An Investigation on Inorganic Arsenic in Seaweed by Ion Chromatography Combined with Inductively Coupled Plasma-Atomic Emission Spectrometry

Sheng Cui,Jin-Su Na,Na-Young Kim,이용훈,남상호 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

This study focused on the matrix interference for the qualitative and quantitative analysis of inorganic arsenic species in seaweed by ion chromatography (IC) combined with inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The matrix of seaweed has a significant effect on the determination of inorganic arsenic species. In particular, the retention times of inorganic arsenic species in the in the standard solution were different from those in seaweed because of the matrix interference. Thus, it was not suitable to use the chromatographic method for the determination of the arsenic species in seaweed. We investigated an alternative method for the determination of inorganic arsenic species in seaweed. The method was applied for the seaweed samples such as laver, green laver, sea tangle and sea mustard. The sample extraction methods of the arsenic species were also investigated in this study.

Study on the analytical method of arsenic species in marine samples by ion chromatography coupled with mass spectrometry

Cui, Sheng,Kim, Chan Kook,Lee, Kyoung-Seok,Min, Hyung Sik,Lee, Joung Hae Elsevier 2018 Microchemical journal Vol.143 No.-

<P><B>Abstract</B></P> <P>The present study focused on the analytical method of the arsenic species in various marine samples (seafood and marine sediment). Five inorganic and organic arsenic species (arsenite, arsenate, monomethylarsonic, dimethylarsinic and arsenobetaine) were investigated. The extraction method for seafood was chosen with sonication in which a water:methanol mixture (1:1, v/v) was used as the extract solvent. The arsenic species in marine sediment were extracted by shaking using 0.5 M phosphoric acid as extract solvent. The species were separated by a Hamilton-X100 analytical column, and detected by a mass spectrometry separately. Due to the interference caused by ArCl at mass 75, we tried to detect the arsenic at mass 91 by combining arsenic with oxygen (AsO), and the interference of ArCl was eliminated successfully. The seafood certified reference material was used to validate the method for the extraction of arsenic species in seafood samples, and the recovery efficiency of arsenobetaine was detected as 89.4% by this method. For marine sediment, inorganic standard solutions were spiked to confirm the recovery efficiency. The results showed that the recovery efficiencies were 85.7% and 82.2% for arsenite and arsenate. The analysis method was also applied to many real marine samples. The extraction efficiencies of arsenic species were 79.2%–90.6% and 57.4%–91.3% for the real seafood and marine sediment samples.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Toxic and biological effects of arsenic depend on its special chemical forms. </LI> <LI> Method for analysis of arsenic species in marine samples by IC-ICP-MS was investigated. </LI> <LI> Interference caused by ArCl at mass 75 was eliminated successfully. </LI> <LI> This method was also applied to various CRMs and real marine samples. </LI> </UL> </P>

Water-soluble coenzyme Q₁₀ provides better protection than lipid-soluble coenzyme Q₁₀ in a rat model of chronic tacrolimus nephropathy

( Sheng Cui ),( Kang Luo ),( Yi Quan ),( Sun Woo Lim ),( Yoo Jin Shin ),( Kyung Eun Lee ),( Hong Lim Kim ),( Eun Jeong Ko ),( Ju Hwan Kim ),( Sang J. Chung ),( Soo Kyung Bae ),( Byung Ha Chung ),( Chu 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.4

Background/Aims: Coenzyme Q₁₀ (CoQ₁₀), is a promising antioxidant; however, low bioavailability owing to lipid-solubility is a limiting factor. We developed water-soluble CoQ₁₀ (CoQ₁₀-W) and compared its effects with conventional lipid-soluble CoQ₁₀ (CoQ₁₀-L) in an experimental model of chronic tacrolimus (Tac) nephropathy. Methods: CoQ₁₀-W was developed from a glycyrrhizic-carnitine mixed layer CoQ₁₀ micelle based on acyltransferases. Chronic nephropathy was induced in rats with 28-day Tac treatment; they were concomitantly treated with CoQ₁₀-L or CoQ₁₀-W. CoQ₁₀ level in plasma and kidney were measured using liquid chromatography-mass spectrometry. CoQ₁₀-W and CoQ₁₀-L effects on Tac-induced nephropathy were assessed in terms of renal function, histopathology, oxidative stress, and apoptotic cell death. Their effects on cell viability and reactive oxygen species (ROS) production were assessed in cultured proximal tubular cells, human kidney 2 (HK-2) cells. Results: The plasma CoQ₁₀ level was significantly higher in the CoQ₁₀-W group than in the CoQ₁₀-L group. Tac treatment caused renal dysfunction, typical pathologic lesions, and oxidative stress markers. Serum creatinine was restored in the Tac + CoQ₁₀-L or CoQ₁₀-W groups compared with that in the Tac group. CoQ₁₀-W administration reduced oxidative stress and apoptosis markers. Mitochondrial ultrastructure assessment revealed that the addition of CoQ₁₀-L or CoQ₁₀-W with Tac increased mitochondrial size and number than Tac treatment alone. In vitro investigations revealed that both CoQ₁₀-L and CoQ₁₀-W improved cell viability and reduced ROS production in the Tac-induced HK-2 cell injury. Conclusions: CoQ₁₀-W has a better therapeutic effect in Tac-induced renal injury than conventional CoQ₁₀-L, possibly associated with improved CoQ₁₀ bioavailability.

An Investigation on Inorganic Arsenic in Seaweed by Ion Chromatography Combined with Inductively Coupled Plasma-Atomic Emission Spectrometry

Cui, Sheng,Na, Jin-Su,Kim, Na-Young,Lee, Yonghoon,Nam, Sang-Ho Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

This study focused on the matrix interference for the qualitative and quantitative analysis of inorganic arsenic species in seaweed by ion chromatography (IC) combined with inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The matrix of seaweed has a significant effect on the determination of inorganic arsenic species. In particular, the retention times of inorganic arsenic species in the in the standard solution were different from those in seaweed because of the matrix interference. Thus, it was not suitable to use the chromatographic method for the determination of the arsenic species in seaweed. We investigated an alternative method for the determination of inorganic arsenic species in seaweed. The method was applied for the seaweed samples such as laver, green laver, sea tangle and sea mustard. The sample extraction methods of the arsenic species were also investigated in this study.

A Study of in vitro Scavenging Reactions of Acrylamide with Glutathione Using Electrospray Ionization Tandem Mass Spectrometry

Cui, Sheng-Yun,Kim, Seung-Jin,Jo, Sung-Chan,Lee, Yong-Moon,Lee, Yong-Ill Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.8

A combination of electrospray ionization and tandem mass spectrometry was used to characterize the scavenging reactions of acrylamide (AA) in the presence of glutathione (GSH) in vitro. In the presence of GSH, AA was deactivated effectively and scavenged by reactions consuming small amount of GSH. Reaction products and structural information were identified using collision-induced dissociation (CID) in an ion trap mass spectrometer. In the mixture of GSH and AA, significant increase in abundance of fragment ion peak was observed at m/z 233, which was identified as $[Cys-Glu]^+$, formed by the elimination of glycine moiety of GSH. GSH also contributes to the AA scavenging reaction by conjugating with AA through the sulfhydryl group in cysteine moiety. The probable scavenging reaction pathway of AA in the presence of GSH has been proposed based on the CID experimental data.

Differentiating Benign from Malignant Bone Tumors Using Fluid-Fluid Level Features on Magnetic Resonance Imaging

Hong Yu,Jian-Ling Cui,Sheng-Jie Cui,Ying-Cai Sun,Feng-Zhen Cui 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.6

Objective: To analyze different fluid-fluid level features between benign and malignant bone tumors on magnetic resonance imaging (MRI). Materials and Methods: This study was approved by the hospital ethics committee. We retrospectively analyzed 47 patients diagnosed with benign (n = 29) or malignant (n = 18) bone tumors demonstrated by biopsy/surgical resection and who showed the intratumoral fluid-fluid level on pre-surgical MRI. The maximum length of the largest fluid-fluid level and the ratio of the maximum length of the largest fluid-fluid level to the maximum length of a bone tumor in the sagittal plane were investigated for use in distinguishing benign from malignant tumors using the Mann-Whitney U-test and a receiver operating characteristic (ROC) analysis. Fluid-fluid level was categorized by quantity (multiple vs. single fluid-fluid level) and by T1-weighted image signal pattern (high/low, low/high, and undifferentiated), and the findings were compared between the benign and malignant groups using the χ2 test. Results: The ratio of the maximum length of the largest fluid-fluid level to the maximum length of bone tumors in the sagittal plane that allowed statistically significant differentiation between benign and malignant bone tumors had an area under the ROC curve of 0.758 (95% confidence interval, 0.616–0.899). A cutoff value of 41.5% (higher value suggests a benign tumor) had sensitivity of 73% and specificity of 83%. Conclusion: The ratio of the maximum length of the largest fluid-fluid level to the maximum length of a bone tumor in the sagittal plane may be useful to differentiate benign from malignant bone tumors.

Expression and Clinical Significance of miRNA-34a in Colorectal Cancer

Ma, Zhi-Bin,Kong, Xiao-Lin,Cui, Gang,Ren, Cui-Cui,Zhang, Ying-Jie,Fan, Sheng-Jin,Li, Ying-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Materials and Methods: Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA-34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. Results: After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). Conclusions: miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.

Cloning and Expression of cDNA Encoding a Cysteine Protease Inhibitor from Clamworm and Its Possible Use in Managing Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae)

( Sheng Nan Li ),( Dao Sen Guo ),( Bo Guang Zhao ),( Jian Ling Ye ),( Jie Tian ),( Wen Qing Ren ),( Yun Wei Ju ),( Peng Cui ),( Rong Gui Li ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8

A cDNA encoding a cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc: 153129 from Danio rerio, cystatin B from Theromyzon tessulatum, and the ChainA, stefin B tetramer from Homo sapiens, respectively. The gene was cloned into the intracellular expression vector pET-15b and expressed in Escherichia coli. The recombinant CPI (PA-CPI) was purified by affinity chromatography on Nicharged resin and ion-exchange chromatography on DEAE-Sepharose FF. The relative molecular mass of PACPI was 16 kDa as deduced by SDS-PAGE. Activity analysis showed that the recombinant protein could inhibit the proteolytic activity of papain. A constitutive and secretive expression vector was also constructed, and the cDNA encoding CPI was subcloned into the vector for extracellular expression. Western blotting analysis results showed that the PA-CPI was secreted into the medium. Bioassay demonstrated that E. coli DH5α harboring pUC18ompAcat-CPI showed a significant difference in mortality to the Asian longhorned beetle Anoplophora glabripennis compared with untransformed E. coli DH5α and control.

The Relative Influence of Diet and Physical Activity on Obesity in China

Cui Zhao-Hui,Li Yan-Ping,Di Yu-Feng,Ba Lei,Hu Xiaoqi,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to investigate the relative influence of diet and physical activity on obesity. The subjects were 155 adults aged 35-52 years from 24 neighborhood committees in 4 urban districts of Beijing (male : 78, female : 77). They were divided into normal weight, overweight and obese groups according to their BMI. The general information of the subjects was collected by interview-administered questionnaire. Dietary intake was obtained by three-day(two weekdays and one weekend day) food weighted method, physical activity was assessed by a validated combination of data obtained from activity monitors, bicycling information and activity records. There were no significant differences of age, gender, height, educational, family economic level, smoking and drinking between different groups. The proportion of flour intake was higher in obese group compared to normal weight and overweight groups, and that of vegetables is lower in obese group. The physical activity (PAL) was not significantly different between two groups of the normal, overweight and obese groups. After the adjustment for confounding factors using logistic regression model, we found that the proportion of flour intake was positively associated with obesity, while the proportion of vegetable intake was inversely associated with obesity. It is concluded that dietary patterns were associated with obesity and diets composed of more vegetables and less staple combined with physical activities could contribute to obesity prevention.

Relative Risk of Metabolic Syndrome in Middle Aged Adults with Different Weight Living in Urban Beijing, China

Cui Zhao-Hui,Li Yan-Ping,Liu Ai-Ling,Zhang Qian,Du Wei-Jing,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to compare the relative risk of metabolic syndrome (MS) in middle aged adults with different body weights. 155 subjects living in urban Beijing were recruited from 24 neighborhood committees of urban Beijing. They were divided into normal weight, overweight and obese groups according to their BMIs. The general information of the subjects was collected using an interview-administered questionnaire. Standard procedure was followed to measure subject's weight, height and waist. Biochemical parameters (total cholesterol (TC), low- and high­density lipoprotein cholesterol (LDL-C ; HDL-C), triglyceride (TG), and fasting glucose) and blood pressure were also determined. The results indicated that the systolic and diastolic blood pressure, HDL-C of obese group was lower than that of the normal weight group. Fasting glucose of obese males was significantly higher than that of normal weight males. No significant difference of fasting glucose was found among female groups. No significant difference of TG was found among male groups, while TG of overweight and obese females was both significantly higher than normal weight females. There was no significant difference of TC and LDL-C among normal weight, overweight and obese groups in both males and females. The MS rate of obese males was significantly higher than the normal weight and overweight males, as was the female. The relative risk of MS in obese group was about 11 times higher (OR=11.249, $95\%CI$ = 3.812 - 33.191) than the normal weight group after adjusting for age, gender, smoking, drinking, family economic level and education status. It is concluded that obesity contributed to lower HDL-C, hypertriglyceride, hypertension and MS after controlling the effects of age, gender, socioeconomic status, alcohol drinking and smoking. Obese individuals have a higher risk of having MS than their normal weight counterparts.