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Yanli Liang,Ming Zhao,Jianli Wang,Mengmeng Sun,Shanshan Li,Yufen Huang,Lin Zhong,Maochu Gong,Yaoqiang Chen 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-
A series of the modified Pt/SiO2–Al2O3 diesel oxidation catalysts with different WO3 loading were prepared through co-impregnation method. With an optimum additive loading of 2 wt% WO3, the catalyst significantly improved the performance and its stability. Compared to the free-tungsten sample, the catalyst increased the maximum conversion of NO by 5–10% and decreased the reaction temperature for 30% NO conversion by 7–28 C. It was found that there exist the electron and structure interaction between platinum and tungsten species. The interactions can generate and maintain more Pt sites in metallic state for the catalytic oxidation with enhanced activity and stability.
( Yan Xin ),( Zhibin Sun ),( Qiongzhen Chen ),( Jue Wang ),( Yicheng Wang ),( Linfeng Luogong ),( Shuhuan Li ),( Weiliang Dong ),( Zhongli Cui ),( Yan Huang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11
A novel alkaline protease from Streptomyces sp. M30, SapHM, was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography, and DEAE-Sepharose chromatography, with a yield of 15.5% and a specific activity of 29,070 U/mg. Tryptic fragments of the purified SapHM were obtained by electrospray ionization quadrupole timeof- flight mass spectrometry. Nucleotide sequence analysis revealed that the gene sapHM contained 1,179 bp, corresponding to 392 amino acids with conserved Asp156, His187, and Ser339 residues of alkaline protease. The first 24 amino acid residues were predicted to be a signal peptide, and the molecular mass of the mature peptide was 37.1 kDa based on amino acid sequences and mass spectrometry. Pure SapHM was optimally active at 80°C in 50 mM glycine-NaOH buffer (pH 9.0), and was broadly stable at 0-50°C and pH 4.0-9.0. The protease relative activity was increased in the presence of Ni2+, Mn2+, and Cu2+ to 112%, 113%, and 147% of control, respectively. Pure SapHM was also activated by dimethylformamide, dimethyl sulfoxide, Tween 80, and urea. The activity of the purified enzyme was completely inhibited by phenylmethylsulfonyl fluoride, indicating that it is a serine-type protease. The Km and Vmax values were estimated to be 35.7 mg/ml, and 5 × 104 U/mg for casein. Substrate specificity analysis showed that SapH was active on casein, bovine serum albumin, and bovine serum fibrin.
Hai-Yan Chen,Yue-Feng Guan,Xue-Yong Huang,Yu-Ting Wu,Fen-Fei Wang,Ju-Fang Gao,Que Zhou,Zhong-Nan Yang,Jia-Yao Liu,Hong-Xia Zhang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3
We have characterized a new male-sterile mutant in Arabidopsis that exhibits conditional sterility but has restored fertility when drought-stressed. This mutant,multiple impairments in male reproduction 1 (mimr1),shows pleiotropic defects in both vegetative and reproductive development. Examination with dissecting and scanning electron microscopes revealed that its pollen grains are not effectively released from the anther locule after dehiscence, and anther differentiation is defective. Growth of the style and stamen filaments are also abnormal. Histological analysis demonstrated that these phenomena are due not only to a noticeably reduced extension of the stamen but also greater elongation of the pistil. Genetic analysis indicated that mimr1 is a single locus recessive nuclear mutant. The mutation can be mapped to a locus strongly linked to a 1200-kb region on Chromosome 3. Meta-analysis of expression patterning presented several candidate genes in that region. No mutants with similar phenotypes have previously been reported, suggesting that mimr1 is a novel male-sterile locus. Characterization of MIMR1 will provide further insights into the molecular basis for the development of plant reproductive organs.
Hai-Zhong Yu a,Yu-Ling Huang,Ning-Yan Li,Yan-Xin Xie,Cheng-Hua Zhou,Zhan-Jun Lu 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4
Cathepsins belong to a group of mammalian papain-like cysteine proteases that play an important role in the insect immune response. In the present study, we identified two cathepsin genes from the Diaphorina citri genome database, cathepsin-L (DcCath-L) and cathepsin-O (DcCath-O). DcCath-L encodes a DcCath-L protein consisting of 348 amino acid residues, and DcCath-O encodes a DcCath-O protein consisting of 329 amino acid residues. DcCaths contain two conserved domains, the Inhibitor_I29 and Pept_C1 domains. Phylogenetic tree analysis revealed that DcCath-L and DcCath-O were divided into two different groups: Cathepsin-L and Cathepsin-O. Reverse transcription quantitative polymerase chain reaction analysis showed that both DcCath-O and DCCath-L were highly expressed in the midgut, while lower expression was observed in other tissues. Developmental stage expression analysis suggested that DcCath-O was mainly expressed in third instar nymph and adult, and DcCath-L was highly expressed in first and fourth instar nymph. Following exposure to two different heat-killed bacteria (Escherichia coli and Staphylococcus aureus) and Candidatus Liberibacter asiaticus, the expression of DcCath-O and DcCath-L was significantly increased and showed differential expression patterns at different time points. In addition, silencing of DcCath-L obvious affected the gene expression of members of the Toll pathway, while knock down of DcCath-L has no significantly influence. Overall, these data provide valuable information for further functional studies of D. citri cathepsins.
Probabilistic fatigue life prediction of bearings via the generalized polynomial chaos expansion
Aodi Yu,Yan-Feng Li,Hong-Zhong Huang,Hui Tong,Qing Diao 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10
In this work, an uncertainty propagation method for the probabilistic fatigue life prediction is proposed on the basis of the generalized polynomial chaos theory, which takes into account the uncertainty of material parameters and can effectively estimate the probability density function and cumulative distribution function of the fatigue life of rolling bearings. First, posterior distributions of the material parameters are fitted by Bayesian theory and based on grey bootstrap sampling fatigue test data. Then, the collocation approximation method is used to solve the expansion coefficients. Furthermore, the polynomial chaos expansion under each stress level is substituted into the bearing fatigue life prediction model for the probabilistic fatigue life prediction. Finally, the proposed method is validited by rolling bearing fatigue tests under two loading conditions. Results demonstrated that the probabilistic fatigue lives of rolling bearings are well predicted by the proposed method under constant and variable loading conditions.
Hai Ming Huang,Yan Liu,Denghao Wu,Yue Zhong Wu,Tian Xin Wu 한국유체기계학회 2023 International journal of fluid machinery and syste Vol.16 No.1
Cavitation in the pump induces vibration and noise, which leads to the degradation of pump performance and damage to the impeller. Therefore, it is of great significance to accurately identify the cavitation state of the centrifugal pump. To determine the cavitation state using the vibration characteristic frequency of the centrifugal pump, it is necessary to accurately identify the characteristic frequency due to the noise's effect. Therefore, a cavitation fault diagnosis method of centrifugal pump based on a cohesive hierarchy algorithm was proposed. Firstly, Singular Value Decomposition (SVD) is used to de-noise the vibration signal. Then the root means square of the vibration signal after de-noising is obtained as the eigenvalue of the vibration signal. Secondly, the hierarchical clustering algorithm is used to classify the vibration eigenvalues and accurately identify the non-cavitation and cavitation states of centrifugal pumps. Finally, through experimental tests, the method can effectively and quickly identify the cavitation state of the centrifugal pump, with an accuracy of 95%. The study provides a new way for rapidly diagnosing centrifugal pump cavitation
Du, Yong,Yan, Shu-Mei,Gu, Wan-Yi,He, Fan,Huang, Li-Yun,Li, Mei,Yuan, Yan,Chen, Ren-Hui,Zhong, Qian,Li, Man-Zhi,Li, Yong,Zeng, Mu-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12
FADS1 (fatty acid desaturase 1) plays a crucial role in fatty acid metabolism, and it was recently reported to be involved in tumorigenesis. However, the role of FADS1 expression in esophageal squamous cell carcinoma (ESCC) remains unknown. In the current study, we investigated the expression and clinical pathologic and prognostic significance of FADS1 in ESCC. Immunohistochemical analyses revealed that 58.2% (146/251) of the ESCC tissues had low levels of FADS1 expression, whereas 41.8% (105/251) exhibited high levels of FADS1 expression. In positive cases, FADS1 expression was detected in the cytoplasm of cells. Correlation analyses demonstrated that FADS1 expression was significantly correlated with tumor location (p=0.025) but not with age, gender, histological grade, tumor status, nodal status or TNM staging. Furthermore, patients with tumors expressing high levels of FADS1had a longer disease-free survival time (p<0.001) and overall survival time (p <0.001). Univariate and multivariate analyses revealed that, along with nodal status, FADS1 expression was an independent and significant predictive factor (p<0.001). In conclusion, our study suggested that FADS1 might be a valuable biomarker and potential therapeutic target for ESCC.
Zhang, Xue-Zhong,Huang, Xin-En,Xu, Yan-Li,Zhang, Xiu-Qun,Su, Ai-ling,Shen, Zheng-Shan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objective: To investigate the efficacy and safety of voriconazole in treating Chinese patients with hematological malignancies and invasive aspergillosis. Methods: From March 2007 to April 2012, patients with diagnoses confirmed by CT, GM test and/or PCR assays, were recruited into this study. Aspergillosis of all patients were treated with voriconazole 6 mg/kg intravenous infusion (iv) every 12 h for 1 day, followed by 4 mg/kg IV every 12 h for 10-15 days; Then, switch to oral administration that was 200mg every 12h for 4-12 weeks. Efficacy and safety were evaluated according to Practice Guideline of Infectious Diseases Society of America. Results: The overall response rate of 38 patients after voriconazole treatment was 81.6%. The median time to pyretolysis was 4.5 days. Treatment related side effects were mild and found in only 15.8% of cases. No treatment related deaths occurred. Conclusions: Voriconazole can considered to be a safe and effective front-line therapy to treat patients with hematological malignancies and invasive aspergillosis. Alternatively it could be used as a remedial treatment when other antifungal therapies are ineffective.