Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy

Sevil Atalay Vural,Rifki Haziroglu,Mehmet R. Vural,Ibrahim M. Polat,Arda S. Tunc 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.5

Canine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-b]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-b markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-b markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.

Effects of Dexmedetomidine Infusion During Sevoflurane Anesthesia on Otoacoustic Emissions

Mehmet İlhan Şahin,Alperen Vural,Aynur Akın,İbrahim Ketenci,Yaşar Ünlü 대한청각학회 2019 Journal of Audiology & Otology Vol.23 No.2

Background and Objectives: Knowing the ototoxic potential of the agents used in medical treatments is important for the protection of hearing. Although we have knowledge regarding some effects of dexmedetomidine, which is an anesthetic-sparing drug, its influence over the hearing system has never been studied and is obscure yet. The aim of this study is to determine the effects of intravenous dexmedetomidine application during sevoflurane anesthesia on otoacoustic emissions (OAEs). Subjects and Methods: This prospective randomized study was performed on 60 patients (34 male, 26 female, mean age: 30.6±9.2 years) who were scheduled for an elective surgery under general anesthesia and the patients were enrolled and randomly divided into 2 groups. They received dexmedetomidine (Group D) or Saline (Group S) infusion during a standardized Sevoflurane anesthesia. Transient and distortion product OAEs were measured preoperatively and postoperatively (24th hour). OAE results were compared within and between groups. Results: In group D postoperative OAEs were lower than preoperative OAEs and postoperative levels of group S, especially at low frequencies (p<0.05). Conclusions: Dexmedetomidine infusion affects the micromechanical function of cochlea especially in the low-frequency region. Dexmedetomidine should be carefully used during general anesthesia to avoid its probable harmful effects on cochlear micromechanics.

Müller Muscle-conjunctival Resection with or without Tarsectomy and Combined with Bandage Contact Lens Use in Ptosis Patients with Corneal Graft

Mehmet Serhat Mangan,Serap Yurttaser Ocak,Ece Turan Vural,Elvin Yildiz 대한안과학회 2021 Korean Journal of Ophthalmology Vol.35 No.1

Purpose: To examine the efficacy of ptosis correction with a Müller muscle-conjunctival resection with or without tarsectomy(MMCR±T), combined with bandage contact lens (BCL) use, in corneal graft patients. Methods: Seven patients with corneal grafts who underwent MMCR±T for treatment of ptosis were evaluated retrospectively. A BCL was applied to the grafts at the end of the surgery. The collected data included preoperative and postoperativevisual acuity, marginal reflex distance 1 (MRD-1), presence of Hering’s dependency by the phenylephrine test, symmetry outcomes,and complications after MMCR±T. Results: The average duration between the penetrating keratoplasty and MMCR±T was 14 months, with a follow-up timeof 10.4 months after MMCR±T. Hering’s dependency was observed in four (57.2%) patients before MMCR±T, and MRD-1 wasincreased in all patients based on preoperative phenylephrine tests. The mean preoperative MRD-1 was -0.14 ± 0.55 mm, andthe mean postoperative MRD-1 was 2.35 ± 0.89 mm (p < 0.0001). Symmetry outcomes of perfect (<0.5 mm), good (0.5–1mm), and fair (≥1 mm) were noted after MMCR±T in three, three, and one patients, respectively. During the follow-up, no obviouscorneal epitheliopathy, keratitis, or corneal graft rejection/failure were noted in any cases. BCL use was well toleratedby all patients. Conclusions: Most patients achieved good surgical outcomes with the application of the BCL to protect the graft and withthe use of the phenylephrine test and Hering’s dependency to predict the final eyelid position and symmetry. MMCR±T combinedwith BCL may therefore represent an alternative approach for correction of ptosis in patients with corneal graft.

Optimal Selection of Arm Inductance and Switching Modulation for Three-Phase Modular Multilevel Converters in Terms of DC Voltage Utilization, Harmonics and Efficiency

Ali Osman Arslan,Mehmet Kurtoglu,Fatih Eroglu,Ahmet Mete Vural 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

The arm inductance (AI) of a modular multilevel converter (MMC) affects both the fault and circulating current magnitudes. In addition, it has an impact on the inverter efficiency and harmonic content. In this study, the AI of a three-phase MMC isoptimized in a novel way in terms of DC voltage utilization, harmonics and efficiency. This MMC has 10 submodules (SM) perarm and the power circuit topology of the SM is a half-bridge. The optimum AI is adopted and verified in an MMC that has 100SMs per arm. Then the phase shift (PS) and phase disposition (PD) pulse width modulation (PWM) methods are investigated forbetter DC voltage utilization, efficiency and harmonics. It is found that similar performances are obtained for both modulationtechniques in terms of DC voltage utilization. However, the total harmonic distortion (THD) of the PS-PWM is found to be0.02%, which is slightly lower than the THD of the PD-PWM at 0.16%. In efficiency calculations, the switching and conductionlosses for all of the semiconductor are considered separately and the minimum efficiency of the 100-SM based MMC is found tobe 99.62% for the PS-PWM and 99.64% for the PD-PWM with the optimal value of the AI. Simulation results are verified withan experimental prototype of a 6-SM based MMC.

Optimal Selection of Arm Inductance and Switching Modulation for Three-Phase Modular Multilevel Converters in Terms of DC Voltage Utilization, Harmonics and Efficiency

Arslan, Ali Osman,Kurtoglu, Mehmet,Eroglu, Fatih,Vural, Ahmet Mete The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

The arm inductance (AI) of a modular multilevel converter (MMC) affects both the fault and circulating current magnitudes. In addition, it has an impact on the inverter efficiency and harmonic content. In this study, the AI of a three-phase MMC is optimized in a novel way in terms of DC voltage utilization, harmonics and efficiency. This MMC has 10 submodules (SM) per arm and the power circuit topology of the SM is a half-bridge. The optimum AI is adopted and verified in an MMC that has 100 SMs per arm. Then the phase shift (PS) and phase disposition (PD) pulse width modulation (PWM) methods are investigated for better DC voltage utilization, efficiency and harmonics. It is found that similar performances are obtained for both modulation techniques in terms of DC voltage utilization. However, the total harmonic distortion (THD) of the PS-PWM is found to be 0.02%, which is slightly lower than the THD of the PD-PWM at 0.16%. In efficiency calculations, the switching and conduction losses for all of the semiconductor are considered separately and the minimum efficiency of the 100-SM based MMC is found to be 99.62% for the PS-PWM and 99.64% for the PD-PWM with the optimal value of the AI. Simulation results are verified with an experimental prototype of a 6-SM based MMC.

Is catechol-o-methyltransferase gene polymorphism a risk factor in the development of premenstrual syndrome?

Deveci, Esma Ozturk,Incebiyik, Adnan,Selek, Salih,Camuzcuoglu, Aysun,Hilali, Nese Gul,Camuzcuoglu, Hakan,Erdal, Mehmet Emin,Vural, Mehmet The Korean Society for Reproductive Medicine 2014 Clinical and Experimental Reproductive Medicine Vol.41 No.2

Objective: The objective of this study was to investigate whether there was a correlation between catechol-o-methyltransferase (COMT) gene polymorphism, which is believed to play a role in the etiology of psychotic disorders, and premenstrual syndrome (PMS). Methods: Fifty-three women with regular menstrual cycles, aged between 18 and 46 years and diagnosed with PMS according to the American Congress of Obstetrics and Gynecology criteria were included in this study as the study group, and 53 healthy women having no health problems were selected as the controls. Venous blood was collected from all patients included in the study and kept at $-18^{\circ}C$ prior to analysis. Results: There was no significant difference between the groups in terms of demographic features such as age, body mass index, number of pregnancies, parity, and number of children. No statistically significant difference was observed in terms of COMT gene polymorphism (p=0.61) between women in the PMS and the control groups. However, a significant difference was found between arthralgia, which is an indicator of PMS, and low-enzyme activity COMT gene (Met/Met) polymorphism (p=0.04). Conclusion: These results suggested that there was no significant relationship between PMS and COMT gene polymorphism. Since we could not find a direct correlation between the COMT gene polymorphism and PMS, further studies including alternative neurotransmitter pathways are needed to find an effective treatment for this disease.

Prevalence of thromogenic gene mutations in women with recurrent miscarriage: A retrospective study of 1,507 patients

( Adnan Incebiyik ),( Nese Gul Hilali ),( Aysun Camuzcuoglu ),( Hakan Camuzcuoglu ),( Halit Akbas ),( Avni Kilic ),( Mehmet Vural ) 대한산부인과학회 2014 Obstetrics & Gynecology Science Vol.57 No.6

ObjectiveThromogenic gene mutations has been thought to be associated with recurrent pregnancy loss in women in Turkey. The aim of this study was to investigate the prevalence of thromogenic gene mutations such as factor V Leiden (FVL, G1691T), prothrombin (G20210A), and the methylene tetrahydrofolate reductase (MTHFR, C677T) mutation in women with recurrent pregnancy loss. MethodsThis descriptive study was carried out in the Department of Obstetrics and Gynaecology, Harran University School of Medicine, and included a total of 1,507 women with histories of recurrent pregnancy loss between January 2010 and June 2013. The mutations were assessed by using the polymerase chain reaction. ResultsThe homozygous mutation frequencies of FVL, prothrombin, and MTHFR were found to be 3 (0.20%), 0 and 125 (8.29%), and the heterozygous mutation frequencies were 83 (5.51%), 61 (4.05%), and 612 (40.61%), respectively. Among the 86 FVL mutation patients, 38 also had accompanying prothrombin and MTHFR mutations. ConclusionSince the homozygous forms of the FVL-prothrombin gene mutations have low incidences and MTHFR mutation is similar to a healthy population, preconceptional thromogenic gene mutations screening seems to be controversial.