혈압 및 혈관손상에 대한 Candesartan과 Cilnidipine의 병용투여 효능 평가

정상혁,한주희,명창선 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-

This study was designed to evaluate the effect of candesartan combined with cilnidipine on high blood pressure in spontaneous hypertensive rats (SHRs) and vascular injury induced by cuff-placed neointimal hyperplasia model. To measure blood pressure, low and high doses of candesartan (0.765 and 1.53 mg/kg/day) and cilnidipine (0.96 and 1.92 mg/kg/day) alone or in combination were orally administered in telemetered-SHRs for 2 weeks. To examine the protective action of vascular injury, high doses of candesartan (1.53 mg/kg/day) and cilnidipine (1.92 mg/kg/day) alone or in combination was orally administered for 2 weeks in cuff-induced neointimal hyperplasia model of C56BL/6 mice. As a result, high doses of candesartan and cilnidipine in combination decreased in systolic blood pressure (SBP) and mean arterial pressure (MAP) as compared with monotherapy of high dose of each drug, but not statistically significant. In vascular injury model, candesartan and cilnidipine in combination decreased BrdU-positive cells in neointima and vascular smooth muscle cell proliferation as compared with monotheray of each drug. Thus, these results indicate that the combination of candesartan with cilnidipine is effective for the protection of vascular injury, suggesting this combination to be a valuable tool for the management of vascular remodelling such as restenosis.

Losartan과 Amlodipine의 병용투여에 의한 고혈압 및 혈관재협착 보호효과

한주희,정상혁,명창선 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-

Abstract - To examine the protective effect of combination treatment of angiotensin II AT1-receptor blockers (ARBs) with Ca2+ channel blockers (CCBs) for high blood pressure and neointima hyperplasia, losartan and amlodipine were orally injected into telemetered-spontaneous hypertensive rats and vascular injury induced by cuff-inserted neointimal formation model in vivo. For the measurement of blood pressure, random orders of losartan 0, 4.79, 9.58 mg/kg/day, amlodipine 0, 0.48, 0.96 mg/kg/day, or higher/lower doses in combination were administered in spontaneous hypertensive rats (SHRs; n=10). For the measurement of neointimal hyperplasia, 9.58 mg/kg/day of losartan or 0.96 mg/kg/day of amlodipine in alone or combination were given for 2 weeks to cuffed C57BL/6 mice (n=6). Our results suggest that the combination therapy of high doses of losartan and amlodipine are effective in lowering systolic blood pressure (SBP) and mean arterial pressure (MAP) as compared with monotherapy of each drug, but not in combined therapy of low doses of two drugs. In protection for vascular remodeling, the combination therapy of losartan with amlodipine synergistically decreased BrdU-positive cells in neointima and DNA synthesis in [3H]-thymidine incorporation by reducing cell proliferation. Therefore, present study implies that combination of losartan with amlodipine may be effective for the treatment of hypertension and vascular remodeling such as restenosis.

Irbesartan과 Lercanidipine의 병용요법이 고혈압 및 혈관재형성에 미치는 효과

이도형, 임태완, 조은지, 박현수, 정상혁, 한주희, 병창선 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-

Hypertension is one of the major risk factor for cardiovascular disease and can lead to or make worse many complications, heart disease, atherosclerosis and diabetes. Thus, managing blood pressure effec tively using therapeutic drugs such as angiotensin II receptor blocker (ARB) and Ca2+ channel blocker (CCB) can be prevent other complications. Therefore, we investigated the synergistic effect of these two drugs, ir besartan and lercanidipine, for antihypertension, cardioprotection and antidiabetes. Irbesartan (28.74 mg/kg, 14.37 mg/kg) or lercanidipine (1.92 mg/kg, 0.96 mg/kg) alone or combination was administered to sponta-neously hypertensive rats (SHRs) and C57BL/6 mice. A two-week treatment of combination with irbesartan and lercanidipine significantly attenuated systolic blood pressure (SBP) and mean arterial pressure (MAP) compared with monotherapy. Futhermore. the combination therapy protected myocardial ischemia reperfusion injury more effectively than monotherapy. In vascular remodeling, combination therapy significantly diminished cuff -induced neointima formation and reduced serum -indllced vascular smooth muscle cells (VSMCs) pro-liferation compared with monotherapy. However, combination therapy had no significant synergistic effect of blood glucose regulation. Taken together, present study suggest that the combination therapy of irbesartan and lercanidipine may be more effective therapeutic strategy for the treatment of hypertension and related complications, myocardial infarction and arterial restenosis than monotherapy

Exploiting cDNA Microarray-Based Approach Combined with RT-PCR Analysis to Monitor the Radiation Effect: Antioxidant Gene Response of ex vivo Irradiated Human Peripheral Blood Lymphocyte

Myung-Hui Sung,Hyun-Jung Jun,Seung-Yong Hwang,Jae-Hoon Hwang,Jong-Hoon Park,Mi-Young Han,U-Youn Lee,Eun-Mi Choi,Young-Mee Park 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.3

Although ionizing radiation (IR) has been used to treat the various human cancers, IR is cytotoxic not only to cancer cells but to the adjacent normal tissue. Since normal tissue complications are the limiting factor of cancer radiotherapy, one of the major concerns of IR therapy is to maximize the cancer cell killing and to minimize the toxic side effects on the adjacent normal tissue. As an attempt to develop a method to monitor the degree of radiation exposure to normal tissues during radiotherapy, we investigated the transcriptional responses of human peripheral blood lymphocytes (PBL) following IR using cDNA microarray chip containing 1,221 (1.2 K) known genes. Since conventional radiotherapy is delivered at about 24 h intervals at 180 to 300 cGy/day, we analyzed the transcriptional responses ex-vivo irradiated human PBL at 200 cGy for 24 h-period. We observed and report on 1) a group of genes transiently induced early after IR at 2 h, 2) of genes induced after IR at 6 h, 3) of genes induced after IR at 24 h and on 4) a group of genes whose expression patters were not changed after IR. Since Biological consequences of IR involve<br/> generation of various reactive oxygen species (ROS) and thus oxidative stress induced by the ROS is known to damage normal tissues during radiotherapy, we further tested the temporal expression profiles of genes involved in ROS modulation by RT-PCR. Specific changes of 6 antioxidant genes were identified in irradiated PBL among 9 genes tested. Our results suggest the potential of monitoring post-radiotherapy changes in temporal expression profiles of a specific set of genes as a measure of radiation effects on normal tissues. This type of approach should yield more useful information when validated in in vivo irradiated PBL from the cancer patients.

Exploiting cDNA Microarray-Based Approach Combined with RT-PCR Analysis to Monitor the Radiation Effect: Antioxidant Gene Response of ex vivo Irradiated Human Peripheral Blood Lymphocyte

Sung, Myung-Hui,Jun, Hyun-Jung,Hwang, Seung-Yong,Hwang, Jae-Hoon,Park, Jong-Hoon,Han, Mi-Young,Lee, U-Youn,Park, Eun-Mi,Park, Young-Mee Korean Environmental Mutagen Society 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.3

Although ionizing radiation (IR) has been used to treat the various human cancers, IR is cytotoxic not only to cancer cells but to the adjacent normal tissue. Since normal tissue complications are the limiting factor of cancer radiotherapy, one of the major concerns of IR therapy is to maximize the cancer cell killing and to minimize the toxic side effects on the adjacent normal tissue. As an attempt to develop a method to monitor the degree of radiation exposure to normal tissues during radiotherapy, we investigated the transcriptional responses of human peripheral blood lymphocytes (PBL) following IR using cDNA microarray chip containing 1,221 (1.2 K) known genes. Since conventional radiotherapy is delivered at about 24 h intervals at 180 to 300 cGy/day, we analyzed the transcriptional responses ex-vivo irradiated human PBL at 200 cGy for 24 h-period. We observed and report on 1) a group of genes transiently induced early after IR at 2 h, 2) of genes induced after IR at 6 h, 3) of genes induced after IR at 24 h and on 4) a group of genes whose expression patters were not changed after IR. Since Biological consequences of IR involve generation of various reactive oxygen species (ROS) and thus oxidative stress induced by the ROS is known to damage normal tissues during radiotherapy, we further tested the temporal expression profiles of genes involved in ROS modulation by RT-PCR. Specific changes of 6 antioxidant genes were identified in irradiated PBL among 9 genes tested. Our results suggest the potential of monitoring post-radiotherapy changes in temporal expression profiles of a specific set of genes as a measure of radiation effects on normal tissues. This type of approach should yield more useful information when validated in in vivo irradiated PBL from the cancer patients.

Biological Evaluation of <SUP>131</SUP>I-Labelled Pyronaridine

Sang-Hyun Park,Hui-Jeong Gwon,Pradeep Kannampalli,Kyong-Cheol Ko,Myung-Woo Byun 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.4

Selection of Growing Medium for Acclimatization and Growth of in Vitro Apple Plantlets

Sang-Min Ko,Jin-Hui Lee,Myung-Min Oh 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.5

Establishment of the Korea National Health and Nutrition Examination Survey air pollution study dataset for the researchers on the health impact of ambient air pollution

Myung-Jae Hwang,Jisun Sung,Miryoung Yoon,Jong-Hun Kim,Hui-Young Yun,Dae-Ryun Choi,Youn-Seo Koo,Kyungwon Oh,Sungha Yun,Hae-Kwan Cheong 한국역학회 2021 Epidemiology and Health Vol.43 No.-

To provide a nationwide representative dataset for the study on health impact of air pollution, we combined the data from the Korea National Health and Nutrition Examination Survey with the daily air quality and weather data by matching the date of examination and the residential address of the participants. The database of meteorological factors and air quality as sources of exposure data were estimated using the Community Multiscale Air Quality model. The linkage dataset was merged by three ways; administrative district, si-gun-gu (city, county, and district), and geocode (in latitude and longitude coordinate units) based on the participants’ residential address, respectively. During the study period, the exposure dataset of 85,018 individuals (38,306 men and 46,712 women) whose examination dates were recorded were obtained. According to the definition of exposure period, the dataset was combined with the data on short-term, mid-term, and long-term exposure to air pollutants and the meteorological indices. Calculation of the daily merged dataset’s average air pollution linked by si-gun-gu and geocode units showed similar results. This study generated a daily average of meteorological indices and air pollution exposure dataset for all regions including rural and remote areas in Korea for 11 years. It is expected to provide a platform for the researchers studying the health impact of air pollution and climate change on the representative population and area, which may facilitate the establishment of local health care plans by understanding the residents’ health status at the local as well as national level.

An experimental study on the effect of team’s communication pattern on relationship between gender diversity and team cooperation

Myung-Hui Kim,Sanghyeon Sung 한국인사조직학회 2010 한국인사ㆍ조직학회 발표논문집 Vol.2010 No.1

The purpose of this study is to investigate the effects of communication network on the relationship between gender diversity and perceived team cooperation. Thereby, communication pattern is classified with centralized and decentralized communication according to the level of openness and flexibility of communication flow among group members. We hypothesized that homogeneous group would cooperate better in decentralized communication network, while heterogeneous group would more cooperate and communicate in centralized communication pattern. Two group decision making exercises were conducted to test our hypotheses. Total 273 college students in three different universities participated in this study. The first experiment was designed to measure group’s attitude and behavior in neutral condition. The second part was to assess the effects of different communication pattern on relationship between gender diversity and group behavior. Groups were divided into two categories, named “decentralized communication” and “centralized communication.” All groups were assigned with a same group task, but were ordered to follow different communication rules depending on which category they belong to. Two-dependent samples t-test was conducted to measure the treatment effect. According to the result, communication pattern significantly affected the relationship between gender diversity and cooperation. Then ANOVA test was used to compare any differences between homogeneous and heterogeneous group. Results showed that both female- and male homogeneous group cooperated better in decentralized communication environment. Different from hypothesis, heterogeneous group also cooperated better in decentralized communication setting. Even though the interaction between gender diversity and communication pattern is not salient, results of the present study proved the effects of organization specific communication pattern on diversity group’s work-related behavior.

Application of DFT Systems to in vitro Apple Plantlets at Initial Stage of Acclimatization

Sang-Min Ko,Jin-Hui Lee,Myung-Min Oh 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.10