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Park, Minjeong,Jeon, Yeji,Jang, Ho Hee,Ro, Hyun-Su,Park, Woojun,Madsen, Eugene L.,Jeon, Che Ok American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.16
<B>ABSTRACT</B><P>Prior research revealed that <I>Polaromonas naphthalenivorans</I> CJ2 carries and expresses genes encoding the gentisate metabolic pathway for naphthalene. These metabolic genes are split into two clusters, comprising <I>nagRAaGHAbAcAdBFCQEDJI</I>′-<I>orf1</I>-<I>tnpA</I> and <I>nagR2</I>-<I>orf2I</I>″<I>KL</I> (C. O. Jeon, M. Park, H. Ro, W. Park, and E. L. Madsen, Appl. Environ. Microbiol. 72:1086-1095, 2006). BLAST homology searches of sequences in GenBank indicated that the <I>orf2</I> gene from the small cluster likely encoded a salicylate 5-hydroxylase, presumed to catalyze the conversion of salicylate into gentisate. Here, we report physiological and genetic evidence that <I>orf2</I> does not encode salicylate 5-hydroxylase. Instead, we have found that <I>orf2</I> encodes 3-hydroxybenzoate 6-hydroxylase, the enzyme which catalyzes the NADH-dependent conversion of 3-hydroxybenzoate into gentisate. Accordingly, we have renamed <I>orf2 nagX</I>. After expression in <I>Escherichia coli</I>, the NagX enzyme had an approximate molecular mass of 43 kDa, as estimated by gel filtration, and was probably a monomeric protein. The enzyme was able to convert 3-hydroxybenzoate into gentisate without salicylate 5-hydroxylase activity. Like other 3-hydroxybenzoate 6-hydroxylases, NagX utilized both NADH and NADPH as electron donors and exhibited a yellowish color, indicative of a bound flavin adenine dinucleotide. An engineered mutant of <I>P. naphthalenivorans</I> CJ2 defective in <I>nagX</I> failed to grow on 3-hydroxybenzoate but grew normally on naphthalene. These results indicate that the previously described small catabolic cluster in strain CJ2 may be multifunctional and is essential for the degradation of 3-hydroxybenzoate. Because <I>nagX</I> and an adjacent MarR-type regulatory gene are both closely related to homologues in <I>Azoarcus</I> species, this study raises questions about horizontal gene transfer events that contribute to operon evolution.</P>
Functional Data Classification of Variable Stars
Park, Minjeong,Kim, Donghoh,Cho, Sinsup,Oh, Hee-Seok The Korean Statistical Society 2013 Communications for statistical applications and me Vol.20 No.4
This paper considers a problem of classification of variable stars based on functional data analysis. For a better understanding of galaxy structure and stellar evolution, various approaches for classification of variable stars have been studied. Several features that explain the characteristics of variable stars (such as color index, amplitude, period, and Fourier coefficients) were usually used to classify variable stars. Excluding other factors but focusing only on the curve shapes of variable stars, Deb and Singh (2009) proposed a classification procedure using multivariate principal component analysis. However, this approach is limited to accommodate some features of the light curve data that are unequally spaced in the phase domain and have some functional properties. In this paper, we propose a light curve estimation method that is suitable for functional data analysis, and provide a classification procedure for variable stars that combined the features of a light curve with existing functional data analysis methods. To evaluate its practical applicability, we apply the proposed classification procedure to the data sets of variable stars from the project STellar Astrophysics and Research on Exoplanets (STARE).
How to identify fake images? : Multiscale methods vs. Sherlock Holmes
Park, Minsu,Park, Minjeong,Kim, Donghoh,Lee, Hajeong,Oh, Hee-Seok The Korean Statistical Society 2021 Communications for statistical applications and me Vol.28 No.6
In this paper, we propose wavelet-based procedures to identify the difference between images, including portraits and handwriting. The proposed methods are based on a novel combination of multiscale methods with a regularization technique. The multiscale method extracts the local characteristics of an image, and the distinct features are obtained through the regularized regression of the local characteristics. The regularized regression approach copes with the high-dimensional problem to build the relation between the local characteristics. Lytle and Yang (2006) introduced the detection method of forged handwriting via wavelets and summary statistics. We expand the scope of their method to the general image and significantly improve the results. We demonstrate the promising empirical evidence of the proposed method through various experiments.
( Minjeong Park ),( Gyun-ho Jeon ) 대한주산의학회 2019 Perinatology Vol.30 No.4
The Herlyn-Werner-Wunderlich (HWW) syndrome is a rare congenital anomaly that is characterized by the triad of uterus didelphys, obstructed hemivagina and ipsilateral renal agenesis. It is often diagnosed in patient with pelvic or abdominal pain during puberty. Treatment is basically resection of obstructed vaginal septum by surgical approach. We describe two case of HWW syndrome, one is a neonate who was diagnosed as hydronephrosis and pelvic mass in postnatal ultrasonography, and the other is a 13-year-old girl with abdominal pain.
Flavobacterium croceum sp. nov., isolated from activated sludge.
Park, Minjeong,Lu, Shipeng,Ryu, Seung Hyun,Chung, Bok Sil,Park, Woojun,Kim, Chang-Jin,Jeon, Che Ok Society for General Microbiology 2006 International journal of systematic and evolutiona Vol.56 No.10
<P>A Gram-negative, non-motile, rod-shaped bacterium, designated strain EMB47(T), was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Growth was observed between 10 and 40 degrees C (optimum, 25-35 degrees C) and between pH 5.0 and 8.5 (optimum, pH 7.5-8.0). The predominant fatty acids of strain EMB47(T) were iso-C(16 : 0) 3-OH, iso-C(15 : 1) G, C(15 : 0), iso-C(15 : 0), iso-C(14 : 0) and iso-C(16 : 0) and it contained phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine as polar lipids. The G+C content of the genomic DNA was 40.8 mol% and the major quinone was MK-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB47(T) formed a distinct phyletic line within the genus Flavobacterium. The levels of 16S rRNA gene sequence similarity with respect to Flavobacterium species were below 94.7 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB47(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium croceum sp. nov. is proposed. The type strain is EMB47(T) (=KCTC 12611(T)=DSM 17960(T)).</P>