Bacillus thuringiensis Y M 系 phage의 生理的 性狀에 관한 硏究

李泰雨,安慶濬 西原大學校 1984 西原大學 論文集 Vol.13 No.-

Bacillus thuringiensis YM-series phage의 生理的 特性은 다음과 같다. 1. YM-phage 1, 2, 3은 높은 宿主特異性을 나타내어 Bacillus thuringiensisS-1 菌株에만 宿主域이 限定되어 있었다. 2. YM-phage 1, 2, 3의 pH 安定性은 pH6에서 pH8까지의 범위 내에서 安定하였다. 3. YM-phage 1, 2, 3은 L-broth내에서 60°∼70℃에서 급격히 不活性化되었다. 4. YM-phage 1, 2, 3은 L-broth에서 상당히 安定하였으며 0.05 M 인산완충액 , 0.002 M 인산완충액, 0.02M Tris 완충액에서도 비교적 安定하였고 physiological salt solution에서는 不安定하였다. 5. Bacillus thuringiensis는 phage의 感染 後에 일정시간 지난 후 溶菌되었다가 耐性菌이 增殖하는 樣狀을 보였다. Physiological characteristics of YM-series phages of Bacillus thuringiensis were studied. The results were as follows. 1. The YM-phage 1, 2 and 3 were highly specific for Bacillus thuringiensis S-1 strain. 2. The YM-phage 1, 2 and 3 were stable at the range of pH6 and pH8 in L-broth. 3. The YM-phage 1, 2 and 3 were completely inactivated from 60℃ to70℃ in L-broth. 4. The YM-phage 1, 2 and 3 were completely stable in L-broth. But, in 0.05M and 0.002M phosphate buffer and 0.02M Tris buffer, they were less stable than in L-broth and very unstable in physiological salt solution. 5. The Bacillus thuringiensis were lysed after phage infection and then resistant strains were grown after one or two hours.

한국 문학에 나타난 이별의 미학 : 특히 고대소설을 중심하여

金泰俊 명지어문학회 1979 明知語文學 Vol.11 No.-

151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

靑鶴洞傳說과 神仙思想

김태준 명지대학교 1976 明大論文集 Vol.9 No.-

세포학적인 검사를 통해 자궁경부암의 조기 발견과 합리적인 병원내 산부인과의 운영 체계에 관한 고찰

김태전,배형준,김종열 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

In this study, the authers have investigated a true appreciation on the importance and the actual conditions of utilization of cytologic examination in gynecologic services with 3 step, that is, in 1 step the importance of cytologic examination in gynecologic services has been investigated from the results of 4,024 female of cytologic examination, in 2 step the actual conditions of utilization of cytologic examination have been investigated from 60 medical institutes through questionaire, and in 3 step the more detailed subjects of cytologic examination in gynecologic services have been investigated from 3 medical institutes selected randomly, such an investigations were done to make the programs of improvement in order to promote the activation of gynecologic medical services that shall be able to decrease occurrence of uterine cervical cancer, which is still the most prevalent cancer in korean female, and to make those on effective diagnosis and treatment activaton that shall be able to increase the income of medical institutes. The programs of improvement according to conclusions obtained from investigations like these were summarized as the following. 1. Program to activate positively cytologic examination It was well known that good correlation was noted between number of cytologic examination and those of histologic examination, those of gynecology procedures, and those of gynecologic surgeries. But it was a fact that a most of gynecology board certified physicians and physicians, and medical institutes have not utilized actively cytologic examination in gynecologic practice. Therefore all of married woman should be had politically cytologic examination at regular intervals in order to prevent and detect early uterine cervical cancer. 2. Program to establish exclusively responsible managed systems Managed systems that are exclusively responsible for cytologic examination should be established in order to have examination at regular intervals exactly(six months). 3. Program to utilize correctly the current medical insurance regulations about medical treatment charges The medical charges of cytologic exmination should be applied correctly to the current medical insurance regulations about medical treatment charges in order to increase the income of medical institutes. Because the medical charges of cytologic examination for the levels of medical check up and preventive medicin exception cancer or clinically suspicious cancer diagnosed by physicians is applicable to general medical treatment charges under the current medical insurance regulations. 4. Program to utilize reasonable diagnostic system in cytologic examination. Since certificated technologist screen first thing many microscopic slides for cytologic examination, lastly gynecologic board should make a final diagnosis on only abnormal slides. It is reasonable diagnostic system that can observe more microscopic slides, decrease misdiagnosis and high personnel expenses, and increase income in cytologic examination. Consequently it is necerssary to establish reasonable diagnostic system. 5. Program in order to promote the efficiency of the following examination and treatment system for patients to whom abnormal cell were detected. The following examination and treatment system that were corresponded with diagnosis, and automated and standardized system from receipt to gynecologic practice should be established to offer patients ease and trust, and also medical institutes the more income through the activation of gynecologic practice. Finally, the authers think that it is very important that cytologic examination should be included in routine gynecologic practice.

鶴峯 金誠一의 日本日錄

金泰俊 명지어문학회 1976 明知語文學 Vol.8 No.-

日本 新儒學의 成立과 朝鮮學者 : 壬亂前後의 朝鮮文化의 對日影響을 중심하여

金泰俊 명지대학교 1975 明大論文集 Vol.8 No.-

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.

Bacillus thuringiensis의 autolysis에 影響을 미치는 요인에 관한 硏究

李泰雨,安慶濬 西原大學校 1984 西原大學 論文集 Vol.13 No.-

Bacillus thuringiensis S-1 菌에서 osmotic shock를 加하여 效率的으로 autolysis를 일으키기 위한 諸般 要因의 效果를 조사하였다. 1. 1M의 1價이온을 처리했을 때 가장 autolysis가 잘 일어났다. 2. 振탕(?)培養을 했을 때가 停置培養했을 때보다 더 autolysis가 잘 일어났다. 3. pH 9에서 45℃로 培養했을 때가 가장 빠른 autolysis를 보였다. 4. 對數增殖期의 細菌에서 autolysis가 가장 왕성하였다. 5. autolysis가 진행되는 과정에서 2가 이온을 첨가하면 autolysis가 억제되었다. The effect of several factors of autolysis were studied in order to induce autolysis effectly by osmotic shocks on Bacillus thuringiensis S-1 strain. 1. 1M of univalent cation induced autolysis most effectively. 2. Shaking incubation was more effective than without shaking. 3. The most rapid autolysis was induced at pH 9 and in 45℃. 4. The most vigorous autolysis was induced in the cells of exponential growth phase. 5. In the process of autolysis the addition of bivalent cation inhibited autolysis immediately.

HUMACTBP2 대립 유전자의 새로운 명명법

구태완,이용욱,한길로,황적준 大韓法醫學會 1998 대한법의학회지 Vol.22 No.2

Allele designation for HumACTBP2 is not yet established while many authors introduced different kinds of designation methods. Here, we are introducing a new allele designation method. We used allelic ladder as an internal size standard on behalf of GS-500ROX and designation of each allele was followed recommendation of DNA commission of International Society of Forensic Hematogenetics(ISFH). This method is considered more reasonable for complex repeat loci like HumACTBP2 than other methods that published before.