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RISS 인기검색어
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- 저자 : ( Limin Shen ) (검색결과 5 건)
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Limin Cao,Jin Si,Weiyu Wang,Xiaorong Zhao,Xiaomei Yuan,Huifen Zhu,Xiaolong Wu,Jianzhong Zhu,Guanxin Shen 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
Gene therapy with nonviral vectors using the suicide gene/prodrug activating system of herpes simplex virus type-1 thymidine kinase (HSV1-TK)/ganciclovir (GCV) is inefficient in killing malignant tumor cells due to two major factors: (a) an unsatisfactory bystander effect; (b) short-lived expression of the protein. To study the capacity of the protein transduction domain (PTD) of HIV-1 TAT protein to enhance HSV1-TK/GCV cancer gene therapy, we constructed three fusion proteins TAT-TK, TK-TAT and TK. TATTK retained as much enzyme activity as TK, whereas that of TK-TAT was much lower. TAT-TK can enter HepG2 cells and much of it is translocated to the nucleus. The transduced HepG2 cells are killed by exogenously added GCV and have bystander effects on untransduced HepG2 cells. Most importantly, the introduced recombinant protein is stable and remains functional for several days at least, probably because nuclear localization protects it from the cytoplasmic degradation machinery and provides access to the nuclear transcription machinery. Our results indicate that TAT fusion proteins traffic intercellularly and have enhanced stability and prodrug cell killing activity. We conclude that TAT has potential for enhancing enzyme prodrug treatment of liver cancers.
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Effects of Huzhangoside C on Dextran Sodium Sulfate-Stimulated Colitis in Mice
Limin Chen,Yonghong Zhang,Jinhuang Shen,Ying Wu,Fan Cao,Hongying Hua,Xinhua Ma 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.1
Chronic inflammation is a major risk factor for cancer. Inflammatory bowel disease (IBD) is a chronicinflammatory disease of the gastrointestinal tract, ultimately leading to a breakdown of intestinal barrier function. Clematisflorida var. plena is a folk prescription used to treat inflammation and rheumatism in She pharmacy. The bioactivity of C. florida var. plena is primarily due to triterpene saponins. Huzhangoside C (HZ) is an active component of C. florida var. plena. In this study, the anti-inflammatory effect of HZ on a mouse colitis model induced by dextran sulfate sodium (DSS) wasinvestigated. Result indicated a notable reduction in body weight loss and colon length shortening in HZ-mediated micecompared to DSS-stimulated control mice. Furthermore, inflammatory signaling mechanisms involving interleukin-6 andtumor necrosis factor-a were suppressed in HZ-treated mice. HZ treatment significantly suppressed the expression of nuclearfactor kappa B (NF-jB), STAT3, and iNOS in colon tissue. After HZ treatment, malondialdehyde and nitric oxide levels weresignificantly decreased, while Nrf-2, superoxide dismutase, and glutathione expression levels were notably improved. Theresult indicated that HZ could activate the Nrf-2 signal cascade, inhibit the expression of NF-jB, eNOS, and STAT3, andenhance the intestinal barrier function of DSS stimulated ulcerative colitis intestinal injury. The results suggest that HZ ispotential anti-inflammatory agent for treating IBD.
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Fast transient response based on digital single‑cycle charge regulation (SCCR) control
Lingyun Li,Shen Xu,Limin Yu,Weifeng Sun 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9
The Buck converter is a commonly used voltage regulator (VR) structure that supplies power to the load devices and responds to load transients. In most of the applications, the VR is controlled by voltage or current mode control, and some nonlinear approaches are used to improve the transient response which brings more complexities to the closed loop design. To achieve a fast transient response without over-regulation under a load step transient, this paper proposes a digital single-cycle regulation (SCCR) controller, which comprised a fast path, a slow path, and a neutralization path. Unlike the conventional PID control, the neutralization path of the SCCR controller is used to offset the excessive energy change in the first cycle after a load step occurs, which makes the inductor current reach its new static condition in a shorter period of time. The mathematical model of the SCCR control is established in this paper and a scanning algorithm is proposed for the design of the compensator parameters. Experimental results are presented to verify the improvement of the transient response when compared to the conventional and nonlinear PID control, the recovery time is decreased by 64.3% and 43% for load step-up and step-down when compared with nonlinear PID control.
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An Anomalous Behavior Detection Method Using System Call Sequences for Distributed Applications
( Chuan Ma ),( Limin Shen ),( Tao Wang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.2
Distributed applications are composed of multiple nodes, which exchange information with individual nodes through message passing. Compared with traditional applications, distributed applications have more complex behavior patterns because a large number of interactions and concurrent behaviors exist among their distributed nodes. Thus, it is difficult to detect anomalous behaviors and determine the location and scope of abnormal nodes, and some attacks and misuse cannot be detected. To address this problem, we introduce a method for detecting anomalous behaviors based on process algebra. We specify the architecture of the behavior detection model and the detection algorithm. The anomalous behavior detection and analysis demonstrate that our method is a good discriminator between normal and anomalous behavior characteristics of distributed applications. Performance evaluation shows that the proposed method enhances efficiency without security degradation.
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A Process Algebra-Based Detection Model for Multithreaded Programs in Communication System
( Tao Wang ),( Limin Shen ),( Chuan Ma ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.3
Concurrent behaviors of multithreaded programs cannot be described effectively by automata-based models. Thus, concurrent program intrusion attempts cannot be detected. To address this problem, we proposed the process algebra-based detection model for multithreaded programs (PADMP). We generate process expressions by static binary code analysis. We then add concurrency operators to process expressions and propose a model construction algorithm based on process algebra. We also present a definition of process equivalence and behavior detection rules. Experiments demonstrate that the proposed method can accurately detect errors in multithreaded programs and has linear space-time complexity. The proposed method provides effective support for concurrent behavior modeling and detection.
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