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표재성(表在性) 진균(眞菌)의Thiocarbanilide(L-1) 처리효과에 대한 전자현미경적(電子顯微鏡的) 관찰
고춘명,유준,김태원 대한미생물학회 1970 大韓微生物學會誌 Vol.5 No.1
The present study is of ultra-fine structures of Trichophyton rubrum, Trichophyton mentagrophytes, microsporum canis and Epidermophyton floccosum by means of electron microscopy and reveals the following; 1. In contrast to the bacteria, the normal fungus contains nucIear membrane, mitocondria, endoplasmic reticulum, distinct cell wall and cell membrane and secretory granules as observed in the higher plants and animals. 2. Thiekening of the cell wall, inapparent cell membrane with the appearance of electron thin area (ETA) and increase of inclusions were observed in the L-1 treated groups. 3. Thiekening of cell wall and increase of ETA were more apparent in the Epidermophyton floccosum than the other groups. 4. Inerease of electron thin area was thought to be associated with autolysis.
고춘명,최태주,류준,Koh, Choon-Myung,Choi, Tae-Joo,Lew, Joon 한국미생물학회 1973 미생물학회지 Vol.11 No.2
Thirtythree strains of the Aspergillus spp. isolated from foodstuffs were observed through some physiological characteristics for detection of identification key of Aspergillus spp. 1) Each strain of Aspergillus spp. had their specific characteristics and could be used for identification of species. 2) Excellent amylase-producing fungi were observed among the isolated strains of Aspergillus spp. 3) Amylase activities increased for one week incubation period. 4) In the tests of common characters of aflatoxin-producing fungi among the 33 strains of Aspergillus spp., for example, conidial size, presence of sclerotia, kojic acid, and pigment production, coloration of phenol, reduction of methylene blue, etc.
고춘명,최태주,류준,Koh, Choon-Myung,Choi, Tae-Joo,Lew, Joon 한국미생물학회 1972 미생물학회지 Vol.10 No.3
한국 토괴균의 계속적인 연구로서, 분리된 균류중 Aspergillus 8종과 microfungi 5종인 Alternaria alternata, Chrysosporium pannorum, Doratomyces microsporus, Trichoderma koningii, T. viride에 관하여 보고하였다. 이들 동정된 균류 중 아직까지 한국에서 미기록된 것으로는 Chrysosporium pannorum, Doratomyces microsporus, Trichoderma koningii, T. viride 등의 4종이었다. 또한 완전연대인 Emericella nidulans var. nidulans에 관하여 보고하였다. Fourteen species of the hyphomyceteous fungi isolated from Korean soils are described and illustrated. Among these, one species has teleomorphic state and is identified as Emericella nidulans var. nidulans, similar to Emericella spectabilis with the exception of size of the conidiophores as well as color and the arrangement of the hulle cells. Four species of hyphomyceteous fungi. Chrysosporium pannorum, Doratomyces microsporus, Trichoderma Roningii, T. viride, are reported here for the first time in Korea.
Coccidioides immitis의 mycelial phase와 tissue phase에 있어서의 전자현미경적 관찰
고춘명,최태주,등영건,류준,Koh, Choon, Myung,Choi, Tae-Joo,Deung, Young-Kun,Lew, Joon 한국미생물학회 1971 미생물학회지 Vol.9 No.4
Ultrastructural observations of mycelial and tissue phase with dimorphic fungal pathogen Coccidioides immitis were studied by electron microscopy of thin sections. 1. In mycelial phase of C.immitis contains normal cell components such as nucleus, mitochondria, endoplamsic reticulum, intracytoplasmic membrane system, cell wall and cell membrane as observed in the other encaryotic cells. 2. In tissue phase of C. immitis was larger than mycelial phase in cell size and observed much more vacuoles than mycelial phase. 3. In the contrast of mycelial phase of C. immitis, the tissue phase of cells were observed fibril form of capsular layer.
고춘명,이봉기,등영건 한국미생물학회 1977 미생물학회지 Vol.15 No.1
Bdellovibrio sp. is an ectoparasitic bacteria which is predatory and parasitic upon other bacteria. This study was carried out the isolation of Bdellovibrio sp. from several soil smaples and observation of this organisms by means of electron microscope. The results are as follows ; The primary isolated Bdellovibrio sp. from soil is an obligate intracellular rod form parasite and possess a monoflagella.
Mycotoxin분비 수종균주들의 배양여과액으로 처리된 HeLa세포의 전자현미경적 관찰
고춘명,최대경,등영건 한국미생물학회 1974 미생물학회지 Vol.12 No.3
The fine structure of HeLa cells treated with several mycotoxin-producing fungi (Aspergillus flavus ATCC 15517, Aspergillus parastiticus RIB 1037, Penicillium toxicarium RIB 4002, Penicillium cirinum SWU)238, Penicillium islandicum IFO 5235, Penicillium tadum IFO 5787 and Pencillium brunneum RIB 1172) has been examined and some details have been descried. The normal HeLa cell have numerous microvilli, large ovoid nucleus, pleomorphic mitochondria, electron-dense body, Golgi complex, mid-body and endoplasmic reticulum etc. Certain specific structural changes induced by culture filtrates of several mycotoxin-producing fungi have been noted. These alterations induced disappearance of Golgi complex, rER vacuolization, nucleolus attachment to the nuclear envelope nad appearance of certain vacuoles. There were not any changes by the treatment of culture filtrates of non-toxic fungi and only cell debris of some specimens can be observed by the injury of culture filtrates. The experimental animals treated with mycotoxin-producing fungi (Aspergillus flavus ATCC 15517, Aspergillus parasilicus RIB 1037, Penicillum citrinum SWU 238, Penicillium toxicarium RIB 4002, and Penicillium islandicum IFO 5235) were mal cells treated with culture filtrates.
고춘명,김세종,조세훈,김성광,류준,Koh, Choon-Myung,Kim, Se-Jong,Cho, She-Hoon,Kim, Sung-Kwang,Lew, Joon 한국미생물학회 1974 미생물학회지 Vol.12 No.2
With an aim to disclose causal factors scably grains intoxications, the screening of toxic Fusaria and the detection of toxic priniciples were performed in respect of cultured cells bioassay with HeLa cells, skin-necrotizing effect, histopathological investigation and also chromatographic analysis sith following results ; 1. Among the fungi, Fusarium sp. F-27, F-63 and F-61 were highly toxic to mice, causing liver injury characterized necrosis and inflammation. 2. HeLa cell culture bioassay demonstrated that the cell of the isolated strains of Fusaria were suspected to produce toxic material (Fusarenon-X). 3. The culture filtrates of Fusarium nivale Fn-2B, F-27, and F-63, were injected subcutaneously, and caused inflammation followed by crust on the skin ICR-mice. 4. The observation method of skin-necrotizing effect to the mice can be used to the screening to the toxin-producing fungi isolated from many fusarial contaminations.