시하였으며, 사례연구에 있어서는 사내기업가정신 발현유형과 사내기업가정신 활성화 프레임워크를 결합하여 사례를 분석함으로써, 사내기업가정신 발현유형에 따라 사내기업가정신 활성화 제도의 지향점, 운영 등에 있어서 차이가 있음을 규명 하였다. Recently, there have been an increasing concern for a continuous growth inside the company due to its being complacent about their present business and not taking challenges for innovation. Corporate entrepreneurship is the one that receives an attention as a breakthrough for this growth. Since it has been perceived as an effective means of promoting an innovation, Korean firms have tried to develop innovative products and businesses, reject hierarchical and rigid organizational culture, and settle more flexible and innovative culture through corporate venturing system. In this research, case studies on three Korean firms’corporate venturing system were conducted. It also investigated differences in operation and vitalization of corporate venturing system depending on the type of how it is implemented in the company by analyzing case studies with combining the type of corporate entrepreneursip's realization and the framework for its vitalization.

공정거래법상 과징금 제도의 현황과 개선방안

홍대식(Hong Dae sik) 행정법이론실무학회 2007 행정법연구 Vol.- No.18

The administrative fine system in the Monopoly Regulation and Fair Trade Act of Korea(the “Act“) seeks to deter the violator and other undertakings in the same market from repeating the same or similar types of infringements. Its validity from the functional perspective is supported by way of economic explanation with the theory and analysis model. However, for the difficulty and unreality of finding out the economically optimal level of deterrence in every specific case, it is necessary to develop the notion of proxy on which a normative approach is based. The current system adopts relevant turnover-based principle and step-by-step methodology which undergoes a calculation of the basic administrative fine stage, an obligatory adjusting stage, a discretionary adjusting stage, and finally a determination of the administrative fine stage. The problem is that the calculation of administrative fines following the arithmetical rules contained in the Korea Fair Trade Commission(the “KFTC“)'s guidelines does not guarantee the result which corresponds with the legal principles such as those of proportionality and equal treatment. It arises not only from the KFTC's practice which largely depends on the internal guidance with insufficient grounds and data but from the guidelines's flaw which lacks the process enabling the administrative fine to take balance with the scale of improper gains as a statutorily obligatory consideration factor. This article suggests several practical ways including amendments to the guidelines by which the administrative fine system should be improved so as to enhance transparency and predictability and to promote consistency with the legal framework. This will help to relieve making mistakes of 'missing the wood for the trees' in determining the administrative fine.

PCS 방식별 성능 비교

홍대형 한국통신학회 1995 한국통신학회 워크샵 Vol.1995 No.8

미국 의회와 FTA정책: 조지 W. 부시 행정부의 사례(2001-06)

홍대운 ( Dae Un Hong ) 서울대학교 미국학연구소 2008 미국학 Vol.31 No.2

연재 - 우리 건축문화재 다시보기

홍대형,Hong, Dae-Hyung 대한건축사협회 2010 建築士 Vol.2010 No.4