인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

Comparison of Dual Probe Bipolar Mode with Monopolar and Single Probe Bipolar Modes : Comparison of Dual Probe Bipolar Mode with Monopolar and Single Probe Bipolar Modes

Lee, Jeong Min,Han, Joon Koo,Kim,Se Hyung,Lee, Jae Young,Kim, Dae Jin,Lee, Min Woo,Cho, Gyung goo,Han, Chang Jin,Choi, Byung Ihn Korean Radiological Society 2004 Korean Journal of Radiology Vol.5 No.2

유화처리 바이오디젤이 도포된 콘크리트의 침투깊이 판정

백철 ( Baek Cheol ),김태우 ( Kim Tae-woo ),이재진 ( Lee Jae-jin ),이동윤 ( Lee Dong-yun ),한민철 ( Han Min-cheol ),한천구 ( Han Cheon-goo ) 한국건축시공학회 2017 한국건축시공학회 학술발표대회 논문집 Vol.17 No.2

This study is to provide a evaluation method for the penetration depth of emulsified refined bio diesel(ERBD)applied to a surface of the concrete by using water absorption capability of the concrete. The concrete applied with ERBD was immersed at water for 1 min., 5min., and 10 min. and then was checked the brightness with elapse of time. Test results indicated that there was clear difference between ERBD part and non ERBD part in concrete specimen after measuring the brightness until 120min.

잔디종자의 물리적 전처리가 캘러스 형성과 식물체 재분화에 미치는 효과

안나영,김용구,라하만 아티쿨,배은지,최수민,이광수,이병현,Ahn, Na-Young,Kim, Yong-Goo,Rahman, Md. Atikur,Bae, Eun Ji,Choi, Su Min,Lee, Kwang Soo,Lee, Byung-Hyun 한국초지조사료학회 2015 한국초지조사료학회지 Vol.35 No.4

잔디에 있어서 낮은 형질전환 효율의 직접적인 원인 중에 하나인 낮은 캘러스 유도율과 식물체 재분화율을 개선시키기 위하여 잔디 완숙종자의 전처리의 효과를 비교해 보았다. 살균한 잔디종자에 swelling 처리를 10분간 실시한 후, sea sand를 이용 하여 sea sand와 종자의 비율을 1:1로 한 다음, shaking 처리는 vortex speed 6 (1,000 rpm)에서 처리시간은 10분간 전처리를 실시하였을 때 무처리구에 비해 2배 이상의 배양 효율을 나타내었다. 또한 sea sand로 전처리한 잔디종자로부터 형성된 캘러스를 식물체 재분화 유도배지에 치상했을 그 효율이 훨씬 더 증가하는 것을 확인할 수 있었다. 이러한 결과는 연중 아무 때나 이용할 수 있는 장점을 지닌 잔디 완숙종자로부터 유도시킨 캘러스 세포를 이용하여 Agrobacterium으로 형질전환하는데 있어서 그 효율을 향상 시키는데 큰 기여를 할 것으로 추측된다. In order to improve the transformation efficiency of zoysiagrass by increasing the frequency of callus formation from mature seeds and plant regeneration, the effect of pre-treatment with sea sand was examined. Mature zoysiagrass seeds were given 10 min of swelling time before sea sand treatment using a sea sand and seed ratio of 1 : 1 and a vortex shaking speed of 6 (1,000 rpm) for 10 min. The seeds showed increased callus formation that was more than 2 times the rate in the control. In addition, plant regeneration efficiency was also increased when embryogenic callus induced from sea sand-treated mature seeds was cultured in regeneration medium. These results will be very helpful for improving the genetic transformation frequency of zoysiagrass, a recalcitrant monocot grass.

췌장 손상의 CT진단

이현경,이성우,오민구 동국대학교 경주대학 1998 東國論集 Vol.17 No.1

수술 또는 임상 소견으로 췌장 손상이 진단된 34명의 환자를 대상으로 하여 손상 직후 시행한 전산화단층촬영 소견을 후향적으로 분석하였다. 전산화단층촬영 소견상 췌장 실질 손상의 유무, 췌장 주위 액체 저류의 유무 및 위치, 전신막 비후, 동반된 장기 손상등을 알아 보았다. 34명의 췌장 손상 환자중 전산화단층촬영 소견상 췌장 실질내 병변이 관찰된 예는 22명(65%)이었고 췌장 주위 액체 저류가 관찰된 경우는 2명을 제외한 32명(94%)이었다. 액체 저류의 위치는 전신방강이 20예(59%)로 가장 많았고, 소낭이 16예(47%), 췌장과 비정맥 사이 14예(41%), 상장간막 정맥 및 동맥 주위 9예(26%), 위십이지장동맥 주위 1예(3%)였다. 전신막의 비후는 19예(56%)에서 보였다. 췌장 손상 환자의 35%에서 전산화단층촬영상 췌실질 손상이 관찰되지 않았고 췌장 주위의 액체 저류는 2예를 제외한 전례에서 관찰되었다. 그러므로 췌장 주위의 액체 저류를 관찰하는 것은 췌장 손상이 의심되는 환자에서 실질내의 손상 소견이 관찰되지 않거나 의심스러울 시 진단에 도움이 되는 유용한 소견으로 생각된다. We retrospectively reviewed the abdominal CT scans of 34 patients with surgically proven pancreatic injury or clinical pancreatitis after blunt abdominal trauma. The CT scans were analyzed for the pancreatic gland injury, presence and location of the peripancreatic fluid collections, Thickening of the anterior renal fascia, and associated injury. The pancreatic injury was identified on CT in 22 patients(65%), whereas peripancreatic fluid collections on CT were noted 32 patients(94%) who had pancreatic injury. The fluid was in the anterior pararenal space in 20 patients(59%), in the lesser sac in 16 patients(47%), between the pancreas and the splenic vein in 14 patients(41%), around the SMA & SMV in 9 patients(26%), and around the gastroduodenal artery in 1 patient(3%). Thickening of anterior renal fascia was seen in 19 patients(56%). Pancreatic injury was not observed on CT in 35% of patients with such injury, whereas peripancreatic fluid collections on CT were noted in 94% who had pancreatic injury. The presence of peripancreatic fluid is a helpful CT finding for the diagnosis of pancreatic injury after blunt abdominal trauma.

CT-2 부가 데이터 서비스 연구

이양원,나현식,강민구 호남대학교 산업기술연구소 1998 산업기술연구논문집 Vol.6 No.-

CT-2는 기존의 셀룰라폰이 셀단위의 영역에서 이동통신을 행하는 것과 동일한 개념의 보행자 중심의 소형 경량의 발신전용 개인 휴대용 이동통신 장치이다. 본 연구에서는 이러한 CT-2 시스템의 발전과 부가서비스 연구를 위해 CT-3의 개념인 착발신이 가능한 서비스의 제공과 전국 로밍 기능, 기지국간의 이동이 가능한 핸드오버 기능 및 FPLMTS 망을 사용하여 무선 멀티미디어의 음성/데이터/화상 서비스를 제공하기 위한 양방향 무선호출과 연계와 같은 진화방향에 대한 연구한다.

관광지 선택환경에 관한 연구

이훈구,정민의 기전여자대학 1998 論文集 Vol.18 No.-

We are in the critical condition under the IMF. Tourism industry is in the very difficult state. We must try to find a new tourism environment and a new marketing strategy. And then a nice tourism environment must also be built for helping tourists have good tourism. Especially the tourism environment directly and indirectly impacts on the destination decision and the destination image making. And also the components of tourist destination environments are composed of a complex resources, atmosphere etc. Because of each destination has its own character, there could be distinguished the image making. Therefore the decision environments in the tourist destination-the resources play a role to make characteristic of the destinations. Especially the nature and the facilities supply the spaces to use of tourist's activities as well as satisfy the tourist's need. According to this study presents the strategy for the decision environment of destination which includes the mature, the facility, the social/cultural, atmosphere environment. And the tourism environment must design for the tourist should be supposed to be natural, and so the tourist have good tourism.

다발성 말초신경병증으로 발현한 Churg-Strauss 증후군 1예

이지현,김종국,유봉구,김민정,김광수 KOSIN UNIVERSITY COLLEGE OF MEDICINE 2006 高神大學校 醫學部 論文集 Vol.21 No.1

Churg-Strauss syndrome (CSS) is defined by eosinophil-rich and granulomatous inflammation of the respiratory tract and necrotizing vasculitis affecting small to medium-sized vessels, associated with asthma and eosinophilia. It is strongly associated with antineutrophil cytoplasmic antibodies, especially the anti-myeloperoxidase type (p-antineutrophil cytoplasmic antibodies) are most often found in CSS. Neurologic involvement is common and may develops early in patients with CSS, usually manifestings as peripheral neuropathy especially mononeuritis multiplex. We report a 58-year-old woman of Churg-Strauss syndrome presented with distal asymmetric polyneuroapthy, which has been reported rarely.

점토소성 폐기물이 모르타르의 강도에 미치는 영향

이동규,한민철,한천구 청주대학교 산업과학연구소 2007 産業科學硏究 Vol.25 No.1

This study investigated recycling possibility of plastic clay waste (PCW), based on the fundamental properties of mortar using PCW as a mineral admixture. The results were summarized as following. For the properties of fresh mortar, slump flow of the cement mortar using PCW exhibited higher value than MK, except for the mortar incorporating SF and H. As the incorporating ratio increases, fluidity was decreased due to the improved viscosity resulted from increased particle contents. Air content also showed decreasing tendency, but this value could be negligible, compared with MK. For the properties of hardened mortar, the compressive strength of mortar using conventional mineral admixture showed similar tendency to existing results. For mortar incorporating 10% of PCW, it showed rather small strength than that of the plain mortar, but was comparable. It was thought that the decrease of the strength was due to the pozzolanic reaction at the age of 28 days. The tendency of flexural and tensile strength was similar to the results of the compressive strength test with the increase of incorporating ratio.

PLCM을 이용한 카오스 블록 암호화 기법

이성우,이민구,박정렬,신재호 東國大學敎 産業技術硏究院 2005 산업기술논문집 Vol.15 No.1

본 논문에서는 동력학적 특성이 좋은 PLCM을 이용한 256비트 길이의 암호 키와 32비트 데이터 블록의 카오스 블록 암호화 기법을 제안한다. 본 논문에서 제안한 기법은 256비트의 키에서 각 암호화 단계마다 사용될 32비트의 세션 키 두 개를 생성해서 PLCM의 초기 값과 반복회수를 결정하는데 사용한다. 본 제안 기법은 피드백 구조와 대치 기능의 S함수를 통해 암호 강도를 향상시켰다. 또한 실험 결과와 안전성 분석을 통해 본 제안 기법이 통계적(Statistical) 공격에 매우 강함을 보여준다. In this paper, we propose a chaotic block encryption scheme using a PLCM (Piecewise Linear Chaotic Map) having a good dynamical property. The proposed scheme has a block size of 32 bits and a key size of 256 bits. In the proposed scheme we use two 32-bit session keys generated from 256-bit key to decide the initial value and the number of iteration of PLCM. To enhance the strength of security we use a feedback structure and a S (Substitution) function. With results of test and analyses of security we show the proposed scheme is very secure against statistical attacks.