Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples

Hayriye Yesim Can,Mehmet Elmalı,Alper Karagoz 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.2

The objectives of this study were: i) to detect the presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk, cheese, beef minced meat, and chicken meat samples; ii) to evaluate the antimicrobial susceptibility of the isolates; and iii) to determine clonal relation among the isolates by using pulsed-field gel electrophoresis (PFGE) method. Therefore, a total of 160 food samples were randomly collected between August 2014 and May 2015 in Hatay province, located in the southern Turkey. Twenty (12.5%) of the samples were found to be contaminated with S. aureus. A total of 40 isolates from the 20 positive samples were confirmed to be S. aureus by multiplex PCR based on 16S rRNA and nuc gene. The mec A gene was not detected in any of the S. aureus strains. In the present study, 39 out of 40 (97.5%) isolates were found to be resistant to one or more antibiotics. All of isolates were susceptible to gentamicin, oxacillin, and vancomycin. The highest resistance rate was detected in penicillin (95%) and ampicillin (92.5%), followed by tetracycline (30%), erythromycin (20%), ciprofloxacin (12.5%). Nine major patterns were determined by PFGE. In 6 of these patterns, thirty-six strains (90%) had identical PFGE profiles.

Poster Session : PS 0396 ; Infectious Disease ; A Rarely Seen Empyema Cause: Leuconostoc Mesenteroides

( Hanife Usta Atmaca ),( Feray Akbas ),( Yesim Karagoz ),( Betul Borku Uysal ),( Mustafa Boz ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Case report: 64 year-old male patient was presented with cough, fever and sputum production. His medical history was unremarkable. He was producing and selling pickled vegetables. Only pathological fi nding in his physical examination was the reduction of pulmonary sounds in the lower part of left lung. Hemogram showed; leukocyte:5500/mm³ (neutrophil 65%, lymphocyte 30%), platelet: 287000/mm³, hematocrit 28%. Biochemical tests revealed normal, serum CRP level was 11,7 mg/dl. Pleural effusion was present in chest X-ray. Pleural ponction was performed. Gram-positive cocci and high concentration of leukocytes were seen in gram-staining in the exudative pleural fi uid. In the culture Leuconostoc mesenteroides was grown. CT showed pleural effusion and a consolidation containing air bronchogram. The patient was diagnosed with pleural empyema and drainage treatment with thorax tube and 2 grams of ceftriakson and 1 gr claritromycin daily were started. The symptoms regressed beginning from the fourth day. On the fourteenth day, CRP level returned to normal. The treatment was stopped at twenty-fi rst day with full recovery. Discussion: Leuconostocs are members of the family Streptococcacae and have only recently been recognized as potential pathogens. Infections by Leuconostoc species bacteria are uncommon, and usually affect patients with an underlying disease, or those fi tted with a venous catheter or subjects previously treated with vancomycin. Bacterias are found naturally in plants and vegetables and are used in milk, pickle and wine industries. While an underlying factor is needed for those organisms to become pathogenic, our case was completely healthy and had no invasive procedure previously. Because he was selling and directly producing pickles, it was considered that the organism could have passed directly. This case points out that; Leuconostoc mesenteroides should be considered as a causative agent in nasocomial infections, also in non- immunocompromised patients.

Poster Session:PS 0491 ; Nephrology : Congenital Agenesis of the Inferior Vena Cava in a Presenting with Acute Renal Failure

( Hanife Usta Atmaca ),( Feray Akbas ),( Yesim Karagoz ),( Ebru Pekguc ),( Mustafa Boz ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Introduction: Agenesis of IVC (inferior vena cava) is a rare congenital anomaly which is generally incidentally found in asymptomatic patients. In patients with congenital intra-abdominal venous malformation, extreme care must be taken during radiological or surgical interventions. Case Report: A 35-year-old male patient was presented with nausea and vomiting following abdominal CT- performed for chronic abdominal pain-, presenting a subsequent decrease in urine output, and elevation of blood urea and creatinine. The patient had suffered from disseminated varicosities since the age of 15. In physical examination, intense subcutaneous venous collaterals on both lower extremities and abdomen and hepatosplenomegaly were observed. Grade 2 hydronephrosis in the right kidney and grade 4 hydronephrosis with decreased parenchymal thickness in the left kidney were present on renal ultrasonography. CT scan revealed discontinuity of contrast in the left portal vein compatible with portal vein thrombosis. On the left, ureteral continuity was not found. MR venography revealed an abnormally narrow IVC. The diagnosis was established as contrast induced nephropathy and the patient underwent hemodialysis. After 3 sessions, serum urea and creatinine levels, renal functions, and urine excretion returned to normal. The patient was discharged and a follow-up was planned. Discussion: Recognition of vascular abnormalities is important to prevent related complications. Agenesis of IVC enhances the risk of acute renal failure; regarding decreased renal fi ltration due to insuffi ciency of venous return and the mass effect on ureters, Extreme caution or even total abstinence for contrast media injection is necessary. Surgeons and interventional radiologists should be warned against potential complications.

The Prevalence of Lumbar Paraspinal Muscle Fatty Degeneration in Patients with Modic Type I and I/II End Plate Changes

Ibrahim Burak Atci,Hakan Yılmaz,Mustafa Yavuz Samanci,Aysel Gurcan Atci,Yesim Karagoz 대한척추외과학회 2020 Asian Spine Journal Vol.14 No.2

Study Design: Retrospective case control. Purpose: The authors of this study assessed whether the prevalence of paraspinal fatty degeneration correlates with the presence of Modic type I and I/II change in patients with low back pain (LBP). Overview of Literature: Modic changes are bone marrow and end plate changes visible on magnetic resonance imaging. Methods: A consecutive series of 141 patients who attended the neurosurgery outpatient clinic between April 2017 and September 2017 for nonspecific LBP were evaluated. Sixty-one patients with single-level Modic type I or I/II change constituted the patient group. Eighty age-, gender-, and body mass index (BMI)-matched patients without any Modic changes were recruited as the control group. A retrospective review was performed in 61 patients with Modic changes and 80 controls without Modic changes. The percentage of fatty muscle degeneration was graded by two reviewers using T2-weighted axial images at the L4–L5 level. The system was graded as follows: grade 0, normal; grade I, minimal focal or linear fat deposition; grade II, up to 25%; grade III, 25%–50%; and grade IV, more than 50%. Results: Sixty-one patients with nonspecific LBP and Modic type I or I/II change and 80 patients without Modic changes were evaluated. There was no difference between these groups in terms of age, gender, and BMI distribution. The mean muscle cross-sectional area in the patient and control groups were 1,507.37±410.63 and 1,681.64±379.69. Regarding fatty degeneration, a chi-square test of homogeneity was run, and the two multinomial probability distributions were not equal within the population analyzed. Conclusions: The novel finding of this investigation is that patients with Modic type I and I/II changes have greater amounts of fatty degeneration in their lumbar paraspinal musculature.