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( Seonghee Ahn ),( Yoon-hyung Yim ),( Byungjoo Kim ) 한국질량분석학회 2013 Mass spectrometry letters Vol.4 No.4
A gas chromatography/ mass spectrometric (GC/MS) method was developed as a candidate reference method for the accurate determination of essential fatty acids (linoleic acid, α- and γ-linolenic acids) in food supplemental oil products. Samples were spiked with three internal standards (stearic acid-d35, 13C18-linoleic acid, and 13C18-α-linolenic acid). Samples were then subject to saponification, derivatization for methylation, and extraction by organic solvent. For GC/MS measurement, an Agilent HP-88 column, designed for the separation of fatty acid methyl esters, was selected after comparing with other columns as it provided better separation for target analytes. Target analytes and internal standards were detected by selected ion monitoring of molecular ions of their methyl ester forms. The GC/MS method was applied for the measurement of three botanical oils in NIST SRM 3274 (borage oil, evening primrose oil, and flax oil), and measurement results agreed with the certified values. Measurement results for target analytes which have corresponding isotope-labeled analogues as internal standard were calculated based on isotope dilution mass spectrometry (IDMS) approach, and compared with results calculated by using the other two internal standards. Results from the IDMS approach and the typical internal standard approach were in good agreement within their measurement uncertainties. It proves that the developed GC/MS method can provide similar metrological quality with IDMS methods for the measurement of fatty acids in natural oil samples if a proper fatty acid is used as an internal standard.
Ahn, Seonghee,Yim, Yoon-Hyung,Kim, Byungjoo Korean Society for Mass Spectrometry 2013 Mass spectrometry letters Vol.4 No.4
A gas chromatography/mass spectrometric (GC/MS) method was developed as a candidate reference method for the accurate determination of essential fatty acids (linoleic acid, ${\alpha}$- and ${\gamma}$-linolenic acids) in food supplemental oil products. Samples were spiked with three internal standards (stearic acid-$d_{35}$, $^{13}C_{18}$-linoleic acid, and $^{13}C_{18}$-${\alpha}$-linolenic acid). Samples were then subject to saponification, derivatization for methylation, and extraction by organic solvent. For GC/MS measurement, an Agilent HP-88 column, designed for the separation of fatty acid methyl esters, was selected after comparing with other columns as it provided better separation for target analytes. Target analytes and internal standards were detected by selected ion monitoring of molecular ions of their methyl ester forms. The GC/MS method was applied for the measurement of three botanical oils in NIST SRM 3274 (borage oil, evening primrose oil, and flax oil), and measurement results agreed with the certified values. Measurement results for target analytes which have corresponding isotope-labeled analogues as internal standard were calculated based on isotope dilution mass spectrometry (IDMS) approach, and compared with results calculated by using the other two internal standards. Results from the IDMS approach and the typical internal standard approach were in good agreement within their measurement uncertainties. It proves that the developed GC/MS method can provide similar metrological quality with IDMS methods for the measurement of fatty acids in natural oil samples if a proper fatty acid is used as an internal standard.
Seonghee Ahn,김병주,Yunjung Lee,김정권 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.11
Malachite green (MG) has been used world-widely in aquaculture as a parasiticide or fungicide. Although MG performed successfully, it has not been permitted for use in aquaculture from European Union, USA, and Canada because of its carcinogenicity and mutagenicity. We developed a sensitive and specific method to determine MG and its principal metabolite, leucomalachite green (LMG), respectively by isotope dilution liquid chromatography mass spectrometry (ID-LC/MS). To enhance the extraction recovery of MG and LMG from fish tissue, an additional step, saponification,was introduced in sample preparation process to remove fat in sample extract, which hampered the performance of SPE columns. The residue of MG and LMG in fish was analyzed using liquid chromatography mass spectrometry in the selected ion monitoring (SIM) mode by monitoring at m/z 329 and 334 for MG and d5-MG and at m/z 331 and 337for LMG and 13C6-LMG, respectively. This method was validated by comparing with the value of the reference material provided by Laboratory Government Chemistry (LGC). The results agreed within the measurement uncertainty and the accuracy was much improved than the provided reference value by LGC.
일과성 전기억상실증에서의 발작 중 신경심리소견 : 증례보고 A Case Report
권재철,안선영,김성희,진주희,나덕렬 대한치매학회 2004 Dementia and Neurocognitive Disorders Vol.3 No.2
Cases of transient global amnesia (TGA) in which neuropsychological tests were performed during attack are rarely reported. We report a 62-year-old man with TGA who was evaluated with a series of neuropsychological tests during and after attack. During ictus, he showed severe anterograde amnesia for both verbal and visual materials which was accompanied by mild impairment at confrontation naming and counterclockwise rotation of Rey-Osterrieth Complex Figure on delayed recall. At follow-up neuropsychological evalauation 4 days after ictus, he showed complete recovery of the cognitive abnormalities including amnesia.