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Application of temperature-controlled metabolic chamber in mice
Su In Jang,Seul Gi Yoon,Hye Sun Go,Yong Jae Kim,Hak Su Kim,Ji Hyeon Baek,Tae Ho Kim,Ji Min Choi,Soo Kyung Kang,Da In On,Hyun A Noh,Il Yong Kim,Je Kyung Seong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Recently, research on various metabolic diseases such as obesity and diabetes has been in the spotlight. A representative example of this is the study of metabolic function analysis using Metabolic cage. It is believed that these studies will greatly contribute to the prevention and treatment of various metabolic diseases in humans. This study focuses on the study of obesity in mice. Mouse metabolic phenotyping has been widely used for detecting metabolic disorder in genetically engineered mouse. Korea Mouse Phenotyping Center (KMPC), nation-wide program for mouse production and phenotyping in Korea has been establishing several pipelines for disease-specific mouse phenotyping to support the mouse research. KMPC has been providing mouse metabolic phenotyping including high fat diet, exercise and cold challenges. Multiple parameter including energy expenditure (EE), O2/CO2 consumption (RER), heat generation and activity has been provided with histology service and body composition. Also temperature can be measured during metabolic chamber with telemetric system. Brown adipogenesis is one of key mechanism for developing new candidate drug target for curing obesity and type 2 diabetes. Several factors including cold, adrenergic b3 agonist and exercise are well known factor leading to brown adipogenesis. Here we introduce mouse metabolic phenotyping services with environmental control chamber at KMPC. Temperature-controlled metabolic cage, the climate chambers can control temperatures from 4 °C to 35 °C, while measuring energy consumption using oxygen consumption, carbon dioxide production, and respiratory exchange ratio and food intake. Using this, exposing the mouse to a low temperature environment can activate BAT by browning WAT, which causes various diseases such as heart disease, diabetes, and obesity. The changes of metabolic parameter was finely measured during cold exposure with temperature-controlled metabolic cage.
An ANKRD11 exonic deletion accompanied by a congenital megacolon in an infant with KBG syndrome
Go Hun Seo,Arum Oh,Minji Kang,Eun Na Kim,Ja-Hyun Jang,Dae Yeon Kim,Kyung Mo Kim,Han-Wook Yoo,Beom Hee Lee 대한의학유전학회 2019 대한의학유전학회지 Vol.16 No.1
KBG syndrome is an autosomal dominant syndrome presenting with macrodontia, distinctive facial features, skeletal anoma-lies, and neurological problems caused by mutations in the ankyrin repeat domain 11 (ANKRD11) gene. The diagnosis of KBG is difἀcult in very young infants as the characteristic macrodontia and typical facial features are not obvious. The youngest patient diagnosed to date was almost one year of age. We here describe a 2-month-old Korean boy with distinctive craniofa-cial features but without any evidence of macrodontia due to his very early age. He also had a congenital megacolon without ganglion cells in the rectum. A de novo deletion of exons 5-9 of the ANKRD11 gene was identiἀed in this patient by exome sequencing and real-time genomic polymerase chain reaction. As ANKRD11 is involved in the development of myenteric plexus, a bowel movement disorder including a congenital megacolon is not surprising in a patient with KBG syndrome and has possibly been overlooked in past cases.
Go, Min-Jin,Hwang, Joo-Yeon,Kim, Dong-Joon,Lee, Hye-Ja,Jang, Han-Byul,Park, Kyung-Hee,Song, Ji-Hyun,Lee, Jong-Young Korea Genome Organization 2012 Genomics & informatics Vol.10 No.2
Dyslipidemia, mainly characterized by high triglyceride (TG) and low high-density lipoprotein cholesterol (HDL-C) levels, is an important etiological factor in the development of cardiovascular disease (CVD). Considering the relationship between childhood obesity and CVD risk, it would be worthwhile to evaluate whether previously identified lipid-related variants in adult subjects are associated with lipid variations in a childhood obesity study (n = 482). In an association analysis for 16 genome-wide association study (GWAS)-based candidate loci, we confirmed significant associations of a genetic predisposition to lipoprotein concentrations in a childhood obesity study. Having two loci (rs10503669 at LPL and rs16940212 at LIPC) that showed the strongest association with blood levels of TG and HDL-C, we calculated a genetic risk score (GRS), representing the sum of the risk alleles. It has been observed that increasing GRS is significantly associated with decreased HDL-C (effect size, $-1.13{\pm}0.07$) compared to single nucleotide polymorphism combinations without two risk variants. In addition, a positive correlation was observed between allelic dosage score and risk allele (rs10503669 at LPL) on high TG levels (effect size, $10.89{\pm}0.84$). These two loci yielded consistent associations in our previous meta-analysis. Taken together, our findings demonstrate that the genetic architecture of circulating lipid levels (TG and HDL-C) overlap to a large extent in childhood as well as in adulthood. Post-GWAS functional characterization of these variants is further required to elucidate their pathophysiological roles and biological mechanisms.
Jang, Soobin,Kim, Kyeong Han,Sun, Seung-Ho,Go, Ho-Yeon,Lee, Eun-Kyung,Jang, Bo-Hyoung,Shin, Yong-Cheol,Ko, Seong-Gyu Hindawi 2017 Evidence-based Complementary and Alternative Medic Vol.2017 No.-
<P><I>Background.</I> This survey aimed to investigate the characteristics of users and nonusers of herbal medicine and the adverse events experienced due to herbal medicines in South Korea.<I> Methods.</I> The questionnaire consisted of safety, using experience, using type, usage and nonusage reason, purchase location, and adverse events of herbal medicine. The survey was administered by online.<I> Results.</I> Of the total 1,134 respondents, 726 (64.0%) considered herbal medicine safe, and 693 (61.1%) answered that they have taken herbal medicines within the past year. Most common place to purchase them was “TKM hospital or clinic” (63.6%), and most participants (72.2%) took a decoction from a TKM institution. The biggest reason for taking them was for “health improvement” (57.3%), and the reasons for not using them was “medication not necessary” (63.7%). Among those who took herbal medicines, 46 experienced adverse events, and the most frequently reported symptoms were digestive disorders (52.2%). Of the 46 participants who experienced adverse events, 20 (43.5%) were treated by TKM doctors.<I> Conclusions.</I> This study suggests that regulation of herbal medicines is needed in order to resolve problems related to the safety of herbal medicines. </P>
( Go Eun Yang ),( Dong Sun Park ),( Sun Hee Lee ),( Dae Kwon Bae ),( Yun Hui Yang ),( Jang Been Kyung ),( Da Jeong Kim ),( Ehn Kyoung Choi ),( Jin Tae Hong ),( Heon Sang Jeong ),( Hee Jung Kim ),( Su 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.6
The neuroprotective effects of a butanol fraction of white rose petal extract (WRPE-BF) were investigated in a middle cerebral artery occlusion (MCAO) model. Seven week-old male rats were orally administered WRPE-BF for 2 weeks and subjected to MCAO for 2 h, followed by reperfusion. Twenty-four h later, MCAO-induced behavioral dysfunctions were markedly improved in a dose-dependent manner by pretreatment with WRPE-BF. Moreover, higher dose of WRPE-BF not only decreased infarction area but also effectively reduced astrogliosis. The expression of inducible nitric oxide synthase, cyclooxygenase-2, and glial fi brillary acidic protein in MCAO model were markedly inhibited by WRPE-BF treatment. Notably, WRPE-BF decreased nitric oxide and malondialdehyde levels in the striatum and subventricular zone of stroke-challenged brains. These data suggested that WRPE-BF may exert its neuroprotective effects via anti-oxidative and anti-infl ammatory activities against ischemia-reperfusion brain injury and could be a good candidate as a therapeutic target for ischemic stroke.
높은 홀딩 전압으로 인한 래치업 면역을 갖는 양방향 구조의 ESD 보호회로에 관한 연구
정장한(Jang-Han Jung),도경일(Kyung-Il Do),진승후(Seung-Hoo Jin),고경진(Kyung-Jin Go),구용서(Yong-Seo Koo) 한국전기전자학회 2021 전기전자학회논문지 Vol.25 No.2
본 논문에서는 일반적인 SCR의 구조를 개선하여 높은 홀딩 전압으로 인한 래치 업면역 특성을 가지는 새로운 ESD 보호회로를 제안한다. 제안된 ESD회로의 특성검증을 위하여 Synopsys사의 TCAD를 이용하여 시뮬레이션을 진행하였으며, 기존 ESD 보호회로와 비교하여 제시하였다. 또한 설계변수 D1을 이용하여 전기적 특성의 변화를 검증하였다. 시뮬레이션 수행 결과 제안된 ESD 보호회로는 기존의 ESD 보호회로에 비해 높은 홀딩 전압특성과 양방향 방전특성을 확인하였다. 또한, Samsung의 0.13㎛ BCD 공정을 이용하여 설계 후 TLP 측정을 통해 전기적 특성을 검증하였다. 이러한 과정을 통해 본 논문에서 제안된 ESD 보호회로 설계변수의 최적화를 진행하였고 향상된 홀딩 전압으로 래치 업 면역을 갖는다는 점에서 고전압 어플리케이션에 적용하기에 매우 적합함을 검증하였다. In this paper, we propose a novel ESD protection device with Latch-up immunity properties due to high holding voltages by improving the structure of a typical SCR. To verify the characteristics of the proposed ESD circuit, simulations were conducted using Synopsys TCAD and presented compared to existing ESD protection circuits. Furthermore, the variation of electrical properties was verified using the design variable D1. Simulation results confirm that the proposed ESD protective circuit has higher holding voltage properties and bidirectional discharge properties compared to conventional ESD protective circuits. We validate the electrical properties with post-design TLP measurements using Samsung’s 0.13㎛ BCD process. And we verify that the proposed ESD protection circuit in this paper is well suited for high voltage applications in that it has a latch-up immunity due to improved holding voltage through optimization of design variables.