Bacillus subtilis K-1에 의한 biopolymer의 생산과 levansucrase활성의 최적배양조건

김성호,정낙현,임무현 大邱大學校 科學技術硏究所 1996 科學技術硏究 Vol.3 No.1

In order to study on the biopolymer production and the changes of its molecular composition, optimal culture condition for biopolymer production, levansucrase activity increase using identified strain and molecular structure and conformation of produced biopolymer were investigated. The mutants which was improved production ability of biopolymer and changed levansucrase activity were derived. The optimal culture conditions for bioplymer production and levansucrase activity increase were sucrose 6%, M.S.G. 2%, NaH₂P0₄·2H₂O 0.3%, MgSO₄·7H₂O 0.05%, biotin 0.1㎍/㎖ at pH 6.0, 37℃ for 48hours. Under this conditions, biopolymer production and levansucrase activity were about 25g/ℓ, 830 unit respectively. Among the several mutants which were gaind by UV light irradition, levansucrase activity of mutant M-4 was 3 fold higher and biopolymer production of mutant M-1 was 2 fold higher than wild type strain..

노인성질환 환자의 돌봄을 위한 전반적인 상태 평가표 및 돌봄을 이한 육체적 노동 요구 평가표의 타당도 및 신뢰도

김태유,김상윤,임병훈,권오영,최낙천 대한노인병학회 2002 대한노인병학회지 Vol.6 No.4

유전실험법에 의한 가교폴리에틸렌의 절연특성 평가

김원종,임윤희,심낙순,심재선,방영근 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.1

In this paper, we estimated the insulation properties of cross-linked polyethylene (XLPE) for ultra-high voltage cable. we have studied the dielectric characteristics of XLPE due to frequency and temperature variation. The dielectric characteristics were measured in the temperature range from 25[℃] to l20[℃]. Also we measured in the voltage range of l[V] to 20[V] according to the step voltage application method. From FT-IR spectrum as an analysis of physical properties, a strong absorption in wavenumbers 700 to 730[cm^(-1)] 1456[cm^(-1)] and 2700 to 3000 [cm^(-1)] observed by the methyl group(CH₂). From the analysis of DSC. the crystalline melting points of the specimen observed in the temperature 60[℃] and 106.58[℃].

Cellulase의 利用에 關한 硏究 : 第 1 報 大豆蛋白의 抽出에 對하여 Ⅰ. On the extraction of the soybean protein

金明燦,成洛癸,奇宇京 진주농과대학 1969 진주농과대학 연구논문집 Vol.- No.8

絲狀菌 Cellulase의 食品加工에의 利用의 一環으로 大豆와 비지에 對하여 Protein의 抽出試驗을 하였으며 實地 豆腐製造에 利用하여 다음과 같은 結果를 얻었다. 1) 本實驗室에서 製造한 Trichoderma 劑 酵素는 大豆와 비지에 對하여 Protein 抽出率이 높았다. 2) Trichoderma 酵素에 混在하는 大部分의 Protease는 熱處理(60℃30分)에 依하여 Cellulase component의 많은 失活없이 除去되었다. 3) 熱處理한 同 Trichoderma Cellulase를 使用하여 大豆와 비지로 부터 豆腐를 만들어 顯著한 收量 增加를 얻었다. As the part of the utilization of celluloytic enzyme derived from Trichoderma viride to the food processing, the extractability of the protein from soy bean and soy bean refuse was studied and some practical point in the preparoion of the soy bean curd was investigated. The results obtained were summerized as follows: 1) The crude enzyme from Tricoderma viride prepared in our laboratory had high extractability of protein from both soy bean and soy bean refuse. 2) Most of protease from Trichoderma viride was eliminated with little loss of celluloytic activity by heat treatment. 3) The increased yield of soy bean curd from soy bean refuse or soy bean was obtained using heat treated crude enzyme.

유전특성에 미치는 선형 저밀도 폴리에틸렌과 에틸렌 비닐 아세테이트 혼합비 영향

김원종,정의남,임윤희,심낙순,박하용 三陟大學校 2002 論文集 Vol.35 No.-

The physical and electrical properties of electrical insulating materials due to the mixing ratio of linear low density polyethylene(LLDPE) and ethylene vinyl acetate(EVA) are studied. Specimens are selected as LLDPE and EVA of mixing ratio of 50 : 50, 60 40, 70 : 30, 80 20 and virgin. To analyze the physical properties of specimens, Fourier transform infra-red spectrum, X-ray diffraction and differential scanning calorimetry are used. To investigate the influence on dielectric characteristic due to Physical structural change of specimens, we investigated the frequency and temperature dependency of tan f for virgin specimen and mixture specimens in the temperature range of 25[t]~120['c], frequency range of 30[Hz] ~l.5×10s[Hz]. For the value of tan 3, dielectric loss, at each temperature of 35, 75, 100, 120[℃], it is confirmed that as applied voltage increase, α and β peak moved to low frequency area at high temperature area, and also moved to high frequency at low temperature areas, the width of peak widen. it is considered to be caused by the molecular vibration, reduction of side-branch density, reduction of crystalline with increasing temperature.

칸디다균에 의해 발생된 피부 궤양

김낙인,조항래,김형섭 대한의진균학회 1996 대한의진균학회지 Vol.1 No.1

Cutaneous ulcers due to candida infection are very rare condition, occurring almost in immunocompromised host or occuring as cutaneous manifestation of systemic candidal infection. A 52-year-old woman presented with cutaneous ulcer on dorsum of left hand which had lasted for about 15 days. Bacterial culture showed nonpathogenic organism, Enterobacter cloacae. Mycologic studies including KOH mount and fungus culture were positive for pseudohyphae and colonies of Candida species. A biopsy specimen from the center of ulcer revealed numerous spores and fungal hyphae in the upper dermis and necrotic epidermis. Bacterial culture and fungus culture of patient's serum and urine were negative. Initial treatment with antibiotics had no effectiveness for 2 weeks. After then we treated with antifungal drug, itraconazole 100mg/day. After 6 weeks of antifungal therapy, the skin lesion was much improved. We report this case, because it showed cutaneous ulcer due to candida infection but had neither systemic candidal infection nor clinical sign of immunosuppression.

混成 回路를 使用한 廣範圍 周波數 分周器에 관한 硏究

金將起,宋洛雲 단국대학교 1980 論文集 Vol.14 No.-

When we use a monostable Multivibrator as a frequency divider, there is always a upper frequency limit due to the recovery time circuit. To solve this problem in this paper, we coupled a chain of bistable flip-flops to the Monostable Multivibrator. Then the frequency division ratio can be increased while maintaining the variability of the Monostable Multivibrator as a frequency divrider.

살충성 O,O-Dimethyl-O-(3-Metyl-4-Nitrophenyl)-phosphorothioate (Sumithion^�)의 전기화학적 환원반응에 미치는 Micell의 영향

成洛道,明平根,朴勝熙,金日光 圓光大學校 基礎自然科學硏究所 1988 基礎科學硏究誌 Vol.7 No.3

The electrochemical reduction of sumithion in various surfactants, NaLS, CTABr, Triton X-100 and in acetonitrile solution has been examined by DC, DP polarography and cyclic voltammetry (CV). Especially, in anionic surfactant, NaLS solution, the height of reduction wave is dramatic eliminated and half-wave potentials are shifted to strong negative potential (-2.7 volt vs. Ag-AgCl) by repulsion of nitro group in sumithion and anionic micell surfaces. The processes of reduction of sumithion were irreversibly electrochemical mechanism and the result of the reaction at high cathodic potential (-2.7v. vs. Ag-AgCl), O,O-dimethyl-O-(3-methylhydoxyaminophenyl) phosphorothioate is formed as major product via O,O-dimethyl-O-(3-methylnitrosophenyl) phospho-rothioate in NaLS micell solution.

낙동강하구 생태계에서의 환경요인 분석과 질소순환에 관여하는 미생물 변화에 관한 연구

최낙현,김동식,이혜주 東亞大學校附設基礎科學硏究所 1996 基礎科學硏究論文集 Vol.13 No.2

낙동강 하구에서 질소순환에 관여하는 미생물군집의 분포와 활성의 계절적 공간적 변화와 차이를 조사하기 위하여 95년 12월부터 96년 11월까지 매월 낙동강 하구의 3개 정점을 대상으로 12가지의 환경인자와 총세균, 병원성세균의 개체수 및 질소 순환에 관계하는 개체수를 분석하였다. 염분의 분포에 있어서 정점 Ⅰ에서는 하구둑건설 이후 해수의 영향이 매우 적게 미쳐 농도가 낮을 뿐 아니라, 다른 환경요인과는 달리 변화가 가장 적다. 정점 Ⅱ와 Ⅲ은 감조수역으로 염분의 변동이 가장 심하다. 수온이 낮은 겨울철에 높은 용존산소의 분포를 나타내어 용존산소와 수온이 밀접한 관련이 있음을 보여준다. 강우량 또한 무기영양염류의 분포에 영향을 많이 미쳐 5-7월에 평균치 이상의 높은 영양염류의 농도를 나타냄을 알 수 있다. 본 실험의 결과에 의해 낙동강 하구의 질산염질소의 분포는 계절에 따라 생태계의 외부로부터 질산염 질소의 유입이 많음은 물론 질화작용에 의해서 영향을 받는 것을 알 수 있다. 질소순환에 관계되는 세균의 연중 분포가 환경인자와 무기영양염류의 영향을 받아 나타남을 알 수 있다. 또한 연중 높은 수치를 보이는 암모니화세균과 질소고정세균에 의해 토양으로부터 많은 양의 영양물질이 유입되는 것을 추측할 수 있다. 인산염인과 엽록소 a의 상관관계는 이미 잘 알려져 있다. 녹조현상이 심각했던 96년 4월과 7월사이에 인산염인과 엽록소 a의 농도가 아주 높았던 것으로 보아, 본 연구에서도 그러한 양상을 보이고 있는 것으로 추측된다. From December 1995 to November 1996, 12 variables in physico-chemical factors, bacterial populations(total bacteria, pathogenic bacteria, concern of nitrogen cycle) were investigated 12 times as 3 stations in the estuary of Naktong river. Salinity was not only low concentration which is little effect of seawater as a result of the esturay dam construction at station Ⅰ, but also which is little variation than other parameters and much variation at station Ⅱ and Ⅲ.

組織素 分解酵素를 生成하는 高溫性 곰팡이의 分離 및 特性

성낙계,강인수,박채규,정영철,김두현 慶尙大學校 1990 論文集 Vol.29 No.1

고온에서 잘 생육하고 cellulose 및 xylan 분해력 이 우수한 곰팡이를 분리할 목적으로 pH5.0, 50℃, Czapek-Dox pulp 배지에서 celluase complex와 xylanase생산이 우수한 곰팡이 H-702균주를 최종선정하여 균학적 성상을 조사한 결과, Aspergillus속과 거의 일치하였으며, 본 공시균주가 효소 생산에 미치는 영향과 효소학적 성질의 결과를 요약하면 다음과 같다. 생육 및 효소생성의 최적온도는 50℃이고, 균체증식 최적 pH는 5.0, cellulase 생산 최적 pH 는 4.5 xyanases는 4.0으로 나타났으며, 효소최대 생성 배양 4일째에, 균체증식은 약 3일경에 최대에 도달하였다.또한 α-1, 4-linkage를 가진 섬유성 물질 왕성하였으며 glucose 와 같은 저분자물질에서는 효소생합성이 현저히 저해되었는데 이런 결과로 볼때 이균주는 carbon catabolite repression을 받고 있었다. 효소생산에 적합한 탄소원으로는 α-cellulose,avicel, filter paper 등 이였으며, 질소원으로는 암모니아가 효과적이었으며 무기염류는 Ca??, Mg??, Mn??, Co??에서 효소생합성이 좋은것으로 나타났다. 계면활성제는 0.15% Tween 80이 좋았다. 개선된 KD배지에서는 각종 효소활성도가 1.2-1.6배 증가하였으며 또한 KD배지에서 각종탄소원을 지질로하여 효소 생합성을 조사한 결과 3% a-cellulose 에서 효소활성도가 좋았고 값싼 기질인폐신문지에서도 효소활성이 높게 나타났다. 조효소의 최적 온도는 cellulas complex 는 70℃, xylanase는 60℃로 나타났으며 열안정성은 cellulas complex 70℃까지는 30분간 , 80℃에서는 잔존활성이 약 93%였고 xylanase는 70℃에서도 거의 안정하였다.최적 pH는 cellulas complex는 5.0, xylanase는 5.5였으며, pH안정성은 3.0-8.0까지 안정하였다.조효소의 미치는 금속이온의 영향은 Ca??,Mg?? Mn??에의해 활성화 되었으며 Pb??, Cu??, Mg?? 에 의해 현저히 저해 현상을 나타냈다. In order to isolate a fungus which grow well in the high temperature and has the excellent decomposing power of cellulose and xylose a fungus. H-702 which produced potently cellulase complex(CMCase,avicelase and β-glucosidase) and xylanase in Czapek-Dox pulp medium (pH 5.0, 50℃) was finally selected. Then as a result of its bacteriological properties investigated it was almost the same properties as Apergillus sp.. The effect influencing to the enzyme production of the isolated strain and enzymological properties are followed. The optimal temperature and pH for cell growth and enzyme production were 50℃, 5.0, 4.5 and 4.0.respectively . The highest production of enzyme was shown when the culture time was three days and the greatest growth of cell was shown about after two days. In the cellulosic materials(CMC.avicel and α-cellulose etc.) containing α-1.4-linking the vigorous production if enzyme was shown and in the small molecular substance such as glucose was remarkably repressed. According to these results, this stain was under the control of carbon catabolite repression.α-cellulose , avicel and filter paper were suitable for carbon source in the enzyme production and NH? for nitrogen source and Ca??, Mg??, Mn?? and Co?? for inorganic salt. 0.15% Tween 80 was a good surface active agent. In improved KD medium each enzyme activity was increased 1.2 to 1.4 times. As a result of investigating enzyme biosynthesis, which each carbon source was used as substrate in the KD medium, it was shown a good enzyme activity. The optimal temperature of crude enzyme in cellulase complex and xylanase were 70 ℃and 60℃, respectively. Thermal stability was shown for 30 minutes until 70℃in cellulase complex and its residual activity at 80℃ was about 93%. It almost was stable at 70℃in xylanase. The optimal pH for cellulase complex and xylanase were 5.0 and 5.5.respectively and they were stable in the range of pH 3.0 TO 8.0. The crude enzyme was activated by Ca?? , Mg??and Mn?? but inhibited by Pb??, Cu?? and Ag?? remarkably.