배가사리, Microphysogobio longidorsalis(잉어과)의 개체군 생태

송호복,손영목 西原大學校 基礎科學硏究所 2004 基礎科學硏究論叢 Vol.18 No.-

한강수계의 섬강, 송천 및 홍천강 상류에서 M. longidorsalis의 서식지 환경과 개체군 상태를 연구한 결과는 다음과 같다. M. longidorsalis의 주된 서식지는 Aa 또는 Aa-Bb형 하천의 여울지역으로 주된 동서 어류는 Z. temmincki, Z. platypus, P. herzi, C. splendidus등이었다. 전장 70㎜∼110㎜이하군은 만 1년생, 70㎜∼110㎜이상군은 만 3년생으로 분석되었다. 본 종은 암수 모두 만 2년생군 중 전장 80㎜이상이 되어야 성적으로 성숙하여 생식 능력을 갖는 것으로 나타났다. 산란기는 4월부터 5월까지(수온:15∼17℃)로 조사되었으며, 산란 성기는 4월로 추측되었다. 평균 포란수는 7,334개였으며, 큰 개체일수록 포란수가 증가하는 경향(y=200.32_(TL)-11693, R²=0.6916)을 나타내었고, 난소 안의 성숙난의 크기는 1.03±0.072㎜였다. 성비는 1:0.87로 암컷이 조금 우세하였다. 주된 먹이 생물은 부착조류였으며, 전장에 따른 먹이 생물의 구성에는 큰 차이가 없었다. Population ecology of Microphysogobio longidorsalis was investigated in the upper part of the Han River in Korea from 1993 to 2003. The habitat was a current area, and stream form was Aa or Aa-Bb. The major cohabitating fishes were Zacco temrnincki, Zacco platypus, and Pungtungia herzi. The total lengths in this population indicated that the below 70 ㎜ group is one year old, the 70∼110㎜ group is two years old, and the group longer than 110 ㎜ is over three years old. Males and females became sexually mature at sizes longer than 80 mm total length. The prosperous spawning season was April to March with the water temperature at 15∼17℃. The mean number of eggs in the ovaries was 7,334. The mean mature egg size was 1.03±0.072 ㎜. The sex ratio all the year round of female to male was 1 : 0.87. Stomach contents were mainly phytoplankton.

남한강 상류에 서식하는 연준모치, Phoxinus phoxinus의 성숙 및 생식생태

송호복,손영목 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.4

Maturity and reproductive ecology of Phoxinus phoxinus were investigated in the upper part of the South Han river in Korea from January to December, 2001. The habitat was characterized by low water temperature and a bottom structure of boulders, cobbles and pedbbles. The major cohabitation fish were Rhynchocypris kumgangensis and R. steindachneri. The total lengths in this population indicated that the below 40 ㎜ group is immature, the 40~65 ㎜ group is one year old, the 65~85 ㎜ group is two years old and the longer group than 85 ㎜ is over three years old. There were no differences in total length distribution between females and males. Males became sexually mature at 50 ㎜ and females at 65 ㎜ total length. Prosperous spawning season was April with the water temperature at 10~12℃. The mean number of eggs in the ovaries was 1.280. The sex ratio all the year round of females to males was 1:0.85. The current velocity of the spawning ground was 26~58 ㎝/sec and water depth was 12~70 ㎝. Eggs were laid underneath the immaculate pebbles. During spawning, the sex ratio was 1:3.83(females : males).

배가사리, Microphysogobio longidorsalis (잉어과)의 개체군 생태

송호복,손영목 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

한강수계의 섬강, 송천 및 홍천강 상류에서 M. longidorsalis의 서식지 환경과 개체군 생태를 연구한 결과는 다음과 같다. M. longidorsalis의 주된 서식지는 Aa 또는 Aa-Bb형 하천의 여울지역으로 주된 동서 어류는 Z. temmincki, Z. pltypus. P. herzi, C. Splendidus 등이었다. 전장 70㎜ 이하군은 만 1년생, 70~110㎜군은 만 2년생, 110㎜ 이상군은 만 3년생으로 분석되었다. 본 종은 암수 모두 만 2년생군 중 전장 80㎜ 이상이 되어야 성적으로 성숙하여 생식 능력을 갖는 것으로 나타났다. 산란기는 4월부터 5월까지(수온 : 15~17℃)로 조사되었으며, 산란 성기는 4월로 추측되었다. 평균 포란수는 7,334개였으며, 큰 개체일수록 포란수가 증가하는 경향(y = 200.32_(TL) - 11693, R^(2) = 0.6916)을 나타내었고, 난소 안의 성숙난의 크기는 1.03±0.072㎜였다. 성비는 1:0.87로 암컷이 조금 우세하였다. 주된 먹이 생물은 부착조류였으며, 전장에 다른 먹이 생물의 구성에는 큰 차이가 없었다. Population ecology of Microphysogobio longidorsalis was investigated in the upper part of the Han River in Korea from 1993 to 2003. The habitat was a current area, and stream form was Aa or Aa-Bb. The major cohabitating fishes were Zacco ternrnincki, Zacco platypus, and Pungtungia herzi. The total lengths in this population indicated that the below 70 ㎜ group is one year old, the 70-110㎜ group is two years old, and the group longer than 110 ㎜ is over three years old. Males and females became sexually mature at sizes longer than 80 ㎜ total length. The prosperous spawning season was April to March with the water temperature at 15-17℃. The mean number of eggs in the ovaries was 7,334. The mean mature egg size was 1.03f0.072㎜. The sex ratio all the year round of female to male was 1 : 0.87. Stomach contents were mainly phytoplankton.

CHEMISTRY : Protective effect of arabinose and sugar beet pulp against high glucose-induced oxidative stress in LLC-PK1 cells

( Yeong Bok Song ),( Boh Kyung Kim ),( Mi Jin Choi ),( Yeong Ok Song ),( Eun Ju Cho ) 부산대학교 김치연구소 2012 김치의 과학과 기술 Vol.15 No.-

The protective effects of L-arabinose and sugar beet (SBP) on high glucose-induced oxidative stress were investigated using LLC-PK cells. Under the high glucose-induced cellular oxdative model, the treatment of 45 mM of glucose led to the decrease in cell viability and increase in lipod peroxidation_However. L-arabinose and SBP significantly inhibited the high glucose-induced cytotoxicity and lipid per-oxidation. In addition, the formation of nitric oxide(NO) was increased by the treatment of 45mM glu-cose. while the treatment of L-arabinose or SBP inhibited signigicantly the NO formation compared with high glucose-treated control. The superoxide anion production of groups treated whth L-arabinose or SBP was significantly lower than that of the control treated with high glucose. Futhermore. L-arabinose and SBP elevated the glucose uptake. resulting in lower glucose concentration compared with non-treated control. High glucose levels induced the overexpressions of bax. inducible NO synthase and cyclooxygen-ase-2. but L-arabinose or SBP treatrnent down-regulated the expressions of these genes. Arabinose and SBP also inhibited the expression of nuclear factor-kappa B induced by 45mM glucose in LLC-PK cells. In particular, arabinose exhibited stronger inhibitory activities on high glucose-induced oxdative stress than SBP did. These findings indicate that L-arabinose and SBP are promising antioxidative agents with protective activities against hyperglycemia.

가압볶음 무말랭이 열수 추출물의 항산화 효과

송영복(Yeong-Bok Song),최정선(Jeong-Sun Choi),이지은(Ji-Eun Lee),노정숙(Jeong-Sook Noh),김미정(Mi-Jeong Kim),조은주(Eun-Ju Cho),송영옥(Yeong-Ok Song) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.8

무말랭이 및 가압볶음 무말랭이 열수 추출물의 항산화 효과를 in vitro와 LLC-PK₁ cellular system에서 살펴보았다. 무말랭이 열수 추출물과 가압볶음 무말랭이 열수 추출물의 라디칼 소거능을 IC??로 비교해 보았을 때 DPPH(646.70 vs 135.45 ㎍/mL), superoxide anion(896.10 vs 566.98 ㎍/mL) 및 hydroxyl radical(722.26 vs 531.84 ㎍/mL)에 대한 가압볶음 열수 추출물의 효과가 유의적으로 높은 것으로 나타났다(p<0.001). 이러한 유리기 소거효과는 LLC-PK₁ cell에서 pyrogallol, SNP 및 SIN-1 처리로 superoxide, nitric oxide 및 peroxynitrite를 생성하여 산화스트레스를 유발한 다음 무말랭이 및 가압볶음 무말랭이 열수추출물을 첨가하였을 때 농도 의존적으로 세포생존율이 증가하고, 과산화물 생성량이 감소하여 세포손상을 보호하는 효과가 관찰되었다. 가압볶음 무말랭이 열수 추출물의 산화손상에 대한 보호 효과는 무말랭이 열수추출물에 비해 모든 유리기에서 유의적으로 높았다(p<0.05). 이러한 무말랭이 열수추출물의 항산화효과는 무에 함유되어 있는 함황 물질, 유리아미노산, 배당체 등에 의한 것으로 생각되며 가압볶음 무말랭이 열수 추출물의 효과가 더 높은 이유는 무말랭이에 함유된 유효성분이 가압볶음에 의해 증가되고 더불어 볶음과정 중에서 생성된 maillard 생성물의 항산화성 때문으로 생각된다. 무말랭이의 환원당 및 유리아미노산 함량은 볶음 후 유의적으로 감소하였으며(p<0.05), 이에 반해 maillard 생성물의 중간산물인 5-hydroxymethyl-2-furfural(5-HMF) 함량은 무말랭이 열수 추출물에서는 검출되지 않았던 것이 가압볶음 무말랭이 열수 추출물에서는 0.57 ㎎/g이 측정되었다. 본 연구 결과가압볶음 무말랭이 차의 섭취는 체내 유리기에 의한 산화적 손상을 보호하는 효과가 높은 것으로 사료된다. The antiradical property of hot water extract from dried radish (DR) or dried radish roasted with pressure (DRRP) was investigated in vitro and in LLC-PK₁ cell system. The contents of total free amino acid and reducing sugar in DR were decreased by 72.86% and 3.17%, respectively, after pressurized roasting. In vitro test, IC?? for DR and DRRP for DPPH radical scavenging activity were 646.70 and 135.45 ㎍/mL, 896.10 and 566.98 ㎍/mL for superoxide anion radical, and 722.26 and 531.84 ㎍/mL for hydroxy radical, respectively. The radical scavenging effects of DRRP was significantly greater than those for DR (p<0.001). These radical scavenging effects of DR and DRRP were confirmed in LLC-PK₁ at which oxidative stresses were induced by superoxide, nitric oxide and peroxynitrite generated in the treatment of pyrogallol, SNP, and SIN-1, respectively. Cell viability was increased in the presence of DR or DRRP, dose dependently (p<0.05), and TBARS formation was decreased. The protective effects of DRRP against oxidative damage in LLC-PK₁ were greater than those of DR at the same concentration tested (p<0.05). This superior antiradical activity of DRRP might be due to the products produced during the pressurized roasting in addition to the antioxidative compounds originally present in the radish. 5-hydroxyl methyl furfural (5-HMF) known as an intermediate product of the maillard reaction was detected in DRRP (0.57 ㎎/g), but not from DR. In conclusion, daily consumption of DRRP may prevent oxidative damage by retarding oxidative stress.

가압볶음 무말랭이 열수 추출물의 항산화 효과

송영복 ( Yeong Bok Song ),최정선 ( Jeong Sun Choi ),이지은 ( Ji Eun Lee ),노정숙 ( Jeong Sook Noh ),김미정 ( Mi Jeong Kim ),조은주 ( Eun Ju Cho ),송영옥 ( Yeong Ok Song ) 부산대학교 김치연구소 2010 김치의 과학과 기술 Vol.13 No.-

The antiradical property of hot water extract from dried radish (DR) or dried radish roasted with pressure (DRRP) was investigated in vitro and in LLC-PK1 cell system. The contents of total free amino acid and reducing sugar in DR were decreased by 72.86% and 3.17%, respectively, after pressurized roasting. In vitro test, IC50 for DR and DRRP for DPPH radical scavenging activity were 646.70 and 135.45 μg/mL, 896.10 and 566.98 μg/mL for superoxide anion radical, and 722.26 and 531.84 μg/mL for hydroxy radical, respectively. The radical scavenging effects of DRRP was significantly greater than those for DR (p<0.001). These radical scavenging effects of DR and DRRP were confirmed in LLC-PK1 at which oxidative stresses were induced by superoxide, nitric oxide and peroxynitrite generated in the treatment of pyrogallol, SNP, and SIN-1, respectively. Cell viability was increased in the presence of DR or DRRP, dose dependently (p<0.05), and TBARS formation was decreased. The protective effects of DRRP against oxidative damage in LLC-PK1 were greater than those of DR at the same concentration tested (p<0.05). This superior antiradical activity of DRRP might be due to the products produced during the pressurized roasting in addition to the anti oxidative compounds originally present in the radish. 5-hydroxyl methyl furfural (5-HMF) known as an intermediate product of the maillard reaction was detected in DRRP (0.57 mg/g), but not from DR. In conclusion, daily consumption of DRRP may prevent oxidative damage by retarding oxidative stress.

Tardive Dyskinesia를 동반한 교합불안정 환자의 치험증례

송영복(Yeong-Bok Song) 대한턱관절교합학회 1995 구강회복응용과학지 Vol.11 No.1

Developmental Characteristics of Eggs and Yolk Sac Larvae of Korean Striped Bitterling, Acheilognathus yamatsutae (Cyprinidae), Spawning in Mussels

Song, Ho-Bok,Son, Yeong-Mok The Ecological Society of Korea 2005 Journal of Ecology and Environment Vol.28 No.4

This study investigated the characteristics of the eggs and yolk sac larvae of Korean striped bitterling, Acheilognathus yamatsutae, spawned and grown In mussels. The number of eggs in the ovary was small ($358{\pm}108$ SD). The eggs were oval and large, and the formation of the perivitelline space was narrow. The eggs were hatched at only 41 hours after fertilization but the hatched larvae were underdeveloped. The development of yolk projection and minute tubercles on the skin surface was notable, along with the vividly moving tail in the hatched larvae. The yolk projection and minute tubercles were disappeared upon enhancement of the motor ability of the larvae was enhanced. The formation of eyes and body pigments of the larvae was relatively delayed in comparison with that of other cyprinid larvae. After completely consuming the yolks the larvae escaped from the mussel for free swimming and exogenous feeding.

거제도의 담수어류상과 분포상의 특징

손영목,송호복 한국어류학회 1998 韓國魚類學會誌 Vol.10 No.1

거제도 지역의 하천을 대상으로 담수어류상과 분포상의 특징을 조사한 결과 총 10과 24종 2,366개체가 채집되었으며, 담수어가 7과 17종, 주연성 어류가 3과 7종이 었다. 우점종은 Zacco temmincki로서 428개체(상대 풍부도 : 18.1%)가 채집되었으며 아우점종은 Rhinogobius brunneus로 388개체(16.4%)였다. 그 밖의 우세종으로는 Iksookimia longicorpus(290개체, 12.3%), Oryzias latipes(178개체, 7.5%), Chaenogobius urotuenia(177개체, 7.5%), Carassius auratus(163개체, 6.9%) 등이었으며, 희소종은 Coreoleuciscus splendidus, Silurus microdorsalis, Coreoperca kawamebari 등이었다. 한국 고유종은 C. splendidus, I. longicorpus, S. microdorsalis, Liobagrus mediadiposalis, Liobagrus mediadiposalis ssp.1, Odontobutis platycephala 등 6종으로 전체 어종수의 25.0%를 차지하였다. 군집구조 분석 결과 종다양도는 연초천이 가장 높은 2.21이었으며, 우점도는 외포천과 아주천이 1.00으로 높게 나타났고, 균등도는 외포천이 0.87로 가장 높았다. Aa형의 하천이 대부분인 관계로 상, 중, 하류의 구분없이 중 상류성 어종이 우세하였으며, 서식 어종수는 하천의 유로가 길고 유량이 많을수록 증가하였다. 또한 거제도의 북동부에는 L. mediadiposalis, 서남부에는 L. mediadiposalis ssp.1이 서식하는 분리 분포상을 나타내었으며 이와같은 Liobagrus 속 어류의 분포양상의 중요성을 고지리와 관련하여 논의하였다. The freshwater fish fauna and distribution were investigated in Kojedo from 1982 to 1997. The collected fishes were classified into twenty four species of ten families. Dominant species was Zazcco temmincki (relative abundance : 18.1%) and subdominant species was Rhinogobius brunneus (16.4%). Also Iksookimia longicorpus (12.3%), Oryzias latipes (7.5%), Chaenogobius urotaenia (7.5%) and Carassius auratus (6.9%) were numerous. The endemic species of Korea were six species (25.0%) and endangered species were C. splendidus, S. microdorsalis and C. kawamebari. In this study, species diversity index was high at Yoncho stream, dominance index at Aju and Oaepo stream and evenness index at Oaepo stream in community structure. There was a tendency of fish distribution in Kojedo that more longer the stream and more larger the water volume were, there were more species and more individual numbers. It was considered that the dominance of upper and middle reach dwelling fishes resulted in the dominance of Aa type stream mainly Liobagrus mediadiposalis and L. mediadiposalis ssp. 1 showed different distribution pattern in Kojedo : that is, the former inhabited in east-northern part but the latter in south-western part separately. And the importance of this distribution pattern was discussed in relation to paleogeography.

Fe-B-Si 비정질 권철심의 경년 열화 연구

閔馥基(Bok-Ki Min),宋在成(Jae-Sung Song),鄭英鎬(Yeong-Ho Jeong),姜遠求(Won-Koo Kang) 대한전기학회 1994 전기학회논문지 Vol.43 No.3