설편평세포암에서 CD44, MMP-2, MMP-9, TIMP-1의 발현

이상화,표성운,김영실,최목균 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Adhesion molecules and proteinases play an important role in the invasion and metastasis of a malignant tumor. However, there have not been many studies on tongue squamous cell carcinoma at Saint Mary's Hospital and Kangnam Saint Mary's Hospital, the Catholic University of Korea, between 1994 and 2000, and had not received chemo-or radiotherapy. The expression of the adhesion molecules CD44H, proteinases MMP-2 and MMP-9, and TIMP-1, which controls MMP activity, was analyzed by immunohistochemistry. In addition their association with the tumor size, lymph node metastasis, clinical stage, and cell differentiation was evaluated. Increased MMP-2 expression was significantly correlated with lymph node metastasis and clinical stage. However, it did not correlate with tumor size or cell differentiation. CD44H, MMP-9 and TIMP-1 expression were not significantly related to tumor size, lymph node metastasis, clincial stage and cell differentiation. The results suggest that MMP-2 is a possible immunohistochemical prognostic factor in tongue squamous cell carcinoma. Futher reasearches will be needed to investigate the additional tumor prognostic factors and their mechanisms.

액시드 캅셀(니자티딘 150 mg)에 대한 넥스 캅셀의 생물학적 동등성

김지만,이상봉,전성실,신영희 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

The purpose of the present study was to evaluate the bioequivalence of two nizatidine capsules, Axid (Lilly Korea Pharm. Co., Ltd.) and Nex (Bi-nex Pharm. Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The nizatidine release from the two nizatidine capsules in vitro was tested using KP Apparatus method with various dissolution media (pH 1.2, 4.0, 6.8 buffer solutions and water). The dissolution profiles of two nizatidine capsules were very similar at all dissolution media. Twenty four normal male volunters were divided into two groups with a randomized 22 cross-over study. After two capsules (300 ㎎ nizatidine) were orally administrated, blood as taken and the concentrations of nizatidine in serum were determined using HPLC with UV detector. The pharmacokinetic parameters such as AUC_(t), C_(max) T_(max) were determined. The result showed that the differences in AUC_(t), C_(max) and T_(max) between two nizatidine capsules absed on the Axid were-6.16%, -8.26% and -1.82%, respectively. There were no sequence effects between two capsules in these parameter. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25)(e.g., log(0.91) ∼log(0.97) and log(0.85)∼log(0.99) for AUC_(t) and C_(max), respectively), indicating that Nex capsule is bioequivalent to Axid capsule.

Effects of Tween 80 Pretreatment on Dry Matter Disappearance of Rice Straw and Cellulolytic Bacterial Adhesion

Lee, Chan Hee,Sung, Ha Guyn,Eslami, Moosa,Lee, Se Young,Song, Jae Y.,Lee, Sung Sill,Ha, Jong K. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9

An in situ experiment was conducted to find out whether Tween 80 improves rice straw digestion through increased adhesion of major fibrolytic bacteria. Rice straw was sprayed with various levels of Tween 80 non-ionic surfactant or SDS ionic surfactant 24 h before incubation in the rumen of Holstein steers. Dry matter (DM) disappearance and adhesion of F. succinogenes, R. flavefaciens and R. albus on rice straw after in situ incubation were measured by real-time PCR. Application of Tween 80 increased DM disappearance, which was more noticeable at an application level of 1% compared to lower application levels. Application of SDS resulted in an opposite response in DM disappearance with highest reduction in DM disappearance at 1% level. In a subsequent in situ experiment, higher Tween 80 was applied to rice straw in an attempt to find the optimum application level. Tween 80 at 2.5% gave better DM disappearance than 1% with a similar result at 5%. Therefore, an adhesion study was carried out using rice straw treated with 2.5% Tween 80. Our results indicated that Tween 80 reduced adhesion of all three major rumen fibrolytic bacteria to rice straw. Present data clearly show that improved DM disappearance by Tween 80 is not due to increased bacterial adhesion onto substrates.

오징어 부산물의 급여가 한우암소 산육 및 도체특성에 미치는 영향

Sang Min Lee(이상민),Sun Sik Jang(장선식),Seok Dong Lee(이석동),Seong Koo Hong(홍성구),Sung Sill Lee(이성실),Jae Seok Woo(우제석) 한국생명과학회 2012 생명과학회지 Vol.22 No.1

본 연구는 한우 암소의 산육 및 도체특성에 있어 오징어 부산물의 급여 효과를 알아보기 위해 수행되었다. 한우 16두(나이 7세, 체중484.1±63.6 ㎏)를 대조구 및 처리구(오징어 부산물 첨가), 등 2그룹(각각 8두)으로 완전임의 배치하였다. 사료급여는 두 그룹 모두 자유채식토록 하여 150일간 시험을 수행하였다. 일당증체량은 처리구가대조구 보다 유의적으로 높았다(p<0.05). 시험기간 내 사료섭취량 및 사료요구율은 처리구가 대조구 보다 유의적으로 낮았다(p<0.05). 육량특성에 있어서, 등지방두께, 등심단면적 및 육량지수는 두 처리구간 비슷한 경향을 나타내었고, 도체중은 처리구가 363.4 ㎏ 대조구 353.1 ㎏으로 처리구간 유의적 차이는 없었다. 육질특성에서는 육색, 지방색, 조직감 및 성숙도는 두 그룹간 유의적 차이가 없었다. 그러나 근내지방도 및 육질 1등급 이상 출현율은 대조구 보다 처리구가 높았다. 그러므로 본 연구의 결과 오징어부산물의 급여는 한우 암소의 산육 및 도체특성을 향상시키는 것으로 생각된다. This study was conducted to evaluate the effect of feeding squid by-products on the growth performance and carcass characteristics of Hanwoo cows. Sixteen Hanwoo cows, aged 7 years and weighing 484.1±63.6 ㎏, were randomly allocated to 2 feeding groups (8 animals per group): control and treatment (fed squid by-products). Both groups were fed ad libitum for 150d. Average daily gain was significantly higher in the treatment group than in the control group (p<0.05). During the experimental period, DMI and feed conversion ratios were significantly lower in the treatment group than in the control group (p<0.05). The results of yield traits, back fat thickness, rib eye area, and yield index were similar between the two groups. Carcass weights were 363.4 ㎏ and 353.1 ㎏ for treatment and control, respectively, but these differences were not statistically significant. Quality traits such as meat color, fat color, texture, and maturity were also not significantly different between the two groups. However, marbling score and rate of appearance of high quality grade (1??, 1?, and 1) were higher in the treatment group than in the control group. These results suggest that feeding squid by-products may improve the growth performance and carcass characteristics of Hanwoo cows.

Physicochemical characteristics and fatty acid profiles of muscle tissues from Hanwoo steers fed a total mixed ration supplied with medicinal plant by-products

Lee, Shin Ja,Kim, Do Hyung,Yang, Han Sul,Nam, Ki Chang,Ahn, Seung Kyu,Park, Sung Kwon,Choi, Chang Weon,Lee, Sung Sill Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.10

Objective: Using medicinal plant by-products (MPBP) as feed additives may be an eco-friendly option as substitutes for feedstuffs and may assist in reducing the improper disposal of MPBP. Therefore, this study was conducted to evaluate the influences of MPBP on the meat quality of Hanwoo steers fed a total mixed ration (TMR). Methods: Twenty seven steers (body weight = $573{\pm}57kg$) were randomly divided into three treatments with a control group and two tested groups as follows: control, 1,000 g/kg TMR; treatment 1 (MPBP30), 970 g/kg TMR and 30 g/kg MPBP; treatment 2 (MPBP50), 950 g/kg TMR and 50 g/kg MPBP. Results: Average daily gain, feed conversion ratio and the Commission Internationale de l'Eclairage $L^*$ of muscle were improved (p<0.05, respectively) by MPBP30. Stearic acid ($C_{18:0}$) was decreased (linear effect, p = 0.012), while oleic acid ($C_{18:1}$) was increased (linear effect, p = 0.055) by MPBP level. Saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) were decreased for MPBP50 while unsaturated fatty acid (USFA) and monounsaturated fatty acid (MUFA) were increased for MPBP 50. USFA and SFA ratio was increased for MPBP50 as well. Conclusion: These results indicated that MPBP supplementation in Hanwoo steers fed a TMR increased feed efficiency and meat color (lightness) with altering fatty acid proportions. Therefore, MPBP may be successfully used in ruminant feeding.

Effects of Reed (Phragmites communis) on Growth Performance, Carcass Characteristics, and Meat Quality in Hanwoo Steers

Sang Min Lee(이상민),Sun Sik Chang(장선식),Ki Yong Chung(정기영),Hyeong Cheol Kim(김형철),Sun Ho Choi(최순호),Ha Yeon Jeong(정하연),Boh Suk Yang(양보석),Sung Sill Lee(이성실),Young Moo Cho(조영무) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

본 연구는 한우 거세우의 비육시 급여 조사료의 종류가 발육 및 도체특성에 미치는 영향을 구명하고자 수행하였다. 한우 거세우 21두를 3처리(건초, 갈대 및 갈대+볏짚), 처리당 7두씩 완전 임의 배치하였다. 시험결과 개시체 중 및 종료체중은 처리구별(대조, T1 및 T2)로 각각 125.5, 128.3 및 128.3 kg과 697.4, 614.6 및 706.7 kg으로 나타났다. 일당증체량은 대조구(0.70 kg/d)와 T2 처리구(0.71 kg/d)가 T1 처리구(0.60 kg/d)보다 향상된 경향을 나타났지만 유의적인 차이는 없었다. 건물섭취량은 유의적인 차이는 없었지만 T1 처리구가 다른 처리구에 비해 비교적 낮았다. 육량특성에서 도체중은 대조구와 T2처리구간에는 차이가 없었지만, T2처리구는T1 처리구에 비해 높았다(p<0.05). 등지방두께와 등심단면적은 대조구와 T2처리구가 T1처리구 보다 높았고, 육량등급(A:B:C, %)은 T1 처리구(43:57:0)가 다른 처리구(대조구 0:71:29, T2 29:42:29)에 비해 높았다. 육질특성에서는 지방색과 조직감은 처리구 간 유의적인 차이가 없었다. 그러나 육색과 성숙도는 T2 처리구에 비해 T1 처리구가 유의적으로 높았다 (p<0.05). 근내지방도 및 육질1등급이상 출현율은 T1처리구에 비해 대조구와 T2처리구가 높았다. 본 연구의 결과를 종합해 볼 때 거세한우 비육시 조사료로서 갈대와 볏짚을 혼합급여할 때 발육, 사료 이용성 및 도체 성적이 향상되어 갈대가 한우의 조사료원으로서 활용가치가 있는 것으로 판단되었다. This study investigated the effects of diet of different forages on growth performance and carcass characteristics of Hanwoo steers. Twenty-one Hanwoo steers were randomly allocated to three groups (fed hay, reed, and reed with rice straw) of seven steers each. Initial and final body weights of control, T1, and T2 groups were 125.5, 128.3, 128.3 kg and 697.4, 614.6, 706.7 kg, respectively. Average daily gain tended to increase in controls (0.70 kg/d) and the T2 group (0.71 kg/d) but not as much in the T1 group (0.60 kg/d); however, there was no significant difference. DMI was not significantly different among the treatment groups, but T1 was relatively lower than the other groups. For the yield traits, carcass weight was not significantly different between controls and the T2 group but was greater in the T2 group compared to the T1 group (p<0.05). Back fat thickness and rib eye area were higher in controls and T2 compared to T1; yield grade (A:B:C, %) was greater in T1 (43:57:0) compared to the other groups (control 0:71:29; T2 29:42:29). For the quality traits, fat color and texture were not significantly different among groups. However, meat color and maturity were significantly greater in T1 compared to T2 (p<0.05). Marbling score and appearance rate of over 1st meat quality grade were greater in the control and T2 groups compared to the T1 group. Based on the results, growth performance, feed utilization, and carcass traits appeared to improve when roughage containing rice straw plus reed was offered. Therefore, reed is worth considering as a roughage source for fattening Hanwoo steers.

Effect of optimal sodium stearoyl-2-lactylate supplementation on growth performance and blood and carcass characteristics in Hanwoo steers during the early fattening period

Lee, Shin Ja,Lee, Sang Suk,Kim, Eun Tae,Jeong, Jin Suk,Lee, Ji Hoon,Jeong, Joon,Park, Joong Kook,Park, Beom Young,Jeong, Ha Yeon,Ki, Kwang Seok,Kim, Chang Hyun,Lee, Sung Sill Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9

Objective: This study was conducted to evaluate the effect of different levels of total digestible nutrients (TDN) and sodium stearoyl-2-lactylate (SSL) supplementation on growth performance and blood and carcass characteristics in Hanwoo steers during the early fattening period. Methods: Sixty Hanwoo steers (average body weight, $333{\pm}36.4kg$) were randomly allotted to 3 treatments, with twenty steers per treatment, and ten steers per pen with a size of $80m^2$. Dietary treatments were as follows: CON, basal diet; treatment (TRT) 0.5, 0.5% down-spec of TDN with 0.1% SSL; TRT 1.0, 1.0% down-spec of TDN with 0.1% SSL. Results: The results demonstrated that average daily gain and feed efficiency increased with TRT 0.5 (0.85 kg and 11.68) vs CON (0.82 kg and 11.27) or TRT 1.0 (0.78 kg and 10.74), indicating that 0.1% SSL supplementation in the feed of early fattening steers may result in a saving of 0.5% TDN. No significant differences were observed amongst all treatments (p>0.05) for blood metabolite concentration and blood corpuscle values, which were all within the normally accepted range for healthy steers. Conclusion: Our study suggests that a TDN 0.5% down spec with 0.1% SSL supplemented feed may be effective and profitable for the early fattening period of Hanwoo steers without causing adverse effects.

반추기축영양 : 한우 암소 비육시 배합사료 급여형태가 발육, 혈액성상 및 도체특성에 미치는 영향

이상민 ( Sang Min Lee ),장선식 ( Sun Sik Chang ),김형철 ( Hyeong Cheol Kim ),최순호 ( Sun Ho Kim ),권응기 ( Eung Gi Kwon ),박병기 ( Byung Ki Park ),양보석 ( Boh Suk Yang ),이성실 ( Sung Sill Lee ),조영무 ( Young Moo Cho ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.1

본 연구는 한우 암소 비육시 배합사료 급여형태가 발육, 혈액성상 및 도체특성에 미치는 영향을 구명하고자 수행하였다. 처리구는 배합사료 제한급여 8개월(T1), 제한급여 6개월 후 자유채식 2개월(T2) 및 제한 4개월 후 자유 4개월급여(T3)로 3처리 9반복으로 완전임의배치 하였다. 배합사료 급여형태별 발육은 처리구간 유의적인 차이가 없었다. 그러나, 일당증체량은 T2 처리구(0.75kg/d)가 다른 처리구(T1: 0.62kg/d, T3: 0.72kg/d)에 비해 높은 경향을 나타내었다. 건물섭취량은 처리구간 유의적인 차이는 없었지만 T1 처리구의 볏짚 섭취량이 다른 처리구 보다 유의적으로 높았다(p<0.05). 사료요구량은 T1: 17.8, T2: 12.8 및 T3: 14.1 kg으로 처리구간 유의적인 차이가 인정되었다(p<0.05). 혈청 내 albumin, triglyceride, glucose 및 GPT의 농도는 비육개시 6개월에서 8개월에 T3 처리구가 다른 처리구에 비해 유의적으로 높게 나타났다(p<0.05). 육량특성에서 도체중, 등지방두께 및 등심단면적은 처리구간 유사한 결과를 보였는데 육량지수는 T2 처리구가 T1 처리구에 비해 유의적으로 높게 나타났다(p<0.05). 육량 A등급 출현율은 T2 처리구가 78%로 모든 처리구에서 가장 높았다. 육질특성 중 근내지방도, 육색, 지방색, 조직감 및 성숙도는 처리구간에 유의적인 차이가 나타나지 않았다. 하지만, 근내지방도와 육질 1등급 이상출현율은 T3 처리구(4.0, 56%)가 다른 처리구(T1: 3.4, 56%; T2: 3.6, 33%)에 비해 높은 경향을 나타내었다. 따라서 본 연구는 한우 암소 비육시 배합사료의 급여는 비육초기 4개월은 제한급여(체중의 1.6%) 하고 비육후기 4개월에 자유채식하는 것이 적절한 것으로 판단되었다. This study was conducted to investigate the effects of feeding patterns of concentrates on growth performance, blood parameters and carcass characteristics in Hanwoo cows. Randomly-allocated groups, restricted concentrate feeding(T1), restricted concentrate feeding for 6 months and ad libitum 2 months(T2), restricted concentrate feeding for 4 months and ad libitum 4 months(T3), were contained with 9 animals. According to feeding patterns of concentrate, growth performance was not significantly different among the treatment groups. However, ADG tended to be higher in T2 group(0.75kg/d) compared to the other groups (T1: 0.62 kg/d, T3: 0.72kg/d). DMI was not significantly different among the treatment groups, interestingly, rice straw intake was significantly higher in T1 group compared with others(p<0.05). There were significant difference among feed conversion ratio, which are 17.8, 12.8, and 14.1 kg for T1, T2, and T3(p<0.05), respectively. The serum level of albumin, triglyceride, glucose and GPT were greater in T3 group compared to other groups at fattening 6 to 8 months(p<0.05). The results of yield traits, carcass weight, back fat thickness and rib eye area were not differ among treatment groups, but yield index was significantly greater in T2 group compared to T1 group(p<0.05). The ``A`` appearance rate(%) of meat yield grade was highest in T2 group for 78%. The marbling score, meat color, fat color, texture and maturity in quality traits were not differ among the treatment groups. However, marbling score and appearance rate(%) of over 1st meat quality grade were tend to be increased at T3 rather (4.0, 56%) group compared with other groups(T1: 3.4, 56%; T2: 3.6, 33%). Thus, the present study suggested that restricted concentrate feeding(1.6% of BW) for 4 months during early fattening periods and ad libitum feeding for 4 months during late fattening periods are recommendable.

Blood and milk metabolites of Holstein dairy cattle for the development of objective indicators of a subacute ruminal acidosis

Hyun Sang Kim,Jun Sik Eom,Shin Ja Lee,Youyoung Choi,Seong Uk Jo,Sang Suk Lee,Eun Tae Kim,Sung Sill Lee Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.8

Objective: The purpose of this study was to perform a comparative analysis of metabolite levels in serum and milk obtained from cows fed on different concentrate to forage feed ratios. Methods: Eight lactating Holstein cows were divided into two groups: a high forage ratio diet (HF; 80% Italian ryegrass and 20% concentrate of daily intake of dry matter) group and a high concentrate diet (HC; 20% Italian ryegrass and 80% concentrate) group. Blood was collected from the jugular vein, and milk was sampled using a milking machine. Metabolite levels in serum and milk were estimated using proton nuclear magnetic resonance and subjected to qualitative and quantitative analyses performed using Chenomx 8.4. For statistical analysis, Student's t-test and multivariate analysis were performed using Metaboanalyst 4.0. Results: In the principal component analysis, a clear distinction between the two groups regarding milk metabolites while serum metabolites were shown in similar. In serum, 95 metabolites were identified, and 13 metabolites (include leucine, lactulose, glucose, betaine, etc.) showed significant differences between the two groups. In milk, 122 metabolites were identified, and 20 metabolites (include urea, carnitine, acetate, butyrate, arabinitol, etc.) showed significant differences. Conclusion: Our results show that different concentrate to forage feed ratios impact the metabolite levels in the serum and milk of lactating Holstein cows. A higher number of metabolites in milk, including those associated with milk fat synthesis and the presence of Escherichia coli in the rumen, differed between the two groups compared to that in the serum. The results of this study provide a useful insight into the metabolites associated with different concentrate to forge feed ratios in cows and may aid in the search for potential biomarkers for subacute ruminal acidosis.

무기물성 및 식물성 생리활성 물질이 반추위 미생물의 성장에 미치는 영향

신성환(Sung Whan Shin),이신자(Shin Ja Lee),옥지운(Ji Un Ok),이상민(Sang Min Lee),임정화(Jung Hwa Lim),김경훈(Kyoung Hoon Kim),문여황(Yea Hwang Moon),이성실(Sung Sill Lee) 한국생명과학회 2007 생명과학회지 Vol.17 No.11

본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli 에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. In vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다. In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.