Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

Bicycle Ergometer를 통한 고교남자 태권도·필드하키선수의 Cardiorespiratory 및 Cardiocirculatory 운동부하능력 비교 연구

김진홍,최경훈 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.2

본 연구는 실내에서 주로 이루어지는 태권도와 실외 운동인 필드하키 남자 고교선수의 운동능력을 운동생리학적으로 분석하여 스포츠종목에 따른 운동능력의 차이와 한계가 어느 정도인지를 명확하게 구분하는데 목적이 있다. 연령과 성별에 따른 다른 스포츠 종목의 운동생리학적 스포츠의학적 운동부하능력 구분은 이미 60년대부터 있었으나 태권도를 실외 스포츠와 비교한 연구는 전무하고, 태권도가 건강을 증진에 기여하는 스포츠 종목으로 정립하고, 태권도와 필드하키가 가지는 운동 생리학적 특성을 분석하여 상호 미진한 부분을 보완하는 계기를 마련하는 자료를 도출하는데 두 번째 목적이 있다. 본 연구에 참여한 피검자로는 부산지역의 K고교 1,2학년 태권도 선수와 경남 K고교 필드하키 선수 1, 2학년을 종목별 8명을 대상으로 선정하였다. 인제대학교 스포츠의학실험실의 검진도구(서독의 Jaeger사의 Alpha Oxycon과 네덜란드산 Lode의 Bicycle Ergometer)를 이용하여 매 2분마다 1Watt/㎏ BW의 점진적인 운동부하의 증가방법인 NOWACKI의 Protocol에 따라 운동검진에 참여한 선수가 주관적 또는 객관적으로 탈진상태(all out)에 이를 때까지 심장순환계(HRmin^-1), 최대산소섭취량(V˚O_2max.), 상대적 최대산소섭취량(V˚O_2max/㎏.BW), 1회 심장의 박동에 따른 산소의 섭취량(O_2/HR), 산소섭취량에 따른 이산화탄소 배출량(RQ)과 분당 호흡수(BFmin^-1)를 운동시 2분, 4분, 6분, 8분, 최대운동, 운동 후 휴식기 1~5분을 관찰하였다. 검진에서 얻어진 모든 데이터는 Windows용 SPSS 통계프로그램을 이용하여 분석하고 태권도 선수와 필드하키 선수를 변인들간의 차이를 기준으로 t-test로 유의검증을 하고 결과를 해석하였다. 연구의 검진에서 얻어진 데이터의 분석결과 태권도 선수보다 필드하키 선수가 각 변인들 심장순환계(HR), 최대 산소 섭취량(V˚O_2max), 상대적 최대산소섭취량(V˚O_2max/㎏.BW), 1회 심장의 박동에 따른 산소의 섭취량(O_2/HR)등의 운동부하능력에 있어서 좋은 결과를 보일 것으로 예상했으나 평균값에서 태권도 선수가 높게 나타났다. 그래서 원인이 될 근거를 탐색한 결과 첫째, 태권도 선수의 운동경력이 필드 하키선수보다 더 많았고, 둘재, 실험방법에 있어 자전거 에르고미터(Bicycle Ergometer)의 이용이 필드하키 선수보다 태권도 선수에게 더 유리하게 작용했으며, 셋째, 태권도선수가 하키선수보다 신장과 체중이 크고 많이 나가는 것으로 나타났다. 즉 태권도 선수의 경우 고정된 자세에서 하지의 근력을 많이 사용하는 반면에 필드하키선수는 동적인 자세에서 다리의 근력이 사용되는 이유로 앉아서 행하는 자전거 에르고미터(Bicycle Ergometer)에 필드하키 선수의 적응이 부정적으로 작용한 것으로 여겨진다. 그래서 본 연구에서 얻어진 결과로 다음과 같은 제안을 하고자한다. 즉 스포츠 종목의 특성에 따라 나타난 운동 생리학적 결과로 평소 스포츠 종목의 특징에 따른 국한된 트레이닝보다 다양한 프로그램의 적용을 통한 훈련 이 이루어져야 할 것이다.

문제행동 청소년을 위한 대인관계 집단치료 효과

이후경,안현주,김선재,윤성철,봉수연 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.5

Objectives : Fast change and confusion of the value system in society affect the identity formation of adolescents. So Adlescents' behavioral problems seem to be increasing yearly. It is known that the interpersonal group therapy is the most effective treatment modality among many group programs for adolescents with behavioral problems. Objectives of this study are as follows ; first, evaluate therapeutic effects of the interpersonal group therapy for adolescents with behavioral problems second, examine the therapeutic factors ; third, analyze the group process : fourth, orgamize the group therapy for adolescents with behavioral problems as an applicable treatment modality. The types of group therapy performed in this study are small, closed, homogeneous, outpatient, timelimited, and adolescent group. Methods : This study was carried out at one of the adolescents mental health services in Uiwang Mental Health Center, from April to June and from September to November in 2000. The subjects were 2nd grade students in K middle school with behavioral proplems. The experimental groups were composed of 4 groups including 2 male groups and 2 female groups (male 11, female 17), and the control groups matched the same conditions as the experimental groups (male 10, female 19). The group therapy was performed weekly in CA (club activity) time, and it took 60-70 minutes at one time. They met total 10 sessions including preparatory meeting and termination meeting. Before and after this program, self-rating scales (behavioral problems, aggression, and impulsivity) were applied for subjects, and the teacher rating scales (behavioral problems, aggression and impulsivity) were performed by teachers in charge. After each session, Yalom's 13 therapeutic factors scale was perfbrmed. At the last meeting, total assessment questionnaire was compleled. Results : The mean scores of all self-rating scales (behavioral problem, aggression, and impulsivity) were lower in the experimental groups (both male and female group) than the control groups, but not significant statistically except the behavioral problem scale of female students. The mean scores of all teacher rating scales (behavioral problem, aggression, and impulsivity) were lower in the experimental groups (both male and female group) in comparison with the control groups, all significant statistically. As faras the therapeutic factors are concerned ; first, the mean scores of all therapeutic factors in female students were higher than in male students and the mean scores of all therapeutic factors in the later half sessions were higher than the former half sessions ; second, catharsis and existential factor were high in all students and sessions ; third, identification with therapist factor was high in all students and sessions : fourth, during the later half sessions, interpersonal input was relatively high in male students and socializing technique was relatively high in female students. Its forthe total assessment questionnaires ; 100% of male students and 88.2% of female students reported that they were helped by this group therapy ; 100% of male students and 82.4% of female students reported that they would participate willingly if future opportunity of the same group therapy is given for them. Conclusion : Adolescents with behavioral problems who participated in this study showed the decrease of behavioral problems and the change of aggressive and impulsive attitudes in comparison with the control groups although they had some differences between male and female students. The group therapy with adolescents would be practiced more broadly and extensively though there are several accompanied problems including the difficulty of structuring, the deficit of motivation, and financial problem.

예도(銳刀)의 미시사적 관점으로 바라본 무예실제에 관한 분석

송일훈,이태현,장성호,장경태,김대양 龍仁大學校 武道硏究所 2011 武道硏究所誌 Vol.22 No.1

예도(銳刀)에 관한 보다 정확한 고찰은 오늘날 전통무예단체들이 『武藝圖譜通志』를 통한 전통무예의 실제를 복원 및 재현함에 있어 크나큰 도움이 될 것이다. 이는 예도(銳刀)가 전통무예사에 대한 단초적 역할을 제공할 수 있는 초석이 될 수 있기 때문이다. 따라서 이 연구에서는 예도의 설(說: 해설(解說))과 기(技: 기예(技藝))의 형식적 분석과 전통무예의 복원에 관한 이론적 배경을 정립하기 위하여 고서와 도록 등의 다양한 자료를 검토‧분석하였는데 이는 다음과 같은 결론을 도출하였다. 첫째, 예도(銳刀)의 종합적 분석을 통한 예도의 형식은 병기도식(兵器圖式)․설(設) 11개․예도보(銳刀譜)․예도총보(銳刀總譜)와 예도총도(銳刀總圖)로 구성되어 있음을 알았다. 둘째, 예도는 본국검과 더불어 조선을 대표하는 한국 고유의 전통검법으로 임진왜란을 계기로 새롭게 발굴 정립되었으며 정조가 재위했던 1800년대까지 약 200년 걸쳐 전승되면서 민족의 재산과 생명을 지켜왔던 국방무예(24반 무예)였다. 셋째, 국난을 극복하고 국토를 수호하려는 선조들의 의지로 완성된 24반 무예 속에 녹아 든 활달한 몸짓 속에는 민족자존의 의지와 불굴에 투혼이 배어 있다. 이와 같이 오늘날 한․중․일의 칼 쓰는 법을 비교해 볼 때, 『武藝圖譜通志』의 예도가 갖는 의미와 가치는 대단히 귀중하다 할 수 있겠다. 이상과 같이 종합해 볼 때, 앞으로 이 한국무예 예도(銳刀)에 관한 심도 있는 연구가 이루어질 때 비로소 예도(銳刀)가 세계적인 보편무예로 발전하는 중요한 기반이 될 것으로 기대하는 바이다.

Spiroxazine유도체의 분자 광스위칭을 이용한 능동형 평판 광도파로 자외선센서

옥경식,김시홍,김성훈,김응수,고광락,강신원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

UV Sensor is widely used for various fields with the study on UV detector and UV related researches. Also due to the recent increasing of UV radiation by ozone depletion enforces the development of techniques on efficient UV detection. Thus, in our study, we proposed novel UV sensor using photochromic molecular device that has good UV sensitivity and easiness to manufacturing, and low cost. Furthermore, this molecular device is useful for the construction of a thin film active waveguide type device that has excellent sensitivity and feasibility to miniaturization.

강진만 해역의 다모류 군집의 종조성 및 계절변동

윤성민,김춘철,서원일,황선영,이성훈,한경호,이우범 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

To detemine seasonal fluctuations in abundance and speices composition, polychaete samples were collected by Van Veen grab form March to November 2003 in Kangjin Bay. A total of 25,119.3 ind./㎡ polycaete were sampled and identified into 48 species. 25 families and 10 orders. Of the 10 orders. Phllodocida, Spionida, Eunicida and Sabellida accounted for approximately 79.9% of the polychaete fauna in this area. Phyllodocida, Nereidae, Nephtyidae ans Spionidae were dominant representing every moment 4 species. Of 48 species identified Neanthes japonica, Prionospio pinnate, Cirratulus cirratus and Palola siciliensis for 54.0% individuals collected. Season succession of dominant species was evident in study area: Neanthes japonica, Prionospio pinnata. Cirratulus cirratus. Nephtys oligobranchia in March, Neanthes japonica, Lumbrineris japonica. Cirratulus cirratus, Prionospio pinnata in May, Ncanthes japonica, Cirratulus cirratus, Lumbrineris nippnica. Lumbrineris japonica in August and Neanthes japonica, Prionospio pinnata, Laonice cirrata, Palola siciliensis in November, respectively. The diversity index was the highest value in March(H'=3.0059) and the lowest value in May(H'=2.7305). The eveness index was the highest in August(J=0.9184) and lowest in May(J=0.8524). The dominance index was the highest in May(D=0.3843) and the lowest in March(D=0.2275).

Appropriate feeding for early juvenile stages of eunicid polychaete Marphysa sanguinea

( Kyeong Hun Kim ),( Byoung Kwon Kim ),( Sung Kyun Kim ),( War War Phoo ),( B. A. Venmathi Maran ),( Chang-hoon Kim ) 한국수산과학회(구 한국수산학회) 2017 Fisheries and Aquatic Sciences Vol.20 No.3

Survival rate (SR) and growth rate (GR) were tested with various feed sources to identify an appropriate feed to improve the productivity in the early life stage of the rock worm Marphysa sanguinea (Montagu, 1813) (Eunicidae: Polychaeta). In addition, feed supply rates were also examined. Three experiments were performed to identify the appropriate feed for the juvenile stages of M. sanguinea. Experiment 1 was done using seven different feed sources and without feed as well for the first 20 days of M. sanguinea culture. Decapsulated Artemia and extruded pellet for shrimp were showed with high SR and GR in the experiment 1. Experiment 2 was performed with five different feed sources. Two feeds were selected from experiment 1 in addition to eel feed, mixed micro-algae, and benthic diatom. Four different quantities of each feed were supplied to 3000 individuals of early juvenile stage of M. sanguinea. High quantity of decapsulated Artemia and shrimp feed resulted with a relatively good SR and GR. In experiment 3, we provided 20, 50, and 75 mg of decapsulated Artemia and shrimp feed to 3000 individuals of M. sanguinea. Our results demonstrated that after 3 months, decapsulated Artemia showed high survival rate and 75 mg/3000 inds provided the best quantity of feed in the earlier life stage culture of M. sanguinea.

REDUCTION OF DISCHARGED SLUDGE FROM LAND-BASED AQUACULTURE FARMS BY THE INTEGRATED CULTURE OF ROCKWORM Marphysa sanguinea

Kyeong-Hun Kim,Sung-Kyun Kim,Byung-Kwon Kim,Jae-Hoon Cha,Jae-Keun Lee,Chang-Hoon Kim 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

총설 : 수직유해물질 배출허용기준 설정에 따른 배출시설 비용영향 분설사례 연구: 1,4-Dichlorobenzene을 중심으로

김경진 ( Kyeong Jin Kim ),김원기 ( Won Gi Kim ),허진 ( Jin Heo ),김광인 ( Kwang In Kim ),김재훈 ( Jae Hoon Kim ),김상훈 ( Sang Hun Kim ),염의태 ( Ick Tae Yeom ) 한국물환경학회 ( 구 한국수질보전학회 ) 2010 한국물환경학회지 Vol.26 No.4

총설 : 처리기술에 근거한 산업폐수 배출허용기준 국내 적용성 연구(2): 산업폐수 위해성 지표를 이용한 오염부하 기여도 분석

김경진 ( Kyeong Jin Kim ),김원기 ( Won Gi Kim ),정상구 ( Sang Gu Jung ),정진영 ( Jin Young Jung ),김재훈 ( Jae Hun Kim ),김상훈 ( Sang Hun Kim ),염익태 ( Ick Tae Yeom ) 한국물환경학회 ( 구 한국수질보전학회 ) 2010 한국물환경학회지 Vol.26 No.2