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The fecal microbiota composition of boar Duroc, Yorkshire, Landrace and Hampshire pigs
Xiao, Yingping,Li, Kaifeng,Xiang, Yun,Zhou, Weidong,Gui, Guohong,Yang, Hua Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10
Objective: To investigate the effect of host genetics on gut microbial diversity, we performed a structural survey of the fecal microbiota of four purebred boar pig lines: Duroc, Landrace, Hampshire, and Yorkshire. Methods: The V3-V4 regions of the 16S rRNA genes were amplified and sequenced. Results: A total of 783 operational taxonomic units were shared by all breeds, whereas others were breed-specific. Firmicutes and Bacteroidetes dominated the majority of the fecal microbiota; Clostridia, Bacilli, and Bacteroidia were the major classes. Nine predominant genera were observed in all breeds and eight of them can produce short-chain fatty acids. Some bacteria can secrete cellulase to aid fiber digestion by the host. Butyric, isobutyric, valeric, and isovaleric acid levels were highest in Landrace pigs, whereas acetic and propionic acid were highest in the Hampshire breed. Heatmap was used to revealed breed-specific bacteria. Principal coordinate analysis of fecal bacteria revealed that the Landrace and Yorkshire breeds had high similarity and were clearly separated from the Duroc and Hampshire breeds. Conclusion: Overall, this study is the first time to compare the fecal microbiomes of four breeds of boar pig by high-throughput sequencing and to use Spearman's rank correlation to analyze competition and cooperation among the core bacteria.
Substrate effect on phonon in graphene layers
Xiao Guo,Yongsong Wang,Siwen You,Dingbang Yang,Guiping Jia,Fei Song,Weidong Dou,Han Huang 한국탄소학회 2023 Carbon Letters Vol.33 No.5
Graphene exhibits high carrier mobility and concentration as well as other remarkable properties. Among them, the thermal behaviors of phonon modes play important roles in the application of optical and electronic devices. Here, A–A stacked graphene were proved well by Raman investigation on G and 2D modes. Temperature-dependent Raman scattering measurements on graphene with various number of layers on different substrates were conducted in the temperature range of 80–460 K. The first-order temperature coefficient of single layer graphene (SLG) on SiO2/ Si substrate is obviously smaller than that on Cu foil, indicating that the substrate effect attributes a great impact on graphene phonon temperature dependence. The first-order temperature coefficients of multilayer graphene linearly decrease as the number of layers increases, attributed to the reduction of substrate effect in phonon behaviors, rather than to the anharmonic phonon–phonon (ph–ph) coupling or thermal expansion.
Topology-based Workflow Scheduling in Commercial Clouds
( Haoran Ji ),( Weidong Bao ),( Xiaomin Zhu ),( Wenhua Xiao ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.11
Cloud computing has become a new paradigm by enabling on-demand provisioning of applications, platforms or computing resources for clients. Workflow scheduling has always been treated as one of the most challenging problems in clouds. Commercial clouds have been widely used in scientific research, such as biology, astronomy and weather forecasting. Certainly, it is very important for a cloud service provider to pursue the profits for the commercial essence of clouds. This is also significantly important for the case of providing services to workflow tasks. In this paper, we address the issues of workflow scheduling in commercial clouds. This work takes the communication into account, which has always been ignored. And then, a topology-based workflow-scheduling algorithm named Resource Auction Algorithm (REAL) is proposed in the objective of getting more profits. The algorithm gives a good performance on searching for the optimum schedule for a sample workflow. Also, we find that there exists a certain resource amount, which gets the most profits to help us get more enthusiasm for further developing the research. Experimental results demonstrate that the analysis of the strategies for most profits is reasonable, and REAL gives a good performance on efficiently getting an optimized scheme with low computing complexity.
( Yi Xie ),( Weidong Xiao ),( Daquan Tang ),( Jiuyang Tang ),( Guoming Tang ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.5
Energy efficiency is one of the most critical issues in the design of wireless sensor networks. In object-tracking sensor networks, the data storage and query processing should be energy-conserving by decreasing the message complexity. In this paper, a Prediction-based Energy-conserving Approximate StoragE schema (P-EASE) is proposed, which can reduce the query error of EASE by changing its approximate area and adopting predicting model without increasing the cost. In addition, focusing on reducing the unnecessary querying messages, P-EASE enables an optimal query algorithm to taking into consideration to query the proper storage node, i.e., the nearer storage node of the centric storage node and local storage node. The theoretical analysis illuminates the correctness and efficiency of the P-EASE. Simulation experiments are conducted under semi-random walk and random waypoint mobility. Compared to EASE, P-EASE performs better at the query error, message complexity, total energy consumption and hotspot energy consumption. Results have shown that P-EASE is more energy-conserving and has higher location precision than EASE.
( Yi Xie ),( Guoming Tang ),( Daifei Wang ),( Weidong Xiao ),( Daquan Tang ),( Jiuyang Tang ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.6
Uncertainty is ubiquitous in target tracking wireless sensor networks due to environmental noise, randomness of target mobility and other factors. Sensing results are always unreliable. This paper considers unreliability as it occurs in wireless sensor networks and its impact on target-tracking accuracy. Firstly, we map intersection pairwise sensors` uncertain boundaries, which divides the monitor area into faces. Each face has a unique signature vector. For each target localization, a sampling vector is built after multiple grouping samplings determine whether the RSS (Received Signal Strength) for a pairwise nodes` is ordinal or flipped. A Fault-Tolerant Target-Tracking (FTTT) strategy is proposed, which transforms the tracking problem into a vector matching process that increases the tracking flexibility and accuracy while reducing the influence of in-the-filed factors. In addition, a heuristic matching algorithm is introduced to reduce the computational complexity. The fault tolerance of FTTT is also discussed. An extension of FTTT is then proposed by quantifying the pairwise uncertainty to further enhance robustness. Results show FTTT is more flexible, more robust and more accurate than parallel approaches.
Liu, Shanshan,Wu, Di,Li, Lin,Sun, Xiao,Xie, Weidong,Li, Xia 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.8
1-oxoeudesm-11(13)-eno-$12,8{\alpha}$-lactone (OEL), a novel eudesmane-type sesquiterpene compound, has been shown to inhibit the growth of some cancer cell lines and induce significant apoptosis. Here, we investigated the anticancer activities of OEL in human lung cancer cells. Our studies demonstrated that OEL induced both apoptosis and autophagy in A549 and H460 cells. OEL-induced autophagy was assessed by appearance of autophagic vacuoles, formation of acidic vesicular organelles, conversion of LC3-I to LC3-II, recruitment of LC3-II to the autophagosomes, and activation of autophagy genes. Furthermore, administration of autophagic inhibitor 3-methyladenine augments OEL-induced apoptotic cell death. The induction of autophagy and apoptosis by OEL links to NF-${\kappa}B$ activation and the generation of reactive oxygen species (ROS). Interruption of NF-${\kappa}B$ activation by specific inhibitor promotes apoptosis, but decreases autophagy. ROS antioxidants (N-acetylcysteine) attenuated both OEL-induced autophagy and apoptosis. Further experiments confirmed that OEL-induced activation of ROS was increased by NF-${\kappa}B$ inhibitor whereas NF-${\kappa}B$ activation was not affected by ROS inhibition. These findings suggest that OEL-elicited autophagic response plays a protective role that impedes cell death, and inhibition of autophagy could be an adjunctive strategy for enhancing the chemotherapeutic effect of OEL as an antitumor agent.
Xian Lin,Jinling Chen,Gengsheng Xiao,Yujuan Xu,Daobang Tang,Jijun Wu,Jing Wen,Weidong Chen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3
Ultrasonic-microwave synergistic extraction (UMSE) was optimized for the extraction of oligosaccharides from longan pulp (OLP). Box-Behnken design was used to evaluate the effects of temperature (35-55oC), ultrasonic time (5-25 min), and water to material ratio (10-30 mL/g) on the extraction efficiency of crude OLP. A regression model was developed and its validity was statistically demonstrated. Significant interaction between temperature and water to material ratio was observed. The following optimal conditions for the extraction yield of crude OLP were determined: extraction temperature 55oC, ultrasonic time 18.52 min, and water to material ratio 10 mL/g. The extracted OLP were purified for the determination of molecular weight distribution and antioxidant activity. Results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry revealed that the molecular weight distribution of the purified OLP ranged from m/z 495.138 to 795.511. The purified OLP exhibited a dose-dependent behavior in 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity.