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간암세포 약제유도성 아포토시스에서 Ubiquitin fusion gene의 역할
한규지 ( Xue Ji Han ),( Kyung Ran You ),( Jing Wen ),( Ming Ji Liu ),( Deuk Soo Ahn ),( Dae Ghon Kim ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-
<목적> 항암화확약제유도성 아포토시스 유발 관련 유전자의탐색과 그유전자의 기능을 파악하여 항암화확제의 항암효과를 증대시키는 치료전략에 이용할 수 Ubiquitin-ribosomal protein fusion gene (Uba) 은 간암세포주에서 HPR 유도성 아포토시스 유도시 그발현이 증가되는 바 아포토시스유도에서 Uba gene의 기능을 분석하여 아포토시스 유발을 조절할 수 있는 항암화학치료방법에 응용하는 데 그목적이 있다. <방법> Hep 3B ce
Paint Removal of Airplane & Water Jet Application
Xue, Sheng-Xiong,Chen, Zheng-Wen,Ren, Qi-Le,Su, Ji-Xin,Han, Cai-Hong,Pang, lei Korean Society for Fluid machinery 2014 International journal of fluid machinery and syste Vol.7 No.3
The paint removal and recoating are the very important process in airplane maintenance. The traditional technology is to use the chemical way corroding the paint with paint remover. For changing the defects, corrosion & pollution & manual working, of the traditional technology, the physical process which removes the paint of airplane with 250MPa/250kW ultra-high pressure rotary water jetting though the surface cleaner installed on the six axes robot is studied. The paint layer of airplane is very thin and close. The contradiction of water jetting paint removal is to remove the paint layer wholly and not damage the surface of airplane. In order to solve the contradiction, the best working condition must be reached through tests. The paint removal efficiency with ultra-high pressure and move speed of not damaged to the surface. The move speed of this test is about 2m/min, and the paint removal efficiency is about $30{\sim}40m^2/h$, and the paint removal active area is 85-90%. No-repeat and no-omit are the base requests of the robot program. The physical paint removal technology will be applied in airplane maintenance, and will face the safety detection of application permission.
Ji Ae Lim,Ho-Jang Kwon,Hyun-Joo Kim,Mina Ha,Xue Han 환경독성보건학회 2019 환경독성보건학회지 Vol.34 No.1
This study investigated the social outcomes of the Environmental Health Studies of National Industrial Complex (EHSNIC), which have been conducted by the National Institute of Environmental Research (NIER) in eight National Industrial Complex Areas (NICAs) since 2003. Eighteen sessions of focus-group interviews with 85 people were conducted from October 2016 to January 2017. Interviewees were stakeholders from eight NICAs and included resident representatives, environmental nongovernment organizations, local government officials, and environmental health and safety officers from companies. Interview results were divided into six categories: EHSNIC awareness, EHSNIC outcomes, EHSNIC limitations, EHSNIC continuation, EHSNIC improvement directions, and EHSNIC results use. They were then further indexed into 23 divisions. EHSNIC awareness varied across stakeholders. A major EHSNIC outcome is that a continued result database was established, which was used as a reference for environmental improvements. EHSNIC limitations included no proper healthcare actions taken during the EHSNIC study period, a lack of EHSNIC results disclosure, a failure to reflect local specificity, and a lack of validity in the results. Regarding EHSNIC continuation, all stakeholders said EHSNIC should be conducted continuously. EHSNIC improvement directions included conducting studies tailored to each NICA, identifying correlations between pollutant exposure and disease, increasing the sample size, and performing repeated studies. Regarding EHSNIC results use, respondents wanted to use the results as a reference to relocate residents, ensure distance between NICAs and residential areas, provide healthcare support, develop local government policies, and implement firms’ environmental controls. Since EHSNIC aims to identify the health effects of NICAs on residents and take appropriate actions, it should be continued in the future. Even during the study period, it is important to take steps to preventively protect residents’ health. EHSNIC also needs to reflect each NICA’s characteristics and conduct reliable research based on stakeholder participation and communication.
( Xue Feng Han ),( Bo Kun Lin ),( Gan Ji Ru ),( Zhi Biao Zhang ),( Yan Liu ),( Zhong Hu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.2
Enteromorpha polysaccharides (EP) extracted from green algae have displayed a wide variety of biological activities. However, their high molecular weight leads to a high viscosity and low solubility, and therefore, greatly restrains their application. To solve this problem, bacteria from the surface of Enteromorpha were screened, and an Alteromonas macleodii strain B7 was found to be able to decrease the molecular weight of EP in culture media. Proteins harvested from the supernatant of the A. macleodii B7 culture were subjected to native gel electrophoresis, and a band corresponding to the Enteromorpha polysaccharide lyase (EPL) was detected by activity staining. The enzyme identity was subsequently confirmed by MALDI-TOF/TOF mass spectrometry as the putative α-amylase reported in A. macleodii ATCC 27126. The amylase gene (amySTU) from A. macleodii B7 was cloned into Escherichia coli, resulting in highlevel expression of the recombinant enzyme with EP-degrading activity. AmySTU was found to be cold-adapted; however, its optimal enzyme activity was detected at 40oC. The α-amylase was highly stable over a broad pH range (5.5-10) with the optimal pH at 7.5-8.0. The highest enzyme activity was detected when NaCl concentration was 2%, which dropped by 50% when the NaCl concentration was increased to 16%, showing an excellent nature of halotolerance. Furthermore, the amylase activity was not significantly affected by tested surfactants or the presence of some organic solvents. Therefore, the A. macleodii strain B7 and its α-amylase can be useful in lowering EP molecular weight and in starch processing.
A novel ID-based multi-domain handover protocol for mesh points in WMNs
( Xue Zhang ),( Guangsong Li ),( Wenbao Han ),( Huifang Ji ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.7
Wireless mesh networks (WMNs) provide an efficient and flexible method to the field of wireless networking, but also bring many security issues. A mesh point may lose all of its available links during its movement. Thus, the mesh point needs to handover to a new mesh point in order to obtain access to the network again. For multi-domain WMNs, we proposed a new ID-based signcryption scheme and accordingly present a novel ID-based handover protocol for mesh points. The mutual authentication and key establishment of two mesh points which belong to different trust domains can be achieved by using a single one-round message exchange during the authentication phase. The authentication server is not involved in our handover authentication protocol so that mutual authentication can be completed directly by the mesh points. Meanwhile, the data transmitted between the two mesh points can be carried by the authentication messages. Moreover, there are no restrictions on the PKG system parameters in our proposed multi-domain ID-based signcryption scheme so our handover scheme can be easily applied to real WMNs circumstances. Security of the signcryption scheme is proved in the random oracle model. It shows that our protocol satisfies the basic security requirements and is resistant to existing attacks based on the security of the signcryption. The analysis of the performance demonstrates that the protocol is efficient and suitable for the multi-domain WMNs environment.