복와위 상태 중환자에서의 영양공급에 따른 임상적 예후 분석

이연주 ( Yeonju Lee ),김형숙 ( Hyung-sook Kim ),남궁형욱 ( Hyungwook Namgung ),이은숙 ( Eun Sook Lee ),김은경 ( Euni Lee ),조영재 ( Young-jae Cho ),이연주 ( Yeon Joo Lee ) 한국정맥경장영양학회 2018 한국정맥경장영양학회지 Vol.10 No.1

Purpose: Enteral nutrition is recommended in critically ill patients. On the other hand, the recommendation of nutritional support is limited and often controversial in critically ill patients in the prone position. Therefore, this study evaluated the clinical outcomes of nutritional support in critically ill patients in the prone position. Methods: A retrospective evaluation of the electronic medical records was conducted, including adult patients who were in the medical intensive care unit (ICU) in the prone position in Seoul National University Bundang Hospital from May 1, 2015 to June 30, 2017. The patients’ characteristics, nutritional support status while they were in the prone position, mortality in ICU and during hospitalization, ICU length of stay, mechanical ventilation days, and complications, such as ventilator associated pneumonia (VAP) and vomiting were collected. Results: In total, 100 patients were included. Of these, 12 received enteral nutrition and parenteral nutrition and 88 received only parenteral nutrition. The groups were similar in terms of age, sex, number of comorbidity, weight, PaO₂/FiO₂, hours of prone position, Simplified Acute Physiology Score II (SAPS II), Acute Physiologic and Chronic Health Evaluation II (APACHE II) score, and Sequential Organ Failure Assessment (SOFA) score. No differences were observed in ICU mortality (75.0% vs. 46.6%; P=0.065), hospital mortality (83.3% vs. 58.0%; P=0.081), ICU length of stay (22.2±14.6 vs. 18.2±21.2; P=0.128) and mechanical ventilation days (19.3±14.8 vs. 14.5±19.1; P=0.098). In addition, there were no differences in the possible complications of the prone position, such as VAP (8.3% vs. 4.5%; P=0.480) and vomiting (8.3% vs. 1.1%; P=0.227). Conclusion: No significant differences in the clinical outcomes were observed. Further studies will be needed to confirm the way of nutrition support while in the prone position.

동화활용 언어프로그램이 유아의 창의성 향상에 미치는 효과

이경화(Lee Kyunghwa),이연주(Lee Yeonju) 한국영재교육학회 2008 영재와 영재교육 Vol.7 No.1

  본 연구는 동화를 이용한 언어활동 프로그램이 유아의 창의성 향상에 미치는 효과를 확인함으로써, 유아의 창의성 향상을 위한 효과적인 교수프로그램 개발을 위한 자료로 제공하고자 하는데 목적이 있다. 따라서 본 연구에서는 동화활용 언어프로그램이 유아의 언어와 도형 영역에서의 창의성 향상에 효과적일 것이라는 가설을 설정하고 이를 검정하였다. 본 연구의 대상은 유아 12명으로, 이들을 무선 선발하여 실험집단과 통제집단에 각 6명씩 무선 배치한 후 사전검사를 실시하여 동질성을 확인하였다. 수업은 주 2회씩 총 12회에 걸쳐 실험을 실시하였다. 연구 결과, 동화활용 언어프로그램이 유아의 언어와 도형영역에서의 창의적 능력과 창의적 성격 향상에 효과적임이 확인되었다. 따라서 유아의 창의성을 계발시키고자 하는 수업에 본 연구에서 개발된 것과 같은 동화활용 언어프로그램을 적용한다면 유아교육현장에서 효과적으로 사용할 수 있을 것이다.   The objective of this study is to certify what effects language activity programs have on progress in children"s creativity. That is, this study aims at finding an effective teaching method for progress in creativity by clarifying whether telling fairy tales to children and making them participate in language activities have effects on the development of a rich imagination, curiosity, and creative ideas, and on the formation of creative personality.<BR>  The hypothesis of this study was that language activity programs using fairy tales would affect progress in children"s linguistic and creative abilities in a figural field and their creative personality.<BR>  A total of twelve 5-year-old children were randomly sampled and the respective six subjects were then classified into an experimental group which applied language activity programs using fairy tales and a control group where teachers mainly led language activities. These experiments were conducted twice every week for 6 weeks from March 23rd to April 27th, 2007, amounting to 12 sessions.<BR>  As instruments, "Integrated Creativity Test for Children" developed by Lee Kyung-hwa and Lee Shin-dong(2003) was used for both pre-and post- test. Then data was treated by SPSS WIN 12.0 to conduct the t-test.<BR>  The results of this study were summarized as the following:<BR>  First, children in the experimental group who participated in language activity programs using fairy tales increased their creative ability(linguistic creativity and figural creativity) more than the control group where the teacher led general language activities.<BR>  Second, children in the experimental group who participated in language activity programs using fairy tales showed progress in their linguistic creativity (imagination, fluency, and originality) more than the control group. That is, the language activity programs using fairy tales had positive effects on progress in linguistic creativity.<BR>  Third, children in the experimental group who participated in language activity programs using fairy tales showed progress in their figural creativity(continuity, connection, accomplishment, addition of new elements, themes, and nonhabituation) more than the control group. In other words, language activity programs using fairy tales had positive effects on progress in children"s figural creativity.<BR>  Fourth, children in the experimental group who participated in language activity programs using fairy tales improved their creative personality(curiosity, independence, adventurous spirit, and attachment to assignments) more than the control group. That is, the language activities programs using fairy tales had positive effects on progress in children"s creative personality.<BR>  In conclusion, it was verified that the language activity programs using fairy  tales had positive effects on the progress of the children"s creativity. Therefore, the program is expected to be used as a teaching method for a progress in children"s creativity in the field of early childhood education.

PNF 상지패턴에 기초한 진동운동기구 훈련이 노인의 체간 안정성과 균형에 미치는 영향

이형수 ( Hyoungsoo Lee ),임정대 ( Jungdae Im ),이기훈 ( Gihun Lee ),이지성 ( Jiseong Lee ),이진욱 ( Jinwuk Lee ),손지윤 ( Yeonju Seo ),서연주 ( Jiyoon Son ),안효정 ( Hyojoeng An ),오현정 ( Hyunjeong Oh ),윤하늘 ( Haneul Youn ),이서연 대한통합의학회 2015 대한통합의학회지 Vol.3 No.3

Purpose : This study examines the effect of vibration exercise grafting PNF patterns for 6 weeks on upper body stability and equilibrium for seniors having fifteen or over of MMSE-K. Method : A total of 10 senior citizens participated in this study. Each participant performed PNF patterned exercises using vibration sports equipment for 30 minutes, once static a week, for six weeks. We measured trunk stability and balance degree before and after the six-week exercise program. Motor Assessment Scale (MAS) was used to measure trunk stability, while Functional Reach Test (FRT) and Timed Up and Go (TUG) was used to measure balance degree. The collected data was processed using paired t-test to confirm the difference between pre-program conditions and post-program conditions. Results: The results of our study show that post-program trunk stability measurements increased when compared to pre-program data; however, this increase was not statistically significant. pre and post-measurements for satatic balance and dynamic balance were statistically unchanged. Conclusion: Due to limitations in the number of participants, the procedural design of this experiment, and the limited amount of time participants actually controlled, this study failed to produce statistically significant results. However, further study should be conducted using a systematically implemented exercise program to show support for exercising with flexi-bar as an effective program for the elderly.

Marker Assistant Evaluation of Fusarium Head Blight Resistance in Korean Wheat Cultivars

Yeonju Jung,Chul Soo Park,Ji-Ung Jeung,Yong-Won Seo,Myung-Chul Lee,Jeong-Ran Lee,Gi-An Lee,Jung-Gon Kim 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Fusarium head blight (FHB) is the most important disease of wheat that may cause serious yield and quality losses and leads to harmful contamination of the grain with fungal toxins. Korean wheat cultivars showed much less resistant to FHB than Sumai3, which known as resistance to FHB, evaluated by spray inoculation in our companion report. Many DNA-marker studies, including sequence tagged site (STS) and simple sequence repeat (SSR) markers and qualitative trait loci (QTL) associated with FHB resistance have been identified and mapped on the specific chromosome, especially 3BS, by many previous studies. However, there was no application of DNA-markers to select FHB resistance lines in Korean wheat breeding programs. 3 SSR markers and 6 STS markers linked to major QTL on chromosome arms 3BS found in Sumai3 and its derivatives were used to screen FHB resistance in Korean wheat cultivars. All markers known to be most significant markers based on previous research (Liu and Anderson 2003). There was general lack of marker polymorphism for Korean wheat cultivars used in this study. However, STS3B-138 marker only showed the polymorphism in Korean wheat cultivars. Allele size of STS3B-138 is 355 bp in Sumai 3 and 12 of 24 cultivar showed this allele. The range in Type II resistance rating was 1-5 and average of these 12 cultivars have 355bp allele was grade 2.4. The most resistant cultivars under the 2 grade, Gobun, Jinpoom, Milseong and Namhae also showed 355bp allele. Therefore, These results provide potential for development of wheat cultivars with improved Fusarium head blight resistance through use of effective FHB screen methods and marker - assisted selection.

Cyclization of Peptide-Tagged Proteins for Improving Stability and Permeability in Cells and Liposomes

Yeonju LEE,Kyung-Min KIM,Hyun-Jung SEONG,Chaeyeon KIM,Young-Pil KIM 한국생물공학회 2023 한국생물공학회 학술대회 Vol.2023 No.4

Administration of Glucosylceramide Ameliorated the Memory Impairment in Aged Mice

Lee, Yeonju,Oliynyk, Sergiy,Jung, Jae-Chul,Han, Jeong Jun,Oh, Seikwan Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>The function and the role of glucosylceramide have not been well studied in the central nervous system. This study was aimed to investigate the possible roles of glucosylceramide in memory function in aged mice. Glucosylceramide (50 mg/kg, p.o.) showed memory enhancing activity after 3-month treatment in the aged mice (C56BL/6, 18–20 months old) through Y-maze, novel objective test, and Morris water maze test. Long-term treatment of glucosylceramide decreased the expression of iNOS and COX-2 in the brain of aged mice. The LPS-induced mRNA level of iNOS, COX-2, IL-1<I><I>β</I></I>, and TNF-<I><I>α</I></I> was reduced by the acute treatment with glucosylceramide in adult mice. These results suggest that glucosylceramide plays an important role in anti-inflammatory and memory enhancement, and it could be a potential new therapeutic agent for the treatment of neurodegenerative diseases such as Alzheimer's disease.</P>

Cellular interactions of doxorubicin-loaded DNA-modified halloysite nanotubes.

Lee, Yeonju,Jung, Goo-Eun,Cho, Sang Joon,Geckeler, Kurt E,Fuchs, Harald RSC Pub 2013 Nanoscale Vol.5 No.18

<P>Halloysite nanotube (HNT)-based supramolecular complexes are synthesized and evaluated with respect to their cytotoxicity and effects on cellular structures. As HNTs are water-insoluble, DNA is applied for wrapping the surface of HNTs to enhance their water-dispersibility. To investigate the potential of DNA-wrapped HNTs (HD) as a promising drug delivery carrier, doxorubicin (DOX) is introduced as a model anticancer agent and loaded onto HD. The DOX-loaded, DNA-wrapped HNTs (HDD) show sustained DOX release over two weeks without initial burst of DOX indicating delayed DOX release inside cells. In addition, effects of DNA-wrapped HNTs (HD) or HDD on the cytoskeleton organization of A549 cells are studied by visualizing the distribution of F-actin filaments using confocal laser scanning microscopy, and cellular morphological changes are observed by scanning electron microscopy and scanning ion conductance microscopy.</P>

Administration of red ginseng ameliorates memory decline in aged mice

Lee, Yeonju,Oh, Seikwan The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.3

Background: It has been known that ginseng can be applied as a potential nutraceutical for memory impairment; however, experiments with animals of old age are few. Methods: To determine the memory enhancing effect of red ginseng, C57BL/6 mice (21 mo old) were given experimental diet pellets containing 0.12% red ginseng extract (approximately 200 mg/kg/d) for 3 mo. Young and old mice (4 mo and 21 mo old, respectively) were used as the control group. The effect of red ginseng, which ameliorated memory impairment in aged mice, was quantified using Y-maze test, novel objective test, and Morris water maze. Red ginseng ameliorated age-related declines in learning and memory in older mice. In addition, red ginseng's effect on the induction of inducible nitric oxide synthase and proinflammatory cytokines was investigated in the hippocampus of aged mice. Results: Red ginseng treatment suppressed the production of age-processed inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-${\alpha}$, and interleukin-$1{\beta}$ expressions. Moreover, it was observed that red ginseng had an antioxidative effect on aged mice. The suppressed glutathione level in aged mice was restored with red ginseng treatment. The antioxidative-related enzymes Nrf2 and HO-1 were increased with red ginseng treatment. Conclusion: The results revealed that when red ginseng is administered over long periods, age-related decline of learning and memory is ameliorated through anti-inflammatory activity.

Anti-Inflammatory and Neuroprotective Effects of Constituents Isolated from <i>Rhodiola rosea</i>

Lee, Yeonju,Jung, Jae-Chul,Jang, Soyong,Kim, Jieun,Ali, Zulfiqar,Khan, Ikhlas A.,Oh, Seikwan Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>To determine the biological activity of <I>Rhodiola rosea</I>, the protein expression of iNOS and proinflammatory cytokines was measured after the activation of murine microglial BV2 cells by LPS under the exposure of constituents of <I>Rhodiola rosea</I>: crude extract, rosin, rosarin, and salidroside (each 1–50 <I><I>μ</I></I>g/mL). The LPS-induced expression of iNOS and cytokines in BV2 cells was suppressed by the constituents of <I>Rhodiola rosea</I> in a concentration-dependent manner. Also the expression of the proinflammatory factors iNOS, IL-1<I><I>β</I></I>, and TNF-<I><I>α</I></I> in the kidney and prefrontal cortex of brain in mice was suppressed by the oral administration of <I>Rhodiola rosea</I> crude extract (500 mg/kg). To determine the neuroprotective effect of constituents of <I>Rhodiola rosea</I>, neuronal cells were activated by L-glutamate, and neurotoxicity was analyzed. The L-glutamate-induced neurotoxicity was suppressed by the treatment with rosin but not by rosarin. The level of phosphorylated MAPK, pJNK, and pp38 was increased by L-glutamate treatment but decreased by the treatment with rosin and salidroside. These results indicate that <I>Rhodiola rosea</I> may have therapeutic potential for the treatment of inflammation and neurodegenerative disease.</P>

Cytotoxicity and cellular uptake of lysozyme‐stabilized gold nanoparticles

Lee, Yeonju,Geckeler, Kurt E. Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of biomedical materials research. Part A Vol.a100 No.4

<P><B>Abstract</B></P><P>The particle size and surface properties of gold nanoparticles are critical factors for the interactions between nanoparticles and cells. To produce noncytotoxic gold nanoparticles, a straightforward method for the synthesis of gold nanoparticles designed involving the reduction and stabilization by a protein such as a lysozyme in conjunction with microwave irradiation. The cooperative combination of a lysozyme with a high affinity for metal ions and the microwave irradiation allowed to form biocompatible gold nanoparticles in an aqueous system. In addition, the cell toxicity and the cellular uptake pathways of the gold nanoparticles synthesized against mouse embryonic fibroblast NIH‐3T3 cells were studied and found to be taken up by receptor‐mediated endocytosis. In addition, the lysozyme‐stabilized gold nanoparticles are accumulated in the cytoplasm as well as the nucleus without any significant cytotoxicity. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.</P>