구강양치액에 따른 구강환경지수 및 구강미생물균수의 변화에 관한 연구 : 구강양치액에 따른 구강 환경지수, VIC 및 구강미생물군수의 변화에 관한 연구

이은숙 ; 김혜진 김천대학교 2004 김천대학교 논문집 Vol.25 No.-

This study is to investigate the residents with the symptoms of gin-givitis and periodontitis who agreed on the participation of experimental process among them who visited the laboratory in the department of dental hygienists. K college, in order to diagnose the dental prophylaxis, preventive treatment. They were divided into Distilled water group. Chlorhexidine group. and Leaf Extract group. And, this study is to investigate the change of number of bacteria in the oral cavity according to the status of dental environment, PHP, VSC, and G.I. using the mouth rinse in the same condition for 3 days. 1. As a result of dental examination of experimental object, the mea suring value of dmft was average 5.83, and the dt was 5.39, and VSC was 84.96ppb. PHP was 6.31, and the gingival bleeding index was 57.03%, and the gingival index was 2.16. and the periodontal pocket was 3.51mm. 2. As a result of examining the change of PHP and VSC using the mouth rinse by the group for the experimental object for 3 days, PHP significantly increased to 6.86 in the first day of visiting, 7.19 in one day later, 8.03 in two days later. 8.31 in the final day when it uses the dis-tilled water (p<0.01) The chlorhexidine and leaf extract group rapidly increased after the first day, and then it found that it increased smooth-ly after the second night. In the group using the DW, the VSC signifi-cantly increased from 89.71 ppb in the first day to 154.29ppb on the third day. (p<0.01) Also, the leaf extract group significantly increased from 88.56ppb to 121.89ppb(p<0.1), and VSC had an effect on the inhibition of the bacteria growth by the chlorhexidine. 3. As a result of examining CFU according to the depth of periodontal pocket, it found that the leaf extract group(6.07x104) and chlorhexi-dine (4.22 104) had an effect on the inhibition of the bacteria growth more than the DW (29.15 x 104) in a group with the below 3.5mm in the depth of periodontal pocket. As for the CFU in the salvia and gingival sulcus, the number of bacteria after using the mouth rinse in the whole three groups increased too highly in the salvia sampled using Peptone gargle. As for the change of number of bacteria after Paper point pack-ing. the group of distilled water increased significantly. But, it found that the group using the chlorhexidine and the leaf extract increased smoothly. 4. As for CFU of G.I before the treatment of dental prophylaxis, the higher the gingival dimension was according to the sorts of mouthrins-ing, the more the number of bacteria(p<0.01) was. After the use of mouth rinse, it found that the higher G.I was, the more CFU was. In the chlorhexidine and the green tea extract, the higher G.I was, the less CFU was or the more it increased smoothly. After all, it found that the higher G.I was, the more it had an effect on the use of mouthrinse.

유치원 자모의 구강보건인식도에 관한 연구

이은숙 ; 강경희 ; 김혜진 김천대학교 2002 김천대학교 논문집 Vol.23 No.-

The purpose of this study are to do surveys of the attitude and behavior about oral health of mothers with infants and to apply those results to the systematic and efficient oral health care.

한국산 관박쥐(Rhinolophus ferrumequinum korai) 설유두의 미세구조 관찰

이정훈,손성원,이혜진 慶南大學校 附設 基礎科學硏究所 2000 硏究論文集 Vol.14 No.-

한국산 관박쥐(Rhinolophus ferrumequinum korai) 설유두의 형태적 특지을 알아보기 위하여 광학 및 전자현미경으로 조사한 결과는 다음과 같았다. 관박쥐의 설유두는 사상유두(filiform papillae), 융상유두(fungiform papillae), 유곽유두(vallate papillae) 그리고 엽상유두(foliate papillae)가 분포하고 있었다. 사상유두는 전단부에서는 5-10정도의 침상돌기를 가지고 있으며, 중앙부위(중단부)의 사상유두는 침상돌기를 거의 갖지는 않지만 매우 날카로운 가시모양을 하고 있었고, 후단부는 대형의 사상유두가 존재하고 있었다. 후단부에는 2개의 유곽유두가 존재하고 있었으며, 또한 혀의 전단부와 중단부에 걸쳐 사상유두 사이에는 융상유두가 존재하고 있었다. 혀의 기부 측면에는 잎새모양의 엽상유두들이 존재하고 있었으며, 융상, 유곽 및 엽상유두내에는 다수의 미뢰가 관찰되었다. To investigate the morphological features of the lingual papillae of Rhinolophus ferrumequinum korai was examined by light microscope and scanning electron microscope. The results were as follows. The tongue of R. ferrumequinum korai papillae. Each filiform papillae has about five to ten of the needle-shaped protrusion. Filiform papillae in the central region of tongue has a very sharpe protrusion. The large of filiform papillae were observed in the posterior region of tongue, and two vallate papillae were only located on the posterior region of the tongue. The fungiform papillae were distributed among filiform papillae at the anterior and middle region of tongue. Foliate papillae were located on the anterior region of lateral view of the tongue. Numerous taste buds were observed in the epithelium of fungiform, vallate, and foliate papillae.

이주를 경험한 남녀의 서사를 통해 본 친밀한 관계성의 변화

이혜진 이화여자대학교 한국여성연구소 : 이화여자대학교 한국여성연구원 2013 여성학논집 Vol.30 No.2

This study investigates how migration experiences have affected to gender consciousness and intimate relationship between South Koreans in Japan Migrant workers have experienced the change of intimate relationship with their cross-border move(migration). In particular, they have given the meaning of the place as not the foreign one but making relationship as their daily lives through a long-term life in the foreign country. The status of ‘illegal stay’ acts as an element which makes difficult coming and going to their hometowns and family so that they have experienced the change of intimacy in the spiral of the relationship unconsciously or consciously. It is the intimate relation area where is overlapped of sexuality, gender consciousness and a family norm that remarkably appear realistic society, ritual and conflict between their body and mind. Therefore, focusing on the relations between migration experience and intimacy, this research reveals that the relations between ‘illegal stay’ status and intimate relationship are formulated within the limited social network, lead to work opportunity and income stability in itself and function as mechanism of maintaining their life as migrants. 본 연구는 이주 경험자의 젠더의식 및 친밀한 관계성에 이주 경험이 어떤 변화를 발생시켰는지를 고찰하고자 한다. 국경을 넘는 이동을 계기로 이주자들은 친밀한 관계성의 변용을 경험하게 되는데, 특히 장기간에 걸친 타향(他鄕)에서의 생활은 그곳이 이미 타향이 아니라, 자신의 일상의 현실로서 새로운 관계성을 형성하게 된다. 이주자들은 의식적이든, 무의식적이든 그 관계성에 휘말려, 어쩔 수 없이 자신의 친밀권(親密圈)의 변용을 경험하게 된다. 또한, 섹슈얼리티와 젠더의식, 가족규범이 중첩하는 친밀한 관계성의 영역을 분석하여 자신이 실제로 존재하고 있는 현실 사회와 그들의 삶을 관장하는 의식 및 몸의 경합이 이주자들의 삶에서 어떻게 드러나고 있는지 살펴본다. 따라서 본 연구는 이주경험과 친밀한 관계성의 관계에 주목하여, 이주자가 가진‘불법’적 지위와 친밀한 관계성의 성격은, 그들이 가질 수 있는 극히 제한된 사회적 연결망 속에서 형성되고, 친밀한 관계성 자체가 일자리 및 소득의 확보와 연결되며, 이주자로서의 전면적인 삶을 유지할 수 있는 기제로 작용하고 있음을 밝혔다.

김천대학생들의 구취인지 실태에 관한 조사연구

이은숙 ; 김혜진 ; 김희천 ; 최옥선 김천대학교 2003 김천대학교 논문집 Vol.24 No.-

To help the guidance of treatment method, prevention of halitosis, and oral hygiene management for halitosis patient or non-halitosis group for investigating the status in accordance with awareness of halitosis to improve the oral health for college students, the survey including ques- tionnaire was performed for 331 persons of students who is studying in the Gimcheon college located in the northern province of Kyeongsang-Do. As a conclusion, the following data are obtained. 1. For the question of "I am acknowledging the halitosis", the replying person were 123 persons (37.2%), including men-69 persons(20.8%), women-54 persons(16.3%), So, in case of the ratio of acknowledging the halitosis, men was higher than women. On the contrary, for the question of "I do not acknowledging the halitosis", men-68 persons (20.5%), women-140 persons(42.3%), So, in case of the ratio of non-ackowledging the halitosis, men was much higher than women. 2. For the question of "When did you acknowledge the halitosis at most in the whole day?", total of 127 persons(38.4%) said "as soon as get up" out of the 183 persons (55%) who acknowledging the halitosis. The remaining replies were followed in the order of "before breakfast", "after breakfast", and "before sleeping". 3. In the status of oral hygiene, it showed that the persons who acknowledge the halitosis acts positively the frequency of toothbrushing (more than twice a day), method of toothbrushing, and brushing the tongue than the persons who do not acknowledge the halitosis. 4. In the status of smoking habits, there was no increment according to the duration of smoking, but in the status of amount of smoking, the persons who smokes less than half carton a day were 20 (6.0%), half through one carton a day were 31 (9.4%). So, it showed that the amount of smoking is affected to the cause of halitosis. 5. The ordinary prevention methods for oral hygiene according to the awareness of halitosis were investigated in the order of toothbrushing/brushing the tongue (37.3%), chewing the gum (33%), and using the rinsing liquids (12.4%).

동면주기에 따른 관박쥐(Rhinolophus ferrumequinum korai) 위장관 점액세포의 조직화학적 연구

손성원,이혜진 慶南大學校 附設 基礎科學硏究所 2002 硏究論文集 Vol.16 No.-

이 연구는 활동기 동면전기, 동면기, 동면후기에 따른 박쥐의 위장관 점액세포에 대한 조직화학적 변화를 밝히고자 실행되었다, 점액성상을 알아보기 위해서 중성점액질은 periodic acid schiff (PAS) 염색법, 산성점액질은 alcian blue pH 2.5 (AB 2.5) 염색법, 중성점액질과 산성점액질의 조성을 검색하기 위해 AB 2.5-PAS 염색법을 실시하였다. 그 결과 활동기의 위의 경우 강한 PAS양성과 강한 AB 2.5 반응을 나타내었으며, 소장의 배상의 경우 PAS 반응과 AB 2.5 염색에서 강한 염색성을 보였다. 동면기때 PAS반응도는 약하게 나타났으며, AB 2.5의 경우도 점액 함량 및 반응도가 현저히 떨어짐을 볼 수 있었다. 배상세포는 PAS와 AB 2.5 염색의 경우 전체적으로 중등도염색성을 보였다. This study was performed to clarify the histochmical changes of mucus secreting cells in the gastrointestinal mucosa of bats in the active, prehibernating, hibernating, and posthibernating phases. The mucosubstance was observed with periodic acid-Schiff (PAS), alcan blue pH2.5(AB 2.5), AB 2.5-PAS seqential staining reactions differentiated acidic mucin from neutral mucin. The results were as follows. In active phase, suface mucous cells and mucous neck cells of the stomach show from moderate to strong postivity PAS reaction and strong to moderate postivity AB 2.5 rection. Goblet cells show from marked postivity PAS reaction and moderate postivity AB 2.5 rection. In the hibernating phase, moderate postivity PAS reaction and trace postivity AB 2.5 reaction. Goblet cells show from moderate postivity PAS reaction and moderate postivity AB 2.5 rection.

Genetic diversity and population structure of the Black-faced Spoonbill (<i>Platalea minor</i>) among its breeding sites in South Korea: Implication for conservation

Lee, Mu-Yeong,Kwon, In-Ki,Lee, Kisup,Choi, Sung Kyoung,Jeon, Hey Sook,Lee, Ji-Yeon,Eo, Kyung-yeon,Kim, Hwa-Jung,Kim, Jin-Han,Johnson, Warren E.,Yoo, Jeong-Chil,An, Junghwa Elsevier 2017 Biochemical systematics and ecology Vol.71 No.-

<P><B>Abstract</B></P> <P>The endangered Black-faced Spoonbill <I>Platalea minor</I> has experienced drastic reductions in population size, geographic distribution, and habitat availability throughout East Asia. In the present study, we examined population genetic structure and genetic diversity of Black-faced Spoonbills inhabiting five sites off the west coast of South Korea encompassing a few of its major breeding sites. Ten microsatellite loci and the mitochondrial sequence were used to assess patterns of genetic variation based on 63 individuals. Three ND2 haplotypes were found among 61 individuals; the remaining two were identified as Eurasian Spoonbills, revealing an unexpected hybridization between these two species having different ecological niches in South Korea—the Eurasian Spoonbill overwinters in inland areas, whereas the Black-faced Spoonbill inhabits coastal areas during the summer. Analyses of microsatellite variation revealed no discrete population structure among the five breeding sites but very weak genetic differentiation among geographically distant regions. Assignment tests identified several possible migrants among sites. Our findings suggested that Black-faced Spoonbills from the five breeding sites could be managed as a single population and highlighted the importance of conserving the populations from Maedo, Suhaam, and Namdong reservoir, which are geographically close and have retained high levels of genetic diversity and large populations.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ten polymorphic microsatellite loci and the mitochondrial DNA sequence were used to assess genetic diversity and structure. </LI> <LI> No discrete population structure among the five breeding sites in South Korea but very weak genetic differentiation among geographically distant regions. </LI> <LI> Black-faced Spoonbill individuals from the five breeding sites could be managed as a single population in South Korea. </LI> <LI> Unexpected hybridization between the Eurasian Spoonbill and the Black-faced Spoonbill was observed in our genetic studies. </LI> </UL> </P>

Passaging Method for Expansion of Undifferentiated Human Embryonic Stem Cells by Pipetting Technique

Lee, Sung-Geum,Moon, Sung-Hwan,Lee, Soo-Hong,Lee, Hey-Jin,Kim, Jae-Hwan,Chung, Hyung-Min The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4

We have developed a new passaging technique for the expansion of human embryonic stem cells (hESCs) that involves simply pipetting portions of hESCs acquired from colonies, reducing the laborious and time-consuming steps in the expansion of hESCs. Compared to general mechanical methods of passaging, our pipetting method allowed hESCs colonies to be broken into small fragments, which showed significantly higher attachment rates onto feeder cell layers. This technique produced three times the number of hESCs colonies than conventional mechanical methods. In addition, this pipetting method allowed us to distinguish differentiated hESCs from undifferentiated hESCs during hESCs colony pipetting. The hESCs cultured by pipetting method displayed normal human chromosomes for over 60 passages. According to RT-PCR and immunohistochemical analysis, the hESCs successfully maintained their undifferentiated state and pluripotency which was also confirmed by teratoma formation in viva Therefore, the pipetting method described in this study is a useful tool to efficiently and quickly expand hESCs on a large scale without enzyme treatment.

Acupuncture Treatment about Medial Meniscus Posterior Horn Rupture : A Case Report

Hey-Jin Lee,Nam-Heon Lee,Chang-Gue Son 大田大學校 附屬 韓方病院 2020 惠和醫學 Vol.29 No.2

Obectives: In this case, the knee joint inconvenience with deteriorating pain has been relieved by acupuncture treatment for a patient was 51-year-old male with a left medial meniscal posterior horn rupture in 2012. Methods : Twenty-four times of acupuncture treatments were performed for Twelve weeks from July 2016 to improve the disease. SP8(Jigi; 地機), BL63(Geummun; 金門), KI3(Taegye; 太谿), LI4(Hapgok; 合谷) were chosen for treatment by principles of Traditional Korean Medicine. Results : Numeric Rating Scales, which means subjective pain, decreased from 6 points to 3 points out of 10 points in total. Pressure Pain Threshold, which means sensitivity to pressure applied to the affected area, increased from 21N to 47N on the lateral-inferior side and from 19N to 50N on the lateral side. K-WOMAC, which indicates discomfort of knee-related activity, was 56 points out of 96 points in total before treatment and 4 points after treatment, 13 points after two years, and 15 points after four years. However, MRI tests conducted before and after treatment did not identify any significant changes.As a result, we confirmed that a total of 24 acupuncture treatments had resulted in the relief objective and subjective pain and functional recovery, especially in the case of the knee function, maintained until after four years later. There was no significant substrate recovery in meniscal repture.

Poster Session : Others ; Antiviral effect of curcuma Longa Linn extract against Hepatitis B virus replication

( Hey Jin Kim ),( Hwa Seung Yoo ),( Sang Yong Kim ),( Chan Su Park ),( Jin Chul Kim ),( Soo Yeon Lee ),( Jong Min Choi ),( Jeong Keun Ahn ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-