Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

Glucose deprivation으로 유발된 Neuro 2A 세포의 산화적 손상에 대한 七氣湯의 보호효과

성기호(Ki-Ho Seong),이정섭(Jung-Sup Lee),신선호(Sun-Ho Shin) 대한한의학회 2010 대한한의학회지 Vol.31 No.2

중부지역 옥수수 파종기에 따른 생육단계 및 수량성 변화

서종호, 문중경, 정건호, 서민정, 구자환, 박장환, 배환희, 신성휴, 권영업 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Maize hybrids were planted on April 1, April 25, May 20 and June 15 in 2012, 2013 at the experimental paddy and upland fields in Suwon city , respectively to know changes of growth stage and yield according to different planting time. Maize planting on April 1 was not recommended because total growing days at planting on April 1 was not different with that at planting on April 25 due to many days from planting to emergence over 20 days at planting on April 1. Total growing days were not much different among three planting time after April 25 because days from silking to maturity was lengthened though days from planting to silking was shortened at later planting time. Stalk weight was decreased as planting time was delayed, particularly at planting times after May 20 at paddy field by excess water injury during early growth stage. But, grain yield was not low as in May and June. It is needed to introduce early maturity maize variety as Yanganok at late planting for higher grain yield and enough time for soil preparation for next winter crops

권역단위 농촌지역개발사업의 주민역량 측정을 위한 설문도구개발

리신호 ( Shin Ho Rhee ),민흥기 ( Heung Gi Min ),윤성수 ( Sung Soo Yoon ),정남수 ( Nam Su Jung ),장우석 ( Woo Seok Chang ) 한국농촌계획학회 2014 농촌계획 Vol.20 No.4

The purpose of this study was to develop survey tools for diagnosis of capacity levels in business promotion of rural residents when performing a rural development project of a regional unit. The cases of previous studies were analyzed to select community capacity indicators related to a rural development project. Five indicators were derived : social capital, consciousness of participation, community spirit, and leadership. Based on the five indicators, measurement items of various capacities were selected and 54 survey items were selected through evaluation of experts twice. The pilot tests were conducted and targeted at Jeonnam song ho-jung village and Gyeongnam Haegeumgang village to identify derived survey items. In addition, descriptive statistic analysis and reliability analysis were conducted. As a result, survey items were corrected by reducing 10 items of the total 54 items. This results showed that using this tool could help us understand capacity levels of rural residents.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

청소년의 스마트폰 중독과 우울 및 불안과의 관계

김경은,김필송,민주연,박수경,신서인,이지은,정해인,조호정,최정원,최정인 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to investigate the relationships between the degree of smart phone addiction among adolescents and their depression and anxiety levels. Method: In this study, 379 high school students were selected using the convenient sampling method. The instruments used for this study were smart phone addiction measure, the CES-D (Center for Epidemiological Studies-Depression Scale), and the STAI (State-Trait Anxiety Inventory). Data were analyzed using the SPSS 20.0 program with descriptive statistics, t-test, ANOVA with Scheffe test, and Pearson’s correlation coefficient. Result: The mean score for smart phone addiction was 40.45±17.27, depression was 36.56±9.58, state anxiety was 44.00±9.61, and trait anxiety was 45.70±9.75. There were significant differences between the degree of smart phone addiction and the following variables: gender (t=-4.953, p<.001), hours of smart phone use per day (F=12.259, p<.001), types of frequently used features (F=3.485, p=.008), and satisfaction level for smart phone (F=5.18, p<.001). There were statistically significant relationships (p<.001) among degree of smart phone addiction, depression level, and state and trait anxiety levels. Conclusion: The results of this study suggested that there was a significant relationship between smart phone addiction and mental health, specifically, depression and anxiety among adolescents. Further research is needed to develop nursing strategies to provide care for adolescents who frequently utilize smart phones.

Streptococcus의 유당분해에 대한 자일리톨의 효과

신강호,양규호,최남기,김선미,오종석 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

자일리톨은 탄소 5개가 있는 탄수화물로 치아우식증을 억제할 목적으로 사용되는 자당 대체물이다. 본 논문은 구강 중요 세균인 Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius에서 유당분해에 대한 자일리톨의 작용을 관찰하고자 하였다. 세균에 대한 유당과 자일리톨의 병합효과를 보기 위하여 분광광도계를 이용한 흡광도를 측정하였고 생균수 검사를 실시하였다. Streptococcus mutans 또는 β-galactosidase에 의한 유당분해에 대한 자일리톨 효과를 보기 위하여 thin layer chromatography와 lactose-PTS activity test를 실시하여 다음과 같은 결과를 얻었다. 1. 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus mutans를 배양하면 배양액 흡광도가 배양 8시간에 증가하지 않다가 배양 24시간에 증가하였다. 배양 8시간에서의 생균수도 유당 단독보다 유당과 자일리톨 병합 첨가 시 적게 나타났다. 2. 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus oralis, Streptococcus salivarius를 배양하면 배양액 흡광도가 배양 8시간에 증가하지 않다가 배양 24시간에 증가하였다. 3. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에 Streptococcus mutans를 8시간 배양하면 thin layer chromatography상 남아있는 유당이 많았으나, 배양 24시간에는 모든 유당이 분해되었다. 4. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에서 Streptococcus mutans의 lactose-PTS 활성도가 낮았다. 5. 배지에 유당 단독으로 첨가할 때보다도 유당과 자일리톨을 병합 첨가한 배지에 β-galactosidase 사용시 thin layer chromatography상 유당이 많았다. 이상의 결과는 자일리톨이 Streptococcus의 유당 이용을 억제함을 시사하였다. Xylitol is a 5-carbons carbohydrate, which can be replaced with sucrose for preventing dental caries. To study the effect of xylitol on the fermentation of lactose in bacteria, the important oral bacteria such as Streptococcus (S.) mutans, S. oralis and S. salivarius were studied. The optical density using spectophotometer and the cell concentration were assessed to evaluate the combined effect of lactose and xylitol against the bacteria. Thin layer chromatography and lactose-PTS activity test were performed to evaluate the effect of xylitol on the fermentation of lactose in S. mutans and by β-galactosidase with the following results. 1. The optical density of Streptococcus mutans culture was not increased for 8 hours-incubation in the media added with lactose and xylitol, but was increased at 24 hours-incubation. The number of viable cells at 8 hours-incubation was smaller in the media containing lactose and xylitol in comparison with lactose only. 2. The optical densities of Streptococcus oralis culture and Streptococcus salivarius culture were not increased for 8 hours-incubation in the media added with lactose and xylitol, but were increased at 24 hours-incubation. 3. When Streptococcus mutans was incubated for 8 hours in the media added with lactose and xylitol. the amount of remained lactose was larger compared with the media added with lactose only. But all lactose was fermented in both media after 24 hours-incubation. 4. When Streptococcus mutans was incubated in the media added with lactose and xylitol, the activity of lactose-PTS was higher compared with the media added with lactose only. 5. When β-galactosidase was incubated in the media added with lactose and xylitol, the amount of remained lactose was larger compared with the media added with lactose only. These results indicated that xylitol inhibited the fermentation of lactose by Streptococcus.

소아의 구강내에서 검출된 Streptococcus viridans에 대한 항균제 감수성 연구

신상훈,송정호 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

A large number of streptococci that do not fit readily into any of the established classification schemes have been relegated to a large heterogeneous group called the Streptococcus viridans, which are members of the normal flora of the mucous membranes of the body, including the oral cavity, the nasopharynx, and genitourinary tract. This group includes S. mitis, S. oralis, S. sanguis, S. salivarius, S. milleri, etc. Surveying on the literature, it has been reported that infective endocarditis, meningitis, rhabdomyolysis, cholangitis, appendicitis caused by Streptococcus viridans, which were the most important pathogen in children with malignant hematologic disease. Various antibiotics has been chosen for treatment or prophylaxis for these infections, but were generally lower antimicrobial susceptibilities because of an abuse of antibiotics and advent of resistant group. Therefore, surveillant culture must be performed to evaluate personal antimicrobial susceptibilities of intraoral microbes for proper antimicrobial choice for dental procedures . This study examined sampling from subgingival plaque of 60 chidren's microbes. The cultured bacterial isolates, Streptococcus viridans were examined 10 antimicrobial drugs with the Kirby-Bauer agar disk diffusion method. The used drugs were Penicillin, Ampicillin, Oxacillin, Cephalothin, Imipenem, Gentamicin, Erythromycin , Vancomycin , Ciprofloxacin , Clindamycin. The results were as follows : 1.Sampling Streptococcus viridans were S. mitis(65%), S. oralis(22%), S. sanguis(5%), S. intermedius(3%), S. salivarius(2%), S acidominimus(2%), Unidentified streptococcus(2%). 2.The antimicrobial susceptibility of total Streptococcus viridans : Oxacillin< Erythromycin< Pencillin=Ciprofloxacin< Cephalothin<Ampicillin< Clindamycin< Gentamicin< Imipenem =Vancomycin. 3.The antimicrobial susceptibility of S. mitis : Oxacillin=Erythromycin< Ciprofloxacin< Cephalothin<Penicillin = Ampicillin< Gentamicin< Clidamycin< Imipenem = Vancomycin. 4.The antimicrobial susceptibility of S. oralis : Oxacillin< Erythromycin< Penicillin=Ciprofloxacin=Clindamycin< Cephalothin = Gentamicin< Ampicillin< Imipenem =Vancomycin. 5.There was no significant difference in the antimicrobial susceptibility among each Streptococcus viridans group.

건식경량벽체의 내충격성능 평가방법에 관한 연구 (Ⅰ) : 인간이 벽체에 가하는 충격하중의 분석을 위한 실험 및 고찰 An Experiment to Analyze the Human Impact Load Applied to Framed Walls

신윤호,안정업,김대희,최수경 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

The purpose of this study is to establish an evaluation method of fracture of light-weight framed walls by human impact load. In this paper, part (I), we analyzed human impact load applied to framed walls. At first, we made original measuring equipment of impact load, and carried out the experiment on human impact load in various action. After the experiment, we extracted the essential characteristics. And grasped the property of human impact load systematically based on the relation between maximum load and load velocity.

인쇄매체를 이용한 보건교육자료의 가시성에 영향을 주는 요인에 관한 연구

신준호,정은경,이정애,오승진,김신월 全南大醫大 2001 전남의대학술지 Vol.37 No.2