2D FFT를 이용한 움직임 검출에 관한 연구

김혜경,남시병 三陟大學校 2002 論文集 Vol.35 No.-

In this paper, we proposed the algorithm that the motion block detection in the difference image using the 2D FFT This algorithm can be minimize the effects of noise, after detecting the motion blocks from difference spectrum using the 2D FFT in the input images that changes of brightness occurs. Firstly, we get the difference spectrum that be detected after subtracting the background and input image frequency spectrum. and then, it passes the high pass filter for removing the low frequency domain that included the same spectrum. And reconstructs difference image to time domain using the 2D IFFT again. Then, it can be detected the motion block comparing the unit pixel blocks with block mean value sequentially. Also, the error rate of motion block detections appeared similarly all about 11% regardless of the brightness change of input images. It has been confirmed that the adaptation to noise is robust.

UV-TiO₂광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정해광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

UV-TiO_(2) 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254 ㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_(2)는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7× 10^(7) cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0× 10^(2) cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 3.4× 10 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 석영관에 박막증착된 경우에는 7.6× 10^(2) cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입후 11분 동안 자외선을 조사시킨 경우에는 1.3× 10^(2) cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 1× 100 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 박막증착된 경우에는 7.9× 10 cells/㎖을 나타내었다. 결국 UV-TiO_(2) 반응기에 사용되는 TiO_(2)가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_(2) photocatalytic system on the Escherichia coli DH5-慣 and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 nm was set on the center of pyrex round glass tube. Two types of TiO_(2), one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7 * 10^(5) cells/??of E. coli for 11 min, the living cell number decreased to 4.0 * 1.0^(0) cells/?? The effect of UV system together with slurried TiO_(2) was less than 1 cells/?? whereas that of UV-coated TiO_(2) system decreased to 7.1 * 10^(3) cells/?? To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7 * 10^(5) cells/??were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3 x 10^(2) cells/?? In the same condition except the addition of slurried TiO_(2), the living cells were 1 * 10^(2) cells/?? However, more cells could be killed by the system which consists of UV, coated TiO_(2), and air bubble (7.9 * 10^(1) cells/??. From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_(2) system.

중합효소연쇄반응 기법을 이용한 돼지 증식성 장염 진단 기법의 확립

김대영,권언혜,송민동,양시용 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1

This study was carried out to establish a efficient diagnosis method of pocine proliferative enteropathy (PPE) contributing major economic loss by inducing weight loss, poor growth and/or sudden death through out the growth period(6~22 wk). Therefore, in this study we established the PCR (polymerase chain reaction) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L. intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L. intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect lng of genomic DNA as a template. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific dignistic tool for PPE.

UV-TiO_2 광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정혜광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경연구소 1998 環境硏究 Vol.14 No.1

UV-TiO_2 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_2는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7×10^7 cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0×10^2 cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 3.4×10 cells/㎖으로, 자외선 조사와 함께 TiO_2가 석영관에 박막증착된 경우에는 7.6×10^2 cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입수 11분 동안 자외선을 조사시킨 경우에는 1.3×10^2 cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 1×102 cells/㎖으로, 자외선 조사와 함께 TiO_2가 박막증착된 경우에는 7.9×10 cells/㎖을 나타내었다. 결국 UV-TiO_2 반응기에 사용되는 TiO_2가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_2 photocatalytic system on the Escherichia coli DH5-α and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 ㎚ was set on the center of pyrex round glass tube. Two types of TiO_2, one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7×10^5 cells/㎖ of E. coli for 11 min, the living cell number decreased to 4.0×10^0 cells/㎖. The effect of UV system together with slurried TiO_2 was less than 1 cells/㎖, whereas that of UV-coated TiO_2 system decreased to 7.1×10^3 cells/㎖. To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7×10^5 cells/㎖ were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3×10^2 cells/㎖. In the same condition except the addition of slurried TiO_2, the living cells were 1×10^2 cells/㎖. However, more cells could be killed by the system which consists of UV, coated TiO_2, and air bubble (7.9×10^1 cells/㎖). From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_2 system.

토양 미생물인 Streptomyces tubercidicus에서 분리한 GTPcyclohydrolase Ⅰ 저해제

한지만,김시욱,이인화,박열,정혜광,윤성명,유진철 조선대학교 약학연구소 1998 藥學硏究誌 Vol.19 No.1

GTP cyclohydrolase I (EC 3.5.4.16) catalyzes the conversion of GTP to D-erythro-7,8-dihydroneopterin triphosphates and formic acid. and thus performs the first committing step in the biosynthesis of the pteridine moiety of folk acid in microorganisms and of tetrahydrobiopterin in higher animals. GTP cyclohydrolase I isolated from Streptomyces tubercidicus was inhibited by DL-6-methyl-5,6,7,8-tetrahydropterin and xanthopterin, and was not inhibited by tetrahydrofolic acid, pterin, pterin-6-carboxylic acid, biopterin, neopterin, ribofravin. These results suggest that bacterial GTP cyclohydrolase I may be regulated by its metaboilic end product.

개에서 관찰된 모기질세포종의 증례보고

조성환,손화영,김혜성,최종윤,류시윤,박배근,윤원기,홍성혁,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-

Pilomatricoma was histopathologically diagnosed in a 2.5-year-old male Alaskan malamute. The patient was referred to Chungnam National University, Veterinary Medical Teaching Hospital with solitary, firm and well-circumscribed mass at shoulder. Grossly, the mass was dome shaped to tumor like and 10 x 8 x 5㎝ in size. Some lesions had gritty or bony consistency and was chalky when sectioned Histopathologic feature included mumerous clusters of tightly arranged, basaloid cells with evenly distributed chromatin and ghost cells were characterized by a central unstained zone. The ghost cells often become calcified and osseous metaplasia was also present.

농장 사육되는 타조에서 나타난 다리 기형의 증례 보고

최종윤,손화영,전무형,조성환,김혜성,류시윤,박배근,이영원,윤원기 충남대학교 수의과대학 동물의과학연구소 2001 動物醫科學硏究誌 Vol.9 No.-

Two farmed young ostrich chicks were applied to pathology laboratory at Chungnam National University. Grossly, one ostrich showed rotated tibiotarsus, the other showed rolled toes. Case 1 ostrich was hypertrophy of fibualis longus and tibial cranialis tendon and gastrocnemius tendon in crus, inflammation of flexor digitorum longus. In histopathological views, myocytes of fiburalis longus were showed hypertrophy and hyperplasia. It is not found any specific feature in X-ray of the case 2 ostrich. Limb deformities were detected frequently in farmed ostrich chicks in 2- to 3 weeks old. Limb deformities were an important cause of mortality in farmed ostrich chicks during the first 10weeks after hatch. Cause of limb deformities were considered a nutrition problem.

[PB-0046] Overexpression of IbMYB1a gene induced anthocyanin accumulation in soybean seeds

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Hye Jeong Kim(Hye Jeong Kim),Hae Gyun Park(Hae Gyun Park),Hye Min Park(Hye Min Park),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0047] Inhibition of gene expression for anti-nutritional factors and allergen in soybean seeds via RNAi-mediated co-transformation

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Da Hyeon Ha(Da Hyeon Ha),Hye Jeong Kim(Hye Jeong Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Risk Factors for Coronavirus Disease 2019 (COVID-19)-Associated Pulmonary Aspergillosis in Critically Ill Patients: A Nationwide, Multicenter, Retrospective Cohort Study

Kim Si-Ho,Hong Jin Yeong,Bae Seongman,Lee Hojin,Wi Yu Mi,Ko Jae-Hoon,Kim Bomi,Joo Eun-Jeong,Seok Hyeri,Shi Hye Jin,Yoo Jeong Rae,Hyun Miri,Kim Hyun ah,Jang Sukbin,Mun Seok Jun,Kim Jungok,Kim Min-Chul 대한의학회 2022 Journal of Korean medical science Vol.37 No.18

Background: Coronavirus disease 2019 (COVID-19) is often accompanied by secondary infections, such as invasive aspergillosis. In this study, risk factors for developing COVID-19- associated pulmonary aspergillosis (CAPA) and their clinical outcomes were evaluated. Methods: This multicenter retrospective cohort study included critically ill COVID-19 patients from July 2020 through March 2021. Critically ill patients were defined as patients requiring high-flow respiratory support or mechanical ventilation. CAPA was defined based on the 2020 European Confederation of Medical Mycology and the International Society for Human and Animal Mycology consensus criteria. Factors associated with CAPA were analyzed, and their clinical outcomes were adjusted by a propensity score-matched model. Results: Among 187 eligible patients, 17 (9.1%) developed CAPA, which is equal to 33.10 per 10,000 patient-days. Sixteen patients received voriconazole-based antifungal treatment. In addition, 82.4% and 53.5% of patients with CAPA and without CAPA, respectively, received early high-dose corticosteroids (P = 0.022). In multivariable analysis, initial 10-day cumulative steroid dose > 60 mg of dexamethasone or dexamethasone equivalent dose) (adjusted odds ratio [OR], 3.77; 95% confidence interval [CI], 1.03–13.79) and chronic pulmonary disease (adjusted OR, 4.20; 95% CI, 1.26–14.02) were independently associated with CAPA. Tendencies of higher 90- day overall mortality (54.3% vs. 35.2%, P = 0.346) and lower respiratory support-free rate were observed in patients with CAPA (76.3% vs. 54.9%, P = 0.089). Conclusion: Our study showed that the dose of corticosteroid use might be a risk factor for CAPA development and the possibility of CAPA contributing to adverse outcomes in critically ill COVID-19 patients.