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( Qiang Zhang ),( Yujuan Li ),( Fangxue Xu ),( Mengmeng Zheng ),( Xiaozhi Xi ),( Xuelan Zhang ),( Chunchao Han ) 한국균학회 2017 Mycobiology Vol.45 No.2
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and NH<sub>4</sub>NO<sub>3</sub> was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at 20.67 μg/L, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and NH<sub>4</sub>NO<sub>3</sub> was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of 20.63 ± 0.11 μg/L, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.
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Protein and polyphenols involved in sediment formation in cloudy litchi juice
Dan Zeng,Gengsheng Xiao,Yujuan Xu,Bo Zou,Jijun Wu,Yuanshan Yu 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4
Sedimentation is a major issue in juice production. This paper aims to study the mechanisms of precipitateformation during the storage of cloudy litchi juice. Thesediment concentration, relative turbidity, and f potentialwere analyzed. The supernatant and sediment were separatedto determine the contents of proteins and phenolics. The results showed that the amount of sediment increasedduring the storage. In addition, the total protein and totalphenolic content in the supernatant decreased, whereas theglutelin and total phenolic contents in the sedimentincreased significantly (p\0.05). Moreover, our resultsshowed that the amounts of procyanidin B2 and quercetin-3-O-rutinose-7-O-rhamnoside in the supernatant decreasednoticeably. However, these two substances could not bedetected in the sediment. In summary, the formation ofsediment from litchi juice is mainly caused by the slowdenaturation of proteins and the oxidation of procyanidinB2 and quercetin-3-O-rutinose-7-O-rhamnoside.
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Xian Lin,Jinling Chen,Gengsheng Xiao,Yujuan Xu,Daobang Tang,Jijun Wu,Jing Wen,Weidong Chen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3
Ultrasonic-microwave synergistic extraction (UMSE) was optimized for the extraction of oligosaccharides from longan pulp (OLP). Box-Behnken design was used to evaluate the effects of temperature (35-55oC), ultrasonic time (5-25 min), and water to material ratio (10-30 mL/g) on the extraction efficiency of crude OLP. A regression model was developed and its validity was statistically demonstrated. Significant interaction between temperature and water to material ratio was observed. The following optimal conditions for the extraction yield of crude OLP were determined: extraction temperature 55oC, ultrasonic time 18.52 min, and water to material ratio 10 mL/g. The extracted OLP were purified for the determination of molecular weight distribution and antioxidant activity. Results of matrix-assisted laser desorption ionization time-of-flight mass spectrometry revealed that the molecular weight distribution of the purified OLP ranged from m/z 495.138 to 795.511. The purified OLP exhibited a dose-dependent behavior in 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity.
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Liqin Tang,Haocheng Liu,Manqin Fu,Yujuan Xu,Jing Wen,Jijun Wu,Yuanshan Yu,Xian Lin,Lu Li,Zhibin Bu,Wanyuan Yang 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.11
The preparation process of yellow pigment (YP) from gardenia (Gardenia jasminoides) fruit was investigated, and the main components of YP were characterized by liquid chromatography-time of flight-mass spectrometer/mass spectrometer (LC-TOF–MS/MS). Furthermore, cytotoxic activity in HepG2 cells by induction of apoptosis was also evaluated. The preparation results indicated that the color value of YP was 498.34, which was 8.6 times higher than crude YP. Fifteen compounds in YP were identified, and crocins were the predominant compounds. The cell experiment results showed that YP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner. Moreover, YP also inhibited HepG2 cells in G2/M stage, increased the level of intracellular reactive oxygen species (ROS), and enhanced cell apoptosis. Real-time quantitative polymerase chain reaction (RT-PCR) analysis revealed the up-regulation of caspase-3, 8, 9, and bax and down-regulation of bcl-2 in HepG2 cells. Overall, these findings suggested that YP had potential cytotoxic activity in HepG2 cells by induction of apoptosis, which might be beneficial to human health.
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Evolution of the antioxidant capacity and phenolic contents of persimmon during fermentation
Bo Zou,Jijun Wu,Yuanshan Yu,Gengsheng Xiao,Yujuan Xu 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
The changes in antioxidant capacity and phenolics of persimmon during alcoholic fermentation, acetification, and short aging were investigated. An increase in the antioxidant activity was observed when persimmon was transformed from puree to vinegar. The total content of phenolics remained stable, in contrast to the concentration of condensed tannin, which significantly (p\0.05) increased during alcoholic and acetic fermentations, although followed by a decrease after aging. The phenolic compounds were characterized and quantitated. Gallic acid was the main phenolic compound, and its content increased by 14.4% during alcoholic fermentation and reduced by 53.5% during acetic fermentation. Additionally, the flavan- 3-ol compounds increased during alcoholic fermentation and acetification. Vanillyl alcohol, (-)-epigallocatechin, and p-coumaric acid were not observed in persimmon puree but detected in persimmon wine and vinegar. These results indicate that alcoholic and acetic fermentation can improve the antioxidant capacity of persimmon fruit.
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WenJuan Yan,YingYing Cai,Qun Zhang,YuSi Liu,WenChun Xu,YiBing Yin,YuJuan He,Hong Wang,XueMei Zhang 한국미생물학회 2013 The journal of microbiology Vol.51 No.4
Hsp100/Clp proteins have crucial functions in the protein quality control, stress tolerance, and virulence of many pathogenic bacteria. ClpE is an important virulence factor involved in adherence and invasion in Streptococcus pneumoniae. To explore the underlying mechanism, we screened ClpE interaction proteins using a bacterial two-hybrid system and co-immunoprecipitation. We used ClpE as bait and constructed the pBT-ClpE bait plasmid for two-hybrid screening. Then, we constructed ClpE::GFP fusion for co-immunoprecipitation screening using anti-GFP monoclonal antibody. We obtained eight potential ClpE interaction proteins,including carbamoyl-phosphate synthase, pyruvate oxidase (SpxB), phosphoenolpyruvate-protein phosphotransferase,aminopeptidase N (pepN), L-lactate dehydrogenase, ribosomal protein S4, sensor histidine kinase (SPD_2019), and FtsW (a cell division protein). FtsW, SpxB, pepN, and SPD_2019 were confirmed to interact with ClpE using Bacterial Two-hybrid or Co-immunoprecipitation. Morphologic observations found that ΔclpE strain existed in abnormal division. β-Galactosidase activity assay suggested that ClpE contributed to the degradation of FtsW. Furthermore, FtsW could be induced by heat shock. The results suggested that ClpE might affect cell division by regulating the level of FtsW. These data may provide new insights in studying the role of ClpE in S. pneumoniae.
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