Phytol과 들미나리추출물이 Sarcoma 180마우스의 T Subset에 미치는 효과

류태형,박건영,장명웅,이숙희,선우양일,김광혁 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

본 연구는 녹황색채소류에서 추출되어 항암효과를 나타내는 활성물질로 보고 되어 있는 phytol과 들미나리추출물을 sarcoma 180마우스에 주사한 후 적출한 비장세포내 T 임프구와 T subset, 그리고 asialo ?? 세포를 정량하여 다음과 같은 결과를 얻었다. 1) 종양 마우스에 phytol을 투여하였을 때 비장세포내의 T cell과 T-subset은 종양세포이식에 의하여 상승된 치를 더욱 증가시켰다. 그러나 들미나리추출물의 경우는 대동소이하였다. 2) Asialo ?? 세포는 종양마우스에 phytol이나 들미나리추출물을 주사하였을 때 모두 상승하였으며 정상마우스에 phytol을 투여하였을 때도 대조군에 비하여 상승했지만 들미나리추출물을 작용시켰을 때는 저하되었다. 3) ??/?? 세포비는 종양마우스에 phytol을 주사하였을 때 감소를 보였지만 정상 마우스에 투여하였을 때는 더욱 크게 낮아졌다. 그러나 들미나리추출물을 정상마우스에 투여하였을때는 크게 감소하던 것이 종양마우스에 적요 시켰을때는 증가현상을 보였다. 이상의 결과로 미루어 볼 때 phytol이나 들미나리추출물은 종양마우스에서 작용자세포인 자연살해세포(natural killer cell)의 활성인자로서 작용할 가능성이 높을 것으로 사료된다. Several studies have shown that phytol isolated from perilla leaf and small water dropwort(Oenanthe javanica(BL)D.C, wild type)extract reveal antitumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antitumor activity of these substances. The results obtained from the experiment were as follows : When phytol was injected into the sarcoma-180 transplanted mice(control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo ?? cells were increased when phytol or small water dropwort extract was injected into tumor-transplanted mice. In normal mice the number of asialo ?? cells increased with phytol injection and decreased with small water dropwort extract injection. ??/?? cell ratios were decreased when phytol was injected into tumor-transplanted mice, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract can activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

Effects of Myo-inositol and Riboflavin on Ethanol-Induced Fatty Liver and Hyperlipidemia in Rats

Rhew, Tae Hyong,Chun, Jang Soo 부산대학교 1987 자연과학논문집 Vol.44 No.-

sz12/ Ethanol이 지방대사에 미치는 영향중에서 가장 특징적인 것이 과유지방혈증과 지방간에서부터 간경변에 이르는 간의 증상들인바, 본 실험에서는 diet 총 열량의 36%에 해당되는 ethanol을 실험용 흰쥐에 45일간 투여해서 ethanol유발성 지방간과 과유지방혈증을 일으키고 myo-inositol과 riboflavin의 처리에 따른 그 치료효과를 고찰하였다. Ethanol diet에 2.5mg/100g body wt.의 myo-inositol을 처리한 흰쥐에서는 간과 plasma에서 모든 종류의 지질들이 현저히 감소하였고, riboflavin(6.6mg/200g body wt.)을 처리한 쥐에서는 단지 간에서 Total lipid, 중성지질, 인지질 그리고 콜레스테롤의 양만이 현저히 감소되었다. The lipid-lowering effect of Myo-inositol and Riboflavin was examined in male Sprague-Dawley rats fed ethanol as 36% of the total calories for a duration of 45day, Ethanol produced typical hepatic steatiosis and hyperlipidemia characterized by significantly elevated levels of total lipid, triglycerides, phospholipid, and free fatty acids. Supplementation of ethanol diet with riboflabin(6.6mg/100g body weight) significantly lowered ethanol-induced increases of the various lipid classes such as total lipid, triglycerides, anf total cholesterol in liver, but reduction in plasma lipid classes was minimal. The effect of Myo-inositol appeared to be superior to that of riboflabin. Myo-inositol significantly reduced ethanol-induced elevation of all classes of lipids in livers and plasma.

Particle Processing of Organic Minerals

( Tae Hyong Rhew ) 자연사 미래환경학회(구 자연사환경학회) 2006 자연사 미래환경학회 학술발표논문집 Vol.2006 No.-

Depletion of Extra-Hepatic Tissue Carnitine : A Source of Increased Hepatic Carnitine in Chronic Alcoholid Rat Chronic Alcoholic Rat에 있어서 Hepatic Carnintine 증가의 원인

Rhew, Tae Hyong 부산대학교 1990 자연과학논문집 Vol.49 No.-

Male Sprague-Dawley rats were fed nonacoholic or alcoholic diets as such or supplemented with carnitine or its precursors for 56 days. Blood and tissues were collected and analyzed for carnitine and acylcarnitine by a radioisotopic procedure. While carnitine concetrations were higher than normal in alcohol compromised liver, they were consistently lower than normal in most of the extrahepatic tissues. The most significant carnitine depletion was found in the skeletal and cardiac muscle. All tissues were repleted by supplementary carnitine, however, carnitine precursors were not so efficacious. It is concluded that chronic alcoholic liver sequestered carnitine at the expense of extrahepatic tissues. Indexing key Words: Chronic alcoholism, tissue-carnitine, acylcarnitine, supplementary carnitine, carnitine depletion, carnitine repletion, carnitine precursors. Caritine is essential for the translocation of fatty acids among organs and organelles(1). Its supply in healthy mammals is ensured by endogenous biosynthesis from lysine and methionine as well as via ditary intake(2). In the rat, carnitine is biosynthesized primarily in the liver and distributed to other organs(3). Liver carnitine concentrations have been shown to increase in a number of pathophysological conditions such as starvation(4,5), diabetes mellitus(6) and clofibrate treatment(7). In addition an increase in hepatic carnitine concentration has been observed in chronic alcoholic rats(8-11): however, the mechanism of its accumulation has not been elucidated. The evidence is presented to support the hypothesis that increased carnitine concetraations in an ethanol-compromised liver may have resulted from sequestration of carnitine from plasma at the expense of extrahepatic tissues.

Physiolosical Effects of Physical Exercise in Rats

Rhew, Tae Hyong,Chun, Jang Soo 부산대학교 1986 자연과학논문집 Vol.42 No.-

Plasma 및 중요 organ들에 있어서의 각 종류의 지질과 단백질 대사에 대한 근육운동의 영향에 대해서 고찰하였다. 흰쥐를 25℃의 수조에서 35분간 수영시켜서 10주 후에 plasma와 중요 조직들을 채취하였다. 정량분석한 결과, test group을 reference group에 비교할 때에 단백질에는 차이가 없었으나 lipid profile (total lipid, triglyceride, and free fatty acid)의 양은 주로 plasma와 근육조직, 간, 지방조직에서 현저히 줄어들었다. cholesterol, esterified cholesterol과 total cholesterol은 모든 조직에서 운동에 의하여 감소되었고 이와 반대로 오직 phospholipid 만은 근육과 신장을 제외한 모든 조직에서 운동에 의하여 증가하였다. 이러한 각 조직에서의 lipids의 감소는 근육운동시에 지질이 β-산화를 통하여 에너지원천으로 사용되고 있음을 시사하는 것이다. The effect of physical exercise on the contents of various lipid classes and protein were examined in female sprague-Dawley rats fed nutritionaly adequate solid diets for a duration of 10 weeks. The exercising group animals were forced to swim at a water bath at 25℃ for 35 minute daily. Physical activities produced significant reduction in the levels of TG and FFA in plasma and various organs. Physical esercise significntly reduced the levels of chloesterol esters, and total chloesterol in plasma and the orgasn. However, phospholipid values were elevated in plasma and all the organs except skeletal muscle and heart in exercise group animals. Physical execise did not affect protein values in the ortans but significantly reduced those in plasma. Thus, it is concluded that in rats, physical exercise significantly lowered the lipid contents especially TG, FFA, and cholesterols in plasma and various organs due to oxidation of fats to meet energy demand in muscle activity. Evidently, moderate physical exercise is valuable for health, especially, with respect to cholisterol significance.

식품, 영양과 암의 관계

류태형(Tae-Hyong Rhew) 한국식품영양과학회 1985 한국식품영양과학회지 Vol.14 No.3

There is a trend that the total number of cancer cases is steadily increasing as the population grows. It has been estimated that 85% of the cancer rate in the U. S. is attributed to environmental factors. Among the enviromental factors, diet and nutrition appear to be related to the largest number of human cancers.<br/> Diet and nutrition might be related to cancer by several mechanisms. Food may contain a direct carcinogen or precursors that become carcinogens by spontanous reactions, or by host metabolism, or through the actions of microbial flora. Chemicals that cause cancers generally have reactive electrophilic centers which can combine with electron-rich atoms in nucleic acids and cause cancers by changing the genetic activity of the cells. A variety of factors in foods might be involved in the etiology of carcinogenesis.<br/> Chemicals in food that cause cancers include carcinogens of plants and animal origin and also those in drinking water. Other then these, fungal metabolites alcohol, asbestos, heavy metals, pesticides, and food additives might be included as food carcinogenesis. The method of cooking foods also might contribute to carcinogenesis. Some chemicals in foods act as promoters in carcinogenesis.<br/> Prevention of cancers by dietary practises have received much interest. Consumption of certain vegetables or cellulose can reduce carcinogenic activity of several compounds. A variety of antioxidants or micronutrients may be effective anticarciongens.<br/> Glutathione in the soluble fraction of the cells, is a major defense against oxidative and alkylating carcinogens. Recently anticarcinogenic activity of chlorophyll was demonstrated. Daily consumption of milk appears to effectively reduce stomach cancer.

생쥐에서 종양세포의 암유전자발현에 대한 울솔산의 효과

류태형(Tae Hyong Rhew),박성미(Sung Mi Park),박건영(Kun Young Park),정해영(Hae Young Chung),하재청(Jae Chung Hah),이정규(Chung Kyu Lee) 대한약학회 1992 약학회지 Vol.36 No.6

To investigate the effect of ursolic acid on the expression of oncogenes in tumor cells of mice, sarcoma 180 ascites tumor cells were implanted into the left groin of ICR mice and the tumor bearing mice were treated with ursolic acid. The expression of oncogenes were measured by in situ hybridization method. Ursolic acid significantly reduced the expression of oncogenes in the tumor cells. Therefore, it can be said that the prestated anticarcinogenic effect of ursolic acid could be partly ascribed to the mechanism included in the oncogene''s transcription level.

알콜 유발성 지방간에 대한 Carnitine의 DOSE-DEPENDENT 효과

류태형 부산대학교 1988 자연과학논문집 Vol.45 No.-

The objective of this study was to determine whether the lipotropic effect of supplementary D,L-carnitine was dose dependent. Male Sprague-Dawley rats were fed a liquid diet in which 36% of the total calories were substituted by ethanol for 45 days. The ethanol diet, containing naturally 1.27㎍ of L-carnitine per 1 gram of diet, was fed as such or was further supplemented with 0.4%, 0.9%, 1.2%, and 1.6%(w/w, dry weight basis) D,L-carnitine. In both plasma and liver, result showed a definite inverse relationship between the levels of lipids and those of carnitines. The concentrations of total lipids and triglycerides was significantly reduced with increasing levels of supplementary carnitine while those of carnitine were significantly elevated. The changes in the plasma and hepatic concentrations of various classes of lipid and carnitine were inversely correlated ans were appeared to be progressive up to 0.8% D,L-carnitine supplementation. Therefore, it was concluded that the lipotropic effect of dietary carnitine is dose-dependent, and that the optimal supplementary level is 0.8% D,L-carnitine.

생쥐에 이식된 암세포에 있어서의 Linoleic Acid의 암억제 효과

류태형,박성미,정해영,박건영,하재청 부산대학교 유전공학연구소 1992 분자생물학 연구보 Vol.8 No.-

ICR mice were transplanted with sarcoma 180 ascites tumor cells. Linoleic acid from Allium sativum for. Pekinase MAKINO was injected into the tumor bearing mice. Antitumor effect of the compound was investigated by using morphological and in situ hybridization means, Linoleic acid was effective in delaying the tumorigenesis process. However, the effects were appeared not to be associated with changes in selected oncogene expression.

Isolation and Characterization of four Carboxypeptidases in Canavalia lineata cotyledons

Yang, Jong Moon,Rhew, Tae Hyong,Koh, Suck Chan,Kwon, Young Myung 생화학분자생물학회 1979 BMB Reports Vol.28 No.5

Four carboxypeptidases, CP1, CP2, CP3, and CP4 were isolated from the cotyledons of germinating seedlings of Canaualia lineata by sequential chromatography on the following four columns : 1) CMcellulose, 2) Sephacryl S-300, 3) Procion red dye, and 4) Sephacryl S-200. A number of properties of the enzymes, such as substrate specificity, molecular weight, optimum pH, thermal stability, have been determined. Enzyme activities were measured using the Cbz(carbobenzoxy)-dipeptides containing phenylalanine at the penultimate position. The Km values of four carboxypeptidases for Cbz-Phe-Ala were 0.50, 0.65, 1.30, and 1.35 mM, respectively. The inhibition studies indicated that the four carboxypeptidases were all serine type. Each of the carboxypeptidases with molecular weights of 145, 114, 105, and 104 kDa, respectively, had the optimum enzyme activity at pH 5.0∼6.0. And they were sensitive to high temperature.