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Growth Promoting Activity of a Macromolecular Fraction from Fresh Ginseng
Eun, Song-Myung,Hung, Ng-Kam,Nam, Leung-Kwok,Cheung, Kong-Yum The Korean Society of Ginseng 1989 Journal of Ginseng Research Vol.13 No.2
A macromolecular fraction from fresh ginseng root containing mainly polysaccharide showed mild mitogenic activity in human cord blood lymphocytes. 2) When lymphocytes were transformed by Con A or PHA, this fraction could greatly enhance the activity of these lectin mitogens, thus showing a potentiation effect. 3) Although this macromolerular fraction contains saponin and is susceptible to trypsin digestion, it is probably a peptido-glycan in nature on account of its important carbohydrate content and thermal stability. 4) This fraction could not support cancer cell (EAT, K562) growth : its inhibitory effect on these cells remains to be explored.
Partial Purification and Characterization of a Glycoprotein Factor from Fresh Ginseng
Kong, Yun-Cheung,Fong, Wing-Ping,Song, Myung-Eun,Ng, Kam-Hung,Ho, Dan-Dan,Ng, Ping-Chung The Korean Society of Ginseng 1990 Journal of Ginseng Research Vol.14 No.2
The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and co-mitogenic activities in human peripheral blood lymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose, DEAE-Sepharose) yielded a semi.purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in lymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.