Experimental assessment of slamming coefficients for subsea equipment installations

de Oliveira, Allan C,Pestana, Rafael G Techno-Press 2020 Ocean systems engineering Vol.10 No.2

Considering the huge demand of several types of subsea equipment, as Christmas Trees, PLEMs (Pipeline End Manifolds), PLETs (Pipeline End Terminations) and manifolds for instance, a critical phase is its installation, especially when the equipment goes down through the water, crossing the splash zone. In this phase, the equipment is subject to slamming loads, which can induce impulsive loads in the installation wires and lead to their rupture. Slamming loads assessment formulation can be found in many references, like the Recommended Practice RP-N103 from DNV-GL (2011), a useful guide to evaluate installation loads. Regarding to the slamming loads, RP-N103 adopt some simplifying assumptions, as considering small dimensions for the equipment in relation to wave length, in order to estimate the slamming coefficient C_S used in load estimation. In this article, an experimental investigation based on typical subsea structure dimensions was performed to assess the slamming coefficient evaluation, considering a more specific scenario in terms of application, and some reduction of the slamming coefficient is achieved for higher velocities, with positive impact on operability.

Processing and Assembly in vitro of Engineered Soybean b-Conglycinin Subunits with the Asparagine-Glycine Proteolytic Cleavage Site of 11S Globulins

Luiz O. Oliveira,남영우,Rudolf Jung,Niels C. Nielsen 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.1

A short interdomain sequence between the N- and Cterminal domains of b-conglycinin, the major 7S seed storage protein of soybean, was selected as a target for insertion of amino acid residues specifically cleaved by an asparaginyl endopeptidase that processes 11S globulins into acidic and basic chains. Modified b- conglycinin subunits containing the proteolytic cleavage site self-assembled into trimers in vitro at an efficiency similar to that of the unmodified subunit. In contrast to the absence of cleavage of the unmodified subunits, however, the modified b-conglycinin trimers were processed by purified soybean asparaginyl endopeptidase into two polypeptides, each the size expected for the b-conglycinin N- and C-terminal domains, respectively. The cleavage did not alter the assembly of mutant b-conglycinins and the cleaved mutant trimers remained stable to further proteolytic attack. To examine the possibility of coassembly between the cleaved 11S and 7S subunits, in vitro processed mutant b-conglycinin subunits were mixed with native dissociated 11S globulin preparations. Reassembly at a high ionic condition did not induce the 7S subunits to interact with 11S subunits to form hexameric complexes. Thus, cleavage of 7S globulin subunits into acidic and basic domains may not be sufficient for hexamer as-

Does Azospirillum brasilense and biostimulant improve the initial growth of rice sown at greater depths?

Carlos Eduardo da Silva Oliveira,Tiago Zoz,Eduardo Pradi Vendruscolo,Agner de Freitas Andrade,Cássio de Castro Seron,Travis Wilson Witt 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.5

The sowing operation is crucial for the proper initial establishment of seedlings and their development. However, there is a lack of studies about the eff ects of sowing depth of rice, as well as technologies that aid to mitigate the prejudicial eff ects caused by depth. In this way, the present work aimed to evaluate the initial growth of rice plants inoculated with Azospirillum brasiliense and treated with biostimulant sowed at diff erent depths. Randomized complete block design with four replica�tions was used, in a 4 × 4 factorial scheme. The fi rst factor was composed of diff erent seed treatments (a, control—with�out treatment; b, inoculation with A. brasilense ; c, seed treatment with Stimulate® and d, inoculation with A. brasilense associated with seed treatment with Stimulate ® ). The second factor was composed of four sowing depths (1.0, 3.0, 5.0 and 7.0 cm). Treatment of rice seeds with A. brasilense and Stimulate® decreases the rate of emergence. The sowing at 7 cm depth decreases in 41.44% of the emergence, when compared to sowing at 1 cm. Inoculation with A. brasilense and sowing at increasing depths are prejudicial to the growth of rice crop.

Antidiabetic Effect of the Chrysobalanus icaco L. Aqueous Extract in Rats

Ana Paula de Oliveira Barbosa,Gabriela de Oliveira Silveira,Igor Alexandre Cortes de Menezes,Jose´ Melquiades Rezende Neto,Jose´ Leal C. Bitencurt,Charles dos Santos Estavam,Amanda do Carmo Bion de Li 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.6

Chrysobalanus icaco L. is a medicinal plant popularly known in Brazil as ‘‘Grageru’’ or ‘‘Abageru.’’ It is used in African and American continents as medicinal food in the treatment of several diseases, including diabetes. This study used phytochemical screening to determine the antioxidant and α-amylase inhibitor activities of the aqueous extract (AECI) of C. icaco, and evaluated its antidiabetic potential in rodents. Phytochemical screening was performed using colorimetric tests with specific reagents. The in vitro antioxidant activity was evaluated by the scavenging activity of 2,2-diphenyl-1-picril-hydrazyl. The lethality test and behavioral screening was performed using an oral administration of 5 g/kg of AECI. The antidiabetic potential of AECI was evaluated through the oral glucose tolerance test (OGTT) and chronic hypoglycemic test at the doses of 100, 200, and 400 mg/kg (orally). Metformin was used as a reference drug in all tests. Diabetes was induced by injection of alloxan (40 mg/kg; intravenously). Phytochemical screening showed the presence of various compounds, including tannins, flavones, triterpenoids, steroids, saponins, and alkaloids. The in vitro antioxidant test demonstrated that AECI presented potent antioxidant activity. The lethality test and behavioral screening did not show lethality signs. In the OGTT test, AECI administration was not able to inhibit the elevation of glycemia. However, chronically administrated, it was able to cause a significant (P < .05) reduction of glycemia from 335 ± 27 up to 197 ± 15 mg/dL. These results demonstrate that the AECI presents a potential beneficial effect for diabetes.

Meat Quality of Lambs Fed on Palm Kernel Meal, a By-product of Biodiesel Production

Ribeiro, R.D.X.,Oliveira, Ronaldo Lopes,Macome, F.M.,Bagaldo, A.R.,Silva, M.C.A.,Ribeiro, C.V.D.M.,Carvalho, G.G.P.,Lanna, D.P.D. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.10

This study aimed to establish the optimum level of palm kernel meal in the diet of Santa Ines lambs based on the sensorial characteristics and fatty acid profile of the meat. We used 32 lambs with a starting age of 4 to 6 months and mean weight of $22{\pm}2.75kg$, kept in individual stalls. The animals were fed with Tifton-85 hay and a concentrate mixed with 0.0, 6.5, 13.0 or 19.5% of palm kernel meal based on the dry mass of the complete diet. These levels formed the treatments. Confinement lasted 80 days and on the last day the animals were fasted and slaughtered. After slaughter, carcasses were weighed and sectioned longitudinally, along the median line, into two antimeres. Half-carcasses were then sliced between the 12th and 13th ribs to collect the loin (longissimus dorsi), which was used to determine the sensorial characteristics and fatty acid profile of the meat. For sensorial evaluation, samples of meat were given to 54 judges who evaluated the tenderness, juiciness, appearance, aroma and flavor of the meat using a hedonic scale. Fatty acids were determined by gas chromatography. The addition of palm kernel meal to the diet had no effect on the sensorial characteristics of meat juiciness, appearance, aroma or flavor. However, tenderness showed a quadratic relationship with the addition of the meal to the diet. The concentration of fatty acids C12:0, C14:0 and C16:0 increased with the addition of palm kernel meal, as did the sum of medium-chain fatty acids and the atherogenicity index. Up to of 19.5% of the diet of Santa Ines lambs can be made up of palm kernel meal without causing significant changes in sensorial characteristics. However, the fatty acid profile of the meat was altered.

Cenostigma macrophyllum Tul. var. acuminata Teles Freire Fraction Leaves Stimulate Gastric Healing in Rats and Human Cell Cultures

Ana Flávia Seraine Custódio Viana,Hélio B Fernandes,Mariana H Chave,Daniel A Viana,Verlane G Santos,Ariadne C A Silva,Miriam T.P. Lopes,Rita de Cássia M Oliveira 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3

Cenostigma macrophyllum Tul. var. acuminata Teles Freire (Leguminosae–Caesalpinioideae) is a medicinal plant traditionally used for treatment of gastric ulcer. This study evaluated the ulcer-healing activity of the hydroalcoholic fraction of C. macrophyllum Tul. var. acuminata Teles Freire leaves (Cm-FHA) and the tea of the leaves of C. macrophyllum (Cm-tea), as well as the possible action of Cm-FHA, through in vitro models. Leaves of C. macrophyllum were dried and powdered to obtain the Cm-FHA. Subsequently, the Cm-FHA was characterized phytochemically and biologically. Besides, Cm-tea was prepared. The gastric healing effects of Cm-tea and Cm-FHA were analyzed using the model of acetic acid-induced gastric ulcer in rats. The human gastric adenocarcinoma (AGS) cell line was employed as an in vitro model. Cm-tea promoted a protective effect against gastric ulcers induced by absolute ethanol. Cm-FHA or Cm-tea (100 mg/kg/7 days) exhibited a significant healing effect on ulcers induced by acetic acid. In the histological analysis, gastric mucosa treated with Cm-FHA or Cm-tea advanced restoration of the mucosal epithelium. In vitro, lower concentrations of Cm-FHA stimulated cell proliferation in the BrdU assay and cell migration. Cm-tea and Cm-FHA present a significant gastric healing effect in in vivo and in vitro models.

Influence of category−heifers, primiparous and multiparous lactating cows−in a large-scale resynchronization fixed-time artificial insemination program

Márcio de Oliveira Marques,Fábio Morotti,Camila Bizarro da Silva,Mario Ribeiro Júnior,Rubens César Pinto da Silva,Pietro Sampaio Baruselli,Marcelo Marcondes Seneda 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

This study was conducted to evaluate the influence of category (heifers, primiparous or multiparous cows) on pregnancy rates in a large scaleresynchronization ovulation program. Nelore heifers (n = 903), primiparous lactating cows (n = 338) and multiparous lactating cows (n =1,223) were synchronized using a conventional protocol of estradiol/P4-based fixed-time artificial insemination (FTAI). Thirty days afterultrasonography, females who failed the first FTAI were resynchronized with the same hormonal protocol prior to a second FTAI. Thepregnancy status of each cohort was evaluated by ultrasonography 30 days after each FTAI. The average conception rate after the first FTAIand resynchronization was 80.5%. Heifers had a higher conception rate (85%) than primiparous (76%) or multiparous cows (78%; p = 0.0001). The conception rate after the first FTAI was similar among heifers (57%), primiparous cows (51%) and multiparous cows (56%; p = 0.193). After the second FTAI, heifers exhibited a higher conception rate (66%) than primiparous or multiparous cows (51%; p = 0.0001). These resultsdemonstrate the feasibility of resynchronization in large beef herds for providing consistent pregnancy rates in a short period of time. We alsodemonstrated that ovulation resynchronization 30 days after FTAI is particularly effective for heifers, providing a conception rate of up to66%.

Complete androgen insensitivity syndrome and risk of gonadal malignancy: systematic review

Barros Beatriz Amstalden,Oliveira Letícia Ribeiro de,Surur Cíntia Regina Crocetti,Barros-Filho Antonio de Azevedo,Maciel-Guerra Andrea Trevas,Guerra-Junior Gil 대한소아내분비학회 2021 Annals of Pediatirc Endocrinology & Metabolism Vol.26 No.1

Complete androgen insensitivity syndrome (CAIS) is a rare condition characterized by 46,XY karyotype, female external genitalia, absence of uterus, and testes located intra-abdominally, in the inguinal ring or in the labia majora. In the present study, the frequency of testicular malignancy in prepubertal and pubertal patients with CAIS who underwent gonadectomy or gonadal biopsy were evaluated. Systematic review was performed using electronic databases according to the PRISMA-P (Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols) guidelines. The samples included 15 articles published between 1998 and 2019. From a total of 456 patients who underwent gonadectomy or gonadal biopsy, 6.14% had a premalignant lesion and most were postpubertal (82.14%). A malignant lesion was found in 1.3% and all were postpubertal. Because the risk of malignancy is very low in prepubertal patients with CAIS, gonadectomy may be delayed until puberty is complete, allowing it to progress naturally; however, close follow-up of the patient is required.

Identification and Characterization of a Class III Chitin Synthase Gene of Moniliophthora perniciosa, the Fungus That Causes Witches' Broom Disease of Cacao

Catiane S. Souza,Bruno M. Oliveira,Gustavo G. L. Costa,Albert Schriefer,Alessandra Selbach-Schnadelbach,Ana Paula T. Uetanabaro,Carlos P. Pirovani,Gonçalo A. G. Pereira,Alex G. Taranto,Júlio Cézar de 한국미생물학회 2009 The journal of microbiology Vol.47 No.4

Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transcription product, and its putative protein in basidioma and secondary mycelium stages of M. perniciosa, a basidiomycotan fungus that causes witches’ broom disease of cacao.

Structural complexity of the craniofacial trabecular bone in multiple myeloma assessed by fractal analysis

Michels Mariane,Morais-Faria Karina,Rivera César,Brandão Thaís Bianca,Santos-Silva Alan Roger,Oliveira Matheus L 대한영상치의학회 2022 Imaging Science in Dentistry Vol.52 No.1

Purpose: This study aimed to evaluate the structural complexity of craniofacial trabecular bone in multiple myeloma by fractal analysis of panoramic and lateral skull radiography, and to compare the fractal dimension values of healthy patients (HPs), pre-treatment patients (PTPs), and patients during bisphosphonate treatment (DTPs). Materials and Methods: Pairs of digital panoramic and lateral skull radiographs of 84 PTPs and 72 DTPs were selected. After application of exclusion criteria, 43 panoramic and 84 lateral skull radiographs of PTPs, 56 panoramic and 72 lateral skull radiographs of DTPs, and 99 panoramic radiographs of age- and sex-matched HPs were selected. The fractal dimension values from panoramic radiographs were compared among HPs, PTPs, and DTPs and between anatomical locations within patient groups using analysis of variance with the Tukey test. Fractal dimension values from lateral skull radiographs were compared between PTPs and DTPs using the Student t-test. Pearson correlation coefficients were used to assess the relationship between the mandible from panoramic radiographs and the skull from lateral skull radiographs. Intra-examiner agreement was assessed using intraclass correlation coefficients (α = 0.05). Results: The fractal dimension values were not significantly different among HPs, PTPs, and DTPs on panoramic radiographs or between PTPs and DTPs on lateral skull radiographs (P>0.05). The mandibular body presented the highest fractal dimension values (P≤0.05). The fractal dimension values of the mandible and skull in PTPs and DTPs were not correlated. Conclusion: Fractal analysis was not sensitive for distinguishing craniofacial trabecular bone complexity in multiple myeloma patients using panoramic and lateral skull radiography.