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( Kenji Furukawa ),( Pham Khac Lieu ),( Hiroyuki Tokitoh ),( Ritsuko Hatozaki ),( Takao Fujii ) 한국물환경학회 ( 구 한국수질보전학회 ) 2005 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2005 No.-
Cultivation of extremely slow growing anammox bacteria was succeeded using up-flow column reactor packed with novel polyester non-woven as biomass carrier. Characterization of an anammox community on a polyester nonwoven attachment medium revealed an organism with a 16S rDNA sequence similarity of 92.2% to the archetypal Candidatus B. anammoxidans. The newly discovered strain (KSU-1, AB057453) was dominant (72.8%) among detectable members of a biofilm community. By fluorescence imaging, KSU-1 was shown to form spherical granular clusters wrapped in a thin layer of Zoogloea sp. This type of anammox reactor was successfully scale-up to the working volume to 50 L. Maximum total volumetric nitrogen removal rate of 1.68kg-N/㎥/d was achieved for the 4 months continuous operation. For the application of anammox reaction to the nitrogen removal process, half of influent NH4-N must be converted to NO2-N as a preceding step for anammox reaction. Operational conditions for this partial nitritation treatments using newly designed attach-immobilized reactor with the volumetric loading rate of 0.48 kg-N/㎥/d. were determined to be pH 7.5 to 7.7 at 35℃ experimentally. Anammox bacteria grew inside of the attach-immobilized sludge during the long term treatment of partial nitritation and influent NH4-N was successfully removed to dinitrogen gas only in one reactor. This newly discovered one stage NH4-N removal process was named as Single-Stage Nitrogen Removal Using Anammox and Partial Nitritation (SNAP) process. This SNAP reactor was operated under operational conditions of pH 7.5-7.7, 35℃ and DO 2-3㎎/ℓ, and 60 to 80% of influent NH4-N were removed under NH4-N loading rate of 0.4kgN/㎥/d. Through the DNA analysis of the sludge attached on net type acryl fiber biomass carrier, it was made clear that ammonium oxidizers and anammox bacteria coexisted in the same attach-immobilized sludge on biomass carriers. Favorable cultural condition for the growth of anammox bacteria was created inside attach-immobilized nitrifying sludge. Two kinds of anammox bacteria and two kinds of ammonium oxidizers were detected in the SNAP sludge. Exiting ratios of anammox and ammonium oxidizing bacteria were estimated to be 15% and 8.7%, respectively, based on the obtained clone numbers in the PCR analysis for SNAP sludge. This coexisting condition was confirmed by the confocal scanning electron microscope on the FISH image of SNAP sludge.
Sho Suzuki,Takuji Gotoda,Chika Kusano,Hisatomo Ikehara,Yo Miyakoshi,Kenji Fujii 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.1
Bowel habits affect the quality of life (QOL) of patients with functional gastrointestinal disorders. This study evaluated the effects of reduced form coenzyme Q 10 (ubiquinol) intake on defecation frequency and stool form in patients with daily abdominal symptoms. This was a single-center, prospective, double-blind, randomized control study. Forty-one patients who had the daily symptom of constipation or diarrhea were randomly assigned at a 1:1 ratio to receive either ubiquinol (150 mg/day) or placebo for 12 weeks. Patients completed a daily diary to collect information regarding their numbers of defecations and stool forms according to the Bristol Stool Form (BSF) Scale for 7 days at baseline and 12 weeks. QOL was assessed using the 36-item short-form (SF-36) at baseline and 12 weeks. Twenty-one patients were assigned to the ubiquinol group, and 20 were assigned to the placebo group. At 12 weeks, the mean defecation frequency, compared to baseline, significantly decreased in the ubiquinol group (−0.1 times/day, P = .034) and increased in the placebo group (+0.3 times/day, P = .004). There was no significant change in the 12-week BSF Scale score of the ubiquinol group (+0.2, P = .123), whereas that of the placebo group was increased (+0.5, P < .001). The 12-week general health perception SF-36 score was significantly increased in the ubiquinol group (+3.5, P = .045), whereas there was no significant difference in that score in the placebo group (+1.2, P = .178). In conclusion, taking ubiquinol for 12 weeks decreased defecation frequencies and increased the QOL score, suggesting that ubiquinol may change the bowel habits and improve QOL in patients with abdominal distress.
( Tatsuya Hayashi ),( Satoshi Saitoh ),( Kei Fukuzawa ),( Yoshinori Tsuji ),( Junji Takahashi ),( Yusuke Kawamura ),( Norio Akuta ),( Masahiro Kobayashi ),( Kenji Ikeda ),( Takeshi Fujii ),( Tosiaki M 대한간학회 2017 Gut and Liver Vol.11 No.5
Background/Aims: Noninvasive liver fibrosis evaluation was performed in patients with nonalcoholic fatty liver disease (NAFLD). We used a quantitative method based on the he-patic volume acquired from gadoxetate disodium-enhanced (Gd-EOB-DTPA-enhanced) magnetic resonance imaging (MRI) for diagnosing advanced fibrosis in patients with NAFLD. Methods: A total of 130 patients who were diagnosed with NAFLD and underwent Gd-EOB-DTPA-enhanced MRI were retrospectively included. Histological data were available for 118 patients. Hepatic volumetric parameters, including the left hepatic lobe to right hepatic lobe volume ratio (L/R ratio), were measured. The usefulness of the L/R ratio for diag-nosing fibrosis ≥F3-4 and F4 was assessed using the area under the receiver operating characteristic (AUROC) curve. Multiple regression analysis was performed to identify vari-ables (age, body mass index, serum fibrosis markers, and histological features) that were associated with the L/R ratio. Results: The L/R ratio demonstrated good performance in differentiating advanced fibrosis (AUROC, 0.80; 95% confi-dence interval, 0.72 to 0.88) from cirrhosis (AUROC, 0.87; 95% confidence interval, 0.75 to 0.99). Multiple regression analysis showed that only fibrosis was significantly associat-ed with the L/R ratio (coefficient, 0.121; p<0.0001). Conclu-sions: The L/R ratio, which is not influenced by pathologi-cal parameters other than fibrosis, is useful for diagnosing cirrhosis in patients with NAFLD. (Gut Liver 2017;11:674- 683)