Piezo2: A Candidate Biomarker for Visceral Hypersensitivity in Irritable Bowel Syndrome?

( Tao Bai ),( Ying Li ),( Jing Xia ),( Yudong Jiang ),( Lei Zhang ),( Huan Wang ),( Wei Qian ),( Jun Song ),( Xiaohua Hou ) 대한소화기기능성질환·운동학회 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.3

Background/Aims Currently, there exists no biomarker for visceral hypersensitivity in irritable bowel syndrome (IBS). Piezo proteins have been proven to play an important role in the mechanical stimulation to induce visceral pain in other tissues and may also be a biomarker candidate. The aim of this study was to test the expressions of Piezo1 and Piezo2 proteins in the intestinal epithelial cells from different intestinal segments and to explore the correlation between Piezo proteins expression and visceral pain threshold. Methods Post-infectious IBS was induced in mice via a Trichinella spiralis infection. Visceral sensitivity was measured with abdominal withdrawal reflex to colorectal distention. Inflammation in the small intestine and colon was scored with H&E staining. Expression location of Piezo proteins was confirmed by immunohistochemistry. Abundance of Piezo proteins were measured with real-time reverse transcriptase polymerase chain reaction. Results Piezo1 and Piezo2 proteins were expressed in the intestinal epithelial cells. The expression levels of Piezo1 and Piezo2 were abundant in the colon than the small intestine (P < 0.001 for Piezo1, P = 0.003 for Piezo2). Expression of Piezo2 in the colon significantly correlated to the visceral sensitivity (r = -0.718, P = 0.001) rather than the mucosal inflammation. Conclusion Piezo2 is a candidate biomarker for visceral hypersensitivity in IBS. (J Neurogastroenterol Motil 2017;23:453-463)

Analysis on the Characteristics and Prognosis of Pulmonary Neuroendocrine Tumors

Wu, Bai-Shou,Hu, Yi,Sun, Jing,Wang, Jin-Liang,Wang, Peng,Dong, Wei-Wei,Tao, Hai-Tao,Gao, Wen-Juan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Objective: To retrospectively review the clinical characteristics and analyze the prognostic factors of Chinese patients with pulmonary neuroendocrine tumors. Materials and Methods: The clinical data of 176 patients with pulmonary neuroendocrine tumors in Chinese PLA General Hospital from Mar., 2000 to Oct., 2012 were retrospectively analyzed. The parameters were evaluated by univariate and multivariate analysis, including the gender, age, smoking history, family history, TNM staging, localization (central or peripheral), tumor size, nodal status, histological subtype and treatment (operation or non-operation). Results: There were 23 patients with typical carcinoids (TC) (13.1%), 41 with atypical carcinoids (AC) (23.3%), 10 with large cell neuroendocrine carcinoma (LCNEC) (5.7%) and 102 with small cell lung cancer (SCLC) (57.9%). The median follow-up time was 64.5 months for AC, 38 months for LCNEC and 27 months for SCLC. The typical carcinoid censored data was 18 (more than 50% of the patients), so the median follow-up time was not obtained, and actuarial 5-year survivals for TC, AC, LCNEC and SCLC were 75.1%, 51.7%, 26.7% and 38.8%, respectively. COX univariate analysis revealed that the age (P=0.001), histological subtype (P=0.005), nodal status (P=0.000), treatment (P=0.000) and TNM staging (P=0.000) were the prognostic factors of the patients with pulmonary neuroendocrine tumors, whereas its multivariate analysis showed that only the age(P=0.001), TNM staging (P=0.002) and treatment (P=0.000) were independent prognostic factors. Conclusions: Radical surgery remains the treatment of choice, and is the only curative option. The age, TNM staging and treatment are confirmed to be the independent prognostic factors in multivariable models for pulmonary neuroendocrine tumors.

黄士陵篆刻艺术特征探赜

白洋涛,(Bai, yang-tao) 한국서예학회 2021 서예학연구 Vol.39 No.-

최근 몇 년 동안 황사릉(黃士陵, 1849-1908) 전각예술의 영향력이 날이 갈수록 커지면서 황사릉 전각예술과 그 유파 인장을 더욱 많은 전각가들이 받아들이게 되었고 결국 무시할 수 없는 인단(印壇)의 역량(力量)이 되었다. 본 논문은 황사릉의 전각작품을 기법측면, 예를 들어 도필(刀筆) 형태 표현, 내외공간의 안배, 인장에서 드러내는 효과 등을 체계적으로 분석하였다. 또한 황사릉 전각예술의 특징에 대해 거시적이며 전반적인 파악과 미시적이며 심도 있는 고찰을 했다. 아울러 황사릉과 동시대 그리고 조금 늦은 시기의 전각가를 횡향(橫向)과 종향(縱向) 두 측면에서 비교해 황사릉 전각예술의 객관적인 특징을 도출하였다. 마지막으로 본 논문이 앞으로의 황사릉 전각과 그 유파 연구에 보탬이 됐으면 한다. In recent years, with the increasing influence of Huang Shiling s seal cutting art, Huang Shiling s seal cutting art and its genre seals have been increasingly accepted by more Indians and become a force that cannot be ignored. Based on the systematic analysis of Huang Shiling’s seal cutting works, this paper analyzes the performance of Huang Shiling’s seal cutting works in terms of knife and pen morphology, internal and external space arrangement, and the presentation effect of printing. In-depth investigation of Huang Shiling s seal cutting works from the microscopic aspect, and comparing Huang Shiling with the seal carving masters of the same period and later seal carving masters from both horizontal and vertical aspects, and draws a more objective conclusion about the artistic characteristics of Huang Shiling s seal cutting. , I hope to contribute to the study of Huang Shiling s seal cutting and its genre seals, and it will be helpful. 近年来,随着黄士陵篆刻艺术影响力的日渐增强,黄士陵篆刻艺术及其流派印,愈加被更多的印人所接受,成为一项不可忽视的印坛力量。本文通过对黄士陵篆刻作品技法层面的系统分析,对黄士陵篆刻作品在刀笔形态表现、内外空间安排、印作呈现效果等方面进行分析,既包含宏观方面对黄士陵篆刻艺术特征的整体把控,又包含微观方面对黄士陵篆刻作品进行的深入考察,并从横向与纵向两个方面对黄士陵以及与其同时期的篆刻名家、比之稍晚的篆刻名家做比较,得出较为客观的黄士陵篆刻艺术特征的结论,希冀能够对黄士陵篆刻及其流派印的研究添砖加瓦,有所助益。

가정용 LED 조명 기구의 디자인 연구 - 조형적 특징을 중심으로

백박도 ( Bo Tao Bai ),김철수 ( Chul Soo Kim ) 한국기초조형학회 2013 기초조형학연구 Vol.14 No.5

LED 제조 기술은 급속도로 발전하였지만 조명 기구 디자인은 그 뒤를 따랐다. 조명 기구는 상품 디자인상에서 대체적으로 원래의 형태는 그대로 유지한 채 단지 전통 조명 기구안의 광원만 LED로 대체하였다. LED 광원의 독특한 성능은 조명 기구의 조형 디자인에 변화를 제공하고 있다. 본 논문은 Kiss의 연구논문 검색을 활용하여 조형특징, 조형요소, 조명의 Keyword를 검색하여 조사된 조형에 관한 연구논문 중 본 연구 주제와 관련이 있는 30개 선행연구 논문을 선정하여 조형의 특징에 관한 분석방법을 조사하였다. 본 연구의 대상인 조명 제품은 매우 광범위 하며, 연구내용이 디자인의 조형성을 다루고자 하기에 굿 디자인으로 인정되는 제품을 연구대상의 범위로 한다. 이에 조명 디자인의 표본은 세계적으로 Good Design 공인된 RED DOT(표본 110개), IF(표본 17개), IDEA (표본 19개) 3 대 국제공모전상 3년 건(2009년-2011년도)의 수상작을 연구의 대상으로 선택하였다. Image Mapping 방법을 통해 형태, 색깔, 재료 의 3개 방면으로부터 가정용 일반 조명기구와 LED조명기구에 대해 연구한다. 이어서 일반 조명기구와 LED 조명기구의 조형적 특징을 근거로 미래 조명 기구의 조형 디자인에 대한 발전 추이를 예측할 수 있다. 첫째: 심플하고 이성적인 조형. 구조적으로 다양한 기능성을 지니는 디자인이 증가될 것이다. 둘째: 특이한 조형을 자유롭게 시도된 디자인이 다양화될 것이다. 셋째: 다양한 문화 현상과 친환경적인 이념을 중시하고 자연적 요소를 활용한 디자인이 증가될 것이다. 따라서 얻어낸 결론과 결합하여 가정용LED조명 기구에 대하여 개념 구색 디자인을 진행한다. Along with the high speed development of the LED lighting technology, lamps and lanterns design follows with high gear, too. Currently, LED lamps design is more like a photo source replacement by LED, keeping the basics of the original shapes of the lamps and lanterns. Still, LED photo source shows its special characteristics. This essay, by selecting the key words “styling characteristics, styling basics, lighting” from the KISS Essay Web site, analyzes the study and research methods of 30 papers related from the web site. There are many products for lamps and lanterns. Those with better and special design are chosen for the object of study. Lamps rewarded by the world three product design institutes are used in this essay, RED DOT(110 samples), IF(17 samples), IDEA(19 samples). The Image Mapping is used to analyze the shape, color and material of the samples. Based on the styling characteristics of both the LED and conventional lamps, forecast the future styling design of the LED lamps as well as its development trend. Trend 1: styling features are more simple and rational. Styling design is more focus on function. Trend 2: different and unique shapes are freely adopted. Decorative purpose is addressed more. Trend 3: lamps design addresses multi-culture theme and environment friendly concept. People see more and more features reflecting nature in the styling design of the lamps. Last, based on the conclusion, start the styling design of home use LED lamps.

Normal Estimation for Mass Point Clouds of Irregular Model in the 3D Reconstruction based on Fuzzy Inference

Liu Yan-ju,Jiang Jin-gang,Miao Feng-juan,Tao Bai-rui,Zhang Hong-lie 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.5

This paper presents a fuzzy normal estimate for mass point clouds of irregular models in reconstruction. The irregular model is complex object that some part is smooth and some parts are irregular including sharp features. Therefore, we put kNN and curvature of mass point clouds to fuzzy inference system to divide the kind of point clouds and the output of FIS can determine which part of tooth point clouds belong to. For different kinds point clouds, corresponding algorithm is given. Point clouds in the smooth area are estimated normal by PCA directly and ones in other regions of thin or sharp area are estimated by checker and attach points. This method is simpler than those complex methods used on the whole point clouds directly. The experiment results show that much time is saved and surface reconstruction is very fine than PCA and WLOP.

Folate Deficiency and FHIT Hypermethylation and HPV 16 Infection Promote Cervical Cancerization

Bai, Li-Xia,Wang, Jin-Tao,Ding, Ling,Jiang, Shi-Wen,Kang, Hui-Jie,Gao, Chen-Fei,Chen, Xiao,Chen, Chen,Zhou, Qin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Fragile histidine triad (FHIT) is a suppressor gene related to cervical cancer through CpG island hypermethylation. Folate is a water-soluble B-vitamin and an important cofactor in one-carbon metabolism. It may play an essential role in cervical lesions through effects on DNA methylation. The purpose of this study was to observe effects of folate and FHIT methylation and HPV 16 on cervical cancer progression. In this study, DNA methylation of FHIT, serum folate level and HPV16 status were measured using methylation-specific polymerase chain reaction (MSP), radioimmunoassay (RIA) and polymerase chain reaction (PCR), respectively, in 310 women with a diagnosis of normal cervix (NC, n=109), cervical intraepithelial neoplasia (CIN, n=101) and squamous cell carcinoma of the cervix (SCC, n=101). There were significant differences in HPV16 status (${\chi}^2=36.64$, P<0.001), CpG island methylation of FHIT (${\chi}^2=71.31$, P<0.001) and serum folate level (F=4.57, P=0.011) across the cervical histologic groups. Interaction analysis showed that the ORs only with FHIT methylation (OR=11.47) or only with HPV 16 positive (OR=4.63) or with serum folate level lower than 3.19ng/ml (OR=1.68) in SCC group were all higher than the control status of HPV 16 negative and FHIT unmethylation and serum folate level more than 3.19ng/ml (OR=1). The ORs only with HPV 16 positive (OR=2.58) or with serum folate level lower than 3.19ng/ml (OR=1.28) in CIN group were all higher than the control status, but the OR only with FHIT methylation (OR=0.53) in CIN group was lower than the control status. HPV 16 positivity was associated with a 7.60-fold increased risk of SCC with folate deficiency and with a 1.84-fold increased risk of CIN. The patients with FHIT methylation and folate deficiency or with FHIT methylation and HPV 16 positive were SCC or CIN, and the patients with HPV 16 positive and FHIT methylation and folate deficiency were all SCC. In conclusion, HPV 16 infection, FHIT methylation and folate deficiency might promote cervical cancer progression. This suggests that FHIT may be an effective target for prevention and treatment of cervical cancer.

Differential Protein Expression Profiling in Pleurotus ferulae Mycelium Caused by Asafoetida Extracts Using a Proteomics Approach

Bai, Yujia,Huang, Wenshu,Tao, Yongxia,Feng, Zuoshan The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1

The precise mechanism by which asafoetida extract influenced the growth of Pleurotus ferulae mycelium was investigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group, the two asafoetida extracts played a significant role in promoting the growth of P. ferulae. Two-dimensional electrophoresis and LC-MS/MS were performed to examine changes in the expression of proteins in the mycelium of P. ferulae. A total of eight differentially expressed proteins were identified, including a heat shock protein, a flavin-containing monooxygenase, and a NADPH: quinone oxidoreductase type IV, as well as hypothetical and unknown proteins. Expression change of these proteins confirmed that asafoetida extracts significantly affected P. ferulae growth, metabolism, and secondary product metabolism. These results provide new insights into biochemical mechanisms underlying the relationship between P. ferulae and asafoetida.

Facile synthesis of AgPt nano-pompons for efficient methanol oxidation: Morphology control and DFT study on stability enhancement

Tao Shao,Dehong Bai,Mingzhu Qiu,Yu Li,Qiankun Zhang,Ziyu Xue,Shijie He,Dongxia Zhang,Xibin Zhou 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-

Facile synthesis of more dendritic and uniform Pt-based nanostructures with carbon materials couldgreatly reduce cost and increase Pt utilization for methanol oxidation reaction (MOR) in direct methanolfuel cell (DMFC). This study reports a novel one-pot method to fabricate AgPt nano-pompons (AgPt NPs)with the guidance of N-GQDs through AA reduce the precursor of Ag and Pt. Morphology characterizationdescribes N-GQDs as morphology guiding and dispersing agents to regulate the dendrite formation ofnano-pompons. Under the optimized conditions, the AgPt NPs (Ag1Pt2) display above 11 times improvementin electrocatalytic activity and higher stability for the MOR compared with Pt/C catalysts. Densityfunction theory (DFT) studies prove that the introduction of Ag can greatly enhance the adsorption of COon Pt and promote the transformation of CO to COOH. The facile synthetic method and excellent MORperformance endow AgPt NPs with great application prospect in DMFCs as an anode catalyst.

Down-regulation of FRα Inhibits Proliferation and Promotes Apoptosis of Cervical Cancer Cells in Vitro

Bai, Li-Xia,Ding, Ling,Jiang, Shi-Wen,Kang, Hui-Jie,Gao, Chen-Fei,Chen, Chen,Zhou, Qin,Wang, Jin-Tao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Folate receptor alpha ($FR{\alpha}$) mediates folate uptake by endocytosis, and while folate is essential to DNA methylation and synthesis and may have an important role in proliferating cells. $FR{\alpha}$ is known to be expressed in rapidly proliferating cells, including many cancer cell lines, but there has been no systematic assessment of expression in cervical cancer cell lines. The aim of the present study was to evaluate the effects of $FR{\alpha}$ on proliferation and apoptosis of cervical cells and correlation mechanism. In this study, we investigated the biological function of $FR{\alpha}$ in Hela cells using RNA interference. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK8) assay, while cell cycling and apoptosis were assessed by flow cytometry, mRNA levels by real time-PCR and protein levels of $FR{\alpha}$, c-Fos and c-Jun by Western blotting. The results revealed that $FR{\alpha}$ was highly expressed in Hela cells and its silencing with a small interfering RNA (siRNA) inhibited cell proliferation and induced cell apoptosis, arresting the cell cycle in G0/G1 stages while decreasing the proportion in S and G2/M stages, and suppressed the expression levels of c-Fos and c-Jun. In conclusion, the results of this study indicated that $FR{\alpha}$ down-regulation might be capable of suppressing cervical cancer cell proliferation and promoting apoptosis. It suggested that $FR{\alpha}$ might be a novel therapeutic target for cervical cancer.

Correlations Between Fasciology and Yin Yang Doctrine

Hui Tao,Mei-chun Yu,Hui-ying Yang,Rong-mei Qu,Chun Yang,Xin Zhou,Yu Bai,Jing-peng Wu,Jun Wang,Ou Sha,Lin Yuan 사단법인약침학회 2011 Journal of Acupuncture & Meridian Studies Vol.4 No.2

The aim of this study is to explore the correlations between fasciology and yin yang doctrine. Professor Yuan developed fasciology by three-dimensional reconstruction of connective tissue (fascia) in the trunk and limbs of the human body and tracing back to tissue origins in light of biological evolution and developmental biology. Fasciology states that the human body can be divided into two systems: the supporting-storing system and the functional system. This article elaborates on the roles of the two systems and their mutual relationship. The two systems are used to analyze the yin,the yang, and their relationship. The two systems are promoted but also restricted in different contexts. The supporting-storing system is formed by undifferentiated connective tissue and provides undifferentiated cells and nutrients for differentiated cells of the functional system. Thus, the supporting-storing system could be classified as quiet, similar to yin. The functional system continuously maintains the various functional activities of the human body. Thus, the functional system could be classified as active, similar to yang. In interpreting the yin yang doctrine from the point of view of fasciology, yin can be compared with the supporting-storing system and yang can be compared with the functional system.