The orphan nuclear receptor SHP acts as a negative regulator in inflammatory signaling triggered by Toll-like receptors

Yuk, Jae-Min,Shin, Dong-Min,Lee, Hye-Mi,Kim, Jwa-Jin,Kim, Sun-Woong,Jin, Hyo Sun,Yang, Chul-Su,Park, Kyeong Ah,Chanda, Dipanjan,Kim, Don-Kyu,Huang, Song Mei,Lee, Sang Ki,Lee, Chul-Ho,Kim, Jin-Man,Song Nature Publishing Group, a division of Macmillan P 2011 Nature immunology Vol.12 No.8

The orphan nuclear receptor SHP (small heterodimer partner) is a transcriptional corepressor that regulates hepatic metabolic pathways. Here we identified a role for SHP as an intrinsic negative regulator of Toll-like receptor (TLR)-triggered inflammatory responses. SHP-deficient mice were more susceptible to endotoxin-induced sepsis. SHP had dual regulatory functions in a canonical transcription factor NF-觀B signaling pathway, acting as both a repressor of transactivation of the NF-觀B subunit p65 and an inhibitor of polyubiquitination of the adaptor TRAF6. SHP-mediated inhibition of signaling via the TLR was mimicked by macrophage-stimulating protein (MSP), a strong inducer of SHP expression, via an AMP-activated protein kinase??dependent signaling pathway. Our data identify a previously unrecognized role for SHP in the regulation of TLR signaling.

Vitamin D3 Induces Autophagy in Human Monocytes/Macrophages via Cathelicidin

Yuk, Jae-Min,Shin, Dong-Min,Lee, Hye-Mi,Yang, Chul-Su,Jin, Hyo Sun,Kim, Kwang-Kyu,Lee, Zee-Won,Lee, Sang-Hee,Kim, Jin-Man,Jo, Eun-Kyeong Elsevier 2009 Cell host & microbe Vol.6 No.3

<P><B>Summary</B></P><P>Autophagy and vitamin D3-mediated innate immunity have been shown to confer protection against infection with intracellular <I>Mycobacterium tuberculosis</I>. Here, we show that these two antimycobacterial defenses are physiologically linked via a regulatory function of human cathelicidin (hCAP-18/LL-37), a member of the cathelicidin family of antimicrobial proteins. We show that 1,25-dihydroxyvitamin D3 (1,25D3), the active form of vitamin D, induced autophagy in human monocytes via cathelicidin, which activated transcription of the autophagy-related genes <I>Beclin-1</I> and <I>Atg5</I>. 1,25D3 also induced the colocalization of mycobacterial phagosomes with autophagosomes in human macrophages in a cathelicidin-dependent manner. Furthermore, the antimycobacterial activity in human macrophages mediated by physiological levels of 1,25D3 required autophagy and cathelicidin. These results indicate that human cathelicidin, a protein that has direct antimicrobial activity, also serves as a mediator of vitamin D3-induced autophagy.</P>

Learning curve of anterior transboturator mesh procedure using the Learning Curve-Cumulative Summation Analysis

( Jin Sung Yuk ),( Chan Hee Jin ),( Kyong Wook Yi ),( Tak Kim ),( Jun Young Hur ),( Sun Haeng Kim ),( Jung Ho Shin ) 대한산부인과학회 2011 대한산부인과학회 학술대회 Vol.97 No.-

Six-year survival of patients with unsuspected uterine malignancy after laparoscopic versus laparotomic myomectomy: An 11-year national retrospective cohort study

Yuk, Jin-Sung,Lee, Jung Hun Elsevier 2018 Gynecologic oncology Vol.151 No.1

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>The primary objective was to compare the overall survival of women with unsuspected uterine malignancy (UUM), including sarcomas and adenosarcomas, diagnosed after laparotomic versus laparoscopic myomectomy. The secondary objective was to determine the incidence of UUM diagnosed after myomectomy.</P> <P><B>Methods</B></P> <P>We analyzed the national health insurance database, which covers almost the entire Korean population, between 2006 and 2010 to calculate the incidence and mortality of UUM diagnosed after myomectomy. Diagnosis and procedure codes were used to identify women with or without UUM.</P> <P><B>Results</B></P> <P>During the study period, 78,826 patients who underwent myomectomy among women in the database (23 million per year) were enrolled. The women were divided into a laparotomic myomectomy group (<I>n</I> = 56,213) and a laparoscopic myomectomy group (<I>n</I> = 22,613). The incidence of UUM diagnosed after myomectomy was 0.08% in both groups (47/56,213 and 18/22,613 women, respectively). There was no difference in mean age, socioeconomic status, diagnostic code, UUM incidence at 5-year intervals, survival rate, or mean survival time. The 5-year survival rates of women with UUM were 95.7% and 88.9% in the laparotomic and laparoscopic groups, respectively. A Kaplan-Meier survival analysis showed no difference in the overall survival rates according to the surgical method (<I>P</I> = 0.447).</P> <P><B>Conclusions</B></P> <P>The incidence of UUM after myomectomy was 0.08% after laparotomic or laparoscopic myomectomy. Although morcellator use does not reduce the overall survival rate, clinicians should explain the risks of intraperitoneal tumor dissemination to patients and do their best to prevent tumor spillage when using this tool.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The incidence of unexpected uterine malignancy after myomectomy was 0.08%. </LI> <LI> Laparotomic and laparoscopic myomectomy had no difference in the incidence. </LI> <LI> Laparoscopy did not deteriorate the prognosis of the unexpected uterine malignancy. </LI> </UL> </P>

Bacillus calmette-guerin cell wall cytoskeleton enhances colon cancer radiosensitivity through autophagy.

Yuk, Jae-Min,Shin, Dong-Min,Song, Kyoung-Sub,Lim, Kyu,Kim, Ki-Hye,Lee, Sang-Hee,Kim, Jin-Man,Lee, Ji-Sook,Paik, Tae-Hyun,Kim, Jun-Sang,Jo, Eun-Kyeong Landes Bioscience 2010 AUTOPHAGY Vol.6 No.1

<P>The cell wall skeleton of Mycobacterium bovis Bacillus Calmette-Guerin (BCG/CWS) is an effective antitumor immunotherapy agent. Here, we demonstrate that BCG/CWS has a radiosensitizing effect on colon cancer cells through the induction of autophagic cell death. Exposure of HCT116 colon cancer cells to BCG/CWS before ionizing radiation (IR) resulted in increased cell death in a caspase-independent manner. Treatment with BCG/CWS plus IR resulted in the induction of autophagy in colon cancer cells. Either the autophagy inhibitor 3-methyladenine or knockdown of beclin 1 or Atg7 significantly reduced tumor cell death induced by BCG/CWS plus IR, whereas the caspase inhibitor z-VAD-fmk failed to do so. BCG/CWS plus IR-mediated autophagy and cell death was mediated predominantly by the generation of reactive oxygen species (ROS). The c-Jun NH(2)-terminal kinase pathway functioned upstream of ROS generation in the induction of autophagy and cell death in HCT116 cells after co-treatment with BCG/CWS and IR. Furthermore, toll-like receptor (TLR) 2, and in part, TLR4, were responsible for BCG/CWS-induced radiosensitization. In vivo studies revealed that BCG/CWS-mediated radiosensitization of HCT116 xenograft growth is accompanied predominantly by autophagy. Our data suggest that BCG/CWS in combination with IR is a promising therapeutic strategy for enhancing radiation therapy in colon cancer cells through the induction of autophagy.</P>

Lelliottia jeotgali sp. nov., isolated from a traditional Korean fermented clam

Yuk, Kyung-Jin,Kim, You-Tae,Huh, Chul-Sung,Lee, Ju-Hoon Microbiology Society 2018 International journal of systematic and evolutiona Vol.68 No.5

Activities of Antioxidant and Redox Enzymes in Human Normal Hepatic and Hepatoma Cell Lines

Yuk-Young Lee,Hong-Gyum Kim,Haeng-Im Jung,Youn Hee Shin,Sung Min Hong,박은희,Jae Hoon Sa,Chang Jin Lim 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.2

The cellular defense system (including glutathione, glutathione-related enzymes, antioxidant and redox enzymes) plays a crucial role in cell survival and growth in aerobic organisms. To understand its physiological role in tumor cells, the glutathione con-tent and related enzyme activities in the human nor-mal hepatic cell line, Chang and human hepatoma cell line, HepG2, were systematically measured and com-pared. Superoxide dismutase, catalase, and glutathione peroxidase activities are 2.8-, 4.3-, and 2.9-fold higher in HepG2 cells than in Chang cells. Total glutathione content is also about 1.4-fold higher in HepG2, which is supported by significant increases in γ-glutamylcy-steine synthetase and glutathione synthetase activities. Two other glutathione-related enzymes, glutathione reductase and γ-glutamyltranspeptidase, are up-regulated in HepG2 cells. However, thioredoxin reduc-tase and glutathione S-transferase activities are sig-nificantly lower in HepG2 cells. These results propose that defense-related enzymes are largely modulated in tumor cells, which might be linked to their growth and maintenance.

Comparison of single-dose and non-single-dose protocol of methotrexate in treatment of ectopic pregnancy: a systematic review and meta-analysis

( Jin-sung Yuk ),( Won I. Park ),( Jung Hun Lee ),( Hyeong Sik Ahn ),( Hyun Jung Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-

목적: The purpose of this study was to compare treatment success rates, side effects rates and time to resolution between single-dose protocol and non-single-dose protocol (multi-dose protocol and two-dose protocol) of methotrexate (MTX) in treatment of ectopic pregnancy (EP) using randomized controlled trials (RCTs). 방법: We searched MEDLINE, EMBASE, SCOPUS, Web of Science, the Cochrane Central Register of Controlled Trials, ClinicalTrials.gov, International Clinical Trials Registry Platform and EU Clinical Trials Register. The relative risk (RR) and 95% confidence intervals (CIs) were used for calculating dichotomous outcomes, while mean difference (MD) and 95% CIs were used for calculating continuous outcomes. 결과: We selected 9 RCTs among total 1521 articles. The success rate of single-dose protocol was similar to that of non-single-dose protocol in treating EP [RR (95% CI): 1.02 (0.98~1.06; 9 trials, 891 patients), I2=0%]. Although non-single-dose protocol tended to have a higher side effects compared to single-dose protocol, there was no significant difference in both group, [RR (95% CI): 0.76 (0.58~1.01; 7 trials, 704 patients), I2=12%]. However, in subgroup analysis about tubal pregnancy, non-single dose protocol appeared to have a higher side effects rate than single-dose protocol [RR (95% CI): 0.69 (0.51~0.93; 3 trials, 369 patients), I2=0%]. Single-dose protocol had longer resolution time than non-single-dose protocol [MD (95% CI): 4.5 (0.40~8.60; 5 trials, 528 patients), I2=83%]. 결론: Single-dose protocol has similar success rates to non-single-dose protocol in treating EP, and tends to lower side effects rate than non-single- dose protocol. Also, in treating tubal pregnancy, single-dose protocol has lower side effects rate than non-single-dose protocol. However, single- dose protocol has longer resolution time than non-single-dose protocol.

Association between pregnancy and acute appendicitis in South Korea

Jin-Sung Yuk,Yong Jin Kim,Jun-Young Hur,Jung-Ho Shin 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.85 No.2

Purpose: To estimate the prevalence of acute appendicitis and the relationship between pregnancy and acute appendicitis among South Korean women in 2009. Methods: This was a cross-sectional study over 1 year period using a national registry data. We analyzed a national patient sample (n = 1,116,040) from a database complied by the South Korean National Health Insurance in 2009. Results: We identified 15,974 cases of acute appendicitis from 2009. The prevalence rate of acute appendicitis was 228 ± 2 per 100,000 persons. The prevalence in men was higher than in women. The peak prevalence of the disease in both genders occurred in patients aged 10 to 14 years. After that, prevalence declined with age. The prevalence of acute appendicitis in women aged 20 to 39 years was negatively associated with age and pregnancy (P < 0.001) but was not associated with socioeconomic status. The prevalence of perforated appendicitis cases by age is represented by a U-shaped curve. The prevalence was highest in people less than five years of age and in people older than 60 years. Conclusion: We found that the prevalence of acute appendicitis decreased with increasing age after early teens, and that the prevalence of acute appendicitis in pregnant women was lower than in nonpregnant women.

One-way membrane for transdermal drug delivery systems : Ⅱ. System optimization

Yuk, Soon Hong,Lee, Seung Jin,Okano, Teruo,Berner, Bret,Kim, Sung Wan 梨花女子大學校 藥學硏究所 1992 藥學硏究論文集 Vol.- No.2

Effective flux of steroid solute through skin can be achieved by the use of ethanol as a co-solvent and penetration enhancer. To maintain constant drug release from a reservoir-type transdermal device and permeation through the skin, the composition of ethanol in the reservoir must remain constant. Ethanol permeability is much higher than that of either drug or water; any decrease in ethanol content may result in decreased drug flux. In an attempt to design a transdermal delivery device with constant ethanol enhancing activity, a model system was designed with separate ethanol reservoir, drug/solvent reservoir and receiver compartments. The ethanol and drug/solvent reservoir compartments were separated by a polydimethylsiloxane copolymer laminate membrane. This 'one-way' rate-controlling membrane(Yuk et al., Int. J. Pharm., 77(1991) 2210229) optimized ethanol flux while preventing drug and water back flux. Permeation studies with model steroid solutes in this system demonstrated consistent ethanol enhancing activity with constant solute flux across the skin.