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Genetic Diversity of Rehmannia glutinosa Genotypes Assessed by Molecular Markers
Kyong-Hwan Bang(방경환),Jong-Wook Chung(정종욱),Young-Chang Kim(김영창),Jei-Wan Lee(이제완),Hong-Sig Kim(김홍식),Dong-Hwi Kim(김동휘) 한국생명과학회 2008 생명과학회지 Vol.18 No.4
RAPD 분석을 이용하여 지황 육성 계통과 지역 수집종 들을 구분할 수 있는 분자표지자를 선발하고, 집단 간, 집단 내 유전적 다양성을 평가하기 위하여 본 실험을 수행하였다. 총 20개의 임의 primer를 이용하여 PCR 한 결과, 육성 계통과 수집종 들을 구별할 수 있는 OPA-1 등 10개의 재현성과 다형성이 좋은 프라이머 들을 선발하였다. 특히 OPA-10, OPA-11 및 OPA-19는 고려지황과 지황1호를 다른 계통 및 수집종 들과 구별할 수 있었으며, 이들 프라이머를 이용하여 0.9 kb, 1.2 kb, 1.3 kb 및 1.4 kb 등의 육성계통 특이적인 DNA 밴드들을 확보할 수 있었다. 한편 이들의 결과를 토대로 통계처리에 의한 유전분석 결과, 고려지황, 지황1호 및 일본지황은 집단 내 유사도가 높아 다른 집단들과 구별되었다. 결론적으로, RAPD 분석을 통한 결과는 지황의 유전적 다양성 이해와 특정 계통을 다른 계통 및 수집종 들과 구분할 수 있는 방법으로 이용될 수 있다. Random amplified polymorphic DNA (RAPD) markers were used to identify the genetic diversities among and within varieties and landraces of Rehmannia glutinosa. Polymorphic and reproducible bands were produced by 10 primers out of total 20 primers used in the experiment. In RAPD analysis of the 11 genotypes, 64 fragments out of 73 amplified genomic DNA fragments were polymorphic which represented an average 6.4 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPA-1) to 13 (OPA-11) and varied in size from 200 bp to 1,400 bp. Especially, OPA-10, OPA-11 and OPA-19 primers showed specific bands for varieties of Korea Jiwhang and Jiwhang il ho, which could be useful for discriminating from other varieties and landraces of R. glutinosa. Percentage polymorphism ranged from a minimum of 50% (OPA-1) to a maximum of 100% (OPA-11), with an average of 87.7%. Similarity coefficients were higher in the genotypes of Korea Jiwhang and Jiwhang il ho than in other populations. In cluster analysis, genotypes of Korea Jiwhang, Jiwhang il ho, and Japanese accession were separated from those of other varieties and landraces. Average of genetic diversity within the population (HS) was 0.110, while average of total genetic diversity (HT) was 0.229. Across all RAPD makers the GST value was 0.517, indicating that about 52% of the total genetic variation could be explained by RAPDs differences while the remaining 48% might be attributable to differences among samples. Consequently, RAPD analysis was useful method to discriminate different populations such as domestic varieties and other landraces. The results of the present study will be used to understand the population and evolutionary genetics of R. gllutinosa.
Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes
Jo, Ick-Hyun,Bang, Kyong-Hwan,Kim, Young-Chang,Lee, Jei-Wan,Seo, A-Yeon,Seong, Bong-Jae,Kim, Hyun-Ho,Kim, Dong-Hwi,Cha, Seon-Woo,Cho, Yong-Gu,Kim, Hong-Sig The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.4
In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes
Ick-Hyun Jo,Kyong Hwan Bang,Young-Chang Kim,Jei-Wan Lee,A-Yeon Seo,Bong-Jae Seong,Hyun-Ho Kim,Dong-Hwi Kim,Seon-Woo Cha,Yong-Gu Cho,Hong-Sig Kim 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.4
In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP II 0, and PGP 130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as "ATA", "GCC", "GTA", "GCA", and" ACC", respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
벤조티아졸기를 갖는 벤조크라운에테르를 전극물질로 사용한 $K^+$ 이온선택성 막전극
홍욱선,권혜경,차근식,남학현,장승현,정광보,Hong, Uk Sun,Kwon, Hye Kyong,Cha, Geun Sig,Nam, Hakhyun,Chang, Seung Hyun,Chung, Kwang Bo 대한화학회 1995 대한화학회지 Vol.39 No.9
33중량%의 PVC와 66중량%의 o-NPOE 가소제에 1중량%의 중성 전극물질인 4'-benzothiazolyl-benzo-15-crown-5를 사용하여 성형한 막전극은 다른 15-크라운-5난 18-크라운-6 유토체들에 비하여 나트륨에 대하여 보다 낮은 선택성을 갖는 칼륨선택성을 나타내었다. 이 막전극의 전기화학적 성질들(감응기울기, 선택성, 섬출한계 및 수명)을 잘 알려져 있는 칼륨선택성 전극물질인 발리노마이신, benzo-15-crown-5, 4'-aminobenzo-15-15-crown-5, benzo-18-crown-6, dibenzo-18-crown-6 및 bis[(benzo-15-crown-5)-4'-ylnethuyl]pimelate를 사용한 전극막들의 것과 동일한 실험 조건하에서 비교하였다. 이러한 비교연구를 통하여 벤조티아졸기가 치환된 벤조크라운에테르 화합물이 증가된 칼륨이온 선택성을 나타내게되는 이유를 치환기의 효과와 이온의 분배계수효과로 설명하였다. The ion-selective membrane fabricated with 33 wt% PVC, 66 wt% o-NPOE and 1 wt% 4'-benzothiazolylbenzo-15-crown-5 (TB15C5) as a neutral carrier exhibited an enhanced selectivity to potassium ion over sodium ion compared to those prepared with other 15-crown-5 or 18-crown-6 derivatives. The potentiometric properties (response slope, selectivity, detection limits and lifetime) of TB15C5-based ISE membranes along with those based on valinomycin, benzo-15-crown-5 (B15C5), 4'-aminobenzo-15-crouwn-5 (AB15C5), benzo-18-crown-6 (B18C6), dibenzo-18-crown-6 (DB18C6) and bis[(benzo-15-crown-5)-4'-ylmethyl]pimelate (PI-Ⅱ) ionophores were carefully examined under the same experimental conditions. The enhanced selectivity of TB15C5-based membrane to potassium is explained in terms of the effect of the benzothiazol functional group and the distribution coefficients of metal ions.