남한강 상류수계 박대천의 어류상

박경서,문운기,홍영표,최신석 忠南大學校 環境問題硏究所 2001 環境硏究 Vol.19 No.-

남한강 상류 수계, 박대천 일대를 대상으로 2001년 4월부터 9월까지 총 11개 지점을 대상으로 조사하였으며, 종조성, 어류상과 군집 구조를 분석하였다. 총 7과 25속 33종으로 1570개체가 조사 기간동안 확인되었다. 전체 어류군집 중, 잉어과 어류가 24종으로 전체의 72.7%와 1531개체로 전체의 97.5%를 차지하였다. 전체 종에 대해 0.2%보다 적은 종은 L. andersoni, G. brevibraba, A. koreensis, C. rotundicaudata와 S. microdorsalis를 포함한 11종이었다. 박대천 수계에서 우점종은 상대풍부도가 28.66%로 Zacco temmincki가 우점하였고, Zacco platypus (28.28%)와 C. splendidus (12.55%)가 아우점하였다. 박대천의 각 지류에서, Zacco temmincki는 6개 지점에서, Zacco temmincki는 5개 지점에서 우점하였다. 박대천 수계로부터 총 33종 중 6과 14속 18종(54.5%)이 한국고유종이었다. 박대천에서 확인된 어류의 전체 군집 구조를 분석한 결과, 우점도 3.766, 다양도 0.903, 풍부도 10.012, 그리고 균등도는 0.594이었다. Sampes of the Bakdae-Cheon Stream, the upper Namhan-Gang River of Korea were collected at 11 stations from April to September 2001, and analyzed for the species composition, fish fauna, and species diversity. A total of 33 species (1570 individuals) and 25 genera belonging to seven families were collected during the study period. Cyprinid fishes occupied 72.7% of total 24 species and 97.5% of total 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were 1531 individuals, respectively. The rare species which relative abundance is less than 0.2% of all were about 11 species including Liobagrus andersoni, Gobiobotia brevibraba, Acheilognathus koreensis, Cobitis rotundicaudata, and Silurus microdorsalis. Dominant species in the Bakdae-cheon Stream was Zacco temmincki (relative abundance 28.66%), and subdominant species were Zacco platypus (28.28%), and Coreoleuciscus splendidus (12.55%). In each trivutaries of the Bakdae-cheon Stream, Z. temmincki was dominant species at six stations, and Z. platypus was dominant at five stations. Eighteen Species and 14 genera belonging to six families (51.5%) of total 33 species from the Bakdae-cheon Stream were well known as Korean endemic species. The species dominance (λ), diversity (H'), richness (RI), and evenness index (EI) of the fish community were 3.766, 0.903, 10.012, and 0.594, respectively.

대학교 가정학 교양과목의 교과내용구성과 수업운영방식 : Focused on Child and Family, Clothing and Textiles, Food and Nutrition, and Housing 아동가족·의생활·식생활·주생활 영역을 중심으로

박경옥,이소은,김은영,김운주 충북대학교 교육·생활연구소 1999 생활과학연구논총 Vol.2 No.-

The purposes of this study were to: (1) analyze students' educational needs and attitude concerning various areas in Home Economics as a college-level liberal education curriculum, (2) provide data concerning future curricula and efficient teaching method. Four areas of Home Economics were investigated: child and family, clothing and textiles, food and nutrition, and housing. The subjects of this study were 478 students of Chungbuk National University, divided by experiences in enrollment of Home Economics and teaching methods (managed by 1-2 teachers vs. team teaching by 4 teachers). The data were collected by questionnaires developed by the researchers and analyzed by frequencies, t-test and x²-test The major findings were as follows: 1) Child and family education, especially the subjects of child-rearing and parents education, was most needed area by students, and this area also got highest score in attitude. 2) In the field of clothing and textiles, the subject of impression management with clothing was needed the most, and the importance of clothing got the highest score in attitude. Also, the students enrolled Home Economics showed more educational needs and practical attitude. 3) In the field of food and nutrition, food safety and sanitation were the most needed subject and safety of foods making got the highest score in attitude. The students enrolled Home Economic showed more educational needs on the subjects of food safety and sanitation, human nutrition and health maintenance and the culture of food and nutrition. 4) Educational need of the students was the lowest in housing area, though the subjects of knowledge on the space planning such as interior design, use of living space, and indoor environments-heat, light and air got most attention. Educational needs and attitudes of the students were affected by student's sex, major (human vs. scientific), and age. 5) Educational needs and attitudes of the students were affected by sociodemographic variables, such as grade, sex, major (human vs. scientific), and age. And teaching method was also found to be an influential factor.

상시관측소를 이용한 Single RTK-GPS 정밀도 비교

박운용,차성렬,배경호 東亞大學校建設技術硏究所 2004 硏究論文集 Vol.28 No.1

The plenty of availability and high precision of GPS CORS is the reason why it become important more and more in the fields of surveying widely. In this study. I extracted the arbitrary point's coordinate which is using GPS CORS data. now served in RINEX FORMAT via Inter-Net, with observation network of the existing triangulation and GPS CORS data. Then. with this arbitrary point as reference station. RTK GPS was performed. And I will study VRS-GPS concept which reduces the time and cost in the fields of surveying.

배양 조건이 Candida albicans의 phospholipase 생성에 미치는 영향

신운섭,이경호,박주영,고춘명 대한의진균학회 1997 대한의진균학회지 Vol.2 No.2

Background : The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathoenesis of Candida infections. Objective : In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans. Methods : C. albicans ATTCC 10231 was cultivated in various media at 37℃ for 3 days. Phospholipase activity was measured by fatty acid soap precipitaiton in plate containing 0.04T lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar. Results : Phospholipase was highly induced when C. albicans was cultivated in broth medium(containing glucose 2%, albumin 0.2% and Fe^++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate. Conclusion : Highly induction of secreted phospholipase by albumin from C. albicans may be play an importans role in tissue invasion in the pathogenesis of C. albicans.

스포츠 참가자의 라이프스타일 특성과 서비스 만족의 관계

윤승원,정경회,이일재,박영표 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.1

The analysis of the customers' mind is crucial in the modern society where marketing strategy is very important for the satisfaction of the customers. the purpose of this study was investigating the relationship between lifestyle characteristics and service satisfaction of sports center customer. To achieve this purpose, the population of this study are the participants of the swimming pool, bowling center, fitness club and golf club among the commercial sports center in Jeonju, Jeollabugdo. They are chosen by the multi-stage stratified random sampling considering the events and region. The valid samples are 420 persons at last among 500 persons except the 80 persons whose validity are determined low. To analyze the data, frequency analysis, factor analysis, t-test, ANOVA, regression analysis and covariance structural analysis are used in this study. In this study, those following results are gathered by analysing the data through above approaches. First, According to the background variation of the sports participator; material service to income, personal service to educational level and occupation, systematic service to sex and educational level, there are significant service satisfactions. Second, Lifestyle characteristics of the sports participator influence on service satisfactions significantly; persuit of the trend, social contact, nervousness and individualism influence on personal service social contact, persuit of the trend, nervousness and sentimentalism on systematic service and the other variations on material service.

음낭외상시 고환파열에 대한 초음파 검사의 진단적 유용성

김경영,한동준,신대은,노준,문형윤,임동훈,김철성,박성운 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.3

Purpose: We determined the use of scrotal ultrasonography in the initial diagnosis and management of testicular injuries due to blunt scrotal trauma. Mateiials and Methods: We reviewed 54 patients of scrotal trauma in the last 8 years. Forty patients were evaluated by ultrasonography preoperatively. A heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition was the basis for diagnosis of testicular rupture. Among them, 33 cases underwent scrotal exploration and 7 cases were managed conservatively. The remaining 14 patients were evaluated only by physical examination, 8 patients of them were explored immediately because of suspicious testicular mpture. The accuracy of ultrasonography were determined by comparing radiographic interpretations with intraoperative findings. Results: Among the 33 patients who were evaluated by ultrasonography and explored immediately, there were 26 suspected testicular ruptures on ultrasonography. Surgical exploration revealed testicular rupture in 23 and simple hematoma in 10. The testicular rupture was correctly diagnosed by ultrasonography in the 22 cases, and there were 4 false-positive and 1 false-negative diagnoses of testicular rupture. The sensivity and specificity for the diagnosis of testicular rupture are 96% and 60%, and the positive and negative predictive values are 85% and 86%, respectively, Conclusions: Scrotal ultrasonography, with the main radiographic finding of a heterogeneous echo pattern of the testicular parenchyma with loss of oval shape, contour definition, is highly sensitive on the diagnosis of testicular rupture. And it increases the salvage rate for testicular rupture with preservation of the testicular function and prevents delayed complications of chronic pain, atrophy and orchiectomy associated with missed testicular rupture.

흰쥐에서 심정지후 Pentoxifylline이 뇌의 허혈-재관류 손상에 미치는 효과

정시경,김영민,오동렬,최경호,박승현,이운정,박규남,유은영,이원재,김세경 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Two major events occurring in the cerebral hemodynamics after successful resuscitation from cardiac arrest are reactive hyperemia and postischemic hypoperfusion. In this study, we examined the effect of Pentoxifylline(PTX) on the rat brain following cardiac arrest. Methods: Fourteen rats were anesthetized and artificially ventilated. Cardiac arrest was produced by chest compression and clamping of tracheal tube for 3 minutes in ketamine anesthetized rats. Circulation was restored by standard cardiopulmonary resuscitation methods. In 7 rats, PTX 10mg/kg was infused at 10min after cardiac arrest(PTX group). In the other 7 rats, same amount of normal saline was infused(control group). Results: In both groups, hemodynamic variables, neurologic deficit(ND) score and histopathologic findings of hippocampal CA1 neurons were observed. Hemodynamic variables and ND score were not significantly different between two groups. Delayed ischemic neurons of hippocampal CA1 were decreased in PTX group(2.2±2.4%) compared with control group(9.1±1.2%). Conclusions: We conclude that PTX prevented development of delayed ischemic neurons in hippocampal CA1 after cardiac arrest. PTX may be useful in emergency situations following cardiac arrest.

Coxiella burnetii에 대한 항체의 검출을 위한 Enzyme-Linked Immunosorbent Assay의 정립

이경호,박주영,박윤선,김수기,신운섭,고춘명 대한감염학회 1994 감염 Vol.26 No.4

본 연구에서는 지금까지 진단이 내려지지 않았던 C. burnetii 감염증 환자들을 보다 빠르고 간편하며 정확하면서도 대량으로 검사할 수 있는 방법으로서 C. burnetii 단백항원을 이용한 효소면역측정법을 정립하고자 하였다. 본 실험에서 정립한 효소면역측정법은 간접 방법으로서 C. burnetii phase I에 대한 IgG를 검출하기 위해 고안되었다. C. burnetii 단백질 항원의 적정 농도는 야성 대조혈청의 흡광도와 음성 대조혈청에서의 흡광도의 차이가 가장 크게 나는 농도인 1ug/ml을 적정항원농도로 결정하였으며, 검사 혈청의 적정희석 농도도 역시 양성대조항원의 OD 값과 음성대조항원에서의 OD값의 차이가 가장 크게 나면서 비교적 background activity가 적은 혈청의 희석 농도인 1:300을 적정 혈청 희석 농도로 결정하였다. 이 때 각각의 야성 혈청이 0.185의 흡광도를 가질 수 있는 가장 높은 혈청 희석 농도의 로그값과, 같은 혈청을 1:300으로 희석했을 때의 흡광도 사이에 상관계수 r값이 0.95인 직선적 관계가 성립하여, 하가지 혈청 희석 농도(1:300)에서 결정된 ELISA값이 그 혈청의 항체가를 양적으로 표시하였다. 효소면역측정법의 양성 판정 기준은, IFA에서 C. burnetii에 대한 항체가 음성으로 확인된 혈청 100개를 대상으로 ELISA를 시행하여, 이들의 흡광도의 산술평균치와 표준편차를 구한 결과, 신뢰구간 95%에서 최고치인 0.185를 ELISA 야성 판정의 기준인 cut-off value로 정하였다. 위애서 정한 기준에 의해 ELISA를 시행하엿을 때, IFA에서 양성으로 판명된 혈청 모두에서 양성으로 나타나 민감도가 매우 높은 것으로 나타났다. 다음으로 ELISA 방법의 특이도를 조사하기 위해서, IFA 검사에서 음성으로 확인된 혈청(혈청 희석 농도 1:10에서 음성)을 대상으로 ELISA를 시행한 결과, blocking agent로서 1% BSA를 사용했을 때는 9.3%, 5% NGS는 11.3%, 그리고 blocking을 전혀 하지 않았을 때는 15.3%의 위양성율을 나타내어, BSA와 NGS 상이에는 통계학적으로 차이가 없었으나, blocking을 하지 않는 것 보다는 하는 것이 위양성율이 적은 것으로 나타났다. 그러나, 이것은 어디까지나 IFA기준이며, 절대적인 의미에서의 위양성율을 의미하는 것은 아니다. 결론적으로 본 연구에서 정립한 효소면역측정법은 매우 높은 민감도를 갖고 있으므로 Q fever가 의심되는 환자를 screening하기 위한 적절한 방법으로 사료된다. Currently, the indirect immunofluorescence assay(IFA) is used for detecting antibodies to Coxiella burnetii. Although reliable, this method is time-consuming, and the results are subject to interpretation. To develop an alternative test, we used an enzyme-linked immunosorbent asssy (ELISA) because it was faster, less complicated, and more objective than the IFA. The ELISA, unlike the IFA, can also be automated. In this study an indirect ELISA using C. burnetii protein antigens was developed to detect immunoglobulin G to C. burnetii phase I. A linear relation was found between the logarithms of absorbance values of sera at a dilution of 1/300 and the titres as determined by an end point dilution ELISA. The finding of linear relation (r=0.95) confirmed that an ELISA value determined with a single serum dilution (1:300) can be interpreted quantitatively as the titer of antibody in that serum. A serum dilution of 1:300 was used in all subsequent tests because at this dilution the difference between absorbance values for positive and negative sera was greatest: a serum sample was regarded as positive if the OD? value was ≥0.185 (absorbance threshold) at this dilution. The results obtained by the ELISA were compared with those by the IFA established already. The results indicate that the assay provides a sensitive, alternative method for diagnosing Q fever, but it needs reevaluation for the specificity because of its high false-positive rates.

금강 상류수계 봉황천 및 주자천의 어류상

문운기,박경서,최신석,홍영표 忠南大學校 環境問題硏究所 2001 環境硏究 Vol.19 No.-

용담댐의 호소화에 의한 어류군집 변화를 알아보기 위하여 2001년 5월부터 8월까지 봉황천과 주자천을 중심으로 어류상 및 어류군집 양상을 조사하였다. 조사 결과 본 지역의 하천형은 Aa, Aa-Bb형이었다. 본 조사에서 6과 17속 19종이 출현하였다. 봉황천에서는 12종 464개체, 주자천에서는 14종 682개체가 채집되었다. 각 하천에서 우점종은 Zacco temmincki, 아우점종은 Zacco platypus이었다. 한국 고유종은 전체 19종 중 10종이었고, 전체 어종수의 52.63%로 매우 높은 비율을 보였다. 종의 다양도는 봉황천에서는 지점 4가, 주자천을 포함한 본류수계에서는 지점 9와 10이 높게 나도, 균등도와 우점도는 각각 0.616, 5.884, 0.482, 0.410이었다. 특히, 댐의 건설에 따라 댐 형성지점에서 Z. platypus와 Opsarlichthys bidens의 집단증가가 주목되었다. Formaing a part of fish community, the fish fauna species composition, and species diversity were investigated in the Bonghwang-Cheon and Juja-Cheon Stream, the upper Kum-Gang River of Korea from May to August in 2001. In the most of sampling area, the type of streams were Aa and Aa - Bb. As a whole, the collected fishes were classified 19 species and 17 genera belonging to six families. Twelve species, 464 individuals in the Bonghwang-Cheon Stream, and 14 species 682 individuals in the Juja-Cheon Stream were collected. In each stream, dominant and subdominant species was Zacco temmincki and Zacco platypus. Ten korean endemic species (52.63) were collected during the sampling period. Diversity(H'), richness(RI), evenness(EI), and dominance index(λ) of the fish communities were 0.616, 5.884, 0.482 and 0.410, respectively. By the means of physical enviroment change by Yongdam Dam construction, increasing of Z. platypus and Opsarichthys bidens population was remarked.

흰쥐 복직근과 대흉근 근섬유 유형에 대한 조직화학적 연구

민병운,박경수,노영복 조선대학교 기초과학연구소 1989 自然科學硏究 Vol.12 No.1

포유동물인 흰쥐의 대흉근과 복직근에 대한 근섬유 특성 및 근섬유 유형별 빈도를 조사하기 위하여 1988년 7월 2일~1988년 10월7일 조직화학적 및 각종 효소조직화학적 염색을 시행하여 조사한 바 다음과 같은 결론을 얻었다. 1. H-E 염색에 의한 근섬유의 유형별 구분은 불 명확하였으나, PAS 염색에서는 양성 반응의 정도에 따라서 세가지 유형으로 구분되었다. 2. Reversed myosin-ATP ase 염색과 SDH염색에서는 TypeⅠ, Ⅱa, Ⅱb의 세가지 유형의 근섬유형이 구분되었다. 그러나 typeⅡ와 Ⅱb의 유형별 빈도는 SDH염색과 Reversed myosin-ATP ase 염색상 상당한 차이가 있는 것으로 관찰되었다. 3. 포유동물인 흰쥐의 복직근과 대흉근의 근 섬유형 구성비는 부위에 따라서 상당한 차이가 있었다. In order to study properties and individual proportions of muscle fiber types on the pectoralis major an rectus abdominis muscle of the rat, vertebrates, histological and each kinds of enzyme histochemical stain (were performed). The following results were obtained : 1. Differentiation of muscle fiber types was not evident in H & E stained specimen. However, three types of muscle fibers were evidently differentiated in PAS stained sections according to the degree of positivity. 2. Muscle fiber of three types (typeⅠ, Ⅱa and Ⅱb) were differentiated in reversed-ATP ase and SDH stain. However, there were considerable difference between SDH and reversed-ATP ase stain properties in proportion of typeⅡa and Ⅱb. 3. The constituent proportions of muscle fibers in rectus abdominis and pectoralis major muscle of the rat were remarkably different according to the portions.