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( Nguyen Thi Hang ),( Nguyen Thi Bich Thu ),( Le Ba Vinh ),( Nguyen Viet Phong ),( Tran Van On ),( Ki Yong Lee ) 한국미생물생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.7
Metabolites from medicinal plants continue to hold significant value in the exploration and advancement of novel pharmaceuticals. In the search for plants containing compounds with anti-inflammatory effects, we observed that the ethanol (EtOH) extract obtained from the aerial components of Gouania leptostachya DC. var. tonkinensis Pit. exhibited substantial suppression of nitric oxide (NO) in vitro. In a phytochemical study on an EtOH extract of G. leptostachya, 11 compounds were purified, including one unreported compound namely gouanioside A (1). Their chemical structures were unambiguously determined through the use of various spectroscopic techniques, such as 1 and 2D NMR, IR, and HR-ESI-MS, and by producing derivatives via chemical reactions. The EtOH extract, fractions, and a new compound exerted inflammatory effects by altering NO synthesis in murine RAW264.7 macrophage cells stimulated with lipopolysaccharide. The underlying inflammatory mechanism of the new compound 1 was also explored through various in vitro experiments. The results of this study indicate the potential usefulness of new compound 1 from G. leptostachya as a treatment for inflammatory diseases.
Ba Vinh, Le,Jang, Hyun-Jae,Viet Phong, Nguyen,Dan, Gao,Won Cho, Kyoung,Ho Kim, Young,Young Yang, Seo Pergamon Press 2019 Bioorganic & medicinal chemistry letters Vol.29 No.16
<P><B>Abstract</B></P> <P>Chromatography of the ethanol extract of the medicinal fruit <I>Stauntonia hexaphylla</I> resulted in the purification of 26 compounds (<B>1</B>–<B>26</B>), including two undescribed triterpene saponins <B>1</B> and <B>2</B> (hexaphylosides A and B). Their structures were confirmed by spectroscopic data, including IR, HR QTOF MS, <SUP>1</SUP>H, <SUP>13</SUP>C NMR, COSY, HMQC, HMBC, and TOCSY, and HPLC sugar analysis after acid hydrolysis. The anti-inflammatory effects of the high-purity constituents (<B>1</B>–<B>26</B>) on lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells were investigated by screening nitric oxide production. The NO inhibitory activity of compounds <B>6</B> and <B>10</B> with the IC<SUB>50</SUB> values of 1.33 and 1.10 µM, respectively. The structure-activity relationships (SAR) of the isolated compounds were also analyzed. Furthermore, compounds <B>6</B> and <B>10</B> inhibited the protein expression inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 via Western blotting analysis. This showed that compounds <B>6</B> and <B>10</B> contributed to the anti-inflammatory effects of <I>S</I>. <I>hexaphylla</I> fruit, which could be developed as a natural nutraceutical and functional food ingredient.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Two new triterpene saponins <B>1</B> and <B>2</B> were established by unambiguously spectroscopic methods and chemical reaction. </LI> <LI> The ethanol extract, fractions, and isolated compounds showed significant NO inhibitory activities. </LI> <LI> Compounds <B>6</B> and <B>10</B> inhibited iNOS and COX-2 expression. </LI> <LI> The <I>S. hexaphylla</I> fruit could be developed as a natural nutraceutical and functional food ingredient. </LI> </UL> </P> <P><B>Graphic abstract</B></P> <P>[DISPLAY OMISSION]</P>
Duc Khanh Le,Quang Khai Le,Thi Thuy Hang Tran,Duc Viet Nguyen,Thi Hang Dao,Thi Thuy Nguyen,Xuan Lam Truong,Quang Cuong Nguyen,Huy Phong Pham,Thi Thu Thuy Phan,Gajendra Babu Baktavachalam 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2
Susceptibility of Asian corn borer (ACB), Ostrinia furnacalis (Guenée) to Bacillus thuringiensis (Bt) Cry1Ab protein was studied between 2015 and 2016 with 11 ACB populations, collected from various geographical regions in Vietnam. A concentration range of Cry1Ab from 0.20 to 26.10 ng/cm 2 of diet was evaluated against F 1 ACB neonates using diet surface-overlay bioassays. Mortality data was recorded daily until seven days after infestation. Growth inhibition was recorded at the end of seven days. The median lethal concentration (LC 50 ) varied ≈3-fold among the different populations, ranging from 0.58 to 1.83 ng/cm 2 of diet with an overall mean of 0.86 ng/cm 2 of diet. Even the lowest concentration of 0.20 ng/cm 2 caused 73.53% growth inhibition. > 90% growth inhibition was achieved at 0.82 ng/cm 2 or higher concentrations. The results reflect natural variation in Bt susceptibility among ACB populations rather than variation caused by prior exposure to selection pressures. LC 99 value (17.26 ng/cm 2 ) was generated by pooling mortality data across different populations. The upper fiducial limit of LC 99 (24.38 ng/cm 2 ) could be a potential diagnostic dose for future resistance monitoring programs. The findings from this study suggest that ACB populations in Vietnam are highly susceptible to Cry1Ab protein. This is the first report of Cry1Ab susceptibility of different ACB populations in Vietnam and will serve as a baseline for future resistance monitoring work.