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Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10
<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>
Li-hua Xie,Xin Quan,Jie Zhang,Yan-yan Yang,Run-hong Sun,Ming-cong Xia,Bao-guo Xue,Chao Wu,Xiao-yun Han,Ya-nan Xue,Li-rong Yang 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.1
Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. TUBβ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of TUBβ expression. The expression profile of ExoPG assessed using TUBβ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.
Exact solution for free vibration of curved beams with variable curvature and torsion
Li-li Zhu,Ying-hua Zhao,Guang-xin Wang 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.3
For the purpose of investigating the free vibration response of the spatial curved beams, the governing equations are derived in matrix formats, considering the variable curvature and torsion. The theory includes all the effects of rotary inertia, shear and axial deformations. Frobenius’ scheme and the dynamic stiffness method are then applied to solve these equations. A computer program is coded in Mathematica according to the proposed method. As a special case, the dynamic stiffness and further the natural frequencies of a cylindrical helical spring under fixed-fixed boundary condition are carried out. Comparison of the present results with the FEM results using body elements in I-DEAS shows good accuracy in computation and validity of the model. Further, the present model is used for reciprocal spiral rods with different boundary conditions, and the comparison with FEM results shows that only a limited number of terms in the resultant provide a relatively accurate solution.
Li, Ai-Hua,Liu, Hong-Can,Xin, Yu-Hua,Kim, Song-Gun,Zhou, Yu-Guang International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.2
<P>A Gram-stain-positive, aerobic, non-spore-forming, short-rod-shaped bacterium, designated strain MP203<SUP>T</SUP>, was isolated from ice water of Midui Glacier in Tibet Autonomous Region, China. The strain was psychrotolerant, growing at 0–25 °C. 16S rRNA gene sequence analysis showed that strain MP203<SUP>T</SUP> was most similar to <I>Frigoribacterium faeni</I> NBRC 103066<SUP>T</SUP>, <I>Compostimonas suwonensis</I> KACC 13354<SUP>T</SUP>, <I>Frigoribacterium mesophilum</I> KCTC 19311<SUP>T</SUP>, <I>Marisediminicola antarctica</I> CCTCC AB 209077<SUP>T</SUP> and <I>Alpinimonas psychrophila</I> JCM 18951<SUP>T</SUP>, with similarities of 97.4, 97.2, 97.2, 97.1 and 97.1 %, respectively. The maximum-likelihood phylogenetic tree indicated that strain MP203<SUP>T</SUP> clustered with nine genera of the family <I>Microbacteriaceae</I>, namely <I>Frigoribacterium</I>, <I>Compostimonas</I>, <I>Marisediminicola</I>, <I>Alpinimonas</I>, <I>Frondihabitans</I>, <I>Clavibacter</I>, <I>Subtercola</I>, <I>Klugiella</I> and <I>Agreia</I><I>.</I> However, bootstrap analysis showed that there was no significance in the branching pattern of the linage comprising strain MP203<SUP>T</SUP> and any existing generic lineage of the family <I>Microbacteriaceae</I>. DNA–DNA hybridization results indicated levels of relatedness between strain MP203<SUP>T</SUP> and <I>Marisediminicola antarctica</I> CCTCC AB 209077<SUP>T</SUP>, <I>Frigoribacterium faeni</I> NBRC 103066<SUP>T</SUP>, <I>Frigoribacterium mesophilum</I> KCTC 19311<SUP>T</SUP>, <I>Compostimonas suwonensis</I> KACC 13354<SUP>T</SUP> and <I>Alpinimonas psychrophila</I> JCM 18951<SUP>T</SUP> were 25.8±7.3, 29.6±7.6, 19.7±6.7, 16.0±4.2 and 12.4±5.1 % (mean±<SMALL>sd</SMALL>), respectively. The G+C content of the genomic DNA was 64.1 mol%. Analysis of the cell-wall peptidoglycan revealed that the peptidoglycan structure of strain MP203<SUP>T</SUP> was B10 type with Gly[<SMALL>l</SMALL>-Hse]–<SMALL>d</SMALL>-Glu–<SMALL>d</SMALL>-DAB, containing 2, 4-diaminobutyric acid (DAB) as a diagnostic amino acid. The cell-wall sugars were rhamnose, ribose, mannose and glucose. The major fatty acids were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>16 : 0</SUB> and anteiso A-C<SUB>15 : 1</SUB>. An unusual compound identified as anteiso-C<SUB>15 : 0</SUB>-DMA (1, 1-dimethoxy-anteiso-pentadecane) was also present in strain MP203<SUP>T</SUP>. The predominant menaquinone was MK-10. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one unknown glycolipid and four unknown lipids were detected in the polar lipid extracts. As strain MP203<SUP>T</SUP> was distinguishable from phylogenetically related genera in the family <I>Microbacteriaceae</I> in terms of its physiological and chemotaxonomic characteristics and phylogenetic position, it was considered to represent a novel species of a new genus. Thus, the name <I>Glaciihabitans tibetensis</I> gen. nov., sp. nov. is proposed. The type strain of <I>Glaciihabitans tibetensis</I> is MP203<SUP>T</SUP> ( = CGMCC 1.12484<SUP>T</SUP> = KCTC 29148<SUP>T</SUP>).</P>
Li Li Han,En Tao Wang,Yang Li Lu,Yong Fa Zhang,Xin Hua Sui,Wen Feng Chen,Wen Xin Chen 한국미생물학회 2009 The journal of microbiology Vol.47 No.3
Adzuki bean (Vigna angularis) is an important legume crop native to China, but its rhizobia have not been well characterized. In the present study, a total of 60 rhizobial strains isolated from eight provinces of China were analyzed with amplified 16S rRNA gene RFLP, IGS-RFLP, and sequencing analyses of 16S rRNA, atpD, recA, and nodC genes. These strains were identified as genomic species within Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, and Ochrobactrum. The most abundant groups were Bradyrhizobium species and Sinorhizobium fredii. Diverse nodC genes were found in these strains, which were mainly co-evolved with the housekeeping genes, but a possible lateral transfer of nodC from Sinorhizobium to Rhizobium was found. Analyses of the genomic and symbiotic gene backgrounds showed that adzuki bean shared the same rhizobial gene pool with soybean (legume native to China) and the exotic Vigna species. All of these data demonstrated that nodule formation is the interaction of rhizobia, host plants, and environment characters.
Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2
Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.