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Purge & Trap-GC/MS 에 의한 금호강 수계의 휘발성 유기물 분석
이상학,배준웅,전치완,한종환,최상섭,정혜영 경북대학교 환경과학연구소 1996 環境科學硏究所論文集 Vol.10 No.1
Improved sample introduction system has been investigated for the determination of 54 volatile organic compounds(VOC) in the Kumho river using purge & trap preconcentration apparatus and capillary gas chromatography/mass spectrometry. To solve the problems such as improper separation of peaks due to the adsorption of water and contamination of purge & trap system, the purge & trap apparatus was directly connected to the gas chromatograph. A carbopack B/car6oxen 1000 and 1001 trap were used for the purge & trap procedure and a custom made dimethyldiphenylpolysiloxane crosslinking moiety capillary column was used for the separation of compounds. The optimum analytical conditions were investigated and the method detection limits for the 54 VOC were obtained using these conditions. The present method was applied to the analysis of samples collected from 13 sites of the Kumho river. Among the 54 VOCs only dichloromethane, trichloromethane. trichloroethene. toluene, xylene and naphthalene were detected.
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Kang, Jeong-Han,Kuh, Sung-Uk,Shin, Zun-Zae,Cho, Yong-Eun,Yoon, Young-Sul,Chin, Dong-Kyu The Korean Neurosurgical Society 2005 Journal of Korean neurosurgical society Vol.38 No.4
Objective : The vertebroplasty is an excellent procedure in spine compression fracture, but there are some problems such as cement leakage and difficulties in bone biopsy. Recently, the osteoplasty system is developed to solve these problems, so we will report the usefulness of the osteoplasty system. Methods : From January 2003 to November 2003, there are 9patients with simple osteoporotic spine compression fracture, 2 compression fracture patients combined with suspicious spinal metastasis, 1patient with suspicious primary bone tumor, and 2patient with infection on spine. All patients were treated using the osteoplasty system. Results : All 11 compression fracture patients were relived the back pain after the osteoplasty and there is no complication. The bone biopsies in 3 suspicious cancer patients were also effectively performed using the osteoplasty system; negative result in 2patients and positive result in 1patient. The culture result of spontaneous discitis was no growth for 48hours. The spine tuberculosis was confirm using the osteoplasty system. Conclusion : The osteoplasty system has distinguished advantages in comparison with the vertebroplasty. That is, the risk of cement leakage is lower than vertebroplasty because of low pressure delivery system. And we can obtain the specimen effectively in bone biopsy because of large cannula. In conclusion, we emphasize that the osteoplasty system is a more useful procedure in spine compression fracture especially in the patient needed bone biopsy for diagnosis.
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자궁경부상피내종양 치료 후 재발 예측인자로서 인유두종 바이러스 검사
김지연 ( Ji Yeon Kim ),김수녕 ( Soo Nyung Kim ),이선주 ( Sun Ju Lee ),권한성 ( Han Sung Kwon ),전경훈 ( Kyung Hun Zun ),이지영 ( Ji Young Lee ),손인숙 ( In Sook Sohn ),이효표 ( Hyo Pyo Lee ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.10
목적: 인유두종 바이러스 (Human papillomavirus, HPV) 검사가 CIN 치료 후 재발 및 잔류병소의 예측을 위한 검사로 유용한지를 확인하는데 있다. 연구 방법: 대상 환자는 전체 561예 중 끝까지 추적되어 유용한 정보를 가진 452예가 되었고, 자궁경부 세포진 검사와 HPV DNA 검사 그리고 질확대경하 조직검사가 시행되었다. 여기서 HPV DNA 검사와 자궁경부 세포진 검사 (Papanicolaou smear test, Pap smear)의 민감도와 특이도 및 우도비를 비교하였다. 결과: 조직검사결과 14예가 재발 및 잔류병소로 나타났다. 조직학적으로 mild dysplasia-CIN 1이 7예에서, moderate dysplasia-CIN 2가 5예에서 그리고 severe dysplasia-CIN 3이 2예로 판명되었다. HPV DNA 검사의 민감도와 특이도는 각각 92.9% (95% 신뢰구간 68.5, 98.7), 75.3% (71.1%, 79.1%)이었고 세포진 검사의 민감도와 특이도는 71.4% (45.4%, 88.3%), 92.5% (89.6%, 94.6%)였다. HPV DNA 검사의 양성 우도비는 3.77 (3.03,4.69)이었고 음성 우도비는 0.09 (0.01,0.63)였다. 세포진 검사의 양성 우도비는 9.48 (5.95,15.11)이었고 음성 우도비는 0.31 (0.13.0.71)였다. HPV DNA 검사와 세포진 검사의 정확도는 각각 78.3%, 94.7%였다. HPV DNA와 세포진 검사를 동시에 본 병합 검사의 민감도, 특이도, 양성 우도비 및 음성 우도비는 각각 92.9% (68.5%, 98.7%), 73.1% (68.7%, 77.0%), 3.45 (2.79,4.26), 0.10 (0.01,0.65)이었다. 결론: HPV DNA 검사는 민감도가 높고 음성 우도비가 낮아 검사 결과가 음성인 경우 재발 및 잔류병소의 가능성이 매우 낮다고 여겨진다. HPV DNA 음성인 경우 특이도가 낮아 정확도를 높이기 위해서는 세포진 검사가 필요하다. 두 검사 모두에서 음성인 경우 정기적인 검사를 할 수 있다. Objective: The aim of this study was to evaluate high-risk (HR) HPV DNA test to predict recurrence/residual disease in patients treated for CIN (cervical intraepithelial neoplasia). Methods: Four hundred and fifty-two patients treated with LLETZ (large loop excision of the transformation zone) were followed by HR HPV DNA test, cytology and colposcopy. The sensitivity, specificity and diagnostic odds ratios in predicting recurrence/residual disease were compared to those of cytology and HPV DNA test. Results: Fourteen patients (3.1 %) developed recurrent/residual disease, during follow up. Of these women, 7 were diagnosed at the time of recurrence with a CIN 1 lesion, 5 with a CIN 2 lesion, and 2 with a CIN 3 lesion. The sensitivity and specificity of the HPV DNA test were 92.9% (CI 68.5%, 98.7%) and 75.3% (71.1%, 79.1%). The sensitivity and specificity of the cytology were 71.4% (45.4%, 88.3%) and 92.5% (89.6%, 94.6%), respectively. The likelihood ratio of a positive and negative HPV DNA test were 3.77 (3.03, 4.69) and 0.09 (0.01, 0.63). And the likelihood ratio of a positive and negative cytology were 9.48 (5.95, 15.11) and 0.31 (0.13, 0.71). The accuracy of cytology and HPV DNA test were 94.7% and 78.3%. The sensitivity and specificity of the combination test (PAP and/or HPV DNA test) were 92.9% (68.5%, 98.7%) and 73.1% (68.7%, 77.0%). The likelihood ratio of a positive and negative combination test were 3.45 (2.79, 4.26) and 0.10 (0.01, 0.65). Conclusion: Cytology remains the base in the follow up after of CIN. HPV DNA test increase the sensitivity of cytology. Negative HPV test can rule out recurrent/residual disease.
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