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Barium Carbonate - Matal oxide 혼합물에 의한 휘발성 루테늄 제거
류경옥,김재성 한국화학공학회 1988 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.26 No.4
^(235)U 핵연료 재처리가공이나 핵폐기물 처리시 휘발된 루테늄을 제거하는데 있어서 휘발된 루테늄을 제거할 뿐만 아니라 뒤이어 일어나는 재휘발을 방지하기 위하여 안정한 화합물로 변형시키는 것이 중요하다. 하소온도와 공기유속을 변화시켜 모의루테늄 질산폐액을 증발시키는 동안 하소로 off-gas로부터 발생된 루테늄종(species)의 휘발도를 관측하였다. Fe₂O₃, TiO₂, MnO₂, BacO₃와 BacO₃-Fe₂O₃, BaCO₃-TiO₂, BacO₃-MnO₂ 혼합물 등의 흡착제를 사용하여 기상 루테늄종을 제거하였고 각 흡착제에 대한 제거효율을 측정, 비교하였다. 하소로 off-gas로부터 발생된 휘발하는 루테늄을 제거하기 위하여 사용된 흡착체증에서 BacO₃-Fe₂O₃, BacO₃-TiO₃, BacO₃-MnO₂ 등의 혼합물이 각각 550℃-750℃, 650℃, 550℃에서 휘발하는 루테늄을 효율적으로 제거하였다. 루테늄은 금속산화물과 바륨 카보네이트와의 혼합물과 같은 담체위에 결합되어 RuO₄로의 휘발이 효과적으로 방지될 수 있고 500℃ 이상의 온도에서 안정한 혼합금속 루테네이트를 생성하도록 홉착제와 자발적으로 반응할 수 있는 것으로 생각된다. UV와 IR분석결과 휘발하는 루테늄종은 NO_x의 존재하에서 RuO₄보다는 더 안정한 nitrosyl ruthenium gas complex라고 생각된다. 그러므로 흡착메카니즘은 nitrosyl ruthenium complex가 흡착되고 루테늄 위의 nitric oxide 촉매분해반응에 의해 N₂가 탈착되면서 안정한 상태로 전환하는 것으로 추론된다. In removing the ruthenium volatilized during the ^(235)U nuclear fuel reprocessing and waste treatment, it is important to not only to trap volatilized ruthenium but also to transform it into stable compound to prevent subsequent revolatilization. A calcination temperature and air velocity were varied to observe the volatility of ruthenium species from calciner off-gas during the evaporation of simulated ruthenium nitric acid solution. The adsorbents such as ferric oxide, titanium dioxide, manganese dioxide, barium carbonate and the mixtures of barium carbonate and above metal oxide were used to remove gaseous ruthenium species and also removal efficiencies for each adsorbents were measured. The mixture adsorbents removed effectively volatile ruthenium. It is considered that ruthenium can be effectively preverted from volatilizing as RuO₄ by being bonded to a support such as the mixture of barium carbonate and the metal oxide and that volatile ruthenium species spontaneously can react with the above adsorbents to yield the stable mixed metal ruthenates at temperatures over 500℃. The spectra of UV-visible and IR may be suggested that volatile species is a more stable nitrosyl ruthenium gas complex than RuO₄ in the presence of NO_x. Therefore, the mechanism of adsorption is deduced that the nitrosyl ruthenium complex is adsorbed, then it converts to the stable state, N₂ being desorbed by catalytic decomposition of nitric oxide on ruthenium.
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이경순(Kyong Soon Lee),노재섭(Jai Seup Ro),김현태(Hyun Tae Kim),유재철(Jae Chul Yoo),이승호(Seong Ho Lee) 한국생약학회 1987 생약학회지 Vol.18 No.1
꼬리조팝나무는 장미과(Rosaceae)에 속하는 한국산 Spiraea속식물중 하나이며, 중국에서는 공심류라 칭하고 타박상, 관절통 및 해소거담등에 사용하는 것으로 되어 있다. 저자는 꼬리조팝나무의 식물성분화학적 연구 및 약품자원식물개발의 일환으로 실험에 착수한 바 엽의 열수침액으로부터 황색침상결정인 flavonoid를 검색, 분리하였으며, 뿌리의 MeOH Ext.의 불검화물로부터 무색침상결정을 분리하였다. 엽의 flavonoid인 성분은 TLC, mp, IR, UV, NMR 및 MASS 등의 기기분석을 실시한 결과, 화학 구조 3,5,7,3',4'-pentahydroxy flavone인 quercetin임을 동정하였고, 뿌리의 MeOH Ext의 불검화물에서 얻은 결정은 TLC, IR 및 GLC의 pattern에 의해 β-sitosterol, stigmasterol 및 campesterol의 혼합물임을 동정 확인하였다.
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Structural Characterization of Mouse HAUSP,a Proteolysis Regulator of p53
Hye-Jin Lee,Kyong-Jai Yoo,Kwang-Hyun Baek 한국통합생물학회 2004 Animal cells and systems Vol.8 No.3
The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site-directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.
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Structural Characterization of Mouse HAUSP, a Proteolysis Regulator of p53
Lee, Hye-Jin,Yoo, Kyong-Jai,Baek, Kwang-Hyun The Korean Society for Integrative Biology 2004 Korean journal of biological sciences Vol.8 No.3
The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. mHAUSP cDNA consisted of 3,312 bp encodes 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains. In this study, we carried out site-directed mutagenesis of 6 conserved amino acids (Cys224, Gln231, Asp296, His457, His465, and Asp482) in Cys box, QQD box, and His box. Interestingly, the conserved Gln 231 was not essential for the catalytic activity of mHAUSP. However, the other conserved amino acids were required for deubiquitinating activity of mHAUSP. We performed isopeptidase assay and confirmed that mHAUSP is able to remove ubiquitin from ubiquitinated substrates. In addition, we observed that mHAUSP induces apoptosis in HeLa cells.
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유원재,오갑성,임소영,변재경,문구현,방사익,Yoo, Won-Jae,Oh, Kap-Sung,Lim, So-Young,Pyon, Jai-Kyong,Mun, Goo-Hyun,Bang, Sa-Ik 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.4
Purpose: Tragus is one of the key structure of the normal shape of auricle. We experienced several cases of hypoplastic tragus with preauricular appendage. This article describes the methods of reconstruction of atypical tragus using accessory tragus or macrotragus to make better aesthetic results rather than simple excision. Methods: From April, 2004 to March, 2009, 21 patients got operations by our method. Seven patients had bilateral deformity of tragus. Mean age was 12.7 years. For 17 cases of accessory tragus, simple excision, z-plasty and interpolation flap was performed. For 11 cases of macrotragus, debulking and z-plasty was performed. Mean follow-up period was 9.4 months. Results: Reconstructed tragus looked symmetric with the opposite side in contour, size, direction and partial coverage of auditory meatus. There was no enlargement of remnant appendage for the follow up period and there was no complication such as hematoma, infection and chondritis. Conclusion: In cases of small and deformed tragus, preauricular tissue such as accessory tragus and macrotragus could be a good source of tragal reconstruction.
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양광문,안현경,유근재,송인옥,김진영,송지홍,강인수,전종영,궁미경,최범채 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.4
임상적으로 인지된 임신의 약 15-20%는 대부분 임신 제1삼분기에 자연유산을 초래한다. 유산의 형태 중 어떤 경우는 초음파에서 임신 낭 속에 태아가 보이지 않고 단지 태반의 일부와 배체 외 물질만을 보인다. 이런 상황을 “무태아임신”이라 명명하고 있으며, 그 빈도가 드물지 않음에도 불구하고 초래되는 원인에 대한 이해가 부족한 실정이다. 이에 저자들은 수태산물의 핵형 분석을 통하여 무태아임신의 발생과의 연관성을 알아보기 위해 본 연구를 시행하였다. 연구대상은 1994년 1월 1일부터 1997년 12월 27일까지 삼성제일병원 습관성 유산 클리닉에 등록된 환자를 대상으로 계류유산을 진단 받고 소파수술을 시행 받은 환자 중 무태아임신 28예와 자궁 내에서 확인된 태아임신 115예를 구분하여 염색체 검사를 시행하였으며, 본 연구에 참여한 환자부부는 모두 정상 핵형을 가지고 있었다. 소파 수술 후 수태산물에서 얻은 융모막 세포는 표준 G-분염법을 사용하였으며 핵형분석을 위해 장기배양을 시행하였다. 환자의 연령이나 유산빈도, 원인에서는 두 군간의 차이가 없었다. 무태아 임신 유산에서는 염색체검사상 45% (52/115)가 정상 소견을 보였으며 자궁 내에서 태아가 확인되었던 유산에서는 46% (13/28)에서 정상소견을 보였고 비정상 염색체의 빈도에 있어서도 두 군간에 차이점이 없었다 (p>0.05, Chi-square test). 또한 염색체 이상소견 중 이수성 (aneuploidy)과 다배수성 (polyploidy)의 빈도도 비슷하였고 또한 배수성 (euploidy) 자연유산의 빈도에서 성별의 차이점도 발견되지 않았다. 결론적으로 무태아 임신과 자궁 내 태아임신의 핵형 이상의 발생빈도에서 차이점이 없으며 임신 낭 내에 존재하는 수태산물의 형태는 염색체 이상유무와 무관하다고 생각된다. 무태아임신의 형성에 관한 새로운 원인 규명을 위해서는 우선 태반과 자궁탈락막사이의 면역학적인 역할을 규명해야 하며 자궁 내 존재하는 태아에 미치는 연관성을 조사해 보는 연구가 흥미로울 것으로 사료된다. Objectives : Approximately 15-20% of all clinically recognized pregnancies result in spontaneous abortion between 8 and 12 weeks. In some early abortus only the extra-embryonic components of the conceptus survive, either as fragments of placenta or as empty gestational sac, and these have been termed ‘anembryonic pregnancies'. Despite their common occurrences, the etiology of anembryonic conceptuses is poorly understood. The purpose of this study was to evaluate whether chromosomal abnormalities relate to a role in anembryonic pregnancy. Materials and Methods : The study, that consisted of 143 pregnancies from chromosomally normal couples that aborted in first trimester period between January 1, 1994 and December 27, 1997, had chromosomal analysis performed on the products of conception and had ultrasonographic examination prior to spontaneous abortion. The tissue was processed and analysed using standard G-banding cytogenetic techniques with long-term cultures. Results : The patient characteristics of the anembryonic pregnancy were not significantly different from those of the embryonic pregnancy. Among products of conception from embryonic pregnancies, 45% (52/115) had normal chromosomal analyses and 55% (63/115) had abnormal chromosomal analyses. Products of conception from anembryonic pregnancies resulted in 54% (15/28) of abnormal chromosome analyses and normal chromosomal analyses resulted in 46% (13/28) (p>0.05, Chi-square test). The frequency of aneuploidy and polyploidy were not different between products of conception from anembryonic pregnancy and embryonic pregnancy. Also, the gender ratio of euploid spontaneous pregnancy losses showed similar in this study. Conclusion : There are no differences in frequency of abnormal karyotypes in products of conception from anembryonic pregnancies compared with miscarriage after the demonstration of fetal pole. If further studies are need for the evaluation of these etiologies, and then immuno-molecular studies in early placental tissues from anembryonic pregnancy might be helpful.
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유원재,임소영,변재경,문구현,방사익,오갑성,Yoo, Won-Jae,Lim, So-Young,Pyon, Jai-Kyong,Mun, Goo-Hyun,Bang, Sa-Ik,Oh, Kap-Sung Korean Society of Plastic and Reconstructive Surge 2010 Archives of Plastic Surgery Vol.37 No.5
Purpose: Full thickness skin grafts are useful in the reconstruction of facial skin defects when primary closure is not feasible. Although the supraclavicular area has been considered as the choice of donor site for large facial skin defect, many patients are reluctant to get a neck scar and some patients do not have enough skin to cover the defect owing to the same insult occurred to the neck such as burn accident. We present several cases of reconstruction of facial skin defects by freehand full-thickness skin graft from anterolateral chest wall resulting aesthetically acceptable outcome with lesser donor site morbidity. Methods: Retrospective review was performed from March, 2007 to September, 2009. 15 patients were treated by this method. Mean age was 31.5 years. The ethiology was congenital melanocytic nevus in 7 cases, capillary malformation in 5 cases and burn scar contracture in 3 cases. Mean area of lesion was measured to 67.3 cm2 preoperatively. The lesion was removed beneath the subcutaneous fatty tissue layer. The graft was not trimmed to be thin except defatting procedure. For the larger size of defect, two pieces of grafts were harvested from both anterolateral chest wall in separation and combined by suture. Results: The mean follow up period was 9.7 months. All the grafts survived without any problem except small necrotic areas in 4 cases, which healed spontaneously under conventional dressings in 6 weeks postoperatively. Color match was relatively excellent. There were 2 cases of hyperpigmentation immediately, but all of them disappeared in a few months. Conclusion: In cases of large facial skin defects, the anterolateral chest wall may be a good alternative choice of full-thickness skin graft.
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