한우 myostatin 유전자의 SNP 및 발현분석

유성란,정기철,상병찬,이준헌 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

Myostatin is a transforming growth and differentiation factor-β family member that acts as a negative regulator of muscle growth. Previously, mutations in the myostatin gene were known to be related to double muscling phenotypes in cattle. Because myostatin gene is highly related to muscle mass, also meat quality, in cattle, we sequenced whole myostatin mRNA and investigated the SNPs (Single Nucleotide Polymorphisms) in Korean cattle (Hanwoo). The results indicated that Hanwoo had an SNP in nt2385 and this mutation can be a useful marker with further verifications. We also investigated expression patterns of the myostatin gene from various muscle tissues and organs. Northern blotting results indicated that myostatin expression was restricted in muscles with variable expression levels. The results presented here can be used as a valuable information for meat quality related traits and muscle mass in cattle.

알코올에 의해 유발된 진정 및 수행 장애에 대한 카페인의 반전 효과

유남재,김정란,조정혜,왕성근 대한생물치료정신의학회 2004 생물치료정신의학 Vol.10 No.1

연구목적 : 저자들은 알코올의 진정 작용과 수행 붕괴 효과에 대한 카페인의 영향을 확인하기 위해 이 연구를 시행하였다. 방 법 : 건강한 19세에서 25세 사이의 대학생 지원자 14명을 대상으로 Epsworth Slippiness Scale(ESS), Stand-ford Sleepiness Scale(SSS), Visual Analogue Sclae(VAS), Vienna test, Multiple Sleep Latency Test(MSLT) and Breath Ethanol Concentration(BrEC)을 측정하였다. 결 과 : 알코올(1.0g/㎏)은 투여 후 5시간까지 입면잠복기를 단축시키며, 카페인(300mg)은 이러한 단축을 현저히 개선시키는 반전 효과가 있었다. 알코올(1.0g/㎏)은 투여 후 3~5시간 동안 주간 졸음과 피로감을 유발하며, 카페인(150~300mg)은 졸음과 피로감을 개선시키는 효과가 있었다. 경계력은 알코올(0.5~1.0g/㎏) 투여 1시간 후 유의하게 저하되었고 카페인(150~300mg) 투여 3시간 후부터 개선되었으며, 집중력은 알코올(1.0g/㎏) 투여 후 변화가 없었으나 카페인 투여 3시간 후부터 유의하게 향상되었고, 단기 기억은 알코올과 카페인 투여에 유의한 변화가 나타나지 않았다. 기준일, 알코올 투여일, 알코올 및 카페인 투여일에서 각 변량(SSS, MSLT, 졸음, 피로감, 기분, 집중력, 경계력, 단기 기억력)간에는 일관성 있는 유의한 관계가 나타나지 않았다. 정상인에서 알코올은 입면잠복기를 단축시키고 주간 졸음과 피로감을 유발하며, 카페인은 알코올에 의해 유발된 입면잠복기의 단축을 연장시키고 주간 졸음과 피로감 및 경계력과 주의 집중력을 개선시키는 효과가 있었다. 결 론 : 카페인은 알코올의 진정 및 수행 붕괴 효과를 반전시키는 효과가 있음을 알 수 있었다. Objectives : The authors performed this study to identify the effects of caffeine on the alcohoi's sedative and performance disruptive effects. Methods : Epsworth Sleeppiness Scale(ESS), Stanford Sleepiness Scale(SSS), Visual Analogue Scale WAS), Vienna test, Multiple Sleep Latency Test(MSLT) and Breath Ethanol Concentration(BrEc) were checked for the 74 young and healthy college student volunteers aging from 19 to 25 years. Results : Alcohol(1.0g/kg) freduced sleep latency until 5 hours after administration and caffeine(300mg) reversed this effect significantly. Alcohol(1.0g/kg) induced daytime sieepiness and fatigue 3-5 hours after administration and caffeine(l50-300mg) improved sleepiness and fatigue. Vigilance was decreased significantly 1 hour after alcohol administration(0.5-1.0g/kg) and improved 3 hours after caffeine administration(l50-300mg) and attention was not changed after alcohol administration(1.0g/kg) but improved significantly 3 hours after caffeine administration and short-term memory was not changed significantly after alcohol and caffeine administration. On the baseline, the day of alcohol administration only, and alcohol and caffeine administration day, between each vanable(SSS, MSLT, sleepiness, fatigue, mood, vigilance. concentration, and short-term memory) did not show significant consistent correlation. Conclusion : Alcohol decreases sleep latency and induces daytime sleepiness and fatigue, and caffeine prolonges sleep latency and improves daytime sleepiness, fatigue, vigilance and attention in normal subjects. These results showed caffeine reverses alcohol's sedative and performance-disruptive effects.

한우 Band 3 및 CHS 유전병의 분자유전학적 기초연구

정행진,유성란,상병찬,이준헌 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

Many bovine genetic diseases are currently unidentified in Korea because of the relatively low monitoring systems in the livestock farms. The molecular detection system using PCR-RFLP of two genetic diseases, namely Band 3 (Erythrocyte Membrane Protein Band Ⅲ) and CHS (Chediak-Higashi Syndrome), have been identified in Japan and used for screening large number of cattle whether each individual has the genetic disease or not. Using the 22 unrelated Korean cattle (Hanwoo) individuals, molecular detection system based on PCR-RFLP have been investigated, which can be distinguishable carriers for the genetic diseases. Even though we could not found the causative mutations for two genetic diseases, the PCR-RFLP techniques used in this study are very valuable for the screening the genetics diseases in Korean cattle, especially for the proven or candidate bulls.

Histone deacetylase 4 mediates SMAD family member 4 deacetylation and induces 5-fluorouracil resistance in breast cancer cells

YU, SEONG-LAN,LEE, DONG CHUL,SON, JI WOONG,PARK, CHANG GYO,LEE, HOI YOUNG,KANG, JAEKU Spandidos Publications 2013 Oncology reports Vol.30 No.3

<P>Histone deacetylases (HDACs) have been shown to play important roles in the regulation of chromatin remodeling by histone deacetylation, and their expression is induced in several types of cancer. In addition, they are known to be associated with resistance to anticancer drugs. However, the relevance of HDAC4 in chemoresistance remains unclear. Therefore, we investigated the interaction between HDAC4 expression and chemoresistance in breast cancer cells. We found that increased HDAC4 expression in MDA-MB-231 cells was associated with resistance to the anticancer drug 5-fluorouracil (5-FU). To verify these results, a cell line stably overexpressing HDAC4 was generated using MCF-7 cells (HDAC4OE). This cell line displayed increased 5-FU resistance, and HDAC4 knockdown in HDAC4OE cells restored 5-FU sensitivity. Consequently, we concluded that HDAC4 is a critical gene associated with 5?FU chemoresistance. Further investigation using a microarray approach revealed that 355 genes were differentially expressed following HDAC4 overexpression. Based on functional annotation of the array results, HDAC4 overexpression was found to downregulate genes related to the transforming growth factor (TGF) β signaling pathway, including SMAD4, SMAD6, bone morphogenetic protein 6, inhibitor of DNA binding 1 and TGFβ2. We also found that HDAC4 expression regulates SMAD4 expression by inducing deacetylation of histone H3 in the SMAD4 promoter region. In addition, SMAD4 knockdown in MCF?7 cells increased 5-FU resistance. In summary, our data suggest that HDAC4?mediated deacetylation of the SMAD4 promoter may lead to 5-FU resistance in breast cancer cells.</P>

Phosphorylation of carboxypeptidase B1 protein regulates β-cell proliferation

Yu, Seong-Lan,Han, Seungyun,Kim, Hong Rye,Park, Jong Woo,Jin, Dong Il,Kang, Jaeku UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.40 No.5

<P>A reduction in pancreatic islet β-cells leads to the onset of diabetes. Hence, the identification of the mechanisms inducing β-cell proliferation is important for developing a treatment course against the disease. It has been well established that post-translational modifications (PTMs) of proteins affect their functionality. In addition, PTMs have been suggested to play important roles in organ regeneration. Therefore, in this study, we investigated PTMs associated with pancreatic regeneration using two-dimensional electrophoresis. Four carboxypeptidase B1 (CPB1) proteins were identified at different isoelectric points, with the same molecular weight. The motif of CPB1 PTMs was identified by mass spectrophotometry, and the downregulation of CPB1 phosphorylation in pancreatectomy was confirmed. The dephosphorylation of CPB1 induced β-cell proliferation. We thus surmise that the altered PTM of CPB1 is associated with pancreatic regeneration.</P>

Identification of mTOR inhibitor-resistant genes in cutaneous squamous cell carcinoma

Yu, Seong-Lan,Lee, Dong Chul,Baek, Seung Woo,Cho, Do Yeun,Choi, Jong Gwon,Kang, JaeKu Dove Medical Press 2018 Cancer management and research Vol.10 No.-

<P><B>Purpose</B></P><P>The PI3K/AKT/mTOR pathway is frequently activated in various squamous cell carcinomas (SCCs). Although mTOR inhibitors are suggested as effective treatments in immunosuppressed patients with metastatic SCC, they are still not proven to be favorable in treating skin SCC patients not undergoing immunosuppressive therapy. Moreover, the exact mechanism of the mTOR signaling pathway in SCC has not yet been identified. In this study, we aimed to determine the genes associated with mTOR inhibitors in skin SCC.</P><P><B>Materials and methods</B></P><P>The identification of cell viability according to concentration of everolimus and Western blot was done. To analyze the global gene expression profiles, A431 and HSC-1 cells were treated with dimethyl sulfoxide (DMSO) or 100 nM of everolimus for 72 hours. Furthermore, differentially expressed genes (DEGs) were identified using Affymetrix analysis. To identify the gene network associated with everolimus resistance in SCC cells, pathway analysis was performed using the Ingenuity Pathway Analysis (IPA) tool.</P><P><B>Results</B></P><P>The effects of cell death with respect to the mTOR inhibitor concentration were observed in the HSC-1 cell line; however, the mTOR inhibitor did not show effective cytotoxic activity in the A431 cell line. p-mTOR concentration also diminished with respect to everolimus concentrations in the HSC-1 cell line. Moreover, the microarray results showed that the <I>MYC/CCND1/TP73/NUPR1/SBD/ERBB2/CDKN2B</I> genes were related to mTOR inhibitor resistance. However, CCND1 gene overexpression was most closely related to mTOR inhibitor resistance.</P><P><B>Conclusion</B></P><P>We identified mTOR inhibitor resistance genes, and our findings may help select therapeutic targets in skin SCC.</P>

Identification of Differentially Expressed Genes Between Preadipocytes and Adipocytes Using Affymetrix Bovine Genome Array

Seong Lan Yu,Sang Mi Lee,Man Jong Kang,Hang Jin Jeong,Byung Chan Sang,Jin Tae Jeon,Jun Heon Lee 한국동물자원과학회 2009 한국축산학회지 Vol.51 No.6

소의 체세포핵이식태반과 정상태반간의 차등 발현 유전자 분석

유성란 ( Seong Lan Yu ),정행진 ( Hang Jin Jeong ),상병찬 ( Byung Chan Sang ),류승희 ( Seung Heui Ryoo ),정기철 ( Kie Chul Jung ),윤종택 ( Jong Taek Yoon ),성환후 ( Hwan Hoo Seong ),진동일 ( Dong Il Jin ),이준헌 ( Jun Heon Lee ) 한국동물자원과학회 2008 한국축산학회지 Vol.50 No.5

돼지 유전체 염기서열을 이용한 내인성 리트로 바이러스 분석에 관한 연구

유성란(Seong-Lan Yu),이준헌(Jun Heon Lee) 충남대학교 농업과학연구소 2009 농업과학연구 Vol.36 No.2

본 연구는 현재까지 발표된 돼지의 genomic sequence 정보를 이용하여 PERV들의 정확한 삽입 위치를 파악하고 그들의 특성을 분석하고자 실시하였으며 총 2.7 Gb인 돼지 genome 염기서열 중 4.2%인 114 Mb의 염기서열에서 PERV sequence를 확인한 결과 총 8개의 PERV sequence를 확인할 수 있었다. 확인된 PERV sequence중 7개는 유전자내에 deletion이 확인되었으며 나머지 한 개의 PERV도 gag와 env 유전자에 stop codon이 확인되어 정상적인 PERV로 발현되지 않을 것으로 추정되었다. 본 연구는 돼지를 이용한 이종장기이식과 관련하여 PERV를 제어하기 위한 중요한 기초 연구 자료를 제공할 것으로 사료된다. This study was conducted to identify the PERV (Porcine Endogenous Retrovirus) integration sites and their characterizations using the porcine genomic sequence information. Total 114 Mb (4.2%) sequence of the 2.7 Gb pig genome was investigated for the PERV sequences. As the results, 8 PERV sequences were identified and their genomic structures were deduced from the BLAST searches against previously known PERV genes. Seven PERVs have internal deletions in the protein coding region and they will not be functional. The other one also has internal deletions in the gag and env genes, indicating this PERV is also defective. Even though we could not identify the functional PERVs in this study, the results presented here can be used for the fundamental research materials for controlling PERV infections in relation to xenotransplantation using porcine organs and tissues.

기준점 관리를 위한 온톨로지 모델링과 적용 방안

( Yu Lan Cui ),황현숙 ( Hyun Suk Hwang ),신성현 ( Seong Hyun Shin ),서용철 ( Yong Cheol Suh ),김창수 ( Chang Soo Kim ) 한국지리정보학회 2008 한국지리정보학회지 Vol.11 No.3

정확한 지리위치 좌표를 나타내는 기준점은 국가의 중요한 자산으로써 전국토의 측량과 기타 측량 사업에 사용되고 있다. 유비쿼터스 기술의 발전으로 위치정보는 우리 생활에서 중요한 역할을 하고 있다. 현재 RFID (Radio-Frequency Identification)와 같은 유비쿼터스 기술을 기준점 관리시스템에 융합함으로써 관리의 효율성을 제고하기 위해 여러 분야에서 연구가 진행되고 있다. 그러나 기존의 연구에서는 데이터 관점에서 기준점 관리를 위한 호환성과 효율적인 검색에 대한 연구는 미비한 실정이다. 따라서, 본 논문에서는 온톨로지 기술을 사용하여 기준점 데이터를 효율적으로 검색하기 위한 데이터 모델링을 구축하고 그의 적용 방안에 초점을 두어 연구한다. 제안된 온톨로지 기반의 검색 시스템은 계층적 검색으로 사용자의 반복된 검색 수행을 줄일 수 있고, 연관 검색으로 검색 시간을 줄일 수 있는 장점이 있다. 또한, 사용자 인터페이스와 관련된 소스 코드를 수정하지 않고 카테고리와 속성의 항목을 편집할 수 있는 효과적인 검색 시스템 구축 방법을 제안한다. The control points are important assets of countries which express the most accurate location information that is used in surveying land and other measurements. The location information has played an important role in our daily lives with the development of ubiquitous technology. While many researchers have recently applied new technology like RFID(Radio-Frequency Identification) to the effective management of control points, the research into data retrieval and the interoperability of control point data is still primitive step. Therefore, we construct a data modeling to effectively manage control points using ontology data structure and focus on semantic retrieval method. Our retrieval system can provide the inferred and associated information among data using Protege-OWL tool. Our system has advantages in reducing the number of repeated queries by hierarchy searching and improving the searching time by association searching. Also, we propose an effective method to construct retrieval systems being able to edit items of categories and properties without editing the related codes.